ISArsp4

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008537 ND Arthrobacter sp.
Arthrobacter sp. FB24 plasmid 1
DNA section
IS Length : 1400 bp

Ends


IR Length : 24/29

IRL : GGGACTGTGGAAATAACGGTGTATCCGGCTCGACACGCCGGGCGGTAAGC
IRR : GGGACTATCTCATTAACGGTGTATCCGGCGTTTTCATTTAGTAGCTTTCA

Insertion site


Left flankDirect repeatRight flankDR Length
CTCCGCAGACCGGCAATCAT0

DNA sequence

GGGACTGTGGAAATAACGGTGTATCCGGCTCGACACGCCGGGCGGTAAGCCTGGCGGGAAGGAGCCCGATATGGCCGCAACACTTGAGGCTGTGCCGAGT
ACGAAAACTGCTGACAAGAAGACAGAAATATCCGAGGCCGACGCTGCCCGCGAGCTGGTCAGGATGGCGAAGGAGCAGGGCCTGTCCCTGACCGGTCCCG
ACGGGCTGCTCAAGCAGCTTACGAAGACCGTGATCGAGACCGCGCTCGACGAGGAAATGACCGAGCATCTCGGATATGAAAAGCACGATTCGGCCGGCAA
ACAGACTGCCAACTCCCGCAACGGGGTTCGCCCGAAAACGGTGCTCACGGAGACGACCGGCCCGGTGGAGGTGGACGTGCCGCGGGACCGGGCCGGCACG
TTCGAGCCGGTCATCGTCAAGAAGCGCCAGCGCCGCCTGACCGGGGTCGATGAGATGGTCCTCTCGCTCTACGCGAAGGGCCTGACCACGGGAGAAATCA
GTGCGCACTTCGCTGAGATCTACGGCGCGTCCGTGTCGAAGGAGACCGTCTCCCGGATCACCGACCGGGTGTTGGAGGAGATGGCCGCGTGGCAGAACCG
GCCGCTGGACGAGGTCTACGCGGCGATCTTCATCGACGCAATCGTCGTCAAGGTCCGCGACGGGCAAGTCGCCAACCGGCCCATCTACGCTGCGATCGGC
GTGACTCTGGAGGGCGAGAAGGACATCCTCGGCCTGTGGGCCGGGACCGGCGGAGAGGGCGCGAAGTTCTGGATGAGCGTGCTCACTGACATCAAGAACC
GCGGCGTGAAAGACACCTTCTTCCTAGTCTGCGACGGGCTCAAAGGCCTGCCCGAAGTCGTCACCAACGTTTGGCCGCTGGCGACCGTGCAGACCTGCAT
CATCCACCTGATCCGGAACACCTTCCGGTTGGCCTCGAGACAGGACTGGGACGCCCTCAAACGCGACGTGAAGCCCATCTACACGGCGCCGAATGCTGAG
GCGGCCCGGACGGCGTTGGAGGAACTCGCGGAGCGCTGGGGCAGGAAATACGGGGCGATCGTGCGGCTCTGGGAGAACGCGTGGGAAGAGCTCATCCCGT
TCCTGGACTACGACGTGGAAATCCGGCGCGTGATCTGCTCGACCAACGCCATCGAATCGCTCAACGCCAGATACCGGCGCGCCGTCAAGGCCCGGGGTCA
TCTCCCCACCGAGCAGGCTGCGCTAAAGTGCCTCTATCTGGTCACCCGATCCCTCGATCCGACCGGTGCCGGCAAGACCCGATGGGCCGTGCGTTGGAAG
CCCGCTTTGAACGCCTTCGCCATCACTTTCAGCGACCGATTCCCGGCAGCTGAAAGCTACTAAATGAAAACGCCGGATACACCGTTAATGAGATAGTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1293 bp430 aa711363+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAATLEAVPSTKTADKKTEISEADAARELVRMAKEQGLSLTGPDGLLKQLTKTVIETALDEEMTEHLGYEKHDSAGKQTANSRNGVRPKTVLTETTGPVE
VDVPRDRAGTFEPVIVKKRQRRLTGVDEMVLSLYAKGLTTGEISAHFAEIYGASVSKETVSRITDRVLEEMAAWQNRPLDEVYAAIFIDAIVVKVRDGQV
ANRPIYAAIGVTLEGEKDILGLWAGTGGEGAKFWMSVLTDIKNRGVKDTFFLVCDGLKGLPEVVTNVWPLATVQTCIIHLIRNTFRLASRQDWDALKRDV
KPIYTAPNAEAARTALEELAERWGRKYGAIVRLWENAWEELIPFLDYDVEIRRVICSTNAIESLNARYRRAVKARGHLPTEQAALKCLYLVTRSLDPTGA
GKTRWAVRWKPALNAFAITFSDRFPAAESY

 

Blast result :
Comments
ISArsp4 is 85% aa similar to IS1554.
References
1] ISfinder annotation (2009).

2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Thompson,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Beasley,F., Chen,W., Jerke,K., Nakatsu,C.H. and Richardson,P. (2006) Direct submission Genbank.