ISAcl2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011881 | ND | Arthrobacter chlorophenolicus | Arthrobacter chlorophenolicus A6 plasmid pACHL02 |
DNA section
IS Length : 1243 bp
Ends
IR Length : 32/42
IRL : GGGACTGCCCCCGGTTTTGTTGACTCCTTGACCTAGCGAGCTTGTGCTCG
IRR : GGGACTGCCCCGGCTTTTGTTGACTCGGGGTCTTAGGCCGCTGTGAGCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCTAAGTTG | CCC | ATCTATGGTT | 3 |
DNA sequence
GGGACTGCCCCCGGTTTTGTTGACTCCTTGACCTAGCGAGCTTGTGCTCGTGGAAGGATGTTGACCATGCCCACGGCTTATGGGGCGGAGTTCCGCCAGG
ATGTTATTGATGTGGCCCGCAAGGGCGAGGCGCCGCTGGCGCAGATTGCGAAGGATTTTGGGCTTTCGGTCACGACGTTGAAGCGCTGGATCGCTATCGC
TGAGCGTAAGGACTCCGGGACCAGCCCAGCGGCGGCGGAGTCGGCTGAGGTGCGGGAGTTGAAGAAGCGGAACCGCCTGTTGGAGCAGGAGAACGAGATT
CTGCGCCGGGCGGCTGCCTATCTGGCCAGGGACATCAACCCAAAATGATCTACCCGCTGGTCACGGATCTTGCCGCCGACGGTGTTCCCGTGGCGGTGAC
CTGCAGGGTGTTGGGATTTAGCAAGCAAGGCTATTACCGCTGGAAGGCGAACCCGGTGACGGAACGGGACTGGATCGATGCGCACCTGGTCAACGCCGCC
CTTGACATCCACTCCGACGATCCGGCGTTCGGGTACCGGTTCATCGCCGACGAGCTCCCGGAGAAGGGCATCACCGCGGGCGAGAACAGGGTCCAGCGCT
TATGCAGGGACCACGGCATCTGGTCAGTGTTCTCGAAGAAACGGGGTCTGAACCGGAAACCGGGGCCACCGGTCCACGACGACCTGGTCGAGCGGGATTT
CACCGCCGCGGCGCCGAACGAACTGTGGCTGACAGACATCACCGAGCACCCCACCGAAGAAGGGAAGCTCTACCTCTGCGCGGTGAAGGACGTCTACTCC
GGCAGGATCGTCGGCTACTCAATGGACGCCCGGATGAAGTCCTCTCTTGCCGTCGCTGCCCTGGAGAACGCGGTGCAGGCACGAAGACCGGAAAGAACGG
TGGTCCACTCCGACCGTGGGTCGCAGTTCCGGTCACGCCGGTTCGTTGAATCGCTCCGTCGCCACGGGCTCACCGGATCGATGGGCAGGGTCGGTGCGTG
CGCGGACAACGCCGCGATGGAGTCCTTCTTCTCGCTGCTGCAGAAGAACGTCCTGGACCGTCAGCGATGGCTCACCAGACAGGACCTCCGGCTGGCCATC
ACAACCTGGATCGAGAGAACCTACCACCGCCGGCGACGGCAAAGACGGCTGGGCAAACTCACGCCCATCGAATATGAAACAATCAACCGGACCGCGCTCA
CAGCGGCCTAAGACCCCGAGTCAACAAAAGCCGGGGCAGTCCC
ATGTTATTGATGTGGCCCGCAAGGGCGAGGCGCCGCTGGCGCAGATTGCGAAGGATTTTGGGCTTTCGGTCACGACGTTGAAGCGCTGGATCGCTATCGC
TGAGCGTAAGGACTCCGGGACCAGCCCAGCGGCGGCGGAGTCGGCTGAGGTGCGGGAGTTGAAGAAGCGGAACCGCCTGTTGGAGCAGGAGAACGAGATT
CTGCGCCGGGCGGCTGCCTATCTGGCCAGGGACATCAACCCAAAATGATCTACCCGCTGGTCACGGATCTTGCCGCCGACGGTGTTCCCGTGGCGGTGAC
CTGCAGGGTGTTGGGATTTAGCAAGCAAGGCTATTACCGCTGGAAGGCGAACCCGGTGACGGAACGGGACTGGATCGATGCGCACCTGGTCAACGCCGCC
CTTGACATCCACTCCGACGATCCGGCGTTCGGGTACCGGTTCATCGCCGACGAGCTCCCGGAGAAGGGCATCACCGCGGGCGAGAACAGGGTCCAGCGCT
TATGCAGGGACCACGGCATCTGGTCAGTGTTCTCGAAGAAACGGGGTCTGAACCGGAAACCGGGGCCACCGGTCCACGACGACCTGGTCGAGCGGGATTT
CACCGCCGCGGCGCCGAACGAACTGTGGCTGACAGACATCACCGAGCACCCCACCGAAGAAGGGAAGCTCTACCTCTGCGCGGTGAAGGACGTCTACTCC
GGCAGGATCGTCGGCTACTCAATGGACGCCCGGATGAAGTCCTCTCTTGCCGTCGCTGCCCTGGAGAACGCGGTGCAGGCACGAAGACCGGAAAGAACGG
TGGTCCACTCCGACCGTGGGTCGCAGTTCCGGTCACGCCGGTTCGTTGAATCGCTCCGTCGCCACGGGCTCACCGGATCGATGGGCAGGGTCGGTGCGTG
