ISfinder Mirror Site

ISAva6

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007413	ND	Anabaena variabilis	Anabaena variabilis ATCC 29413 Anabaena variabilis ATCC 29413 plasmid A

DNA section

IS Length : 1161 bp

Ends

IR Length : 26/29

IRL : CTGAATAGGGAAAGGTAGTATCATTTGAAGCAGAGGGAAAAAGGCTAGAA
IRR : CTAAATTGGGAAAGGAAGTATTAATTGAATAACTGAGGTCAAATTTGGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCCCTCTGA	TA	TGATTGTGTA	2
CTAAAGATTA	TA	TTAGATCCGG	2
ACACAGATAA	TA	TAAGACATAG	2
GCATAACTAA	TA	TAAGAATGAA	2
GAATGAATAA	TA	TAATTTCTTG	2
CTTTGTGTTA	TA	TTAGAGACTC	2
TCACCTGTTA	TA	TTATGCCTAC	2
ATTGGTATTA	TA	TTACTCCCTA	2
ATTGGTATTA	TA	TTATGTCCCC	2
TGACTATTGA	TA	TGACTATTGA	2
ATACTTATGA	TA	TGAAATATGA	2
CTGCTGCTTA	TA	TTACTAAGAA	2
GGGTGTCTTA	TA	TTATGCCGCA	2

DNA sequence

CTGAATAGGGAAAGGTAGTATCATTTGAAGCAGAGGGAAAAAGGCTAGAAAAATGGTACGTCCCAGGATAAAACTAACAGAGCATCTATCAACAGAAGAA
ATAGAACAAAGTTATCGCCGATGTGAAGATGCACAAGAGAAAACCCGATGGTTAGTGATTAAATTGCTCAATCAACAACCACAGTTGTCAGCGCAAAAGG
TAGCAGAGATTGTGGGATTCTCAGGGGACTGGGTGAGAAAAATCGTGCGGCGATACAACAAGCTAGGAGCAAACGGAATCATCAATCAACAGAAACTGAA
ACCAGGAGGAAAAAAACTTGCACTCACAAACGAGCAACAACAGTGGTTGCGCCAAAGGTTAGCTTCACCACCAGAAGATGGGGGGTTATGGAGTGCGCCA
AAAGTAGGGGAATTGATCCGAGTACAGTTTGGAATTACACTCCATGTCACTACAGCTTGGGATTACCTCAAAAGGCTAGGATTTAGTCTGCAACAACCAC
GACCTCTGCATACTGAGGCGGCAACTTTTGTTCAAAGACAGATGTTTAAAACTGAGTTAACGGAGTTTGTGCGATTGTTACGTTTCCTCCATCCGCACAA
ATCAGTTGAAGTTTGGGCAGAAGATGAAGCTCGATTAGGCCTCAAACCCATCGTCCGTCGAGTTTGGACACCAGTAGGTCATCGTCCCAATGCTGTGCAT
CGCACTCGTTACCAATGGCTTTATACTTATGGATTTGTCCATCCAGCTACTGGCGAGAGTTTTTTCTTGATTTTACCCAGAGTCAACATTGCTGTCATGC
AAATGGCTTTAGATGCTTTTGCCGCTGAAGTCAATCCTCATCATCACAAAATCATTGTTTTGCTTGTTGATCAAGCTGGTTGGCATACCAGTAAACAATT
AATGTTGCCAGCTGGTATCATTCTGTTTCCTCTGCCTGCTTATACACCTCAACTCCAACCGACTGAGTGTGTTTGGTCGCTTCTACGTGAAGCTGTTGCT
AATCAGATGTTTTCTACTCTCGATGAACTAGAAACTGTGTTAATTTCTCGTTGTCAATGGTTAATGTCTCACCCTCAAATTGTTCATGGCAAGGTTGGGT
TTGATTGGATTTGCCAAATTTGACCTCAGTTATTCAATTAATACTTCCTTTCCCAATTTAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1071 bp	356 aa	53	1123	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVRPRIKLTEHLSTEEIEQSYRRCEDAQEKTRWLVIKLLNQQPQLSAQKVAEIVGFSGDWVRKIVRRYNKLGANGIINQQKLKPGGKKLALTNEQQQWLR
QRLASPPEDGGLWSAPKVGELIRVQFGITLHVTTAWDYLKRLGFSLQQPRPLHTEAATFVQRQMFKTELTEFVRLLRFLHPHKSVEVWAEDEARLGLKPI
VRRVWTPVGHRPNAVHRTRYQWLYTYGFVHPATGESFFLILPRVNIAVMQMALDAFAAEVNPHHHKIIVLLVDQAGWHTSKQLMLPAGIILFPLPAYTPQ
LQPTECVWSLLREAVANQMFSTLDELETVLISRCQWLMSHPQIVHGKVGFDWICQI

Blast result :

Comments

ISAva6 is 48% aa similar to ISAli2.

References

1] ISfinder annotation (2009).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H.,Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P.(2005) Direct Submission.