ISBf11
- Family IS1380
- Group IS942
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Bacteroides fragilis | Bacteroides fragilis H3 |
DNA section
IS Length : 1594 bp
Ends
IR Length : 13/15
IRL : CCTTAATTCCGCAACACTTTGATTTAACTTTATTTATAAGTACCTGAGCA
IRR : CCTCAAATCCGCAACTACGCGCCTAATATGATACAGAAGCGAAAATAATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATTTTATTC | TTTTA | TATGCTGTAT | 5 |
DNA sequence
CCTTAATTCCGCAACACTTTGATTTAACTTTATTTATAAGTACCTGAGCAACAAAAAGTTGCTCAGGATTTTGCCATGTCAGATTTTTCACCTATCTTAG
TGTTGCAAATCAAAATATGTATCAAACCCGACAGACATGGCAAAGGTAGCAATAAAATCTGAGAAACTCTCTCCTTTTGGAGGTATTTTTCCAATAATGG
AGCAATTTGATTCCAAGCTCTCATCTGTAATCGACTCAACCCTCGGTATGAGATGCAGGCTGTATGGTTATCAATACAGCGAAATCATCCGTTCGCTTAT
GAGCGTTTATTTCTGTGGCGGCTCATGCATCGAGGATGTCACCACTCATCTGATGTATCACCTCTCGCTTCATCCGACACTTCGCACATGCAGTGCCGAC
ACGATTCTCAGAGCCATAAAGGAACTGACCCAGGATAACATTTCCTACACATCCGACACTGGCAAGACCTACGACTTCAACACGGCAGACAAGCTGAATA
CACTGCTCCTCAATTGCCTATTGTCCACAGGGCAGCTGAAAGAGGGCGAGGGGTATGACGTTGACTTCGACCACCAGTTCATAGAGGCTGAGAAGTATGA
TGCGAAGCCTACATACAAGAAGTTCCTTGGTTATCGCCCTGGCGTGGCAGTTATTGGCGACTTGATTGTCGGCATTGAGAACAGCGACGGCAACACAAAT
GTCCGTTTCCACCAGAAGGACACGCTGAAGAGGTTCTTTGAAAGGTTTGAACAGAACAAGCTTATTATCAACCGTTTCAGAGCTGATTGCGGCTCGTGTT
CTGAAGAGATAGTGGAAGAGATTGCTAAACACTGCAAGACCTTCTATATCCGTGCCAACCGATGCAGTTCGCTCTACAATGACATCTTTGCCCTCAGAGG
TTGGAAGAAAGTGGAATTGGGCGGCATTGAGTTTGAGCTGGCCTCCATTCTCATCGAGAAATGGAAAGGGAAGGCATACCGTCTTGTGATTCAAAGACAG
AAGCGGATAGACGGCGAACTCGACCTGTGGGAAGGAGAATACACCTACCGCTGCATCCTTACAAATGACTACGAGTCTTCCGAGAAAGAGATTGTCGAAT
TCTATAACCTCCGTGGAGGGAAGGAACGCATCTTCGATGAAATGAATAACGGATTCGGCTGGAACAGGCTTCCAAAATCCTTCATGGGAGAAAACACGGT
GTTTCTTCTGCTTACGGCACTCATACGCAACTTCTATAAATTCATCATGGCGAGGCTTGACGTGAAGAGGTTCGGACTCAAAGCAACAAGCCGCATCAAG
GCGTTTGTCTTCAAGTTCATCTCTGTGCCAGCCAAATGGGGCAGGACATCAAGGCAGTATGTGCTGAATATCTATTCATGCAACAACGCTTATGCTGATG
TGTTCCAGAATGACTTTGGATAACACCGGCAACTTATGGTCGTGATTTTGCGTATTGCCTCAAGTCGCATGGTGGGGTAAGGGGCATTTATATGTCTTTA
TGGTGACATTCGATGCACTGTGCCCCCTTTTTACTAACGATGCAGATTATTTTCGCTTCTGTATCATATTAGGCGCGTAGTTGCGGATTTGAGG
TGTTGCAAATCAAAATATGTATCAAACCCGACAGACATGGCAAAGGTAGCAATAAAATCTGAGAAACTCTCTCCTTTTGGAGGTATTTTTCCAATAATGG
AGCAATTTGATTCCAAGCTCTCATCTGTAATCGACTCAACCCTCGGTATGAGATGCAGGCTGTATGGTTATCAATACAGCGAAATCATCCGTTCGCTTAT
GAGCGTTTATTTCTGTGGCGGCTCATGCATCGAGGATGTCACCACTCATCTGATGTATCACCTCTCGCTTCATCCGACACTTCGCACATGCAGTGCCGAC
ACGATTCTCAGAGCCATAAAGGAACTGACCCAGGATAACATTTCCTACACATCCGACACTGGCAAGACCTACGACTTCAACACGGCAGACAAGCTGAATA
