ISAau2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008712 ND Arthrobacter aurescens
Arthrobacter aurescens TC1 plasmid TC1
DNA section
IS Length : 2222 bp

Ends


IR Length : 36/50

IRL : TGTCAGCGACGGCTGAAAACTGACCCCGGAGCGTCGGCCGAATTTTGACC
IRR : TGTCAACGCCGCCCGAAAATTGACCCCTTTTCGCCGGTTGAAAATTGACC

Insertion site


Left flankDirect repeatRight flankDR Length
AAACGCTCGGTCGCTCAGCCGCCGT5

DNA sequence

TGTCAGCGACGGCTGAAAACTGACCCCGGAGCGTCGGCCGAATTTTGACCCCCTTCGCACTGACTCTCGAGCTCAAGCGAGCTCGAGAGGAGAAGGGAGT
TGTTGTCTGTGGAGGACTGGGCTGAGATCCGCCGGCTGCGTCGAGCGGAGGGGTTGGGCATCAAGACGATCGCCAAGACGTTGGGGATCTCGCGGAACAC
GGTGCGGGCCGCGATCGCTTCGGACGCGCCGCCGAAGTATCGGCGGCGGCCGGCGGGGTCGGCGGTGGATGCGTTCGAGGACGCGATCCGCCAGCAGCTT
GCGGCGGTGCCGACGATGCCGGCGACGGTGATCGCCGAGCGCGTGGGGTGGACGCGTGGGATCACGGTGTTCAAGGAGCGGGTCGCTGAGCTGCGGCCGG
CCTACCTGCCGCCGGACCCGGCGGGCCGGACGACCTATGTGGCCGGGGAGATCGCCCAGTGCGACCTGTGGTTCCCACCGATCCAGCTGCCGGTCGGGTT
CGGGCAGACCCGTGGCCCCAAGCAGCTGCCGGTGTTGACGATGGTGACCGGCTACTCCCGCTGGCTGTCGGGGATCTTGATCCCGTCGCGGCGGGCCGAG
GACCTGTTCGCCGGCTGGTGGCAGCTGATCTCCGCGCTCGGGGCGGTGCCGCGGCTGTTGGTCTGGGACGGCGAGGGCGCGATTGGCCGCAACCGTGGCG
GCCGGATCGAGCTCACCGGCGATTGCCAGGCGTTCCGCGGGGTGCTGGGGACGAAGGTGATCGTGCTCAAGCCGGCCGAGCCGGAGCACAAGGGCATCAT
CGAACGTGCCCATGACTACCTGGAGCGCTCGTTCCTGCCCGGGCGGGTGTTCAGCGACCCGGCCGACTTCAACGTCCAGCTGCAGGGCTGGCTGACCCAG
GTCAACGCCCGCACCCGCCGCGCGTTGGGCTGCGCTCCGACCGACCGGATCGCAGCCGATCGGCAAGCGATGCTCACCCTGCCACCGGTCGCCCCGGCGG
TCGGCTGGCGGCACTCGACGAGGCTGGCCCGGGACCACTACGTGCGGCTGGACTCCAACGACTACTCCGTCCACCCCGCGGTGATCGGCCGCCGCATCGA
GGTGGTCGCCGACCTGGACCGGGTCAAGGTGTTCTGCGAAGGCCGCGTGGTGGCCGACCACCAGCGGGTCTGGGCCTGGCACCAGACGATCACCGACCCC
GAGCACCTGCAGGCCGCCAAGACGCTGCGGCGGACCCGCGTCGGGGCGTTGCGGCCGGTCCGCGACGCCGAGACCGAGCTCGCCGTCGAACAACGCCCGC
TGGGCGACTACGACACCGCCCTCGGCATCGACCTCGGCGGTGAAGGCGGGTTCGCATCATGACCGCCCGAAAGACCGCGGCTGCTGCGCAGCGCGACCTG
GCCAGTGAGGTGAGCTTCCTGACCCGGGCGTTGAAGGCGCCGACGCTGCGGGAGGCGGTGCCGCGGTTGGCTGAGCGGGCTCGGGCGGAGTCCTGGACCC
ATGAGGAGTTCCTGGTCGCCTGCCTGCAACGCGAGGTCTCCGCCCGGGAGTCCCACGGCGGTGAGGGCCGGATCCGGTCCGCCCGGTTCCCCGCGCGGAA
