ISArsp6

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnArsp6
Accession numberTranspositionOriginHost
NC_008538 ND Arthrobacter sp.
Arthrobacter sp. FB24 plasmid 1
Arthrobacter sp. FB24 plasmid 2
Arthrobacter sp. FB24 plasmid 3
DNA section
IS Length : 26712 bp

Ends


IR Length : 107/117

IRL : GGGGTGACGTGTGAAAAAGTGAAATATCCGACGCTAGGGTATGGCCGGGC
IRR : GGGGTGGCTTGTGAAAAAGTGAAATATCCGACGTTAAGGTTTTGCCGGGC

Insertion site


Left flankDirect repeatRight flankDR Length
ATCACAGAACTCATACCGGCATCGG5
ACATCGTCAATGAAAGGTCCTCA3
CTGGCTGGCCAGGTTCACGGTGGGC5

DNA sequence

GGGGTGACGTGTGAAAAAGTGAAATATCCGACGCTAGGGTATGGCCGGGCGGGGTAGTGGCCCGTATTTGCCGGGTTTGATCTGGTTGGGGCGGGGCCAC
GTGTAGTCGCCGGTGAGCTGTTCCCAGCCCATCGGTGTGCCGCGGTGTAGCCCAACCTGGTCAACATCGTCCATGACCTGACCGGGCGTGGGATCGGTCT
GAAGGTCCTCATCGGAAGGGCGCCGCCATCCGATACCACTAGGGCCTCCGGGAAGCTTGTCTTCGGGCTCCTGGCCGAGTTTGAACGCGAACTCATTTCC
GAGCGTCACGGGGCAAATTCCGGTACGGTGCGGGGATGGATATTGGATATGCGCGGGTCTCAACGGCCAAGCCGGACCTGGGCCCGGCGACTGGATGCTC
TAGTGGCCGTCGGCATCACCAATGTATTCGCCGACAAGATGGGATGGCTCTTACGGGGGCAGCGGGACGACTGTGCGGTGCTTGCCGCCTGGAGTGAGTA
GCGGTGGCCCGGCGTCCCTGGTGATGGTGACGTTGCCCAGCCCATGACTGGTCAACGCGCGGGTGATTTCGGACCGGGCGGATTCCCATACGTTCTCAGG
ATCCGCACCGGCGGCGGGCTGCAGCCGGAGGCTGAGGCTCTGGGGTCCTGTCTGGATGATCTGGCCCTTACTCAGGCCCTGCACCCTGTCGAGGGCGGTA
CTCAGCGTAAGGGGTGTCACTGCGACGGCGAGCCGGTTGCCTTGGAGGGTCAGCAGCTCGGCGTTCCGTCCCTGCACGTGGATGGCCGGCGTCGGGTCCC
CGCACCGGCAGGGGTCAGTCCTTGCGGTGACGCTGTCGCCGAGGTCGTAGCGCAGGATGGGTTGGATGCGGTTGGCCAGGTTGCTCAGCAGCACGGTGTG
CGAGGGCTGCCCGGGCGGGACGGGTTCATGGTTTGCATCCACCGGTTCCAGGATGGCCCAGTCGCTGTTGACGTGCAGCCAGCCTTCCTGGCAGCTGTAG
CTCAGGAAGGGGCATTCAGTCGCTGCGTAGCCCTGCCGGACCTTCGCCCCAAACGCTCCGCTGATCCTGGCGTACTCTCCCGCAGGCAGTCCCTCGGCCG
AGAGAATAACAGCAACGGGCTGGATGGTCAGACGTCCTGCTTCCTGCTCGGATGCCAGCAACCGGCCAGTGGAGGCGTAGGGGGCGAGGAGGGCCGGGCG
GAAGCTGTTCAGCTGAGCGACGAGGTCCGGTATCGGCGTCTGGACCGGGAACACGCGCACGCTGTGCCGGGCTGGTCCCTTTCGTAGCGCGGCTGCGGCT
GCTGCTGAGGCGAAGTGGCCGCCGGTGGCGACGACCATGGCTATCCGCCCGCGCCCGCGGATGATCCCCATGAGGTCTGCCGGGGTGAGCCAGGAACTGA
GCATGCGGAACGTCAGGGCCTTGGATACGGCCAGGGTTCGGGTGTCGAGCAGGAACAGTCCGCGGGTGCCGGTCGTCCCGGATGTCGTAGCGGCCGTGTA
GCGGTTGAGGAACTTCTCACCGAGCAGCGACGGGTCCGCGACGAACGACTGCGTTCCCTCCAGGGTCACCGCAGGGTCTGTGACCCAGTCATCGAAATGT
GCCATCAGTGCGGCCTTTGTCGTCACGGGCAGCAGACGCGGGTCCGTGATCCGTGCCGGCAGGCCCTGATAGAGCTGGCGGTAGTACGGGGAGCACGTCC
GTGCGTATGTCACCATCTCCACCAGCCGCTCGCGTTGGCGCTGCAGGATCGCAGCGCTGCCCTTTTTCCGGCAGGCGCGGGCATCGAGCAACAACCGGAA
CGGTGTTTCCCTCATGATTCCTCCTGACCCTGGTCTGTGTTCGTCCCAAAAGATTTATGACGGTCAGGCGCCGAACAGCCGGGGCACGACCCCGGAGCTG
ATGAGGATGAACAGGCCCAGCCCGATGAACACCGCAGGGACCAGCCAGTGTTCGATCTTTTCCAGGGTCTCGGTGACTTTCGGGTGGGTGCCTACAACAC
GTCCCAGCGCGCACCAGACGGCGACCAGGATGAGGAATACAACGATGGTGACCACGGTGTCCTGAGGCGCCATGGTGCGGAAGATCGGGGTGTAGATGGC
GATGTTGTCCGCGCCGTTGGCGATGGTGATGCCGGCGACGCTGAGCAGGCCGATCGCACTGATGGCACCTTCTTCGTCCTCTTCCCCGCGTTTGCGCAGG
AAACGGATCAGGCCGATAACCCCGATGGCCAGCGGGACCATGCCGAGCCAGCCGACCCATTCGTCCGGGACAAGCGTCAGCCCAAGGGCGGCCAGGACGC
TGATGCCGACCAGGGTGATGAATCCCAGGTATTGCCCGCCGACGATCTCCCAGGGCCGGGGCCGGCCTTTAGCGGAGGCCAGGAACAGGACGGTCAAAAC
GACGATGTCGTCGATATTCGTGGCGGCGAACATGGCTGCCGCGGCTGCGATGATGCCAAGCATGGGCCCGTGTACCTCTCAGTGTGTCCGGCCGCGGGTT
GCATACCCGCAGGGTGGGTCAGATGGGAGTAGTGAAGGTTCCGATCAGGTTCTGCAGGCTCAGGATCCAGGTGGTCCAGACACCGGAGACCATCAGGATC
CCGACCAGGATCAGGGTCGCGGCTCCTGCGAGATTGAAGGTCCTGATGTTCCGGCGGACGAAGCCCAGGGTGCCGGTGACCCAGCCAAAGCCCCGGGCGA
CGAGGAGGAAGGGGATGCCCAGGCCGATGCAGTAGGCGAAACCGAGCAGCGCCCCGCGCCAGGCCGAGCCGGTGGTGGTGCTCAGCGCCAGGACGGCACT
GAGGGTCGGGCCGATGCAGGGGGTCCAGCCGAGCCCGAATCCGATGCCCAGCAACGGGGCCCCGGCCAGGCCGGTGTTCGGTTTCCAGGACAGTTTCGCC
GTGCTCTGCAGGAACGGGATCTTCCCGGACAGGGCCACACCCATGACGATGACGACCACACCCAGGACACGGGTGATCACATCGCTCCAGCTCACCAGCC
AGAGCCCGATGGTCCCGAATGCCGCACCATACAGGGTGAAAACCGCGGCGAAACCGAGGATGAACAGCCCGACGCCGGCGGTGGTCCGCCGGCGTGCGGC
