ISAar45
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1280 bp
Ends
IR Length : 26
IRL : GGGTCTGCTGAAGGTTTGGTTGACACTTCGACCGCATAACCCAGCTATGG
IRR : GGGTCTGCTGAAGGTTTGGTTGACACCAAGGTTTACGCTGCCAGAGCGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCACGTTG | GAT | GCTTAGACGC | 3 |
DNA sequence
GGGTCTGCTGAAGGTTTGGTTGACACTTCGACCGCATAACCCAGCTATGGTTATGCTCAAACGAAGGAACAAAACCATGCCAAGAATCTTCACCGAAGAA
TTCAAGAAGGACGCCGTCGCTCTCGTCGAATCGGGGATATCCCAGAAGCAAGTCTCCGCGGATCTGGGAGTCTCAAAGACAGCCCTGCAAACCTGGGTCC
GCGATGCCCGCTACCAATCCCACGGAATGACACCGTCCTCCGATGCTGACGAACGCAAGGAAATGTCCCAAGCGCTCAAACGCATCCGGGAGCTCGAAAT
GGAGAACGAGGTGCTGCGCAAGGCCGCAGCCTACCTTTCCCAGTCGCATATTCGTTCCCCAAAATAATGGGCCGGCTCGTCCAAGAATTAGCCGCCAGAA
CAGGTAGGAAACATATTCCTGTGGCGGTGACGTGTCGGGTCTTGGGATTCAGCACCCAGGCGTACTACAAGTGGTTGAAGAGCCCTGTCTCAGCCCGGGA
AATCGAAGAGCGGGAGTTGATCGGCAAACTGCATGAGCTTCATGAGGAAGATCCCGAGGGCGGGTACCGGGTGTTGGCCGATGATCTGGAAGACGCCGGA
TACCAGGTCTCCGAGCGCCGGGTCTGGCGGTTATGCCGCAAGGCAGGGATCCGCTCAGTGATCAGCAAACGCCAGCGCAAGCACGTTCCGGCGGGCCCTG
CGGTGCATGACGACTTGGTGCAGCGGAACTTCACCGCTGACCAGCCTAACCAGTTGTGGCTGACCGATATTACAGAGCACCACACGAGCGAAGGAAAGCT
GTATTTGTGCGCGATTAAGGACGTGTTCAGCAACAGAATCGTGGGGTACTCGATTGATTCGAGGATGAAGGCCCACCTGGCCGTCAACGCCGTGGAAATG
GCTGTGGCGCACCGTGGCCACCCGCGCGGGGTCAAGGTCCACAGCGACCGTGGCAGCCAGTTCCGGTCTAAGAAATTCACGCAGGCTCTCGAACGTCACG
GGTTGGTCGGTTCGATGGGCAGGGTCGGGGCAGCCGGCGACAACGCGGCCATGGAGTCGTTTTTCAGTCTGTTGCAGAAGAATGTGCTGGACCGCCGTTG
GTGGTCCTCCCGCCAACAACTTCGTCTGGCCATTATTGGATGGCTGGAGGGGAAGTATCATCGCAAGCGTAAGCAAAGAGGTTTGGGGAGAATGACCCCG
ATCCAATTCGAGGCTACAATGATCGAGTCTGTCGCTCTGGCAGCGTAAACCTTGGTGTCAACCAAACCTTCAGCAGACCC
TTCAAGAAGGACGCCGTCGCTCTCGTCGAATCGGGGATATCCCAGAAGCAAGTCTCCGCGGATCTGGGAGTCTCAAAGACAGCCCTGCAAACCTGGGTCC
GCGATGCCCGCTACCAATCCCACGGAATGACACCGTCCTCCGATGCTGACGAACGCAAGGAAATGTCCCAAGCGCTCAAACGCATCCGGGAGCTCGAAAT
GGAGAACGAGGTGCTGCGCAAGGCCGCAGCCTACCTTTCCCAGTCGCATATTCGTTCCCCAAAATAATGGGCCGGCTCGTCCAAGAATTAGCCGCCAGAA
CAGGTAGGAAACATATTCCTGTGGCGGTGACGTGTCGGGTCTTGGGATTCAGCACCCAGGCGTACTACAAGTGGTTGAAGAGCCCTGTCTCAGCCCGGGA
AATCGAAGAGCGGGAGTTGATCGGCAAACTGCATGAGCTTCATGAGGAAGATCCCGAGGGCGGGTACCGGGTGTTGGCCGATGATCTGGAAGACGCCGGA
TACCAGGTCTCCGAGCGCCGGGTCTGGCGGTTATGCCGCAAGGCAGGGATCCGCTCAGTGATCAGCAAACGCCAGCGCAAGCACGTTCCGGCGGGCCCTG
CGGTGCATGACGACTTGGTGCAGCGGAACTTCACCGCTGACCAGCCTAACCAGTTGTGGCTGACCGATATTACAGAGCACCACACGAGCGAAGGAAAGCT
