ISBth167
- Family IS3
- Group IS150
Isoform Synonym(s) ISBth9
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ242517 | ND | Bacillus thuringiensis | Bacillus thuringiensis serovar tenebrionis plasmid pBMB165 |
DNA section
IS Length : 1455 bp
Ends
IR Length : 27/35
IRL : TGAAGTGCACCCCAATTGTTAGACACATAATTAACAATTGGAGGTGCATT
IRR : TGAACTGAACCCAAAAAATTAGACAAATATATTAAGCAGCTACTAAGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACTAAAAAA | ATA | TTTCGTAGTC | 3 |
DNA sequence
TGAAGTGCACCCCAATTGTTAGACACATAATTAACAATTGGAGGTGCATTTTTTATGGCTAAATTTTCTTCAAAAGATAAAATACAAGCAGTAAAACGAT
ATCTAGAAGGTACGGAAGGCGGAAAAACCATTGCTAATTCTATAGGGATTCATCCTAGAGAACTTTATCAATGGATTAAACGGTTTGAATTTTCAGGGGA
AAAGGCGTTTGAAAAACGCTATACAACTTACTCTCTCGAGTATAAACTAGATGTAATTCATTATATGAATGAAAATGGGACATCTCTGAGAGAAACAGCT
GCTTTTTTTAATATTCCTTCTTGCGAAACACTCCGAAAATGGAAAGTAGCTTATGAAACAGAAGGATTAGATGCCCTAAAATCAAAGAAAAAGGGGCGTC
TAACCATGGCCAAAGAAAAAGCTAAGCTACAACATTTAAAACAAAACGAAGTATCTCTTGAAGGTTCTATAGAAGCGCTACAAGCAGAGAATGAACGTCT
GCGTATGGAAAATGACTATTTAAAAAAGTTGAATGCCTTAGTTCAAAAGAAGAAAACATCACAGACCAAGACAAAGCGCAATTGATTTATGAATTAAGGC
ATAAATACAAGGTCGTTGACCTTGTGAAAGTCGCTAATATCGCTCGTAGTACGTATTATTACTGGATGAAACAAGCGAAACGTCCAGATAAATATAAGAA
AGTTAAAGAATTAATTAAAGGGATCTTTAGTGAGAATTTTGGGAGGTATGGTTATCGTCGTATTACATTGGAATTACGTAATCGAGGCCATGCATTAAAT
CATAAAACCGTTCGACGTTTAATGAATATCCTGGGATTAAAATGTCTTGTTCGATTAAAAAAATATCGTTCATATAAAGGGACTGTCGGGAAAGTCGCTC
CTAATATTTTAAAACGTAATTTTCACGCATCAAAACCAAATGAAAAATGGGTAACAGATGTGACGGAGTTCCACTTACATGGTAAAAAACTATATCTATC
ACCCATTTTAGACTTATATAACGGAGAAATTATAGCTTACAATATAGAACATAGACCTGCCTATTCCCTTGTTTCTAAAATGTTAAATAAGGCGTTTCAA
CGTTTGAATGATAAGGAAACTCCTATTTTACACTCGGATCAAGGTTGGCATTATCAAATGCGACAATATCATCAGTCGCTTAAAAAACATAACGTTATCC
AAAGTATGTCCCGTAAGGGAAATTGCTTAGATAATGCAGTCATGGAAAATTTCTTTGGCTTATTAAAGTCTGAATTACTTTATCTTAAAGAGTTTGAAAG
TATGGAACAATTTAAGCAAGAACTAGAAACTTATATTCATTACTATAATCACAAAAGAATTAAAACAAAACTAAAAGGATTGTCCCCTGTGCAATACAGA
GTTCAATCCTTAGTAGCTGCTTAATATATTTGTCTAATTTTTTGGGTTCAGTTCA
ATCTAGAAGGTACGGAAGGCGGAAAAACCATTGCTAATTCTATAGGGATTCATCCTAGAGAACTTTATCAATGGATTAAACGGTTTGAATTTTCAGGGGA
AAAGGCGTTTGAAAAACGCTATACAACTTACTCTCTCGAGTATAAACTAGATGTAATTCATTATATGAATGAAAATGGGACATCTCTGAGAGAAACAGCT
GCTTTTTTTAATATTCCTTCTTGCGAAACACTCCGAAAATGGAAAGTAGCTTATGAAACAGAAGGATTAGATGCCCTAAAATCAAAGAAAAAGGGGCGTC
TAACCATGGCCAAAGAAAAAGCTAAGCTACAACATTTAAAACAAAACGAAGTATCTCTTGAAGGTTCTATAGAAGCGCTACAAGCAGAGAATGAACGTCT
GCGTATGGAAAATGACTATTTAAAAAAGTTGAATGCCTTAGTTCAAAAGAAGAAAACATCACAGACCAAGACAAAGCGCAATTGATTTATGAATTAAGGC
ATAAATACAAGGTCGTTGACCTTGTGAAAGTCGCTAATATCGCTCGTAGTACGTATTATTACTGGATGAAACAAGCGAAACGTCCAGATAAATATAAGAA
AGTTAAAGAATTAATTAAAGGGATCTTTAGTGAGAATTTTGGGAGGTATGGTTATCGTCGTATTACATTGGAATTACGTAATCGAGGCCATGCATTAAAT
CATAAAACCGTTCGACGTTTAATGAATATCCTGGGATTAAAATGTCTTGTTCGATTAAAAAAATATCGTTCATATAAAGGGACTGTCGGGAAAGTCGCTC
CTAATATTTTAAAACGTAATTTTCACGCATCAAAACCAAATGAAAAATGGGTAACAGATGTGACGGAGTTCCACTTACATGGTAAAAAACTATATCTATC