CTTCTCATCCGTTCCGGCCAGACCGGAGACGTAGCCCAGATAGCCCGGGAGCAGGGGTAGGACACAGGGCGAGATGAACGAGACGAGACCGGCGATGACC
GCCAGTGGCACCGCCAGGAGCAGGGCTCCTGACTGCACACCCTCGGCGAAGAACCCGCCCAGACTCACGTCCCGTTCTCCGGGTCCTTACCGGGCCGCAA
CATGCCCAGCACCAGGACGGCGACGCCGAGCGTGACGAAGACGTCGGCGAGGTTGAACGTCGGGAACCATCCGCTGTGCAGATAATCCACGACCCCGCGC
CCGTCAAGCCGGTCGATGAAGTTCCCGACGGCGCCGCCGGCAACAAGGATTCCACCCGCAAAGGAGGTCCTGGACATCTTCGGGGCGCTGACCGTCAGCC
AGGACAGCAGCGCGGCGATGATGACCCCGGTACCGGCCACGACAAAACCGGTGGGCAGGGTCGCGCCGAGGCTGAAGGCAACCCCGGTGTTGTACAGGAG
CTTGATTGTCAGCAGCGGGGTTTCGATTACCTCTCCCCGGGAGAGGCCTGCCTCGGCCTGGGCCTTGATCAGCAAGTCGGCTCCGGCGAGCAGTAGCGCG
CCGAGCAGCAGCCCGCCCCGCCGTATCCACGGCCGGGACGGGGCACTGTCCCGGACCGGTGCCGCTTGGCTCACCGGGCCGGTTCCAGGTTGGGGACGGC
GTCCCGGGAAACCGGGATGGTGGCGTACTTGCTGACCTTGCCGGCGCGAACGCCGTTGGCGATGACAACGATTTCGGCCAATTCATGAATCAGGACCACG
GCTGCCAGCCCCAGGATGCCGAACAGGGCCAGCGGGATCAGCACCGCGATCAGGGCCAGGGACAGGCCCACGTTCTGGAACATGATCCGGCGGGTACGGC
GGGCGTGCTCCAGTACCTGGGGCAGGTGGTGCAGGTCCTCGCCCATCAGGGCGATGTCGGCGGTTTCGATGGCCACGTCGGTGCCCATCGCGCCCATCGC
GATGCCGGTGTCGGCGGTCGCCAGGGCCGGGGCGTCGTTCACGCCGTCCCCGACCATGGCGGTCGGCTGCCGCTCCTTCAGGGTGCGGATGATGTCCGCC
TTGTCTTCGGGGCGCAGGTCGGCGTGGACTTCGGTGATGCCCGCTGCCTTGCCCAGGGCCTCTGCGGTGAGCCGGTTATCCCCGGTGAGCATGGCGGTGG
TGTAGCCGGCGCGGTTCAGGCGTTCAATGACGGCCCTGGCTTCGGGGCGGAGTTCGTCCCGTACGGCGACGGCACCGATGAGGACAGATTGCTCCTCGAC
CAGGACCGCTGTCGCGCCGCCGGCCTGCATCCGCCGGACGGCTTCCTTCAGCTCCCCGGGGGCGATCCATCCGGGCCTGCCGAGCCGGGCACTGTGCCCG
TCAATGGTCCCTTCGAGCCCCGCACCGGGAACCGTGTTCAGGTCGGTGACGGTGACCCGGTCCGTGGTGGCGGCGAGGATGGCCCGGGCGAGCGGGTGTT
CGCTGCGTTCTTCCAGGCCGGCGGCGACGGCCAGCACCCGTTCCCGGGTGGAGGAGGCGGTGGCCGCGACCTCGATCACGGCGGGTTTGTTCCGGGTCAG
GGTCCCCGTCTTGTCCAGGGCGATGGTGCGGATTTTGCCCAGGGTTTCCAGCGCGCCGCCGCCCTTGATCAGGACGCCCATCCGGCTGGCCGCGCCGACG
GCGGCGACCACGGTCACGGGCACGGAGATGGCAAGGGCACACGGCGAGGCTGCCACCAGGACAACGAGGGCGCGTTCGATCCACAGGACCGGTTCGCCCA
CGATGAAACCGAAGACGGCAATGAGCGCGGCGGCGATCAGGATCCCGGGGACAAGCTTTTTGGCGATGGAGTCGGCCAGGCGCTGGCCGGGGCCCTTGCG
GGACTGCTCGGCCTCAACTATGTGCACGATCCGGGCCAGGGAGTTGTTCTCCGCGGTGCTGGTGACCTGGACCTCGAGCGGACCGGTGCCGTTGATCGAC
CCCGCGTAGACTTCGCTGCCGGGTCCGGCCTCGACAGGGACTGATTCGCCGGTCAGGGCTGAGGTGTCCAGGGAGGTGCGCCCGGTCAGGACGCGTCCGT
CGGTGGCAAGACGCTCGCCCGGGCGAACGACCATGGTCTCACCCAGCACCAGCTCGGACGGGGAGACGGTAACTTCGGTTCCGTTGCGCAGCACCCTGGC
CTCGGACGGGACCAGGTCCAGCAGCGCCCGCAGCCCGCGCCGGGTGCGTGCCACCGAGTATTCTTCCAGGCCCTCGGAGATGGCATAGAGGAAGGCCAGC
ATCGCCGCTTCCTCGATCTGGCCCAGGATGACGGCGCCCACGGCCGCGATCGTCATCAGCGTGCCGACACCGATCTTGCCCTTGACCAGCCGGCGCAGGG
TGGAGGGAACGAACGTCCACGCTGCGACCAGCAGCGCCGCGGCCTCCAGCGGCAGGGTGACCCACTCGGATGCGTCGAGCAGCGAAGCAATCCACGCCAC
CAGCAGCAGGACTCCGGAGACCGCGGCCGCGCGGACCTCGGTCACCTGCCAGAAGCCCTCGGCTTCTTCCTGGCCTTCTTCTGTTTCCGGCTTATCATCG
CCGCATCCGCACGCGTCGCTCACAGTTGCTCCTCTTTGGTGGTTTCGGCGTCGCGGGATGCGTCGCCGTAGGTCGGGCACAGGCTCACTGCGTTGCCGGT
GGCGGCCAAAAGGATCTCGGCCTGGGCCAGGACATCGATCAGTTCCGGACGGGTCAGGGAATAGAACACCTTCCGGCCTTCCGTCCGGCCCGTGACCAGG
CCGCAGTCCCGCAGGCAGGCCACGTGCGCGGAGACAGTGGACTGCGCCAGACCCAGTACCTGGGTCAGGTCACCGACACGGGCCTCGCCCCGGGCGATAC
GCTTTACGATGGCAAGCCGGGCGGGGTCGGCCAGGGAATGGAACAACGCGGCGGCCGGTTCCAGCCGGGTGCCACTTCTCGTATCAATCGTCATACACCG
ATGATATCCAAAGTCGGTGATATGTGCCAGCCGCAACCTTCATGGCGTTCCGCGTGCAGGTCCGGGGCCAGCGCGCTTATGGCGCCGCGGCAGGGGTATG
TGCGGAGCCGTGTCAGTGCGGTCCGGGGCGTCCTGAGAGGATGAAACCGCTTCGAGGCAGCGCGGGCATCCGGGTAAGGTCGATGCTCAGGTCCTGGGCG
GGTACGCTCATGCCGGTCTCCGCGGCCAGTGCCCGGACGGCCCGCCGCATCAGGTCAACGGTGATGTCCTCACCGGGGCAGCGATGCCCCGCGGCAGGGT
CGCCGGCGCCCTGGGGAACCAGGGTGTGGGGGTCCGGCCGCCAGGCGCGGAACCGGGCAGGGTCGAAGCTCTCCGGATCCTTCCAGATCCGGCCGTCGTG
GTTCGTGCCGTACAGATCCAGCAGCACCCATTGACCCTCTTTGAATGTCTGGCCCTTCCATTGCAGTGGTTGGCGGGCCGTGCCGCCGACGAAGGGGAAG
AACGGGTAATACCGGCGGACTTCCTGTGCGAAGCAGTCCAGGTCTGCGTCCTGGCCTGCGTTCAGGATGTCTTTCCATTCGGGGTGCTGCTGCAGGGCGA
