ISfinder Mirror Site

ISAva8

Family IS5
Group IS1031

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007413	ND	Anabaena variabilis	Anabaena variabilis ATCC 29413

DNA section

IS Length : 909 bp

Ends

IR Length : 21/23

IRL : GAGGCTATTTATAAAGTAAATCTAAAAGCGAGAGAAAAGGAGTTACGGTA
IRR : GAGATTATTTATAAAGTAAATCTTTAGGAGACAAGACGACGTAGCATAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTATCACGTT	TTA	AATATTGATA	3
AGTTTATGGT	TTA	GCAGCAAAAC	3

DNA sequence

GAGGCTATTTATAAAGTAAATCTAAAAGCGAGAGAAAAGGAGTTACGGTAACGATAAAATACTTCATAAACAGTGTATTTACATAGTTGCTCATGGCTCG
AAAGTCTTACCCCACAGACTTAACTGATATAGAGTGGGAAATTCTGTCTGAGTTGATTCCACCAGCAAAAACTGGAGGACATCCACGCACAACAGATATG
CGTGAAATATGCAATGCCATCTATTATCATTTGAAAACAGGATGTCAATGGAATATGCTTCCAGGGGACTTCCCACCAAGCTCAACGGTATACAGCTACT
ACAGGAAATGGCAGCGCAAGGGAGTTTGGGAAAAATTAAATCATACATTGCGTGGTCAAGTTCGCTCAAAGTTAGGCAAATCAACACAACCAACTGCGAT
CGCCGCAGACAGTCAGTCGGTCAAGACCGACCAAAAAAAGGGGATGTGTACGGTTTTGACGGATGTAAAAAGGTAAAGGGCAGAAAGCGGCAGACTTTGG
TTGATAGTCTAGGACTTTTGTTAAAAGTCGTTGTCAGTGAAGCAAACGCCCCAGAACGAGTACTTGCTGCTTACGCACTCATGGAATTGCTAGAAGAACG
CCCGGAATTACTGGAAAAAGTTAAAGTTATGTGGGTTGATTCTGGTTACGACGGTGATAAATTTGCACTCTGTGTTTGGTTGATGATCCAAGCTCATGTT
GAAGTCATACGGCGTACTGAACAAGAATTTCAAGTTTTGCCTAAACGTTGGGTAGTAGAAAGAACATTTGGGTGGTTTAACCAATACCATCGTCTTAGTA
AAGATTATGAGCGTCTTACCGAAATGAGCGAAGCAGCCATATATGCTGTTATGACTAGAATTATGCTACGTCGTCTTGTCTCCTAAAGATTTACTTTATA
AATAATCTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	87	476	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

LLMARKSYPTDLTDIEWEILSELIPPAKTGGHPRTTDMREICNAIYYHLKTGCQWNMLPGDFPPSSTVYSYYRKWQRKGVWEKLNHTLRGQVRSKLGKST
QPTAIAADSQSVKTDQKKGMCTVLTDVKR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
681 bp	226 aa	206	886	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NMQCHLLSFENRMSMEYASRGLPTKLNGIQLLQEMAAQGSLGKIKSYIAWSSSLKVRQINTTNCDRRRQSVGQDRPKKGDVYGFDGCKKVKGRKRQTLVD
SLGLLLKVVVSEANAPERVLAAYALMELLEERPELLEKVKVMWVDSGYDGDKFALCVWLMIQAHVEVIRRTEQEFQVLPKRWVVERTFGWFNQYHRLSKD
YERLTEMSEAAIYAVMTRIMLRRLVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
800 bp	266 aa	87	886	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

LLMARKSYPTDLTDIEWEILSELIPPAKTGGHPRTTDMREICNAIYYHLKTGCQWNMLPGDFPPSSTVYSYYRKWQRKGVWEKLNHTLRGQVRSKLGKST
QPTAIAADSQSVKTDQKKGDVYGFDGCKKVKGRKRQTLVDSLGLLLKVVVSEANAPERVLAAYALMELLEERPELLEKVKVMWVDSGYDGDKFALCVWLM
IQAHVEVIRRTEQEFQVLPKRWVVERTFGWFNQYHRLSKDYERLTEMSEAAIYAVMTRIMLRRLVS

Blast result :

Comments

ISAva8 is 63% aa similar to ISAcma9.

References

1] ISfinder annotation (2009).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H.,Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P.(2005) Direct Submission.