ISfinder Mirror Site

ISAcma50

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 plasmid pREB3 Acaryochloris marina MBIC11017 plasmid pREB4 Acaryochloris marina MBIC11017 plasmid pREB5

DNA section

IS Length : 2718 bp

Ends

IR Length : 16/23

IRL : AGTTGAGTGACAATTACGTAGACAGAGGTTGTCAATTGCATAGTCTTCTC
IRR : TGTTTAATGTCAATTAGATAGGCCAAACTTTTTGGTTCTAACTTCATACT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAGATGAGCG	CCTATGAT	GCATTCCCTC	8
CTGAAGTGAA	CCCCTG	AACTTGGACA	6
CAGTGGAGGT	GAATGC	TATCGAATCC	6

DNA sequence

AGTTGAGTGACAATTACGTAGACAGAGGTTGTCAATTGCATAGTCTTCTCTAGGAATCAGGATCTAGTTCAATCCAGAGATCACATCTGCAGAGAGAGCA
TCAGTCACTTTCGATTTTTTATATCCCCATACAGGAAAGACACTAGGGTTTGTACTCCTACGCAATTAGGAACCTCTAGTGCCAGCTCCTATTAAACCTC
AACAGGTTCATCTTTATATGCAGACAAAACAATCCGGCTCTTCTCAAGAAACAGCAGCTGCTAAAGCTGGCATTTCTACCCGCACAGCCCACCGCATTGA
TAGTGGTACTCATCGTCCCCAACGCGGCCGCCCTCATGACTGGAAAACCCGTGAAGATCCGCTGGATGGATTATGGGAATTAGAACTTGAGCCGATGTTA
GAGCGTGAGCCTCGATTAGAAGCGACTACTCTATTTGAGACCTTACAAGAGAACCATCCTGGGCAATATGATGACAAGATCAGAACCGTGCAACGCCGAA
CCGCTAAATGGAAGGCAGCTCATGGCAAGCCCAAAGAAGTGATGTTCAAAATTCAACATCATCCTGGGGAGATGGGACAGTCAGACTTCGCTCAGCTCAA
AGGTTTCAGTGTGACGGTTCAGGGTGAAGCCTTTCACCATATCTTGTATCACTATCGACTGAGCTATAGCGGCTGGCAGTACGTGCAGGTGATTCAAGGC
GGAGAAAGTTTTATCGGCTTATCCCAAGGGCTACAAAATGCTCTAACCGCCTGTGGTGGCGTGCCCAAGATTCATCGTACCGATAGTCTGAGTGCGGCTT
ATCGCAATACTGGCGGCCGCAATCCCCAGCTGACCCAGTTGTATTCGACTCTGTGTGACCATTACCGGATGCAGCCAACACGCAACAATCTGGGTGTCTC
TCATGAGAATGGCGGAATAGAGGGGTCTCATGGCTATTTCAAACGACGCCTGTGCCAAGCCCTGTATCGTCGAGGCAGCTTTGACTTTGACTCGGTAGCT
GAGTATCAGCAGTTCATCGAGCAAGTCATCGCCAAGCTGAATGCCAAGTGCCAAAAAAAGTTTGCACTGGAGCTGCCCACCTTACAGCCCTTGCCCAGAT
ACCGAACTCCTGATTATGAAGTGCGCAGTGCCAAAGTCAGCTGCAATAGCACGATTGCCGTCCGCTGTGTCCTTTACACAGTCCCCTCTCGCTTAATTGG
CCACCGCTTGAAATTACACCTATACCATGACCGCCTTGTCGGTTTCTTAGGCACCACTCCCGTAGTGGAATTGGCGCGGGTCCATGTCCATGGCTCTGAG
AAGAAAAGACGCGCTCGCAGCATCGATTACAAGCATGTGGCAGAAAGTCTCAGGAGAAAGCCAAACGCGTTTCTCTACTGCCAATGGCAATCAGAGCTAC