CGGCTGCGAAGACCATGAAGCGGCTGACGGCCTCGATGGGCCGGAGCAGGTTGAGAAGTTCCACCGCCGCGGTGTCAACCGGGAGTGCGTTGCCGTGTTC
GTCGGTGTGAAAGGCGATCGACGCGGCCGGCGTGTCCCTGGCCGCGTCCGGGCCGGACCGGCGGATGGTGTCGAGGCAGTCGGCGGCCCATTTTTCGGTT
CCGCGGCGGCGCCACCGCGCATACCAGTTCGCCGGCCCGACCGCGCCGGCCTTTTCGATCATCAGGCTCAGTTCCCGGGCGCGGCGGCCCACTGTAGCCT
GGTCTGCCGGCACTCCGGCCCATTCGCAGGCGGCTGCGGTGAGGATCCGGCGCAGCTCGTCATGGAGTACCACCGCGCCCGCGCCACGCCACCGCTCCGC
CGCGGAGCGCCATTCGGTGTCGAAAGCCTGGCCGAGACGGTCCACCGATTCCTTGGTCATCAGGTCACGAAACAGCTGTTTCCGGTGCCGGTGGGCGGGC
CCTTCGAGGGACTGCACGCTGCCGGCGTCCTGCAGCAGGTGCTGGGCAGAGCGGGGCATGGCCCTTGCGGCCGAACCGGCCACCGCCGTAGAAGAACTCG
GCCGCCTCCGCCCCGCGAAGGCAGATCACCGGGCGCAGCATCAGCCGGGTGCGGAACGCATCGGTGCCGAACCGGTCACACCGGCTCGATATGAACGTGT
ACCCCTCACGCAGAAGAGCCAAGGAACCCTCAGGCAACTGCACTGTCATGACGGACCAGCCTTCTCTCGTTCCGCTCCGGCATGGCCGGAATTCCGGGTG
TTCGCATGCGGCCGGAGCGTCCGGGAACCCCCGCGTCCCGGCTTTTCCTAGGCCTCGAGTGCGGAAGTGATCAGGGCTTTGAGGGTGCTCTTGTCCAGTC
CACCCAGGATTCTCGCCGCAACTCGCCCTTCCTTGTCCAGGACGAGGGTGGTCGGGACCGCCTGGGGCGGGACGAAGCGGCTCATGGCGAGCAGTACCTT
CCCGTCCTTGTCCTGCACGCTCGGATACGTCACGCCGAAGGTCCGCTCAAAGGCTTCCGCCGTCGGCTTCTCATCCCGGACGTTAATCCCGAGGAACTTC
ACTCCCTGCGGGGAGAACTCCTGGTACAGCGATTCCAGGTGCGGGGCCTCGACACGGCACGGGGCGCAGGCTGCGTACCAGAAATTGAGCACTGTGACCG
CGCCCGCCCAGTCACCGGAACTGACGGGCGTGCCGTCATAAAGGTTCGCGTTCAAGGCCACCGGAGCGCCGCGGGACCCCTCGGCGTATTCCTGCACCGA
TCCGTCACCGGCAATGTAGTTCTTGTTATCCCCCGCCGCCGCCTGCTGGACGAGCGGATCGGGTCCGGCGCAGGCGGCCAAGCCAAGGGAGGCCACAGCC
GCCAGGCCCGTAAAGATGCTGCGCCGGGTCAGGCTGCCGCCGCCGGATGGAGTGTTCATCAAATGGCTTCCTCCTATGACGTGGATGGTGGTCCTGGACG
TGCCGTTTTCACGCCACGCCCAGGCTCAGGTTTCAACCGGAATCGGTTCAATCGTCTTCAGACGATATTATCAGGAAGGGGCGTGGCGAAGCGCCGGCCA
CCCACTTGTACTAATTCTACGTAAGGTAGAACTGCGGGGTTGCAGACCTCACCCGCCCTTGACACCCCGACTGGAAGGCAGCGATGTCCCTACCTGACGC
CCCGGATGCTAAGGCAGCGCCGGTCCTCCCGCCGCTGGGCCCCGCCGGGACGCTGCGCTGGGCGTGGACCCAGCTGACGAGCATGCGGACGGCACTTTTT
CTGCTGCTTTTGCTGGCCGTGGTCGCGGTGCCGGGCTCGTTGTTCCCGCAGCGCCCGGCTAACCCTGCCGTGGTGACCCAGTACATCAAGGACCGTCCGG
AGTACGGGAAAATCCTTGACTCCCTTCAGCTCTTCGATGTCTATTCCTCGGCGTGGTTCTCCGCGATTTACATCCTGTTGTTCATCTCCCTGATCGGCTG
CGTGATACCCCGGGCCCGGGCACACTGGAAGGCCCTGCGTTCAGCCCCGCCGCGGACCCCCCGGCGGCTGTCGCGTCTGCCCGAATACGGCACGCTCGTG
CTGCCGGCAGACGCCGCTGTCGGCCCGGGCGACGCCGTCCGGGACGCAGCCGCAGTACTGAAGAAACGCGGCTACCGCGTCGACATCCGCGACGCCGACG
GCGCGATGCCCTCCCTCGGAGCCGAACGGGGGCTGCTGCGGGAAGTCGGCAACCTCCTCTTCCACACCGCCCTGATCGGCGTGCTGGTCAGCGTCGCGGT
CGGCGGGTTGTTCGGCTACCGGGGCCAAAAAATCATCATCGAAGGCGACACGTTCGTCAACACCCTCGTCGGCTACGACAACTTCACCCCCGGCACCAAC
TTCCAGACCTCCTGGCTGGATCCCTTCGCCGTCACCCTGGACAAGTTCGACGTCCGTTTCGACCGCGAATCCACCCGGCAGTTCGGCCAGCCCATCGATT
TCAAAGCCTCCCTGACGACCCGGAACAGCCCGGACGCGGCCCCGCAGCAGCAGGTCCTGAAAGTCAACGAACCGGTCTACTTCGGCGGAACCGGCATCTT
CCTCGTCGGCAACGGTTACGCACCCATCGTCACCGTCAAAGACGGGGACGGGAACACCGCGTTCAGCGGCCCTGTCGTATCCGTCCCCAACGACGCCGTG
TACACCTCCACCATTGTCCTCAAGGTCCCGGACGCCTCGCCCTCCCAGCTGGGTTTCGTCGGGTTCTTCCTGCCCTCGGCCATCAAGAACCAGGATGGTG
TGTCCTACAGCTTCGACCCGGACCCGCTGAACCCGCAACTGAACCTGAACTCCTACTACGGTGATCTCGGGCTCGATACAGGCAAACCCCAGAACGTCTA
CAACCTGGACGTAAAGACCTTGACCCAGCTCAACGGCCGGGATCTGCCGGCCGGCGGGATTGTCCTGGACGCCGGGCAGAGCTACACCCTCCCGGAGGGC
AAAGGCTCGATCAGCTTTGACGGGCTCAAACGCTACATCGGGGTGGACATCCGCACAGTCCCGGGCCAGGACGGGGTCCTGATCTTCTCCCTGATTGCCG
TCGCCGGGCTGATCGTGTCCCTGTACGTGAACCGCCGCCGCGTCTGGCTCCGCGCCGGCTCCCACGAGGACGGCCGCACCATGATCGAATACGGGCTGCT
GGCCCGCGGCGAAGACCACCGGCTCGCACCCGAGGCCGCGGCCATCCGCGCCCGGCTCATGGCCCGCTGGAACCTCGCCGAGCCCCACGCGACCGACGCC
GCCAACGTCCCTGCCCGCTCACCGAAGGAGCACTAATGCCGGACATCAACGAAACCATGGGCCAATACAGCGAGCTGTTCATGCTCCTGGCCGCAGGCAC
CTACACCGTGGCGTTCATCGCCTTCGCCTGGGACCTGGCCCGCAGCAGCAAGGTCCTGCGCGCGGTGGACCTCAAAGCCGCAGAGGCGGCCCCTGCACGT
GCCGGCGTTCCGGTCAGCGCCGGAATCAGCGCCGCCGCTTCCGGTGACCGTCTGGCCTCCCACGTCGGCGGCCCGGCCGGCATAGCCGAACGCCCGAGCT
CAACCGCCCGCCGAGCGGGAACGGATGCGGCCGGCGCAGGCCAAACTGCCGAACCCGGCATGCGCTACGCCCCCGGGCGGCGCGTCCCGGCCCGGGTGGC
