ISAar46
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1526 bp
Ends
IR Length : 15/19
IRL : TGGAACGGTACCAACGCCACACAGCAAATCCACTCCAGCACGTAGGGCGA
IRR : TCAAACGGTACCAACACGAGTCTAAATAACTATCAAGCAGCCTGAACTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTGTCAGGC | CTTGAAAAAT | 0 |
DNA sequence
TGGAACGGTACCAACGCCACACAGCAAATCCACTCCAGCACGTAGGGCGAGAACCAGGAAATCATCCTCAATGCCTGACCTAGCCACAACCACCATAGGA
CAAGATAGTCCTATGACTAATCCCGGACCCCGCGCCGGTGGACCGTCCCGACGCAGGACGTTCACCCCAACCGAGAAACTTGAACTGCTTGCCGGTTACG
ACGAAGCTATCACCGAGCAGAAGGGCGGCGCCTACCTGCGTGAGCATGGCGTGTATTCCTCGCATATCACCGAATGGCGCAAACTACGCGATGCCGGCGT
CCTCGAAGGAAAAACAGCCGGAACCCGTCTCGGCAAGCTCAGCGCCGAACAGGCCGAGATTGCCCGCCTACGCCGCCAGTTAGAGGTGAGTGAGGGCAAG
CGGAAACAGACGGAAGAAGCCTTAGATATCATGGGAAAACTCTCGGCGTTCTTCGAGAACGCCATCAACGAGTCGCAGGACGAGGCGAAGTCCAAGAAGA
AATGAAAGCCGCGTACCAGCGGTTGCTTGACATCAAGATCCCGCAACGGAAAGCCGCTGTCATAGCGGGCGTTTCACGCACCACAATGAACCGCAAACCG
AGCAAGCCGCAGGATACTCCGCGTCCGGCGCCACCGAACAAGCTCAGCCATGTTGAACGGGCTGCGATTCTAAGTGCTTTGAATAGCCCTGAGTGGGTGG
ATATGGCCCCGATGCAGGTCTATGCGAAGCTACTCGATGAAGGTCAATATTTGGGTTCTCTTTCATCGTTTTATCGGGTGCTCAATGAGAACCGGCTGGT
CAAGGAACGCCGCCGTTTAGCGAGGCATAAGCCGCGTGCGATCCCAGAGCTGGAAGCTAATGCACCAGGTGAAGTCTATAGTTGGGACATCACAAAACTG
GCTGGTCCGGTGAAGGGTAAGTACTTTGACTGCTATTTGATGGTCGATATTTATTCGCGGTATATCGTTGGCGCGCATGTGCACGCAACCGAGTCCGGCC
AGCTAGCCGTGGAGATGATGAAAGAGATCTTTGGCGTCCATGGAATCCCCCTGGTTGTTCACGCGGACCGCGGAACGAGTATGACGTCGAAGACGGTCGC
GGCTTTGCTCTCTGATTTGGAAGTGACTAGGTCGCATTCGCGGCCTCGGGTCAGCAACGACAACCCCTACAGCGAGTCGTTGTTCAAGACTTTGAAGTAT
GCTCCGCAGTTCCCTGAACGTTTCTCTTCATTGAGTGATGCGCGCTTATTTATCAGTGAATTCGTGCAGTGGTACAACCATGCTCATCAGCATTCGGGGA
TCGGGTTGCATACGCCGGCGGACGTTCATTTTGGGTTGGCTAATTCGGTCGCTGCGAAACGTTCACAAACGCTGGCAGCGGCTCGTGCGCGGCATCCTGA
ACGCTTTAGTACGTCGCAGGATCCGAAGATTTTGGCGATGCCTGAAGCTGCATGGATTAACAATCCGAAGGATCGGGCAGTTCAGGCTGCTTGATAGTTA
TTTAGACTCGTGTTGGTACCGTTTGA
CAAGATAGTCCTATGACTAATCCCGGACCCCGCGCCGGTGGACCGTCCCGACGCAGGACGTTCACCCCAACCGAGAAACTTGAACTGCTTGCCGGTTACG
ACGAAGCTATCACCGAGCAGAAGGGCGGCGCCTACCTGCGTGAGCATGGCGTGTATTCCTCGCATATCACCGAATGGCGCAAACTACGCGATGCCGGCGT
CCTCGAAGGAAAAACAGCCGGAACCCGTCTCGGCAAGCTCAGCGCCGAACAGGCCGAGATTGCCCGCCTACGCCGCCAGTTAGAGGTGAGTGAGGGCAAG
CGGAAACAGACGGAAGAAGCCTTAGATATCATGGGAAAACTCTCGGCGTTCTTCGAGAACGCCATCAACGAGTCGCAGGACGAGGCGAAGTCCAAGAAGA
AATGAAAGCCGCGTACCAGCGGTTGCTTGACATCAAGATCCCGCAACGGAAAGCCGCTGTCATAGCGGGCGTTTCACGCACCACAATGAACCGCAAACCG
AGCAAGCCGCAGGATACTCCGCGTCCGGCGCCACCGAACAAGCTCAGCCATGTTGAACGGGCTGCGATTCTAAGTGCTTTGAATAGCCCTGAGTGGGTGG
ATATGGCCCCGATGCAGGTCTATGCGAAGCTACTCGATGAAGGTCAATATTTGGGTTCTCTTTCATCGTTTTATCGGGTGCTCAATGAGAACCGGCTGGT
CAAGGAACGCCGCCGTTTAGCGAGGCATAAGCCGCGTGCGATCCCAGAGCTGGAAGCTAATGCACCAGGTGAAGTCTATAGTTGGGACATCACAAAACTG
GCTGGTCCGGTGAAGGGTAAGTACTTTGACTGCTATTTGATGGTCGATATTTATTCGCGGTATATCGTTGGCGCGCATGTGCACGCAACCGAGTCCGGCC