GAGCCTGGAGGAGTTCGACTTCGACCACGCCCGCGGGCTGAAACGCGACCTGATCGCCCACCTAGGCACCCTCGACTTCGTCGTCGCACGCGAGAACGTG
GTCTTCCTCGGCCCACCCGGCACCGGGAAGACCCACCTCGCCATCGGCCTGGCGATCAGGGCCTGCCAGGCCGGGCACCGGGTGCTGTTCGCCACCGCAA
GTGAGTGGGTGGCCCGGCTCGCCGAGGCCCACCACGCCGGCCGGCTCCACGACGAGCTGGTGCGGCTCGGCCGCTACCCGCTGCTGGTGGTCGACGAGGT
CGGCTACATCCCCTTCGAGCCCGAGGCCGCCAACCTGTTCTTCCAGCTCGTCTCCAGCCGCTACGAGCGCGCCTCGCTGATCGTCACCTCCAACAAGCCC
TTCGGCCGCTGGGGCGAGGTCTTCGGCGACGACGTCGTCGCCGCCGCGATGATCGACCGGCTCGTCCACCACGCCGAGGTCGTTGCCCTCAAGGGCGACT
CCTACCGGCTCAAAGACCGCGACCTCGGCCGAGTGCCGGCGTCGACAACCGAAGAACCATGACCACCAAGGGGGTCAATTTTCAACCGGCGAAAAGGGGT
CAATTTTCGGGCGGCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1263 bp420 aa1001362+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LLSVEDWAEIRRLRRAEGLGIKTIAKTLGISRNTVRAAIASDAPPKYRRRPAGSAVDAFEDAIRQQLAAVPTMPATVIAERVGWTRGITVFKERVAELRP
AYLPPDPAGRTTYVAGEIAQCDLWFPPIQLPVGFGQTRGPKQLPVLTMVTGYSRWLSGILIPSRRAEDLFAGWWQLISALGAVPRLLVWDGEGAIGRNRG
GRIELTGDCQAFRGVLGTKVIVLKPAEPEHKGIIERAHDYLERSFLPGRVFSDPADFNVQLQGWLTQVNARTRRALGCAPTDRIAADRQAMLTLPPVAPA
VGWRHSTRLARDHYVRLDSNDYSVHPAVIGRRIEVVADLDRVKVFCEGRVVADHQRVWAWHQTITDPEHLQAAKTLRRTRVGALRPVRDAETELAVEQRP
LGDYDTALGIDLGGEGGFAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp267 aa13592162+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTARKTAAAAQRDLASEVSFLTRALKAPTLREAVPRLAERARAESWTHEEFLVACLQREVSARESHGGEGRIRSARFPARKSLEEFDFDHARGLKRDLIA
HLGTLDFVVARENVVFLGPPGTGKTHLAIGLAIRACQAGHRVLFATASEWVARLAEAHHAGRLHDELVRLGRYPLLVVDEVGYIPFEPEAANLFFQLVSS
RYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPASTTEEP

 

Blast result :
Comments
ISAau2 is 79% (istA, the transposase) and 94% (istB, the helper of transposition) aa similar to ISMt3.
References
1] ISfinder annotation (2009)
2] Mongodin,E.F., Shapir,N., Daugherty,S.C., DeBoy,R.T., Emerson,J.B., Shvartzbeyn,A., Radune,D., Vamathevan,J., Riggs,F., Grinberg,V., Khouri,H., Wackett,L.P., Nelson,K.E. and Sadowsky,M.J. (2006) PLoS Genet. 2 (12), E214