GGTGGCCCTCACGATACTCGGCGCCCTGATCCACGGCGCCGGTGTGGTCACCCGTGCGTTCGGCGCGGGCCGGGTGCCATGGGGCAACATGTACGAGTTC
CTTACCACCGGCGCCTTCGTGGCCGTGGCGGTATTCCTGCTCGCGCTGACCCGGCGCGACCTGCGCTTCCTGGGCACCTTCGTGATCGGTCTGGCCATCA
TCATGCAGGTGGCCGCCTCGATCGCCTACTGGACGCCCGTGGGGCACCTCGTTCCGGCGCTGCAGAGCTACTGGCTGATCATCCATGTCTCCATCGCCGT
ACTGTCCTCGGCACTGTTCACCCTGACCTTCGCGATGTCCGCCCTGCAGCTGGTCCAGTCCCACCGCCAGAAAACCGTAGCTGCCGGAGGCGCGGACAGG
CTCGGGTTCATGCGTCTGGTTCCCTCGGCGCTCAGCCTGGAGAACCTCTCCTACCGCATCAACGCGCTCGGGTTCATCGGCTGGACCTTCACCCTGATGT
TCGGGGCCATCTGGGCCGAAAAGGCCTGGGGCCGCTTCTGGGGCTGGGACACCAAGGAAGTCTGGACCTTCGTCATCTGGGTGGTCTACGCCGGCTACCT
CCATGCCCGCGCCACGCGCGGCTGGACCGGCACCCGCGCCGCCTGGCTGTCCATCGTCGGCTACCTGTGCGTGGTGTTCAACTTCACCATCGTGAACCAG
TTCTTCAACGGCCTGCACTCCTACTCCGGCCTGTGACGTCCTCACAGCTGCCCGGGCGTACCAACCTGCCGCGGCGGGACCAGGGCATGCTGCTTTCGGC
GGCCGCCGTCCTGTGGCCGCTTCCTGACACCTACTTTCAGTGAGCTCGAACCATGCAATCGTTCTCCACGCAGGAGATCTTCCTGCCTTCACCAACGCTG
AGCGCCGTCGCGCTCGGCCCCCTGACGATCCGCTTCTACGCGCTCTGCATCCTCGCGGGCATCGCTGTCGGCACCTGGCTGACCGTCCGCCGTCTCAAGG
CCAGGGGCGGGACCTCGTCACAGGCACTGGACATTGTGATGTGGGCCGTCCCCTTCGGCATCGTCGGCGGCCGGCTCTACCATGTGATCACCGACAACCA
GCTCTACTTCGGGCCCGGTAAGGACCCCTGGGGTGCCCTGCGGATCTGGGAAGGCGGCCTTGGCATCTGGGGTGCGGTTGCCCTCGGGCTGGTCGGGGCA
GCTATCGGTGCCCGCCGCGCCGGAGTCCCCTTCGCCGCGTTCGCTGACGCCGCCGCTCCCGGACTGCTCCTGGCGCAGGCCCTGGGCAGGTGGGGAAACT
GGTTCAACAACGAAATCTACGGCGCACCCACGGACCTGCCATGGAAACTGCAAATCCACACCATGGACCCAACCACAGGACAGGCAGTGACCACTCCAGA
AGGGACCCCTGAGGTCATCGGGTACTTCCAGCCGACCTTCCTCTACGAGTCCCTCTGGTGCCTGGCCGCGGCGGCACTGCTGATATTCCTGGACCGCAAA
TATACGCTCGGAGCCGGAGCGGTCTTCTCCCTCTACATTGTTTTCTACACGGCCGGCCGGTTTGCCTTCGAACTGATGCGCTCCGATGAAGCGAACACGA
TTCTGGGTGCGCGCGTCAACACCTGGGTCGCCGGAGTTGTCTTCATCGCCGGGCTGGCCTTCTTCCAACAGCTCAGATCCCGTACCCGGTCAAAGGTGGA
AACGAACGGCACGCTGCCGGACCCGGCCCGCTAAAGACCCGGAGGCCGCAATCGCTCCGCACATCATGCCAACGGAACAACCACCCGTCTGGGAAAGACC
CCTGAGGATCCGTATCAGGCATAATATCGGGACAGGACTCACATCCGGGAGCCGTGTAGATGCGGGGGCTTCATGGGCAAGGCACACTGGCCACCACGGC
GACCTTCGGGGCTCCTGTGCTCCCCCTAGGTGCGGGCAACGCCGCTCCCTCAATCACGGACTCGCCCCTCAATTCGCTCCATCGGGAGGAAAACCATGAC
CTATGACCTCGTAATCCTCGGCGGCGGCAGCGCCGGCTACGCCGCCGCGCTGCGCGGGGCGCAGCTGGGCATGACGGTGGCCCTGATTGAGGGCGACAAG
CTTGGCGGGACCTGCCTGCACCGTGGATGCATCCCGACCAAGGCGCTGTTGCATTCAGCAGAAGTGGCCGACACCATCCGGGAGAGCGAAGCATTTGGCG
TGGAGAGCGCCTTCGGCCGCGTCGACATGGCCGGGGTGACAAAGTTCAAGGCGAGCGTCGTTGACCGCCTCTATAAAGGACTCCAAGGCCTCGTTTCTTC
CCGCAGCGTCGACCTGATCCAGGGCTGGGGTACGCTGGCCGCCGCAGACACGGTAGAAGTCGACGGCACCAGCTACCGGGGCAAAAACATTGTGCTGGCC
ACCGGGTCCTATTCGAAATCCCTGCCCGGCCTGGACATCAGCGGACGGGTGATCACCTCCGAACAGGCACTCGAAATGGATTTCGTCCCCAAGAGCGCAC
TCATCCTCGGCGGCGGCGTGATCGGTGTGGAATTCGCCTCGGTCTGGGCTTCCTTCGGTACCGAAGTAACCATCATCGAAGCCCTTCCGCGCCTCATCGC
CAACGAGGACGAATCCCTGTCCAAAGGCCTCCAGCGGGCCTTCACGAAACGCGGCATTAAGTTCCTCACCAACACCATGTTCGCCGGTGTCTCCCAGAAC
GATGACGGCGTCACCGTCACCACCCAGGACGACAAAACCCTGGAGGCCGAAGTACTGCTCGTGGCCGTGGGCCGCGGCCCCGTGACAGCGAAGCTAGGCT
ACGAGGACGCCGGCATCCCCATGGAACGCGGCTTCGTGCCCACCAACGACCGGCTGCACACCGGTGTCGGCAACGTCTACGCCATCGGGGACATCGTGCC
CGGCCTTCAGCTGGCCCACCGCGGCTTCCAGCAAGGCATCTTCGTCGCCGAAGAAATCGCCGGTCTGAGCCCGGCACCCATCATCGAATCCGGCATCCCG
CGCGTGACCTACTCCGAGCCCCAGGCCGGCTCTGTCGGCCTCACCGAGGCCCAGGCGAAGGAACAGTTCAGTGCCGACGGCATCGAGACGGTCGAGTACA
ACCTCGGCGGGAACGCCAAAAGCCAGATGCTGCAGACCGCGGGCTTCATCAAACTCATCCGCCAAAAGGAGGGCCCGATCATCGGCGTCCACATGCTCGG
CGCCCGGGTCAGCGAGCTCATCGGCGAAGGCCAGCTCATGGTCAACTGGGAAGCCTATCCCGAGGACGTCGCCAGCCTCCTCCACGCCCACCCCACCCAG
AACGACGCCATCGGCGAAGCGGCCCTCGCCCTGGCCGGCAAGCCCCTGCACGCCCACGGCTGACCGGCGCTTCCTGGCTGGGCGTTCAAGGTGGTTGATT
GAAGCGGAGCCCGGGACGGCGCGCCTGTGCTTAGGCGGGCTGCCGGTTGGGTTCCTTGGGCACGATCAGGGGAAGGAACTGGACGTAGAGGACCATGCCG
ATCAGTTCGACGAGAGTTTGGGTGACGACGACCAGCGCGGCCAAGGCCAGCGGCCCGGGAAGCGCCAGCGCCAGCGGCAGCACAACCAGCGAATTTCGGG