AGCTAGCCGTGGAGATGATGAAAGAGATCTTTGGCGTCCATGGAATCCCCCTGGTTGTTCACGCGGACCGCGGAACGAGTATGACGTCGAAGACGGTCGC
GGCTTTGCTCTCTGATTTGGAAGTGACTAGGTCGCATTCGCGGCCTCGGGTCAGCAACGACAACCCCTACAGCGAGTCGTTGTTCAAGACTTTGAAGTAT
GCTCCGCAGTTCCCTGAACGTTTCTCTTCATTGAGTGATGCGCGCTTATTTATCAGTGAATTCGTGCAGTGGTACAACCATGCTCATCAGCATTCGGGGA
TCGGGTTGCATACGCCGGCGGACGTTCATTTTGGGTTGGCTAATTCGGTCGCTGCGAAACGTTCACAAACGCTGGCAGCGGCTCGTGCGCGGCATCCTGA
ACGCTTTAGTACGTCGCAGGATCCGAAGATTTTGGCGATGCCTGAAGCTGCATGGATTAACAATCCGAAGGATCGGGCAGTTCAGGCTGCTTGATAGTTA
TTTAGACTCGTGTTGGTACCGTTTGA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 393 bp | 130 aa | 113 | 505 | + | No |
Description : First part of the transposase
ORF sequence :
MTNPGPRAGGPSRRRTFTPTEKLELLAGYDEAITEQKGGAYLREHGVYSSHITEWRKLRDAGVLEGKTAGTRLGKLSAEQAEIARLRRQLEVSEGKRKQT
EEALDIMGKLSAFFENAINESQDEAKSKKK
EEALDIMGKLSAFFENAINESQDEAKSKKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1101 bp | 366 aa | 394 | 1494 | + | No |
Description : Second part of the transposase
ORF sequence :
GQAETDGRSLRYHGKTLGVLRERHQRVAGRGEVQEEMKAAYQRLLDIKIPQRKAAVIAGVSRTTMNRKPSKPQDTPRPAPPNKLSHVERAAILSALNSPE
WVDMAPMQVYAKLLDEGQYLGSLSSFYRVLNENRLVKERRRLARHKPRAIPELEANAPGEVYSWDITKLAGPVKGKYFDCYLMVDIYSRYIVGAHVHATE
SGQLAVEMMKEIFGVHGIPLVVHADRGTSMTSKTVAALLSDLEVTRSHSRPRVSNDNPYSESLFKTLKYAPQFPERFSSLSDARLFISEFVQWYNHAHQH
SGIGLHTPADVHFGLANSVAAKRSQTLAAARARHPERFSTSQDPKILAMPEAAWINNPKDRAVQAA
WVDMAPMQVYAKLLDEGQYLGSLSSFYRVLNENRLVKERRRLARHKPRAIPELEANAPGEVYSWDITKLAGPVKGKYFDCYLMVDIYSRYIVGAHVHATE
SGQLAVEMMKEIFGVHGIPLVVHADRGTSMTSKTVAALLSDLEVTRSHSRPRVSNDNPYSESLFKTLKYAPQFPERFSSLSDARLFISEFVQWYNHAHQH
SGIGLHTPADVHFGLANSVAAKRSQTLAAARARHPERFSTSQDPKILAMPEAAWINNPKDRAVQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1382 bp | 460 aa | 113 | 1494 | + | Yes |
Chemistry : DDE
ORF sequence :
MTNPGPRAGGPSRRRTFTPTEKLELLAGYDEAITEQKGGAYLREHGVYSSHITEWRKLRDAGVLEGKTAGTRLGKLSAEQAEIARLRRQLEVSEGKRKQT
EEALDIMGKTLGVLRERHQRVAGRGEVQEEMKAAYQRLLDIKIPQRKAAVIAGVSRTTMNRKPSKPQDTPRPAPPNKLSHVERAAILSALNSPEWVDMAP
MQVYAKLLDEGQYLGSLSSFYRVLNENRLVKERRRLARHKPRAIPELEANAPGEVYSWDITKLAGPVKGKYFDCYLMVDIYSRYIVGAHVHATESGQLAV
EMMKEIFGVHGIPLVVHADRGTSMTSKTVAALLSDLEVTRSHSRPRVSNDNPYSESLFKTLKYAPQFPERFSSLSDARLFISEFVQWYNHAHQHSGIGLH
TPADVHFGLANSVAAKRSQTLAAARARHPERFSTSQDPKILAMPEAAWINNPKDRAVQAA
EEALDIMGKTLGVLRERHQRVAGRGEVQEEMKAAYQRLLDIKIPQRKAAVIAGVSRTTMNRKPSKPQDTPRPAPPNKLSHVERAAILSALNSPEWVDMAP
MQVYAKLLDEGQYLGSLSSFYRVLNENRLVKERRRLARHKPRAIPELEANAPGEVYSWDITKLAGPVKGKYFDCYLMVDIYSRYIVGAHVHATESGQLAV
EMMKEIFGVHGIPLVVHADRGTSMTSKTVAALLSDLEVTRSHSRPRVSNDNPYSESLFKTLKYAPQFPERFSSLSDARLFISEFVQWYNHAHQHSGIGLH
TPADVHFGLANSVAAKRSQTLAAARARHPERFSTSQDPKILAMPEAAWINNPKDRAVQAA
Blast result :
Comments
ISAar46 is 67% aa similar to ISAar44.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).