ISfinder Mirror Site

ISAau5

Family ISNCY
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008712	ND	Arthrobacter aurescens	Arthrobacter aurescens TC1 plasmid TC1

DNA section

IS Length : 3989 bp

Ends

IR Length : 40/48

IRL : GGTGGGATTGATCGCGAGAAGGTCAGCGCATTGCTCACTTATGTCAGTAG
IRR : GGTGGGATTGCTCGCGATCTTGTCAGCGGATTGCTCAATTGTGTCAGTGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTGCCACCC		ACGCCATTCG	0

DNA sequence

GGTGGGATTGATCGCGAGAAGGTCAGCGCATTGCTCACTTATGTCAGTAGATTTCTCACCACTGTCAGTGGTCTGATTACCCATGTCAGTGCATGTACGG
TCGCCTGCCGACGTGGCTGGGCGCACGAGCGCAGAGGAGCCCTGGGAGTTGGACGTAGACGAGCCAGTTGTCAGTGCTGACCTGCCGCGGGAGGCGGTGG
TGCGGCTGCTGCTGTCCTTGGACGCCGCGGGGCGGTTAACCACTGAGGATGTGCGCTTGGCCGCGGAAGGATCCGATGTCAGCGAGAGGACCGTGTGGCG
TTGGCTCGCGCAGGCACGGTCCGAAGGGGAAGTACAGTCACCTCGGGAGCGGTTCGAGATTACTGATGAGGTCAGGGAGCGGCTGGCGTTCTGGCGTGGG
AACGTCTCAGCGGTGCATCGGGAACTCGTCGCCGCCGCAGGTGAGGGCGGGAAGCGCCCGAGCCGAGCGACGGTCCAGCGGGCCGTGGCTCGCGACGTTC
TTCGTGGCGAGCTTGCCGGACTACGTGGTGGCGAGCGTGCGCGACGCGCACACGAAGTGTTCCTGCGCCGACCGCGCGTGCACCGCAATGAGGTATGGGA
GGCCGATCACGTTGAGGTGCCGGTCGAAGTGCTCTTGCCCGACGATCGGCTGGTCAAGCCTTGGGTGACGTGGTTCGTCGACGTGGGAACTGACGCGGTG
TGCGGTGCCGCGGTAACGCCGGGTCCACCGAGCCGGGAGAGCATCTTGGCTGCGCTTCGCGCGGCGATCAGTCTGGAGGAGCCCTACGGGCCGCCGGGCG
GATTGCCTGGTGCCGTTCGGATCGACCGCGGCAAGGACTTCCTGTCCAAGACGGTGCACAGCGTCCTGGCCGGGTTGGCGGTGCGGGTGGTGGCACTGCC
CGGGTACACTCCGCACCTGAAGGGCACCGTGGAGTCACTCAACAACGCGGCCGAGACCATGTTCTTCGCTGCCCTACCGAGGTACACCCACGCGCAGCAG
GGTGCGAACGGGAAGCCGACCGATCCCGATGCACCTGCATTGCCGTTCGAGGCGTTCGTTGCCGAGTTCCTTACCTGGGTCCGCTGGTGGAACGGCGAGA
ACGAAGGTCACACCATAGCGGCCTTGGGGGGCCGGACGCCGTTGCAAGCCTGGGCCGAGGATCCGACCCCGCTGAGCACTGTCCCGGCTGCTGACCTGCG
GCTGTTGATGCTGGAGGATGATGGCCGCGCTCGGACGATCACTCCCAAGGGGGTGTCGTGGCGCGGACGGCACTACGTGGCGGCCTGGATGACCGGCCAG
GTTGGTCTGGCGGTCCGAGTGCGTCACATGCCGCACCACGAGCACGAGATCGAGGTTTTTGGCGCGACCAGCGGCAAGCACCTGGGCTCAGCAACTTTGG
CCGATGCTGCCAGCGCGGAGGAGGTCGCGGCACTGCACCGGGCCCGGAGCGAGCAGCAGCGCCGCCTACGAGCCGATCTGCGGGCCGCGGAGAAGGCACG
CAGAGTCCGCTATGCCGCGGCCACCGAGGCGGCGCCGCTCCAACCGGTTCAGGCGGTCACCGCAGAGGCGGCACGAGCCGAGCTCGCCACGGCCCAAGAC
ACCCAATTGCAGGCCGCGGCCCGGGTCGATCTGTTCGCCCCGGGCCCGCCGTCGCCTGAATGGGTACTCCCCCACGCCTCCCGGACCGGCCCGAAGCCAT
GAATGTCTGGGCCGCCGTGGACGGGGCCGAGGACCACTACCTCGCACTTCCCGGAGCGAACGTGGTGGCCACTGATGCATTGTTGGCGCTTCGGGACAAC
CTGGTCGACGTCATCGCGGCTCGGGCGATGATGTGCGTGCACGGTGACGCCGGACTGGGCAAGACGCTGTCAGTCAACGCCGCTCTGCGGGCGCTGGCGC
CGGCCGACGTGTGCCGGGTGCAGTTCCGTGCCCGGCCAACCCCTCGCGATATCCGGCACGTGCTCTTCGAGGCACTCGGAATCGGCGGCAGCCCGCCGAG
TCGTCCGATCGAGTTCGACGCGCTCCTCAAAGACGTTCTCGAAGAACGGTTCCGGGTGCTGGTCTGCGACGAAGCCCAGTGGCTCTCACGCGAATGCTTC
GAGTTGTGGCGACACCTGTGGGACGACCGCCGCACCGACATCGCCATCGTCTTCGTCGGCGGCGGCGACTGCTACCGGGTGCTTCGCCGTGAGCCAATGC
TGTCCAGCCGCGTTTATGTCTGGCAGGAGTTCCGTCGCCTCACCCCTGAGCAGGTACTCGAGGTCATCCCCGCCTACCACCCGATCTGGGCCGATGCGGA