TAGCGCCACTGAAGACAACGGCACGGGTGGACGCAGGATCGAGGCGGGCGCCTTTGGCCGCCAACAGTCCCAGCGGAACCATCACCAGCAGGAAAGCCAC
ATAGACCGGAACCACCACAAGCAGCGAGCCAAGTTCCCTGCTCACTCCTGTGATCTGTGAACCGACTACCACGGTCAGCGTGACCATCATGAGGGGAACC
ATCGAAGCCTGCATGGCCGCCATGACGGTCCGGCCCAGGTTTGTCCGGCGGGCCAGCGCCTGGGTCAGGGCCGCTGCGGCCAGGGGAATGATGATCAGAA
CGATCAGGGCCTCGACAAACGGTGCCGGGTCAATAGCCCCAGCCACGTCGGGGCCGATGAAAACCCACAGGAACACCGGTAGCAGGAGCATCTGCACGAA
CATCAATACCGGAGCGGCGGCCAGGAGCCGGTCACTGGCCCCTCCGGCCAAACCGGTGAAGACAATGACGTAGTCGATGCACGGCGTCAGGAGTACGAGC
ATGACGCCGACCACCAGCGCCGGATCTCCGGCGACAAACCGAGTCAGACCAAACACCACAGCGGGAACGACGACGAAGTTCAGGACCAGCACTGTCGCCA
GGAACCGCAGATCCCTGACTGCCGCGCCGATGGAGGCAAAGGGAACCCCGAGAAAAGTCGCATAGAGCAGCAGTCCGAGGGCCGGGTAAATCGAGCGCTC
CAGCGCCCCCACCGAGCCGGGAGCAAGAAAGCCGAGGGCAACGCCGACAGCGATGGCCGCAAGGTACAGGCCGATCTGATGACGCTCCACCCACGCGGGG
AGATTGCGGGTTTTCACCGTACCCATGCTAGATGATGCGCTTCCTGCGTCTCCCGGTGGCGAGGAACCCTGCAGCAGCTGAGCGAAGGCGGCAGGCTCGT
TCAGCCGGGTGGTGGCATCCGCGGGCAAGACAGCTGGTCCCTGGGTCTTCGGGACAAATTATTCATTGACGAGGACGGGTACGCCTACTGCCGTTGAAAC
GGCTTTGGCCTTAATCTCGGCCGGTACGGGAATGCTGAACTCTTCACGTTCTTCCGGCGCTCCGGAGTGGCTGTGTCCTTGGCTGCCGAGGTGGTGGACG
AAACGGGCGGTTCCGACCATGGTGCCCCGGTCATCGTAGAACCCGGCGAGTATCTGGAGATCGAGCAGTTTGCTGACATCACCGGTGATCCTGACCGCTC
CTGTGGCTGCCTGACCGTCGAAGGTCAGGCCTTCAAAGACCAGCCTGTCATCAAAGGGGCCCCGGACATGGAGGACCTGGCCGGGGGCCGGCCGGGTCGT
CTGGAGTCCGGGCAGGTCCGGTTCACCCTCGGCAGCGGCAAGGCCAGGGAAAGATGTTTTCGCGGCGGCCAGCGGGACCTTGTCGCCGGCCGGAGAGGTA
GAGCCCTGTCTGCCCAGTACGACGAATGCAAGTACTGCGAGGGATGCAGCAACGAGGGTTGCCATCAGGAGAATCCGGGATTTGCGGTTCATGGTGTTCC
TTTGGATGTGCCGTGGCCCGGGGTTCGTTCTTGTCCCCCCGGGCCACGGCCAGTCTTGCAGTTTTAGTTCTCCGCGAAGGTCAGCGCAGGACGCGCAAGG
CGTTGCCCACGATGTCGAACAGATCGGTGTTGTCCACGCTGCCCTGCAACTGTTCTGATCCTGAGCCGTAAGCGTAAACAGGCACGTCGGCACCGGTGTG
GTTGCCCGAAAGGTAGTTCAGCCAGAGACTGGCCTCCTTGGAGCCGTCCTTGACCTCGGCTGGGTCATTCGGGGTCCGGAAGGTGGCCGGGCCGAAGTTC
TTCGGGTCTGCAGCGCCGGCGCCGTTGATGATGCCGCTCGAGCGGGTCGGGTCCTTGACGTTTCCGCCAGGCCGGGTCGGGGTGGAGCTATTGGCGGTGT
TGCCGGAATCGACGTTGGCCGGGGGCCCGGCTGCTTCGGCGTTGGTGAAGGAGCCCTTTTCGATGACGCTGAAGCCGCCTGTTTCATGGTCTGCAGTCAC
GATGACCAAGGTGTTGCCGTCCTTCTTGGCGAAATCCAGGGCAGCGGCGACCGAGTCGTCGAAGGCCTTGATTTCCTCAAGGGCCTGGGCCGCGTCGTTG
GCGTGGGAGCGCTTGTCAATCAAAGCCCCCTCAATCTGCAGGTAGAACCCGTCGCCCTTGGAGTCTTTTTCCTTTTTCTTATCGAGCAGTTCGATGGCCT
TCTTGGTCATCTCCGGCAGGGAGGGCTCCTGCGCCTGAGGCGCGGCGGGGTTGTCCTGCTTGGCTTTCTCGACGGTCAGGTTGCCCTTGTTGAACAAGCC
GAAGACCTTCTCACCGGCGGCCCCGTTCAGGTCCGCGCGGGTGGCGGGGATCTGGCTCGAGACCGAGCCCAGGACCGAATAGCCCTGCTCCTTCAACGCG
GTTTCGTCCGTGGCGTCGAAACGGCTTAAGCCGCCACCAAAGATGACGTCTGCTGTGCCGTTGCGGGCGATCTGGTCCGCAATCGGGGTGACGCGGACGT
CACTGGTCGGCAGCGGGGTGCCGGTGATGCTGGTGTCCTGGCACGCGGCGGCTGAGTAGACAGGACCCTGGCAGCCGCGGGCGAGGGAGTGGCTCATCTG
CGAGGCCGGGGTAGCGTCCGTAATCTCGGCGGTGGAGACGTTGCCTGTGCGGTAGCCGGCCTTCTTGGCCAGTTCCATCATTGTCGGGACCACTGCGCCC
TTGGCGTCCACGCCGAGGGCGGCGTTGTAGGTCTTCACCCCGGAGGCCCAGGCGGTGGCGGCGGAGGCGGAATCGGTGACGAGGTTCGGTTTGAAGTCAG
TCTGACCGGGCTGGCCGGAGTTCTTTTCCACGGCGTAAGTGCTGACGTAGCCCTGGGCCGGAAGGGTTTCCATGGCCAGTTTGCCTGCGGCTCCGTAGAA
GCGTTCACGGCCGGCGGTGACGTGGGTACGGCCCATGCCGTCGCCGAGCAGGTAAATGACGTTCTTGGTCCGGCCGTTGTTCCCCTCCGGGGCGGCGGTG
GCGGGGGCGCTGACAGCGACGGCTGTTAGCACGGCGGCAACCGCCGCCGTTGAAGTAAGCATGGATAGGGTGCGGCGCATCAGGGATCTCCCAAGGAAAA
ACTGCGAACGAGTGAATGAAGGGGTTGGTGCCCGGGTTGAAACCCTAAGAGCGAAACCTTCACATGTCCGGACTTCCCTGTGTCCGGCCGGTAGCCGGGA
GGTGAACATCGATTCTGAGAACAAGATCAACGGTGCGACACGAATCACCGCCGAAGGCCGGATATCTTCACCAATTCTCACGTGGTGGTATGGCGTTTCC
TTTCTTCAGGCGGAGGGGGCCGCGTTTTAGGCGCAGCAGGAGAGTTTGGAGACGTCTGTGGTGAAGGCCTTGCAGGCGATCCTGATGCCCTCGGCCATGG
TCAGGTAGGGGCTCCACATGCCGGCGACCTGCCCGGTGGTCATCCCCGCCTCGAGCAGGTAGACGCCCGCGGCGGCCAGCTCGCCCGCGTCCTTGGCCAC
GGCGGTGAGGCCCACGATCCGGCCGGTGTCACGGTCGGCAACGACTTTGATGAATCCCCGGGTATCGCGGTTGACCAGGGCCCTGGGGACGTAGTTCAGG