CACTGCTCCTCAATTGCCTATTGTCCACAGGGCAGCTGAAAGAGGGCGAGGGGTATGACGTTGACTTCGACCACCAGTTCATAGAGGCTGAGAAGTATGA
TGCGAAGCCTACATACAAGAAGTTCCTTGGTTATCGCCCTGGCGTGGCAGTTATTGGCGACTTGATTGTCGGCATTGAGAACAGCGACGGCAACACAAAT
GTCCGTTTCCACCAGAAGGACACGCTGAAGAGGTTCTTTGAAAGGTTTGAACAGAACAAGCTTATTATCAACCGTTTCAGAGCTGATTGCGGCTCGTGTT
CTGAAGAGATAGTGGAAGAGATTGCTAAACACTGCAAGACCTTCTATATCCGTGCCAACCGATGCAGTTCGCTCTACAATGACATCTTTGCCCTCAGAGG
TTGGAAGAAAGTGGAATTGGGCGGCATTGAGTTTGAGCTGGCCTCCATTCTCATCGAGAAATGGAAAGGGAAGGCATACCGTCTTGTGATTCAAAGACAG
AAGCGGATAGACGGCGAACTCGACCTGTGGGAAGGAGAATACACCTACCGCTGCATCCTTACAAATGACTACGAGTCTTCCGAGAAAGAGATTGTCGAAT
TCTATAACCTCCGTGGAGGGAAGGAACGCATCTTCGATGAAATGAATAACGGATTCGGCTGGAACAGGCTTCCAAAATCCTTCATGGGAGAAAACACGGT
GTTTCTTCTGCTTACGGCACTCATACGCAACTTCTATAAATTCATCATGGCGAGGCTTGACGTGAAGAGGTTCGGACTCAAAGCAACAAGCCGCATCAAG
GCGTTTGTCTTCAAGTTCATCTCTGTGCCAGCCAAATGGGGCAGGACATCAAGGCAGTATGTGCTGAATATCTATTCATGCAACAACGCTTATGCTGATG
TGTTCCAGAATGACTTTGGATAACACCGGCAACTTATGGTCGTGATTTTGCGTATTGCCTCAAGTCGCATGGTGGGGTAAGGGGCATTTATATGTCTTTA
TGGTGACATTCGATGCACTGTGCCCCCTTTTTACTAACGATGCAGATTATTTTCGCTTCTGTATCATATTAGGCGCGTAGTTGCGGATTTGAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1287 bp | 428 aa | 137 | 1423 | + | No |
Chemistry : DDE
ORF sequence :
MAKVAIKSEKLSPFGGIFPIMEQFDSKLSSVIDSTLGMRCRLYGYQYSEIIRSLMSVYFCGGSCIEDVTTHLMYHLSLHPTLRTCSADTILRAIKELTQD
NISYTSDTGKTYDFNTADKLNTLLLNCLLSTGQLKEGEGYDVDFDHQFIEAEKYDAKPTYKKFLGYRPGVAVIGDLIVGIENSDGNTNVRFHQKDTLKRF
FERFEQNKLIINRFRADCGSCSEEIVEEIAKHCKTFYIRANRCSSLYNDIFALRGWKKVELGGIEFELASILIEKWKGKAYRLVIQRQKRIDGELDLWEG
EYTYRCILTNDYESSEKEIVEFYNLRGGKERIFDEMNNGFGWNRLPKSFMGENTVFLLLTALIRNFYKFIMARLDVKRFGLKATSRIKAFVFKFISVPAK
WGRTSRQYVLNIYSCNNAYADVFQNDFG
NISYTSDTGKTYDFNTADKLNTLLLNCLLSTGQLKEGEGYDVDFDHQFIEAEKYDAKPTYKKFLGYRPGVAVIGDLIVGIENSDGNTNVRFHQKDTLKRF
FERFEQNKLIINRFRADCGSCSEEIVEEIAKHCKTFYIRANRCSSLYNDIFALRGWKKVELGGIEFELASILIEKWKGKAYRLVIQRQKRIDGELDLWEG
EYTYRCILTNDYESSEKEIVEFYNLRGGKERIFDEMNNGFGWNRLPKSFMGENTVFLLLTALIRNFYKFIMARLDVKRFGLKATSRIKAFVFKFISVPAK
WGRTSRQYVLNIYSCNNAYADVFQNDFG
Blast result :
Comments
ISBf11 activates a metallo-beta-lactamase gene (cfiA) in Bacteroides fragilis strain H3. Together with IS612, IS614, IS942, IS612B, IS614B and an IS element from B. fraglis 417 from Italy they display segmented homology to each other, thus froming a 'cluster' of mosaic IS elements.
ISBf11 is 93% aa similar to IS613.
ISBf11 is 93% aa similar to IS613.
References
1] Jozsef Soki (2009) Direct submission.