CGCGGACAACGCCGCGATGGAGTCCTTCTTCTCGCTGCTGCAGAAGAACGTCCTGGACCGTCAGCGATGGCTCACCAGACAGGACCTCCGGCTGGCCATC
ACAACCTGGATCGAGAGAACCTACCACCGCCGGCGACGGCAAAGACGGCTGGGCAAACTCACGCCCATCGAATATGAAACAATCAACCGGACCGCGCTCA
CAGCGGCCTAAGACCCCGAGTCAACAAAAGCCGGGGCAGTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 67 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MPTAYGAEFRQDVIDVARKGEAPLAQIAKDFGLSVTTLKRWIAIAERKDSGTSPAAAESAEVRELKKRNRLLEQENEILRRAAAYLARDINPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 249 | 1211 | + | No |
Description : Second part of the transposase
ORF sequence :
GAGVEEAEPPVGAGERDSAPGGCLSGQGHQPKMIYPLVTDLAADGVPVAVTCRVLGFSKQGYYRWKANPVTERDWIDAHLVNAALDIHSDDPAFGYRFIA
DELPEKGITAGENRVQRLCRDHGIWSVFSKKRGLNRKPGPPVHDDLVERDFTAAAPNELWLTDITEHPTEEGKLYLCAVKDVYSGRIVGYSMDARMKSSL
AVAALENAVQARRPERTVVHSDRGSQFRSRRFVESLRRHGLTGSMGRVGACADNAAMESFFSLLQKNVLDRQRWLTRQDLRLAITTWIERTYHRRRRQRR
LGKLTPIEYETINRTALTAA
DELPEKGITAGENRVQRLCRDHGIWSVFSKKRGLNRKPGPPVHDDLVERDFTAAAPNELWLTDITEHPTEEGKLYLCAVKDVYSGRIVGYSMDARMKSSL
AVAALENAVQARRPERTVVHSDRGSQFRSRRFVESLRRHGLTGSMGRVGACADNAAMESFFSLLQKNVLDRQRWLTRQDLRLAITTWIERTYHRRRRQRR
LGKLTPIEYETINRTALTAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 381 aa | 67 | 1211 | + | Yes |
Chemistry : DDE
ORF sequence :
MPTAYGAEFRQDVIDVARKGEAPLAQIAKDFGLSVTTLKRWIAIAERKDSGTSPAAAESAEVRELKKRNRLLEQENEILRRAAAYLARDINPKMIYPLVT
DLAADGVPVAVTCRVLGFSKQGYYRWKANPVTERDWIDAHLVNAALDIHSDDPAFGYRFIADELPEKGITAGENRVQRLCRDHGIWSVFSKKRGLNRKPG
PPVHDDLVERDFTAAAPNELWLTDITEHPTEEGKLYLCAVKDVYSGRIVGYSMDARMKSSLAVAALENAVQARRPERTVVHSDRGSQFRSRRFVESLRRH
GLTGSMGRVGACADNAAMESFFSLLQKNVLDRQRWLTRQDLRLAITTWIERTYHRRRRQRRLGKLTPIEYETINRTALTAA
DLAADGVPVAVTCRVLGFSKQGYYRWKANPVTERDWIDAHLVNAALDIHSDDPAFGYRFIADELPEKGITAGENRVQRLCRDHGIWSVFSKKRGLNRKPG
PPVHDDLVERDFTAAAPNELWLTDITEHPTEEGKLYLCAVKDVYSGRIVGYSMDARMKSSLAVAALENAVQARRPERTVVHSDRGSQFRSRRFVESLRRH
GLTGSMGRVGACADNAAMESFFSLLQKNVLDRQRWLTRQDLRLAITTWIERTYHRRRRQRRLGKLTPIEYETINRTALTAA
Blast result :
Comments
ISAcl2 is 82% aa similar to ISMsm7.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The extremities are not the typical IRs of the family (TGa/g)
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The extremities are not the typical IRs of the family (TGa/g)
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Goltsman,E., Clum,A., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Jansson,J. and Richardson,P. (2009) Direct Submission to GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Goltsman,E., Clum,A., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Jansson,J. and Richardson,P. (2009) Direct Submission to GenBank.