GTATTTGTGCGCGATTAAGGACGTGTTCAGCAACAGAATCGTGGGGTACTCGATTGATTCGAGGATGAAGGCCCACCTGGCCGTCAACGCCGTGGAAATG
GCTGTGGCGCACCGTGGCCACCCGCGCGGGGTCAAGGTCCACAGCGACCGTGGCAGCCAGTTCCGGTCTAAGAAATTCACGCAGGCTCTCGAACGTCACG
GGTTGGTCGGTTCGATGGGCAGGGTCGGGGCAGCCGGCGACAACGCGGCCATGGAGTCGTTTTTCAGTCTGTTGCAGAAGAATGTGCTGGACCGCCGTTG
GTGGTCCTCCCGCCAACAACTTCGTCTGGCCATTATTGGATGGCTGGAGGGGAAGTATCATCGCAAGCGTAAGCAAAGAGGTTTGGGGAGAATGACCCCG
ATCCAATTCGAGGCTACAATGATCGAGTCTGTCGCTCTGGCAGCGTAAACCTTGGTGTCAACCAAACCTTCAGCAGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 77 | 367 | + | No |
Description : First part of the transposase
ORF sequence :
MPRIFTEEFKKDAVALVESGISQKQVSADLGVSKTALQTWVRDARYQSHGMTPSSDADERKEMSQALKRIRELEMENEVLRKAAAYLSQSHIRSPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 999 bp | 332 aa | 250 | 1248 | + | No |
Description : Second part of the transposase
ORF sequence :
RTQGNVPSAQTHPGARNGERGAAQGRSLPFPVAYSFPKIMGRLVQELAARTGRKHIPVAVTCRVLGFSTQAYYKWLKSPVSAREIEERELIGKLHELHEE
DPEGGYRVLADDLEDAGYQVSERRVWRLCRKAGIRSVISKRQRKHVPAGPAVHDDLVQRNFTADQPNQLWLTDITEHHTSEGKLYLCAIKDVFSNRIVGY
SIDSRMKAHLAVNAVEMAVAHRGHPRGVKVHSDRGSQFRSKKFTQALERHGLVGSMGRVGAAGDNAAMESFFSLLQKNVLDRRWWSSRQQLRLAIIGWLE
GKYHRKRKQRGLGRMTPIQFEATMIESVALAA
DPEGGYRVLADDLEDAGYQVSERRVWRLCRKAGIRSVISKRQRKHVPAGPAVHDDLVQRNFTADQPNQLWLTDITEHHTSEGKLYLCAIKDVFSNRIVGY
SIDSRMKAHLAVNAVEMAVAHRGHPRGVKVHSDRGSQFRSKKFTQALERHGLVGSMGRVGAAGDNAAMESFFSLLQKNVLDRRWWSSRQQLRLAIIGWLE
GKYHRKRKQRGLGRMTPIQFEATMIESVALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 77 | 1248 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRIFTEEFKKDAVALVESGISQKQVSADLGVSKTALQTWVRDARYQSHGMTPSSDADERKEMSQALKRIRELEMENEVLRKAAAYLSQSHIRSPKIMGR
LVQELAARTGRKHIPVAVTCRVLGFSTQAYYKWLKSPVSAREIEERELIGKLHELHEEDPEGGYRVLADDLEDAGYQVSERRVWRLCRKAGIRSVISKRQ
RKHVPAGPAVHDDLVQRNFTADQPNQLWLTDITEHHTSEGKLYLCAIKDVFSNRIVGYSIDSRMKAHLAVNAVEMAVAHRGHPRGVKVHSDRGSQFRSKK
FTQALERHGLVGSMGRVGAAGDNAAMESFFSLLQKNVLDRRWWSSRQQLRLAIIGWLEGKYHRKRKQRGLGRMTPIQFEATMIESVALAA
LVQELAARTGRKHIPVAVTCRVLGFSTQAYYKWLKSPVSAREIEERELIGKLHELHEEDPEGGYRVLADDLEDAGYQVSERRVWRLCRKAGIRSVISKRQ
RKHVPAGPAVHDDLVQRNFTADQPNQLWLTDITEHHTSEGKLYLCAIKDVFSNRIVGYSIDSRMKAHLAVNAVEMAVAHRGHPRGVKVHSDRGSQFRSKK
FTQALERHGLVGSMGRVGAAGDNAAMESFFSLLQKNVLDRRWWSSRQQLRLAIIGWLEGKYHRKRKQRGLGRMTPIQFEATMIESVALAA
Blast result :
Comments
ISAar45 is 69% aa similar to ISMsm7.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).