CCCCGCCGATATCGCCTTCGCCGATGCTCACGCCGGGCATGGCAACTTCCGTGCTTGGGCCAAGATCACAGCCCACTCGGTGACCGCGCTCCGTCGCCTT
GACCGTCCGACCGGCACCGCGCCGGACCGGGATGTGCTGCGCTGGGCCTTCAGCCGACTCGGCGGCAGCACCTGAGAACCGGCTTCGATAATCCTCCCAC
CAGCGCCGTCACGCCGAGCCGTCCGGCGGCGGTGCAGGTGCTTCTGGACCGCACCGACGACATCGCGGTCACCGCTGGCTTACTGCGACGGCATAACCCC
GCCGCCGGCCCCGTCGTCGTGCACCCGACCCCAACCGGTACCACGTCCCAGGACCTGAGCCACGACCTGCTGCGCGCCCTGGGAAGACCGATCCATCGGC
TCGCCGCTGAACGCCTGCAGGGCTCCCGGCCCGCCTGGCGCGCCGTGGCCGCGTGGCTTCACGCAGACCAACTCTCCGATCTCATCGTGCTGCGCGCCCA
CTTGCTCGGCGCCGACCGCTGGAGCCAGCTCATCGAGCTGAGCCGCGGCACTGGCACCCGGTTGACCCTGGTCTGCCACACACGGTCGATCCCCAGTCCC
TTGGCTCAGGCGCTTACCGAGATCGACCACCAGATCCTCACCAATCTCGAGCTCCTCGATCTGCCGGCGGGTGGGCACCGACCCAGCGCCCCTCCGGCAG
CCGATCCGGGGATGGCTTCCCTGCCGCGCGTCACGTACCCCGGCGTGGTCAATTTCCGCCGCTACGCTTTCGACCAACTCGGGCCGATCGAGTTCGCCCG
GCTCGATACCATTTACCAGTCGGCCCAGGAGACGACCCTGGCCTGGCTCGATGGCCGCCCCGACGCGGCCACGTCCGACGACGGTCGAGAAGCTGTCATG
GGTCTGCTTTCGGAGCTGGTCCACGACAGTCCCAGTCGCACCCACACGCTGGCCCGGCTTCGCAGCGCGCAGGCCGCTTTCCGGCGGCACGGATTCAAGC
TGACTCTGCCGGCATCCCGGGATTTGCTCGACGTCCTGGCGGGCCCGGGCCTGACCAGCCAACCAGTCACCGACCAGCTACTCGACCGAATCCGCGCAGG
CGTAGCGCACCCAGCGATCGCCGCCGCGTTGGCCACGAGCTTGTTTACCGGCCTGGCGCCGACGAGCTTGATGCCCCTGACGTGGAAGTCGCTGACACCG
GGCGCCGACCGTCTTCGGATCAACAATCGAGTAACGAGGCTGCAACGTCCAGCACCGAACCTGTGGCCCCGGCGAACACTCGTTCGCGTCCCGGCCATCT
TCTACGTGCCGACCGCCGTCCGGCCCCTGTTCCAGGCCGCCCGAGAGTTCATCCCGCCCGACGAGAACATCCGTCGTCGCCTCTTCTGGGGCGCCATCGC
TCACCAGATCGAGGCGGCGGCCTCGGAGTGCGCCATCACGCTGCCCCACCATGTGGCCCCGGTGATCGCCTGGCAGGCGTGGATCGGGATCGAGCGTGTC
GACTATTTCGGCGGCCAGTACGACGTCGACTTCAGCGCCGACCAGCAGTTCCAGCCGCAGCCCACCCCTATCGAGCCCCCAGCGACCTTGGAAAGGTGAG
ACCCGAACGGGATGCCCAACCCTCACCTTCCGCTTGAAGGCACTGACACAATTGAGCAATCCGCTGACAAGATCGCGAGCAATCCCACC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
1503 bp	500 aa	200	1702	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MRLLLSLDAAGRLTTEDVRLAAEGSDVSERTVWRWLAQARSEGEVQSPRERFEITDEVRERLAFWRGNVSAVHRELVAAAGEGGKRPSRATVQRAVARDV
LRGELAGLRGGERARRAHEVFLRRPRVHRNEVWEADHVEVPVEVLLPDDRLVKPWVTWFVDVGTDAVCGAAVTPGPPSRESILAALRAAISLEEPYGPPG
GLPGAVRIDRGKDFLSKTVHSVLAGLAVRVVALPGYTPHLKGTVESLNNAAETMFFAALPRYTHAQQGANGKPTDPDAPALPFEAFVAEFLTWVRWWNGE
NEGHTIAALGGRTPLQAWAEDPTPLSTVPAADLRLLMLEDDGRARTITPKGVSWRGRHYVAAWMTGQVGLAVRVRHMPHHEHEIEVFGATSGKHLGSATL
ADAASAEEVAALHRARSEQQRRLRADLRAAEKARRVRYAAATEAAPLQPVQAVTAEAARAELATAQDTQLQAAARVDLFAPGPPSPEWVLPHASRTGPKP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
759 bp	252 aa	1717	2475	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDGAEDHYLALPGANVVATDALLALRDNLVDVIAARAMMCVHGDAGLGKTLSVNAALRALAPADVCRVQFRARPTPRDIRHVLFEALGIGGSPPSRPIEF
DALLKDVLEERFRVLVCDEAQWLSRECFELWRHLWDDRRTDIAIVFVGGGDCYRVLRREPMLSSRVYVWQEFRRLTPEQVLEVIPAYHPIWADADPADIA
FADAHAGHGNFRAWAKITAHSVTALRRLDRPTGTAPDRDVLRWAFSRLGGST

