ISAva7
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007413 | ND | Anabaena variabilis | Anabaena variabilis ATCC 29413 Anabaena variabilis ATCC 29413 plasmid C |
DNA section
IS Length : 909 bp
Ends
IR Length : 21/23
IRL : GAGGCTATTTATAAAGTAAATCTAAAAGTGAGTATAAAAGTAAGCTTGGA
IRR : GAGATTATTTATAAAGTAAATCTTTAGGATACAAGACGACGTAGCATAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAATGATATT | TAA | GGGGTTGCGT | 3 |
| AGGATATCTA | TCA | GGGTGAGGCG | 3 |
| AAATAAAGCT | TTA | GCAAAAGAGC | 3 |
| CTTTTCATCC | TCA | AGCTTTAGCC | 3 |
| AAATAGTTGT | TGA | AATCAAGAGC | 3 |
| TCGCTCTATG | TCA | GCAAAGGTTA | 3 |
| CCAGCAAGTG | TTA | GCTGCTGGAG | 3 |
DNA sequence
GAGGCTATTTATAAAGTAAATCTAAAAGTGAGTATAAAAGTAAGCTTGGAGGTATAGGATACATGAAATATAGTGTATTTACATAGCTACTCATGGGACG
AAAGTCTTACCCCACAGACTTAACTGATATGGAGTGGGAAATTCTGGCTCCATTGATTCCACCAGCCAAAGAAGGAGGGCATCCACGCACAACGGATATG
CGTGAGATATGCAATGCTATCTACTATCACTTGAAAACGGGATGCCAATGGAATATGCTTCCGGGGGACTTTCCACCCAGTTCAACTGTTTACAGTTACT
ACCGCAAATGGCAGCGCAAAGGAGTCTGGGAAAAATTAAACCATACACTGCGCGGACAAGTTCGCACAAAACTAGGCAAATCAACGCAACCCACAGCGAT
CGCCGCAGACAGTCAGTCGGTCAAAACTGACCAAAAAAAGGGGAAGTGTACGGTTTTGACGGGTGTAAAAAGGTAAAAGGAAGAAAGCGGCAGACTCTAG
TTGATAGCCTGGGACTGTTGTTGAAAGTTGTTGTCAGTGAAGCCAATGCGCCGGAACGGGTACTTGCTGCCTACGCATTAATGGAACTTTTAGAGGAACG
TCCGGAATTACTGGAAAAAGTTCAAGTCATGTGGGTTGATTCCGGTTACGACGGCGATAAATTTGCTCTTTCAGTTTGGTTGATGATCCAAGCACATGTT
GAAGTCATACGGCGTACTGAGCAAGAATTCCGGATTTTGCCCAAACGTTGGGTAGTCGAAAGAACATTTGGCTGGTTGAACCAATATCATCGTCTCAGTA
AGGATTATGAGCATCTTCCAGAGATGAGCGAAGCAGCTATATATGCTGTAATGACTAGGATTATGCTACGTCGTCTTGTATCCTAAAGATTTACTTTATA
AATAATCTC
AAAGTCTTACCCCACAGACTTAACTGATATGGAGTGGGAAATTCTGGCTCCATTGATTCCACCAGCCAAAGAAGGAGGGCATCCACGCACAACGGATATG
CGTGAGATATGCAATGCTATCTACTATCACTTGAAAACGGGATGCCAATGGAATATGCTTCCGGGGGACTTTCCACCCAGTTCAACTGTTTACAGTTACT
ACCGCAAATGGCAGCGCAAAGGAGTCTGGGAAAAATTAAACCATACACTGCGCGGACAAGTTCGCACAAAACTAGGCAAATCAACGCAACCCACAGCGAT
CGCCGCAGACAGTCAGTCGGTCAAAACTGACCAAAAAAAGGGGAAGTGTACGGTTTTGACGGGTGTAAAAAGGTAAAAGGAAGAAAGCGGCAGACTCTAG
TTGATAGCCTGGGACTGTTGTTGAAAGTTGTTGTCAGTGAAGCCAATGCGCCGGAACGGGTACTTGCTGCCTACGCATTAATGGAACTTTTAGAGGAACG
TCCGGAATTACTGGAAAAAGTTCAAGTCATGTGGGTTGATTCCGGTTACGACGGCGATAAATTTGCTCTTTCAGTTTGGTTGATGATCCAAGCACATGTT
GAAGTCATACGGCGTACTGAGCAAGAATTCCGGATTTTGCCCAAACGTTGGGTAGTCGAAAGAACATTTGGCTGGTTGAACCAATATCATCGTCTCAGTA
AGGATTATGAGCATCTTCCAGAGATGAGCGAAGCAGCTATATATGCTGTAATGACTAGGATTATGCTACGTCGTCTTGTATCCTAAAGATTTACTTTATA
AATAATCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 93 | 476 | + | No |
Description : First part of the transposase
ORF sequence :
MGRKSYPTDLTDMEWEILAPLIPPAKEGGHPRTTDMREICNAIYYHLKTGCQWNMLPGDFPPSSTVYSYYRKWQRKGVWEKLNHTLRGQVRTKLGKSTQP
TAIAADSQSVKTDQKKGKCTVLTGVKR
TAIAADSQSVKTDQKKGKCTVLTGVKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 456 bp | 151 aa | 431 | 886 | + | No |
Description : Second part of the transposase
ORF sequence :
PKKGEVYGFDGCKKVKGRKRQTLVDSLGLLLKVVVSEANAPERVLAAYALMELLEERPELLEKVQVMWVDSGYDGDKFALSVWLMIQAHVEVIRRTEQEF
RILPKRWVVERTFGWLNQYHRLSKDYEHLPEMSEAAIYAVMTRIMLRRLVS
RILPKRWVVERTFGWLNQYHRLSKDYEHLPEMSEAAIYAVMTRIMLRRLVS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 794 bp | 264 aa | 93 | 886 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRKSYPTDLTDMEWEILAPLIPPAKEGGHPRTTDMREICNAIYYHLKTGCQWNMLPGDFPPSSTVYSYYRKWQRKGVWEKLNHTLRGQVRTKLGKSTQP
TAIAADSQSVKTDQKKGEVYGFDGCKKVKGRKRQTLVDSLGLLLKVVVSEANAPERVLAAYALMELLEERPELLEKVQVMWVDSGYDGDKFALSVWLMIQ
AHVEVIRRTEQEFRILPKRWVVERTFGWLNQYHRLSKDYEHLPEMSEAAIYAVMTRIMLRRLVS
TAIAADSQSVKTDQKKGEVYGFDGCKKVKGRKRQTLVDSLGLLLKVVVSEANAPERVLAAYALMELLEERPELLEKVQVMWVDSGYDGDKFALSVWLMIQ
AHVEVIRRTEQEFRILPKRWVVERTFGWLNQYHRLSKDYEHLPEMSEAAIYAVMTRIMLRRLVS
Blast result :
Comments
ISAva7 is 64% aa similar to ISAcma9.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H.,Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P.(2005) Direct Submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H.,Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P.(2005) Direct Submission.