GGCAGCACCCGGCAGTCGCAGCGGATACCGGCAGCGACCGCGTCCTGCTCAGTCATCCCGACGGCACCCACAGCCGGGCTGGTGAAGGTCACCCGCGGCA
GATGCCGGTAGTCGACCTCATCCACGGCGTCGGTAAACGCGTTCTCCACGGCAAGGGCGCCGTGGGCCGCGGCGACGTAGACGAACTCACGGTGGCCGGT
GACATCCCCTGCAGCCCAGATCCTGGGGTTCGAGCTGGCCAGACGGGAATCGACCACGATCTCACCGGACGTGCCGGTCTTCACCCCGACGGCGTCGAGG
TTCAGCCCCTCGGTGACGGGCCGGCGCCCCAGGGCCACGAGCAGCCGGGAGGCCCGGAACACTTCCTGTCCACCGGCCACCGAAGCGGTGGCGCTGACCC
CGCCGGTGGCCTCATCGAGGCTCACTGAGTCCACGGTGGCCCGCCGGACCACCCGGATCCCCTCATCCGCGAAGACCCCGGCCAGCGCCTTGGATACTTC
CGGCTCCTCGTGCGAGGTGAGCCTGGAGCGGACGAGCATAGTCACGCGGGAGCCGAGCCGGGAGAACAGCTGCGCCTGCTCTAGGGCGACATAGCCGCCG
CCAAGGACCAGCAACGATTCGGGCACTTCGTCCAGTTCCATCGCCGTGGTCGAGGTCAGGTAGCCGGTCTCGGCCAGTCCGGGAACGGGCGGCGCCCATG
GTGTGGAGCCGGTGGCCACAAGGTAGTGTGCGGCCTCGATCGTCTCAACGGCACCGCCCGGTGAGGTGACCTGGAGCAGAGGTGTGTCCTGGGTCCCGGT
GAATACCGCGTCTCCCTGGAGCATGGCCCAGCCATAGTCCGCGGCAAGGTCGACGTACTTCTCTGTCCGCATGCTTTCAACAAGTGCAGCCTTGCCCTCG
ATGAGGGCCGAAATGTCCACGGCCCCGGCCGAGGCCATCACGCCGGGGAAGCGGGCCGAGTCCAACGCCACGTGCCGGGCGTCCGCGGCAGCCAGGAGCG
CCTTCGACGGCACGCACCCGGTGTTCACGCATGTCCCGCCCAAGGTGCCACGCTCAATCATCACTACCCGCTTGCCCAGCCCCCTGGCCCGGATCGCGGC
CGCAAACGCCGCGCCACCGGAACCGATGATCGCCAGATCGAACCGTTCGTTTCCCATACCGCTGTCCTTCCCAATGCATCAAAAAGCCCTTTGGAACCAT
CATCAACCTTCCAGTACAGTGGAAGGTCAAGTCGATGCATTGCATGAGGGAGAGCGGCGAAGATGAAGATCGGGCAGGTCGCAGAAACAACCGGGACCAC
GACCAAGACCCTGCGGTTCTACGAAGCAGAAGGGCTGCTTCCTCCCGCCGAACGCACCGCCTCCGGCTACCGCGATTACTCAGAAGACGTCGTGGGCCGG
ATCGGTTTCATCCACCGCGGCCAGGCCGCAGGGCTCACCCTCGCCCAGATCAGGCAGATCCTGAACATCCGCGACCGAGGCCAGACGCCGTGCTCGCACG
TCCGGGAATTGCTCGGGCAGCGCTTGACAGAGCTTGATGCCCAGACCACTGAGCTCCTGGCCCTTAGGGAAACGATCTCCCAGCTTTACGCCCAGGCAGC
CGAACCCAAACAACCCGAGGCCTGCCCCCCAGACGAGATCTGCCGCTACCTCTGACCCGTCACCGTCCCGCCGAGGCTACCCGCACTCAAAGACAACGAG
TACCAGGCTGTCACTTTCGTGTTGGAATTGCGACGAAAGTGCAAGCGGCCCGGCCGCATCACGCCCCCCCAGTGGGCCCCCTCAAACTGCGATTTCCGCT
CAAAGTGGGACCGAATCAGATTGACATTCTCAGGAGCCATCTGCACGGAATTTCACACTCGTCTGCACTTGTTCTGGAACGTCGCTGTCAGTTCAGAAGT
CGTCCGCACGAATTTTTCGTCTGCGCAGCCGTCGAGGTCACGGCTGCACCCGTGCGGCCGCGCCGGCACAACCTCCAGGCTTCCTGGTAGGGCTCATTGA
ACGGGGTGCAGACCACGGGCGTGTTCTTGACGGTGTACTCCGGCTGGTTAACGTCGTCGTCCTGAACGCCTTGTGCCCGGCCCCGGCCCCCGTGCCCTAA
GAACGCGCAGCTACCGAAAGGTCCTAGCCTGCGGCCAGCAGGACACCGCACGCGGCTTCGCAGCGGCGGCAGGCTTCGGCACAGATCCGGCAGTGCTCGT
GCATCCCCGCGTGCTTTTGGCACTCTTCGGCGCAGGCGGCGCACGCTGCCCGGCAGGCCTCCAACGCCGCAAGCGTGATGGGACTCTGCCCTCCGGTCTG
CCGGGACAGGAGGTTCCCTGTGGCCGCGCAGACATCGGCGCAGTCCAAATCTGTCCGGATACAGGTGGTGAGCTCGGCGACCATGTCCTCGCCCAGACAC
GCGTCCGCGCAGGCCGTGCATGTCTGGGCGCACTCGAAGCAGGCGGCAATACATCCGGCCAGGCTGTCCCGGCTCATGGCAGGGTCTGCCTGGGGGTGGG
CGTTGAGCATGGCGCTGACGTGGTGGCTCATGGGGTGGCTCCTGTCGCTGGGCGAGGGGGCGGCCGGGCCGTTCCCGGCCGTCCCCCTCACCCTAGACCC
GCCGGCGGTACGCCCGACAGGTCAGGGACAACCCGGAATCACGGGGCTATTCGGCGCCGGCTGGCTGGAACGTCATTCCTCCGTCCGTGGAGGCAACGAT
CCCGTCCGGGGTGGCAGCCCAGATCCGTGGACTGCCGTCGTCGCCCTTGACTGCGGCGATGGCCAGCACCTGGGCCTCGATGCGGCCCTTCTTCGTCCAG
GTCTGTCCGGTGTCAGCGGAGTAGTGCACGGTACCGTTGGGTTCAGCACCTACGGCCTCGGTCGGGTTCGCGAAGGCGGCGAACTGGATCACGGGGCCGC
CTTCCAGCGGCTTCCATGTGGTGCCGGCGTCGGTGGAGCGCTGGATTCCTTCCTGGGTGGTGGCCAGAACGGTGTCACTGCCGGGATGCCCGGCCAGGAC
TGCCGGGGTAAACCCGGCTGACACGGTTTTCCAGGTCTTCCCGTCGGGGCTGGTGCGCAGCGTCCCATCGGAGGCAATGATGCCGGATTTGGTGGTGGTC
ATGGCGTGGAAGTCGGATTCGCCCTGCCGGGAGAGCTGTTCCCAGGTTTTGCCGCCATCAGTGGACTTCAGCAAACCCATCGGGTTGGGCAGGTCCGATC
CGGGCCCAGGGTGGCCGGAGGCGTAAAACGGGCCCTTGTCACCGGCTGTGGTGAAGCCCATCAGGTCGTTGGTGTCACCGATTTTGGTGGCCGGGGACTT
GGTGACGTCGAAGAGGCCGTCGTGGGTGGCCAGCAGCACCTGGCCGGTTGTGCCGTTCACGGTGAGTCCATGGACGTGGGAGCTGGGAAGGCCCGGGGCC
GCAGTGGTCGGTGCGGTGCCGGCCTCCGGCGTATCGGTGGTGGAGCCGGGGGTGCAGGCGGTCAGGGTCAGCAGGAGGGCGGCGGCTGCGGCGAGGGCAG
CCGCTGGACGGGGACGGGTCAAAGAGTGCAGGGGCACGGGGAACTCCAAAGGCTCAACGGGAATGTCTGTGGGAGGGCACCGGCCCCCGGGAAGGGGGCC
GGTGCCGGATGTGGGCTGGCTACAGGGTGGCCAGGAGGTCTTTCATTTCCTGGATTTCGGCTTCCTGGGAGCTCACGATCTCCTTGCTGAGCTGGACGGC