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1368 bp	455 aa	2532	3899	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MQVLLDRTDDIAVTAGLLRRHNPAAGPVVVHPTPTGTTSQDLSHDLLRALGRPIHRLAAERLQGSRPAWRAVAAWLHADQLSDLIVLRAHLLGADRWSQL
IELSRGTGTRLTLVCHTRSIPSPLAQALTEIDHQILTNLELLDLPAGGHRPSAPPAADPGMASLPRVTYPGVVNFRRYAFDQLGPIEFARLDTIYQSAQE
TTLAWLDGRPDAATSDDGREAVMGLLSELVHDSPSRTHTLARLRSAQAAFRRHGFKLTLPASRDLLDVLAGPGLTSQPVTDQLLDRIRAGVAHPAIAAAL
ATSLFTGLAPTSLMPLTWKSLTPGADRLRINNRVTRLQRPAPNLWPRRTLVRVPAIFYVPTAVRPLFQAAREFIPPDENIRRRLFWGAIAHQIEAAASEC
AITLPHHVAPVIAWQAWIGIERVDYFGGQYDVDFSADQQFQPQPTPIEPPATLER

Blast result :

Comments

ISAau5 ORFA (the Transposase) is 38% aa similar to ISChy3.
ORFB and ORFC are passenger genes.

References

1] ISfinder annotation (2009)
2] Mongodin,E.F., Shapir,N., Daugherty,S.C., DeBoy,R.T., Emerson,J.B., Shvartzbeyn,A., Radune,D., Vamathevan,J., Riggs,F., Grinberg,V., Khouri,H., Wackett,L.P., Nelson,K.E. and Sadowsky,M.J. (2006) PLoS Genet. 2 (12), E214