ACCCATTTTAGACTTATATAACGGAGAAATTATAGCTTACAATATAGAACATAGACCTGCCTATTCCCTTGTTTCTAAAATGTTAAATAAGGCGTTTCAA
CGTTTGAATGATAAGGAAACTCCTATTTTACACTCGGATCAAGGTTGGCATTATCAAATGCGACAATATCATCAGTCGCTTAAAAAACATAACGTTATCC
AAAGTATGTCCCGTAAGGGAAATTGCTTAGATAATGCAGTCATGGAAAATTTCTTTGGCTTATTAAAGTCTGAATTACTTTATCTTAAAGAGTTTGAAAG
TATGGAACAATTTAAGCAAGAACTAGAAACTTATATTCATTACTATAATCACAAAAGAATTAAAACAAAACTAAAAGGATTGTCCCCTGTGCAATACAGA
GTTCAATCCTTAGTAGCTGCTTAATATATTTGTCTAATTTTTTGGGTTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 531 bp | 176 aa | 55 | 585 | + | No |
Description : First part of the transposase
ORF sequence :
MAKFSSKDKIQAVKRYLEGTEGGKTIANSIGIHPRELYQWIKRFEFSGEKAFEKRYTTYSLEYKLDVIHYMNENGTSLRETAAFFNIPSCETLRKWKVAY
ETEGLDALKSKKKGRLTMAKEKAKLQHLKQNEVSLEGSIEALQAENERLRMENDYLKKLNALVQKKKTSQTKTKRN
ETEGLDALKSKKKGRLTMAKEKAKLQHLKQNEVSLEGSIEALQAENERLRMENDYLKKLNALVQKKKTSQTKTKRN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 516 | 1424 | + | No |
Description : Second part of the transposase
ORF sequence :
LFKKVECLSSKEENITDQDKAQLIYELRHKYKVVDLVKVANIARSTYYYWMKQAKRPDKYKKVKELIKGIFSENFGRYGYRRITLELRNRGHALNHKTVR
RLMNILGLKCLVRLKKYRSYKGTVGKVAPNILKRNFHASKPNEKWVTDVTEFHLHGKKLYLSPILDLYNGEIIAYNIEHRPAYSLVSKMLNKAFQRLNDK
ETPILHSDQGWHYQMRQYHQSLKKHNVIQSMSRKGNCLDNAVMENFFGLLKSELLYLKEFESMEQFKQELETYIHYYNHKRIKTKLKGLSPVQYRVQSLV
AA
RLMNILGLKCLVRLKKYRSYKGTVGKVAPNILKRNFHASKPNEKWVTDVTEFHLHGKKLYLSPILDLYNGEIIAYNIEHRPAYSLVSKMLNKAFQRLNDK
ETPILHSDQGWHYQMRQYHQSLKKHNVIQSMSRKGNCLDNAVMENFFGLLKSELLYLKEFESMEQFKQELETYIHYYNHKRIKTKLKGLSPVQYRVQSLV
AA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1370 bp | 456 aa | 55 | 1424 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKFSSKDKIQAVKRYLEGTEGGKTIANSIGIHPRELYQWIKRFEFSGEKAFEKRYTTYSLEYKLDVIHYMNENGTSLRETAAFFNIPSCETLRKWKVAY
ETEGLDALKSKKKGRLTMAKEKAKLQHLKQNEVSLEGSIEALQAENERLRMENDYLKKVECLSSKEENITDQDKAQLIYELRHKYKVVDLVKVANIARST
YYYWMKQAKRPDKYKKVKELIKGIFSENFGRYGYRRITLELRNRGHALNHKTVRRLMNILGLKCLVRLKKYRSYKGTVGKVAPNILKRNFHASKPNEKWV
TDVTEFHLHGKKLYLSPILDLYNGEIIAYNIEHRPAYSLVSKMLNKAFQRLNDKETPILHSDQGWHYQMRQYHQSLKKHNVIQSMSRKGNCLDNAVMENF
FGLLKSELLYLKEFESMEQFKQELETYIHYYNHKRIKTKLKGLSPVQYRVQSLVAA
ETEGLDALKSKKKGRLTMAKEKAKLQHLKQNEVSLEGSIEALQAENERLRMENDYLKKVECLSSKEENITDQDKAQLIYELRHKYKVVDLVKVANIARST
YYYWMKQAKRPDKYKKVKELIKGIFSENFGRYGYRRITLELRNRGHALNHKTVRRLMNILGLKCLVRLKKYRSYKGTVGKVAPNILKRNFHASKPNEKWV
TDVTEFHLHGKKLYLSPILDLYNGEIIAYNIEHRPAYSLVSKMLNKAFQRLNDKETPILHSDQGWHYQMRQYHQSLKKHNVIQSMSRKGNCLDNAVMENF
FGLLKSELLYLKEFESMEQFKQELETYIHYYNHKRIKTKLKGLSPVQYRVQSLVAA
Blast result :
Comments
ISBth167 is 75% aa similar to ISBce15.
References
1] ISfinder annotation (2009)
2] Huang,J., Guo,S., Mahillon,J., Van der Auwera,G.A., Wang,L., Han,D., Yu,Z. and Sun,M. (2006) BMC Genomics 7, 270
2] Huang,J., Guo,S., Mahillon,J., Van der Auwera,G.A., Wang,L., Han,D., Yu,Z. and Sun,M. (2006) BMC Genomics 7, 270