ISAar43

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1247 bp

Ends


IR Length : 34/44

IRL : TGGGCTGACCCCGTTTGGTAGGTCCGGTGGTTGTGTGAGTCGTCCTGTTG
IRR : TGGGCCGCCCCCTTCATTCGGTCCGGTCGCTGTGCGAGTCGTCGGTTTCC

Insertion site


Left flankDirect repeatRight flankDR Length
GCGAGCATAAGCCTCCCCCG0

DNA sequence

TGGGCTGACCCCGTTTGGTAGGTCCGGTGGTTGTGTGAGTCGTCCTGTTGCCCGGGGGTCCGGGCAGTGAGACTGGTCAGATCATGACGAACAGCAGGAA
GCGTCACACCTCGGAGCAGGTCGTCCGTAAGCTCGGGCAGGCCGACAGGATGCTCGCGGGCGGTAGCGACATCGCCGGGGTGTGTCGAGAGCTCGGAGTG
TCCGAGCAGACGTACTACCGGTGGCGGAACCAGTACGGCGGGCTGAAGGCCGACGACGCGAAGCGGCTGAAGGAGCTCGAGAAGCAGAACGCCACGCTCA
AGCGGCTGCTCGCCGAGGCAGAGCTCGAGAAGGCCGCGCTCAAGGAGCTGGCTGAGGGAAACTTCTAGGCCCGGGCAGGCGCCGCGCCGCCGTCGACCAC
CTCAAGCGCAAGCTGCGGGTGAGCGAACGAATGGCGTGCCGTCTGGCCGGGCTGAGCAGGTCCGCATACCGTCGCCCGCTCCAGGGTGAGACGACAGCCG
ACCCGGACCTGGCGTTGCGGGACTGGCTGCGGGCGTATGCGAAGAAGCACCCGCGGTGGGGATACCGCAGGGCCTACCACGATGCCCGCGGCGAGGGGTG
GGTCGTGAACCACAAGAAGATCCAACGGCTCTGGCGCGAGGAAGGGCTGCGCGTCCCGCAGCGGCGTCGCCGCAAACGCGTTGGGTCTTCGACCGTCGAC
GCCCCGGCAGCGGTCGCACCGAATCTCGTGTGGGCGGTGGACTTCCAGTTCGACGCGGACGAGCAGGGCCGTCCGATCAAGATCTGCTCCATCGTCGACG
AGCACACCCGCGAGTGCATCGGCGGGCTCGTCGAACGGTCGATCACCGCGGACCGGCTCACCGCCCACCTCGAGGACCTCGTCGCCGTCCGCGGCGCCCC
CGCGGTGCTCCGATCCGACAACGGCCCCGAGTTCATCAGCGACGCGATGGCCGACTGGGCCGGCACCCGCACCGGCCTGTTCTACATCCCGCCCGGCTCG
CCCTGGCACAACGGGTACGTCGAGTCGTTCAACAGCAGGCTCCGCGACGAGTGCCTGAACATCAACAGCTTCTACTCGCTGCTCCACGCCCAGGTCGTGA
TCGGCGACTGGAAGACCGAATACAACCACGACCGCCGGCATTCATCGCTCGGCTACCTCGCACCCGTCGACTACGCTCGGCAATGCACCCATCAATCGGA
AACCGACGACTCGCACAGCGACCGGACCGAATGAAGGGGGCGGCCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
285 bp94 aa84368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTNSRKRHTSEQVVRKLGQADRMLAGGSDIAGVCRELGVSEQTYYRWRNQYGGLKADDAKRLKELEKQNATLKRLLAEAELEKAALKELAEGNF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
879 bp292 aa3561234+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GKLLGPGRRRAAVDHLKRKLRVSERMACRLAGLSRSAYRRPLQGETTADPDLALRDWLRAYAKKHPRWGYRRAYHDARGEGWVVNHKKIQRLWREEGLRV
PQRRRRKRVGSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMAD
WAGTRTGLFYIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1151 bp383 aa841234+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTNSRKRHTSEQVVRKLGQADRMLAGGSDIAGVCRELGVSEQTYYRWRNQYGGLKADDAKRLKELEKQNATLKRLLAEAELEKAALKELAEGKLLGPGRR
RAAVDHLKRKLRVSERMACRLAGLSRSAYRRPLQGETTADPDLALRDWLRAYAKKHPRWGYRRAYHDARGEGWVVNHKKIQRLWREEGLRVPQRRRRKRV
GSSTVDAPAAVAPNLVWAVDFQFDADEQGRPIKICSIVDEHTRECIGGLVERSITADRLTAHLEDLVAVRGAPAVLRSDNGPEFISDAMADWAGTRTGLF
YIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHDRRHSSLGYLAPVDYARQCTHQSETDDSHSDRTE

 

Blast result :
Comments
ISAar43 is 61% aa similar to ISSpwi1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).