ISAar43
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1247 bp
Ends
IR Length : 34/44
IRL : TGGGCTGACCCCGTTTGGTAGGTCCGGTGGTTGTGTGAGTCGTCCTGTTG
IRR : TGGGCCGCCCCCTTCATTCGGTCCGGTCGCTGTGCGAGTCGTCGGTTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGAGCATAA | GCCTCCCCCG | 0 |
DNA sequence
TGGGCTGACCCCGTTTGGTAGGTCCGGTGGTTGTGTGAGTCGTCCTGTTGCCCGGGGGTCCGGGCAGTGAGACTGGTCAGATCATGACGAACAGCAGGAA
GCGTCACACCTCGGAGCAGGTCGTCCGTAAGCTCGGGCAGGCCGACAGGATGCTCGCGGGCGGTAGCGACATCGCCGGGGTGTGTCGAGAGCTCGGAGTG
TCCGAGCAGACGTACTACCGGTGGCGGAACCAGTACGGCGGGCTGAAGGCCGACGACGCGAAGCGGCTGAAGGAGCTCGAGAAGCAGAACGCCACGCTCA
AGCGGCTGCTCGCCGAGGCAGAGCTCGAGAAGGCCGCGCTCAAGGAGCTGGCTGAGGGAAACTTCTAGGCCCGGGCAGGCGCCGCGCCGCCGTCGACCAC
CTCAAGCGCAAGCTGCGGGTGAGCGAACGAATGGCGTGCCGTCTGGCCGGGCTGAGCAGGTCCGCATACCGTCGCCCGCTCCAGGGTGAGACGACAGCCG
ACCCGGACCTGGCGTTGCGGGACTGGCTGCGGGCGTATGCGAAGAAGCACCCGCGGTGGGGATACCGCAGGGCCTACCACGATGCCCGCGGCGAGGGGTG
GGTCGTGAACCACAAGAAGATCCAACGGCTCTGGCGCGAGGAAGGGCTGCGCGTCCCGCAGCGGCGTCGCCGCAAACGCGTTGGGTCTTCGACCGTCGAC
GCCCCGGCAGCGGTCGCACCGAATCTCGTGTGGGCGGTGGACTTCCAGTTCGACGCGGACGAGCAGGGCCGTCCGATCAAGATCTGCTCCATCGTCGACG
AGCACACCCGCGAGTGCATCGGCGGGCTCGTCGAACGGTCGATCACCGCGGACCGGCTCACCGCCCACCTCGAGGACCTCGTCGCCGTCCGCGGCGCCCC
CGCGGTGCTCCGATCCGACAACGGCCCCGAGTTCATCAGCGACGCGATGGCCGACTGGGCCGGCACCCGCACCGGCCTGTTCTACATCCCGCCCGGCTCG
CCCTGGCACAACGGGTACGTCGAGTCGTTCAACAGCAGGCTCCGCGACGAGTGCCTGAACATCAACAGCTTCTACTCGCTGCTCCACGCCCAGGTCGTGA
TCGGCGACTGGAAGACCGAATACAACCACGACCGCCGGCATTCATCGCTCGGCTACCTCGCACCCGTCGACTACGCTCGGCAATGCACCCATCAATCGGA
AACCGACGACTCGCACAGCGACCGGACCGAATGAAGGGGGCGGCCCA
GCGTCACACCTCGGAGCAGGTCGTCCGTAAGCTCGGGCAGGCCGACAGGATGCTCGCGGGCGGTAGCGACATCGCCGGGGTGTGTCGAGAGCTCGGAGTG
TCCGAGCAGACGTACTACCGGTGGCGGAACCAGTACGGCGGGCTGAAGGCCGACGACGCGAAGCGGCTGAAGGAGCTCGAGAAGCAGAACGCCACGCTCA
AGCGGCTGCTCGCCGAGGCAGAGCTCGAGAAGGCCGCGCTCAAGGAGCTGGCTGAGGGAAACTTCTAGGCCCGGGCAGGCGCCGCGCCGCCGTCGACCAC
CTCAAGCGCAAGCTGCGGGTGAGCGAACGAATGGCGTGCCGTCTGGCCGGGCTGAGCAGGTCCGCATACCGTCGCCCGCTCCAGGGTGAGACGACAGCCG
ACCCGGACCTGGCGTTGCGGGACTGGCTGCGGGCGTATGCGAAGAAGCACCCGCGGTGGGGATACCGCAGGGCCTACCACGATGCCCGCGGCGAGGGGTG
GGTCGTGAACCACAAGAAGATCCAACGGCTCTGGCGCGAGGAAGGGCTGCGCGTCCCGCAGCGGCGTCGCCGCAAACGCGTTGGGTCTTCGACCGTCGAC
GCCCCGGCAGCGGTCGCACCGAATCTCGTGTGGGCGGTGGACTTCCAGTTCGACGCGGACGAGCAGGGCCGTCCGATCAAGATCTGCTCCATCGTCGACG
AGCACACCCGCGAGTGCATCGGCGGGCTCGTCGAACGGTCGATCACCGCGGACCGGCTCACCGCCCACCTCGAGGACCTCGTCGCCGTCCGCGGCGCCCC
CGCGGTGCTCCGATCCGACAACGGCCCCGAGTTCATCAGCGACGCGATGGCCGACTGGGCCGGCACCCGCACCGGCCTGTTCTACATCCCGCCCGGCTCG
CCCTGGCACAACGGGTACGTCGAGTCGTTCAACAGCAGGCTCCGCGACGAGTGCCTGAACATCAACAGCTTCTACTCGCTGCTCCACGCCCAGGTCGTGA
TCGGCGACTGGAAGACCGAATACAACCACGACCGCCGGCATTCATCGCTCGGCTACCTCGCACCCGTCGACTACGCTCGGCAATGCACCCATCAATCGGA
AACCGACGACTCGCACAGCGACCGGACCGAATGAAGGGGGCGGCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 84 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTNSRKRHTSEQVVRKLGQADRMLAGGSDIAGVCRELGVSEQTYYRWRNQYGGLKADDAKRLKELEKQNATLKRLLAEAELEKAALKELAEGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 356 | 1234 | + | No |
Description : Second part of the transposase
ORF sequence :
GKLLGPGRRRAAVDHLKRKLRVSERMACRLAGLSRSAYRRPLQGETTADPDLALRDWLRAYAKKHPRWGYRRAYHDARGEGWVVNHKKIQRLWREEGLRV
PQRRRRKRVGSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMAD
WAGTRTGLFYIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE
PQRRRRKRVGSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMAD
WAGTRTGLFYIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 84 | 1234 | + | Yes |
Chemistry : DDE
ORF sequence :
MTNSRKRHTSEQVVRKLGQADRMLAGGSDIAGVCRELGVSEQTYYRWRNQYGGLKADDAKRLKELEKQNATLKRLLAEAELEKAALKELAEGKLLGPGRR
RAAVDHLKRKLRVSERMACRLAGLSRSAYRRPLQGETTADPDLALRDWLRAYAKKHPRWGYRRAYHDARGEGWVVNHKKIQRLWREEGLRVPQRRRRKRV
GSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMADWAGTRTGLF
YIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE
RAAVDHLKRKLRVSERMACRLAGLSRSAYRRPLQGETTADPDLALRDWLRAYAKKHPRWGYRRAYHDARGEGWVVNHKKIQRLWREEGLRVPQRRRRKRV
GSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMADWAGTRTGLF
YIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE
Blast result :
Comments
ISAar43 is 61% aa similar to ISSpwi1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).