ISArsp3

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008541 ND Arthrobacter sp.
Arthrobacter sp. FB24
Arthrobacter sp. FB24 plasmid 2
DNA section
IS Length : 1596 bp

Ends


Left end : AAGTGAGTTTCTCAGGGGCGCTTTCGCTCCGCCGTGGGGGCCGAAAATGTACTGCTGTGGGGAACCTTTGCGGTGTCGGGGCTGCCGGAGCAGACTGAAG
Right end : TCGGCTATCAAGTCATTCTCGAACCCCTGCAACAGACCGGATAACACCGCTCACCCCACGCACCCCACCGCACTCACCTAATTTTCATGTCAGAGAGCGT

Insertion site


Left flankDirect repeatRight flankDR Length
GCCGCGGACCCAGGATTTAC0

DNA sequence

AAGTGAGTTTCTCAGGGGCGCTTTCGCTCCGCCGTGGGGGCCGAAAATGTACTGCTGTGGGGAACCTTTGCGGTGTCGGGGCTGCCGGAGCAGACTGAAG
AGTGTGTTCCGGTCGACCCCGGTCTCTCTTTAGTGCCGGTGAGCCTGTAAGTGAGTTTGGTGGTGGAGGGCGCAGCTTTTGTCCGCGGGACCGTGGAATG
TTGCCGTTGAGCACGAATGTCAGATTTGTTGTTTATCCGCCTTCCCCGGGACACAGACGCCATCTGTTGGACTGTTCGAGGAAGGGTGAGCTCTCATGGA
GGTCATTCACCCGCGCTGCGCGGGTGTCGATATTTCAAAGAAGGACGCGAAGGTCTGTGTCCGCATCCAGGGCCGCGGCGGCCGGTCCACCACATCCACC
ATCACGACCTGGGGTTCGATGACGGGGCAGATCCTGGGGCTAAAGGAACACCTCCTGGACGAGCACGTGGATCTGGTGGTCATGGAGGCCACCGGTGATT
ACTGGCGCCCCTTCTACTACCTGCTTGAAGACGACGGACTGAACATCATCCTGGTCAATGCCCATGACGCCAGGAACGTCCCGGGCCGCAAAACCGACGT
TTCCGACGCTGCCTGGCTTGCCGACCTGGGCGCCCACGGGCTGGTCCGGGCCTCCTTCGTGCCCCCGCCTCCGATCCGTGAACTGCGGGACCTGACCCGG
GCCCGGACCATCATCACTCAGGAACGGACCCGGGAAATCCAGCGCCTGGAGAAACTCCTCGAGGACGCCTGCATCAAACTATCCTCGGTAGCTTCGAACA
TCACCGGGGTCTCCGGACGACTGATCCTCCAAGCACTCATCGACGGGCAGACCGACCCCGCGGCCCTGGCAGAGATGGCCCAACGCCGGCTGCGGTCCAA
AATCCCTGAACTCACCCAAGCCCTCAACGGCCGGTTCACCGAACACCACCGCTACATGACCGGACTCTATCTGCACCGGATTGATGCCCACACCGCCGAT
ATCAACGACCTCAGCGCCAGGATCGAGGCGGCGATGGAGCCCTTTCGTTTCGCCCGGGAGCTCCTCGTGAGCATCCCGGGGTTCAGCACCACCATCGCGG
AAATCTTCATCGCCGAAACCGGCGCCGACATGAGCGCATTCGCCACCGCAGGGCAGCTGGCATCCTGGGCCGGTACTTCCCCGGGATCCAACGAATCCGC
CGGACGGGTCAAATCCACCAAAACACGGCCCGGCAACCGGTACCTCAAAGGTGCCCTGGGCATCGCTGCTCTGTCCTGCGCGAAATCGAAGAACACCTAC
CTCGGTGCCAGATACCGGCGCATTGCCTCCCGGCGCGGACCCGCCAAAGCCCTCGTCGCCGTCGAACACTCCATCCTCACCGCAGCGTGGCACATGCTCA
CCACCGGCGAACTCTACAACGACCCCGGCGCCGACTACTTCACCCGGCAGACACCCGTTAAAACCATGGCCCGGGCCGTCAGACAACTAGAATCCCTCGG
CTATCAAGTCATTCTCGAACCCCTGCAACAGACCGGATAACACCGCTCACCCCACGCACCCCACCGCACTCACCTAATTTTCATGTCAGAGAGCGT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1245 bp414 aa2961540+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MEVIHPRCAGVDISKKDAKVCVRIQGRGGRSTTSTITTWGSMTGQILGLKEHLLDEHVDLVVMEATGDYWRPFYYLLEDDGLNIILVNAHDARNVPGRKT
DVSDAAWLADLGAHGLVRASFVPPPPIRELRDLTRARTIITQERTREIQRLEKLLEDACIKLSSVASNITGVSGRLILQALIDGQTDPAALAEMAQRRLR
SKIPELTQALNGRFTEHHRYMTGLYLHRIDAHTADINDLSARIEAAMEPFRFARELLVSIPGFSTTIAEIFIAETGADMSAFATAGQLASWAGTSPGSNE
SAGRVKSTKTRPGNRYLKGALGIAALSCAKSKNTYLGARYRRIASRRGPAKALVAVEHSILTAAWHMLTTGELYNDPGADYFTRQTPVKTMARAVRQLES
LGYQVILEPLQQTG

 

Blast result :
Comments
ISArsp3 is 62% aa similar to ISAzo28.
References
1] ISfinder annotation (2009)

2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Thompson,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Beasley,F., Chen,W., Jerke,K., Nakatsu,C.H. and Richardson,P. (2006) US DOE Joint Genome Institute. Direct submission GenBank.