GTCGGCGTTCTTGCCGTCGGTGGTTTCGGTCTTGGCCATCATGACGGCGCCCTCATGGTGGGCGATCATCTGGGTCAGGAACAGCCTGGCGGCCTCGGTG
CCCTGCGCGGCCTCGAGTTTGGCCATGTCGTCCTGGCCCATCATCCCGGACATGCTGTGGGTCGAAGGCATGTTCGTGGGCATCATTGTGGGCTGGCCCC
AGCTTTCCAGCCAGCCGGTCATTTTCTCGATCTCGGGGCCTTGGGCCGCTTTGATCTTGGTGGCAAGGTCTGTCACCGCGGCGGGAATGCCTTCTTTCTT
GAGCATGATGTCGCTCATGGCCACGGCCTGGGTGTGGTGCGGGATCATCATCTGCGCGAACATGGTGTCCGCGTCGTTGAACTCCGCCGAAGCTGCTGCC
GAAGCTGCCGGGGCTGAGCTTGAAGCCGTGCCGGAGCTGCCGTGGTTCATGCCGGACGTGGTGCTTGCCTCCGGACTGCCGGTCCCCGAACCGGCGGAGC
AGCCGGCGAGGGCGATTGCTGCGGCGATGGCGGCAGCGGAAAGGGTCAGGGTCTTCTTCATGAAAATCAGGGTCCTCAAAAGTATCAGCGGCGCTCAGGG
CGCCTTGGACATTGCGCGCAGTGGCCGGCCCGGCCGGAGACCGGACTGTTTCACGCGTGCTGGTGGCTGGACTATGCGCCGGGGAAGGACACGGCGCAAG
CGTGCCGGCTTACGTCCGGCTGATACAGAGCTCGCCCGGGGAAGGGCTGCCCGGTGAGTAGGAATAGTTGGTGCCGACGACGGCCGCGCCAGCGAAATCC
GGCAGCGCGGACGGTGCCGTTCCCGGCATGGGAGCTGTCTGAGGCGTGTTTCCCTGTGACAGCACGCAGGCATTGCCCGTGGCGGACATCTCGGGGCACG
ACCCTGCAGCGGAACAGGAAGACGACAACCCGGTAGCCGCACCGACCACTGGTGCCGCAGCCGGTGACAACCCTGGCCCGTGGCCGGCGTTGGCACCGGT
CGGCATGCCTTCCATCTGCGCCATGGCCGGGGCCGCAGCTGTCACGGAGATGCCATGCTCCGCGGTCATGTTCTGCGCATCGGTCATGATGTGCATGCCG
AAGATTCCGGCGATGATGGCCAGGGCCGCGGTCAGAAATCCTGCACGGAGGAGGAAAGCGGTGACGGGGCCGCGCGATACTGCGGTCACAACGTTTCCTT
CCTTCCGTGCCGGTTCGATAATCCAAGGGTACCGGCCGGGGGTTTCCTCGCAGGTCACGACCATAGGTTTCAACGTGCCAGGGAGGGGTTCAGTTTCACC
ATCCGGTGCCGGTGCTCTACGACTGTCCTTGACCGGAGTCAGGAGTAAGGTATCGGGTTACGCGGGCCCTTGTGCCTGGCCTGAGTGATGCGCGCGGCCT
CGCCGTCGGCGGGGACGGCCGCTTTCCCTGGCAGAGGCTGTGTACCGCGTCGAAGAGTTAGTTGCCGCTAAACGTGTACCCGGCAGAGGTGACCGCCTCA
CGCACCGCAGCTCATTCGAGGCCATCTGTGCGAAAACCGCGAACCCAGCTGTGAAAAAAGGCGGCCATTTGCGGTTAACAGTCACAGCTCGGGCGGCTTG
TGAATTCGTCCATATCCGCCCGGCGCCACCATGTCCGGTAACGATCGTTCACAAGACACTTCGGACCGGTTTTTCGGAACCGCGGAACCACGCGGTCAGC
TTTGTAACGCTTGCCGTGTACGCGGCTGTGTAACGTACAGCAGGGTTAGTGATACGTTTTGGTCATGCGAATCGGATACGGTCGGGTCTCCACCCGTGAC
CAGCACCCCGAAGCCCAACACGACGCCCTGACAGCGGCCGGCTGCGATCAGGTCTACATCGACACAGCCTCCGGAAAGCTCTCCCGCAGACCCGAACTCG
ACAAGGCCCTGCTCTCCGCAAACAGGGAAGGCGACCAGCTCGTCGTCACCAAACTCGACCGGCTCGGGCGGTCCCTGGAGCACCTCATCGACCTGTCCAA
GACGCTGCAGGAACGCGGTGTGGACCTGATCGTCCTGGACCAGGGCATCGACACATCCACCGCCATCGGCCGGATGTTCTTCCAGATCCTCGGATCGATC
GCCGAGTTCGAACACGCCCTCATGTCCGAACGAACCCGGGACGGGCTGGCCGCCGCACGTGCGCGGGGCAGGACCGGCGGACAGAAACCAAAACTCGGCC
CCCGCCAGGTCAGGCTTGCCCGGGAAATGTACGAGGAGAAAAACGCCGACGGGAAACGGGCCCACACCGTCGAGCACATCGCCCAGGAATTCGGCGTCAC
CCGGCCCACCATCTACCGGCACTTGGCGAAGCCATGAGCGGTGGCCGTCCGCTGCTGCAGGTACAGATGCTGACCCCGTGGGGCGGCAACGTAGCGGCAA
AGGAGACATGAGTTGGCTCTGCGTTCTTTGCTCACGGCTGCCGAGCGCGGCCAAATCCTGGCAATGCCCGCTGAAACAGAGGACCTTGCAGCCCACTACA
CGCTCAGTGATGCGGATATGTCGTTGATCCGGCAACGCCGCGGGGACGCGAACAGGCTGGGCTTTGCGGTCCAGCTGTGTCTGCTGCGCCACCCGGGCAT
CGGGCTGGCCGACGACACCGACGTGCCGCCGGAACTCATTGCCTGGCTGGCCTCCAGCCTGGGCGTTTCCATTGATGCCTGGGACGAGTATGGAACACGT
GAGGAAACGCGGCAGGAGCATGGACGGGAGATCCGCGGGTATCTGGGCATGTCAGCGTTCGGCATCGCGGACTACCGCTGGCTCGTGGAGCATGTGGGTG
TTGTGGCTGCGCACACGGACAAGGGCCTGGTCCTGGTCGAAAGCGCGAGGGATTTCCTGCAGGCAAGGAAGGTCGCGTTGCCCGGGATCGGGGTCATTGA
AAAGGCCTGTGCGCAGGCGCTGACCAGGGCCAATAGGCGGATTTACCTCACTTTGGGTGAGCAGCTGACCGTGGGTCACCGGCAACGCCTCGACGGGCTG
CTGCGCCGCCGCCGCGATAGTTCTCTGACGGAAATCGGTTGGCTGCGGCAGGCGCCGCTGCGACCCAACGCGCGGGCGATGAATGAGCACATCGACCGGC
TCACCACCTGGCGTGCGCTGGAGCTGCCCTGGGCTGCGGGACGGCTGGTTCATCGGAACAGGTTGCTGAAGCTTGCCCGGGAGGGTGCCTCGATGACCGC
GGCGGATCTGGCCAGATTTGAACCGGCACGCCGGTACGCGACCCTCTTCGCCATGGCCACCGAAAGCATGGCCACCGTCACCGACGAAATCATCGATCTG
CACGACCGGATCATCGGCCGGCTCATCCGGACCGCACAGAACAAGCAAAACCAGGCCACCCTGGCATCCCGCTCCACCGTCGCCGCCATGATGCGCATCC
ATTCCAGGCTCGGTGATGCCCTCTTTGAGGCCAAGGAAAACGGCGAAGATCCCTTCGCCGCCATCGAAACGGCCATCGGCTGGGAATCCCTGGCCGAAAG
CATCGCCCACGCCAAGGAACTGACCCGCCCGGCCCTCGAGGACCCCCTCGCCCTCGTCAGCGCCCACTTCACCACCCTGCGCCGCTACACCCCGGCATTT
