ISAar33

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 2628 bp

Ends


IR Length : 45/50

IRL : TGAATATTTGACTCTTTGAGAGCCACCAATATTGCGATTCAGAGCCGCCC
IRR : TGAATATTTGACTCTTTGAGAGCCACCAATATTCCCACGCAGAGCCACCC

Insertion site


Left flankDirect repeatRight flankDR Length
ATGGTGCGCATGAAGAACCAAAAT4

DNA sequence

TGAATATTTGACTCTTTGAGAGCCACCAATATTGCGATTCAGAGCCGCCCCGTCGAAATGACGTTATTGTCCGTCAGAGCCACCCAATATTCTCCTTCAG
AGCCACCCTGTATAAACTCCTCCCACTACGCAACAGCCTGCGTGGTGGAAGGAGCCATTTTCAATGGTACGAAAGATCAAAGCGAAGCGAATACTGCAGC
TTCGCGCCGAGGGCTTGTCAGGACGCACCATCGCGTCCACCCAAGGCGTTTCCCGCAATAGCGTCGCAGAAGTCCTCAACGCTGCCGCCGCCAACTCCAG
CAGCTGGGACGAACTCAAGGACCTCACCGAAGACAAAGTCTACGAACTCCTGTTCCCTGGCCGCAGCGAGCACGAAAGCGTGTTCGCCCAACCGGACTGG
CCCACCGTGCATAAGGAACTGGCCAAAGTCGGCACCAACCTCAAGCTCCTGCACGGCGAATACTCCGATCAGGCCAAAGCCACCGGCGCAGCATTCATGG
GTTACGACCGGTTCTGCAAGTCCTACCAACGCTACGTCCTGGAACACGGGGCCACCTCACGAGTGGAGCATAAATCCGGTGTCAGCGTCGAAGTGGACTG
GTCAGGTCCAACCATGGCCCTGCATGACCCGGTCACTGGCCGACGATCCACGGTGTATTTGTTCGTCGCCTGCCTGCCGTTCAGCCGGTACGCGTTCGTT
GAGCCCTGCCTCGATATGAAGCAGGAATCCTGGATGCTATGCCACGCAGCGATGTTCAACGCTTTCGGCGGCAGCGTGCCGCGGATCGTGTGCGACAACC
TGAAAACCGGGGTGATCAAGCACCCGGCGGAAGGGGAGATTATCCTCAACGATGCCTACCGTCACCTCGCCGAGCACTATTCTTCCGCGATCCTGCCAGG
CAGAGTCCGCAGGCCAAAAGACAAATCCAGCGTGGAAAATACCGTGGGTCATGTGGCTACGTGGGTGATCGCTTCACTACGCCATGAAAAGTTCACCACG
CTTGATTCCCTGCGTGCGGCCATCTATCGGCAGGCAGAAGCCTATAACGCGCAACCCTTCCAGAAGCGTGCCGGCTCCCGCCAGAGTGTTTTCCGCGAGG
AAGAGCAGCCACTGCTGCGTCCGCTCCCAGTGGTGCCCTACGAGATCAGCACGTGGGTCCACGGACGCAAGGTCGCCAGGAATAGCTACGTGACGTGGAA
GAAGAACTTCTACTCCGTGCCGCTCAAACACGTCGGCGCCACCGTCGACCTGAGGATCACGTCCAAGACCCTGGAAGTCTATCTTCAGTCCCAACGGCTG
AGCAGTCACCTCTTGTGCAATGCGGCCACGGTGAATCAGTACCGGACCAATGATTCCGATATCCCACCAGAACGCAAATACCGCTCGTGGGACCCCAAAC
GGCTCCGCGGATGGGCTCACCGCATCGGCCCGAACACGGTGCAGGTGATCGATAAGATCTTCATTTCCGTGCCGGTTGCCGAGCAAGGAATCAACCCCGC
CCTGGCGGTGCTGCGCTTGTCTTCAAAATACAGTCCACAACGACTGGAGGACGCGTGCTTCGTCGCATTGCAGTCACGGATCCGCTCCCCACGCTACGGG
CATTTGCATCCGATTTTGCATAACAGGCAGGACGAGAACTGGAAAGAAGCGACCCTCCCACCAGCAGCTGAAGACAGCACTGGATACGTACGAGGCAGCG
ACTACTACGCAAGGAAGACCCGATGAACCTGAACGCCGAAACCAAGCGCAAACTCCGTGAGATGGCCGCAGGTGACCTGCTCACGGCATTCGAATCCCAG
