ISAar40
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1347 bp
Ends
IR Length : 20/25
IRL : TGGGGTTAGCCTGAATTAGTGGACAGCCGAACAAGCGGGATCAGCCGATC
IRR : TGGGGTTCCCCACAACTAGTGGACACGTGATCTAGCTCATGCTGCTAGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTCCTGGC | AGTGCGCCAG | 0 |
DNA sequence
TGGGGTTAGCCTGAATTAGTGGACAGCCGAACAAGCGGGATCAGCCGATCCCGCATTTGAGAGGATGTCATTAATGAGCAATATGAAAAAGCAACGCAGG
TCCTTCACCGAGGAGTACCGCAGGGAAGCTGCCGGCCTGGTCATCGATACCGGACGCACCATCAGCGCGGTCGCCAAGGAACTGAATCTCGGCGAGCAAA
CACTGGGCACCTGGGTGAAGAAAGAACGATCCCGCCGAGGCCTGGACGGTGAGCCGACCGGAGCGTTGGACGAGTCCGAACGCGAAGAACTCAAACGCTT
GCGCAAGGAAGTCTTCGAACTGCGGGAGGACAACCGTTTCCTGGGAAAAGCAGCGTCCTTCTTCGCGTCGAAGCAACATCGGTCGAACGGTTCGAACTGA
TGCACGCGTTGAAGGATGAGTTTGCCTTGACCCGCATGGCGTCATTGCTTCACGTTTCGAAATCAGGCTATTACGCCTGGAGGCAACGCCAATCCGCTGG
CCCGTCCCCGAGGGGTGCCGCGCAGCGGGCACGGGATGTGAAGGTCGCCAAGGTCTTCAACGATTCCCATCAGACCTACGGGGCTCCGCGGGTGGCCGCC
CAGCTGGCCCGCGAGGGATCCCCGGCCGATCCCAAGACCGTCGCTGCTTCAATGCTGCGCCAGGGCATCGAAGGGATCAGCCCACGGAAGTTCACTCCGG
TGACCATGATCCAAGGCGTTGACACTTACCACCTGCCGGATCGGGTGCGCCGGCAATGGGACCAGGGCACCTTGGACAAGGTCTGGATCAGCGACATTAC
GTATCTGCGCATCAACGAGGGCTGGCTGTACTTGTGCGCAGTGCGTGATGGCTGCTCCCGCAGGGTGCTGGGATGGGCGCTGGATAGCGTGCAGAACACC
GATCTGGTGGAAAGGGCCCTGCGCATGGCCAGGACCCTGCGCGGCCAGATTCCAGGTCAGGTGGTGTTCCACGCGGACCGCGGCTCGCAATTCACCTCAG
CTCAGTTGCATGAGGTGGCCGTTGAGCTGGATTTGCTGCAGTCCGTGGGCCGCACGGGCGTGTGCTGGGACAACGCCATGAGCGAGTCATTCTGGTCGAC
GCTGAAGACCGAATTCTATGACCGCTATCGGTGGGCAACCAGGGCGGACGCCAAGCAGAAAGTGGCTTGGTGGATTGAGGATTTCTATAACCGCCGCCGC
CGGCTGCATTCAAGCCTGGGGATGGTTCCTCCGGTGGAGTTTGAGCAGAAGCTTCGCGGTCAGCAGGAACGCGTTGAACAGCATAGGACCGTGCTAACGC
TAGCAGCATGAGCTAGATCACGTGTCCACTAGTTGTGGGGAACCCCA
TCCTTCACCGAGGAGTACCGCAGGGAAGCTGCCGGCCTGGTCATCGATACCGGACGCACCATCAGCGCGGTCGCCAAGGAACTGAATCTCGGCGAGCAAA
CACTGGGCACCTGGGTGAAGAAAGAACGATCCCGCCGAGGCCTGGACGGTGAGCCGACCGGAGCGTTGGACGAGTCCGAACGCGAAGAACTCAAACGCTT
GCGCAAGGAAGTCTTCGAACTGCGGGAGGACAACCGTTTCCTGGGAAAAGCAGCGTCCTTCTTCGCGTCGAAGCAACATCGGTCGAACGGTTCGAACTGA
TGCACGCGTTGAAGGATGAGTTTGCCTTGACCCGCATGGCGTCATTGCTTCACGTTTCGAAATCAGGCTATTACGCCTGGAGGCAACGCCAATCCGCTGG
CCCGTCCCCGAGGGGTGCCGCGCAGCGGGCACGGGATGTGAAGGTCGCCAAGGTCTTCAACGATTCCCATCAGACCTACGGGGCTCCGCGGGTGGCCGCC
CAGCTGGCCCGCGAGGGATCCCCGGCCGATCCCAAGACCGTCGCTGCTTCAATGCTGCGCCAGGGCATCGAAGGGATCAGCCCACGGAAGTTCACTCCGG
TGACCATGATCCAAGGCGTTGACACTTACCACCTGCCGGATCGGGTGCGCCGGCAATGGGACCAGGGCACCTTGGACAAGGTCTGGATCAGCGACATTAC
GTATCTGCGCATCAACGAGGGCTGGCTGTACTTGTGCGCAGTGCGTGATGGCTGCTCCCGCAGGGTGCTGGGATGGGCGCTGGATAGCGTGCAGAACACC
GATCTGGTGGAAAGGGCCCTGCGCATGGCCAGGACCCTGCGCGGCCAGATTCCAGGTCAGGTGGTGTTCCACGCGGACCGCGGCTCGCAATTCACCTCAG