CTTGCCGTCCTTGACCTCAACGCGGCCCCGGCGGCACAGGACCTGCTGGCAGCAATCAACCTCGTCCGCACCCTGAACACCGCCGGAGCCCGAAAAATCC
CCGACGATGCGCCCACCTCGTTCGTCCGGCCCCGGTGGAAGCCGCTGGTCTTCACGGAAAACGGCATAGACCGGGGCTTCTACGAGTTCTGTGCCCTCGC
GGAACTAAAGAATGCGCTTCGTTCCGGAGACTTGTGGGTCACCGGATCCCGTCAATTCCGTGACTTCGATGACTACCTTCTTGCCGGTCCTGACTACACG
GTTATGAAAACCACCGGGAAGCTGCCTCTGGTCACGACCGACGGCGGCGAAAGCTATCTCCAGAACCGGCTGGCCCTGCTCAACGAACGGCTGCACCATG
TCAACGACCTCGCCTCCCGCGATGAACTGCCCGGGGTGATGGTCACGGACAAGGGCGTGAAAATCACCCCGCTGGAGACAATCGTGCCAAAACACGCGCA
GCCACTGATCGATCAGGCAAGCGCAATGTTTCCGCGGATCCGGATCACCGATCTGTTGATGGAAGTTGATGGCTGGACCGGGTTCACCCGCCATTTCACC
AGCCTGAAATCCGGCCAGCCCTCCAAAGACAAGCAACTTCTTCTCACCGCCATCCTCGCGGACGGAATCAACCTGGGCCTGACGAAGATGGCCGAGTCAT
GCACCGGCGTCAGCTACGCCCAGCTGGACCGCCACCAGGCCTCCTACATCCGGGACGAAACCTACAGCGCCGCTCTGGCGGAACTGGTGAACACCCAGCA
CGGACACCCCTTCGCCGCACAGTGGGGCGACGGGACCACCTCCTCATCGGACGGGCAGCGGTTCCGTGCCAGCAGCAAAGCCGAATCCACCGGGCATGTG
AACCCCAAGTACGGTGCCGAGCCCGGCCGGCTGATCTACACACACATCTCGGACCAGTACTCGCCCTTCCACAGCAAGCTCGTCAACGTCGGCGACCGCG
ACGCGACCTACGTCCTGGACGGGCTGCTCTACCACGAGTCCGACCTGGCGATCCAGGAGCACTACACGGATACGGCCGGATTCACCGATCACCTCTTCGC
TCTTATGCACCTGCTCGGGTACCGGTTCGCCCCACGGATCCGCAACATCGGCGACACCCGTCTCTACACACCTACCACCGATCCGGGACTTGCCACGTTG
GCGCCGCTGATCGGCGGGACCATCAACACGAAAATGATTGCCCTGCATTGGGATGAAATCCTCCGCCTCGCCGCGTCCATCAAGACCGGCACCGTGACCG
CGTCCCTGATGATGCGAAAACTCGGCGCCTACCCGCGCCAGAACGGGCTCGCACTCGCGCTGCGGGAGCTGGGCAGACTGGAGCGGACCCTCTTCCTGCT
GGACTGGCTCCAGAACCCCGGCCTGCGCCGCAAAGTCACGGCCGGCCTGAACAAGGGCGAGGCCCGGAACACCCTCGCCCGGGCCGTCTTCTTCAACCGC
CTCGGCGAAATCCGCGACCGCTCCTTCGAACAGCAACGCTACCGCGCCAGCGGACTGAACCTTCTCACCGCGGCCATTATTCTCTGGAACACCGTCTACC
TCGACCGCACCATCACCACCCTCAATAAGGACGGGAACGCCACGGACCCTGACCTGCTGCGGTTCCTCTCACCCCTGGGCTGGGAACACATCAACCTCAC
CGGCGACTACACCTGGCCCCGCGCCAACCAGATCAAACCCGGCAAATACAGGCCACTACGCCGCCCGGCAAAACCTTAACGTCGGATATTTCACTTTTTC
ACAAGCCACCCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
573 bp190 aa2306523637+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MRIGYGRVSTRDQHPEAQHDALTAAGCDQVYIDTASGKLSRRPELDKALLSANREGDQLVVTKLDRLGRSLEHLIDLSKTLQERGVDLIVLDQGIDTSTA
IGRMFFQILGSIAEFEHALMSERTRDGLAAARARGRTGGQKPKLGPRQVRLAREMYEEKNADGKRAHTVEHIAQEFGVTRPTIYRHLAKP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
2967 bp988 aa2371326679+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LALRSLLTAAERGQILAMPAETEDLAAHYTLSDADMSLIRQRRGDANRLGFAVQLCLLRHPGIGLADDTDVPPELIAWLASSLGVSIDAWDEYGTREETR
QEHGREIRGYLGMSAFGIADYRWLVEHVGVVAAHTDKGLVLVESARDFLQARKVALPGIGVIEKACAQALTRANRRIYLTLGEQLTVGHRQRLDGLLRRR
RDSSLTEIGWLRQAPLRPNARAMNEHIDRLTTWRALELPWAAGRLVHRNRLLKLAREGASMTAADLARFEPARRYATLFAMATESMATVTDEIIDLHDRI
IGRLIRTAQNKQNQATLASRSTVAAMMRIHSRLGDALFEAKENGEDPFAAIETAIGWESLAESIAHAKELTRPALEDPLALVSAHFTTLRRYTPAFLAVL
DLNAAPAAQDLLAAINLVRTLNTAGARKIPDDAPTSFVRPRWKPLVFTENGIDRGFYEFCALAELKNALRSGDLWVTGSRQFRDFDDYLLAGPDYTVMKT
TGKLPLVTTDGGESYLQNRLALLNERLHHVNDLASRDELPGVMVTDKGVKITPLETIVPKHAQPLIDQASAMFPRIRITDLLMEVDGWTGFTRHFTSLKS
GQPSKDKQLLLTAILADGINLGLTKMAESCTGVSYAQLDRHQASYIRDETYSAALAELVNTQHGHPFAAQWGDGTTSSSDGQRFRASSKAESTGHVNPKY
GAEPGRLIYTHISDQYSPFHSKLVNVGDRDATYVLDGLLYHESDLAIQEHYTDTAGFTDHLFALMHLLGYRFAPRIRNIGDTRLYTPTTDPGLATLAPLI
GGTINTKMIALHWDEILRLAASIKTGTVTASLMMRKLGAYPRQNGLALALRELGRLERTLFLLDWLQNPGLRRKVTAGLNKGEARNTLARAVFFNRLGEI
RDRSFEQQRYRASGLNLLTAAIILWNTVYLDRTITTLNKDGNATDPDLLRFLSPLGWEHINLTGDYTWPRANQIKPGKYRPLRRPAKP

 

Blast result :
Comments
ISArsp6 ORFB, the transposase, is 74% aa similar to ISSod9.
ISArsp6 ORFA is the resolvase.

Before the resolvase and the transposase, this trasnposon carries one metal opron (23 ORF)
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Thompson,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Beasley,F., Chen,W., Jerke,K., Nakatsu,C.H. and Richardson,P. (2006) Direct submission Genbank