GACGATGTGGTGAGCATGTCATTAAGTATCGAAGCACGGATCGAGCTGGCCGTGGACCAAGCCCACGGGCTATTTCTGAATACGAAGTCCCACGGTCTAA
TACGTCGGGCTAAGCTCCGTTATCCGCAGGCTGACTTGCGAAAAGTGGACCGGGTGGAAGAGCGGGGCCTGAACCAGCCGCTGCTGGCCCAGTTGGCGAC
CTGCCATTTCATTGAGCTCAACAGGAATCTGGTTTTCCAGGGTTTTACGGGGTCAGGCAAGTCGTATCTGGCGTGCGCCGTGGCGAAGCAGGCCCGTGTT
CACAGCTACCGTACCGGATACGTGCGGATGCCGGACCTCGAAGAAGAATGGCAGCAGGCCGCCAACAAGCCGTTGGGCGTGCAAAAGCTGTTGCGCAGGT
ATGCCAATTACAGTGCTCTGGTATTGGACGAATGGCTTTTGGAGCCACCGAGTGGAGAGTTCCTGAGTTTCATCTTCGAGCTCATGGAGCGAAGGTACGA
CGCCGCGTCGACTATTTTCTGCACTCAGTACAAGCAGGCCGATTGGCATGCCCGGTTGGGTGCTGGTGCGTTGGCCGACGCAATCATGGACCGCATCGTG
CACAACACGATCTGGATTGAGACCGGTGGGTTCAACATGCGCGAGGCGAACATGAGTGCTGGGCACTAAGCGCATCAAGCGTCCACGGTGAGATTCCTTG
TTCAGAGCCGCCGGTCAACCGTCAAGGCTGGACGGTGGCTCTGAACGAGAATATTGGGTGGCTCCGAACCGCAATAACGGGTGGCTCTGCGTGGGAATAT
TGGTGGCTCTCAAAGAGTCAAATATTCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1563 bp520 aa1641726+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVRKIKAKRILQLRAEGLSGRTIASTQGVSRNSVAEVLNAAAANSSSWDELKDLTEDKVYELLFPGRSEHESVFAQPDWPTVHKELAKVGTNLKLLHGEY
SDQAKATGAAFMGYDRFCKSYQRYVLEHGATSRVEHKSGVSVEVDWSGPTMALHDPVTGRRSTVYLFVACLPFSRYAFVEPCLDMKQESWMLCHAAMFNA
FGGSVPRIVCDNLKTGVIKHPAEGEIILNDAYRHLAEHYSSAILPGRVRRPKDKSSVENTVGHVATWVIASLRHEKFTTLDSLRAAIYRQAEAYNAQPFQ
KRAGSRQSVFREEEQPLLRPLPVVPYEISTWVHGRKVARNSYVTWKKNFYSVPLKHVGATVDLRITSKTLEVYLQSQRLSSHLLCNAATVNQYRTNDSDI
PPERKYRSWDPKRLRGWAHRIGPNTVQVIDKIFISVPVAEQGINPALAVLRLSSKYSPQRLEDACFVALQSRIRSPRYGHLHPILHNRQDENWKEATLPP
AAEDSTGYVRGSDYYARKTR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
747 bp248 aa17232469+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLNAETKRKLREMAAGDLLTAFESQDDVVSMSLSIEARIELAVDQAHGLFLNTKSHGLIRRAKLRYPQADLRKVDRVEERGLNQPLLAQLATCHFIELN
RNLVFQGFTGSGKSYLACAVAKQARVHSYRTGYVRMPDLEEEWQQAANKPLGVQKLLRRYANYSALVLDEWLLEPPSGEFLSFIFELMERRYDAASTIFC
TQYKQADWHARLGAGALADAIMDRIVHNTIWIETGGFNMREANMSAGH

 

Blast result :
Comments
ISAar33 orfA (transposase)is 51% aa similar to ISPre4 and orfB (helper of transposition)is 52% aa similar to ISRme9.
References
1] ISfinder annotation (2009)