CTCAGTTGCATGAGGTGGCCGTTGAGCTGGATTTGCTGCAGTCCGTGGGCCGCACGGGCGTGTGCTGGGACAACGCCATGAGCGAGTCATTCTGGTCGAC
GCTGAAGACCGAATTCTATGACCGCTATCGGTGGGCAACCAGGGCGGACGCCAAGCAGAAAGTGGCTTGGTGGATTGAGGATTTCTATAACCGCCGCCGC
CGGCTGCATTCAAGCCTGGGGATGGTTCCTCCGGTGGAGTTTGAGCAGAAGCTTCGCGGTCAGCAGGAACGCGTTGAACAGCATAGGACCGTGCTAACGC
TAGCAGCATGAGCTAGATCACGTGTCCACTAGTTGTGGGGAACCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 74 | 400 | + | No |
Description : First part of the transposase
ORF sequence :
MSNMKKQRRSFTEEYRREAAGLVIDTGRTISAVAKELNLGEQTLGTWVKKERSRRGLDGEPTGALDESEREELKRLRKEVFELREDNRFLGKAASFFASK
QHRSNGSN
QHRSNGSN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1011 bp | 336 aa | 301 | 1311 | + | No |
Description : Second part of the transposase
ORF sequence :
ADRSVGRVRTRRTQTLAQGSLRTAGGQPFPGKSSVLLRVEATSVERFELMHALKDEFALTRMASLLHVSKSGYYAWRQRQSAGPSPRGAAQRARDVKVAK
VFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIEGISPRKFTPVTMIQGVDTYHLPDRVRRQWDQGTLDKVWISDITYLRINEGWLYLCAVRDG
CSRRVLGWALDSVQNTDLVERALRMARTLRGQIPGQVVFHADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADA
KQKVAWWIEDFYNRRRRLHSSLGMVPPVEFEQKLRGQQERVEQHRTVLTLAA
VFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIEGISPRKFTPVTMIQGVDTYHLPDRVRRQWDQGTLDKVWISDITYLRINEGWLYLCAVRDG
CSRRVLGWALDSVQNTDLVERALRMARTLRGQIPGQVVFHADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADA
KQKVAWWIEDFYNRRRRLHSSLGMVPPVEFEQKLRGQQERVEQHRTVLTLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1238 bp | 412 aa | 74 | 1311 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNMKKQRRSFTEEYRREAAGLVIDTGRTISAVAKELNLGEQTLGTWVKKERSRRGLDGEPTGALDESEREELKRLRKEVFELREDNRFLGKSSVLLRVE
ATSVERFELMHALKDEFALTRMASLLHVSKSGYYAWRQRQSAGPSPRGAAQRARDVKVAKVFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIE
GISPRKFTPVTMIQGVDTYHLPDRVRRQWDQGTLDKVWISDITYLRINEGWLYLCAVRDGCSRRVLGWALDSVQNTDLVERALRMARTLRGQIPGQVVFH
ADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRRRLHSSLGMVPPVEFEQKLRGQQER
VEQHRTVLTLAA
ATSVERFELMHALKDEFALTRMASLLHVSKSGYYAWRQRQSAGPSPRGAAQRARDVKVAKVFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIE
GISPRKFTPVTMIQGVDTYHLPDRVRRQWDQGTLDKVWISDITYLRINEGWLYLCAVRDGCSRRVLGWALDSVQNTDLVERALRMARTLRGQIPGQVVFH
ADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRRRLHSSLGMVPPVEFEQKLRGQQER
VEQHRTVLTLAA
Blast result :
Comments
ISAar40 is 97% aa similar to ISAar26.
ISAar40 was reconstructed in silico by deletion of ISAar41. The transposase is not affected by insertion of ISAar41.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAar40 was reconstructed in silico by deletion of ISAar41. The transposase is not affected by insertion of ISAar41.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).