TGCCCAATCTTCAGTGGCATCAGCTGTGGGAATCACTCAAAGCTAATTTTGAGCAGGACCAGGCCGCACGATTAATCACCGAAGCCCTCTATATCGCTGC
AACTCAAGATCAAGAATCTCAGGTGGCTGACTACCTACAAACTCAACTGGAACAGTCCACCCTGACCTTGAGCAGATTAAAACAAGCCTTTGAATTCAAG
CTCTCGACTGAGCAATATCCTGACGTCACCTCACAACAGCACGATTTATCTGACTATGATCAACTCCTCCACAGCAGACCCGGACAACCCATACCAGTTG
CTGATGACAACCCTAAAACAGTTGCGGTTGAGGCATTTCCTCGACGAGTGGCAGAGCATCGAGCATCAAGCGACTCAGGAGAATTGGTCCTACGCCCAGT
TTCTGTTGGCTTTAGCTCAAGGCGAAGCCAGCCGTCGGGAGCAGAATCGGATTTCACGCGCTCTAACCGAAGCGCAGCTGCCTTACGGAAAGTCCTGGAC
CAATTTTGAGTTTGCCCATGTTCCCACCCTTAATCCAGCTGCTCTCATGGAGTTTGCCCAAACGACTCACTGGCTAGAGTCCGGTAGCAACATTCTAATT
TTTGGGCCGAGCGGAACAGGGAAAACACACGTCAGCTCTGCATTAGGAGCTTCTATTATTGAGTTGGGTAAGCGAGTAAAATTTGTCACAGCCACAACCC
TGGTGCAACAACTACAGCTGGCCAAACTTCAATTGGAATTGCAGAGTCTCTTGTCCAAACTTGACCGCTATGATCTGCTTATCATTGATGATCTCGGTTA
TGTCCAAAAGTCCGATGTTGAAACCTCGGTACTGTTTGAGTTGATTGCTCACCGGTATGAGCGAAAGAGCCTACTCATCACGGCTAATCAACCTTTCAGT
CAATGGGACAGCATCTTTCCTAACTCAATGATGACCGTGGCTGCCATTGATCGCTTAATTCACCACGCCACCATTTTCGAGATGCAAGCAGAAAGCTTCC
GCAAACAAGAAGCGGCCAAACACACAGCCAAGTCTCAAAAGACTGACAGCTGATCTCTAGTCAGAATAAAACCTCTGTACCCCACTATTAATAGAGTTGC
TTACTTATAAGCAACTCTATTAATAGTGGCTTTATTGATAATTGTCATTTTGTCCATGGCGCGATCACCTGAGGTAAAAACCATGATTTTCGATGGTCTC
CGACCGCTCTTCGGGCATCTCAAGATGATATTGCAAAGATCTAAATCGACTCACGAGCCGCTATTAAAAGTATGAAGTTAGAACCAAAAAGTTTGGCCTA
TCTAATTGACATTAAACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1731 bp	576 aa	179	1909	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VPAPIKPQQVHLYMQTKQSGSSQETAAAKAGISTRTAHRIDSGTHRPQRGRPHDWKTREDPLDGLWELELEPMLEREPRLEATTLFETLQENHPGQYDDK
IRTVQRRTAKWKAAHGKPKEVMFKIQHHPGEMGQSDFAQLKGFSVTVQGEAFHHILYHYRLSYSGWQYVQVIQGGESFIGLSQGLQNALTACGGVPKIHR
TDSLSAAYRNTGGRNPQLTQLYSTLCDHYRMQPTRNNLGVSHENGGIEGSHGYFKRRLCQALYRRGSFDFDSVAEYQQFIEQVIAKLNAKCQKKFALELP
TLQPLPRYRTPDYEVRSAKVSCNSTIAVRCVLYTVPSRLIGHRLKLHLYHDRLVGFLGTTPVVELARVHVHGSEKKRRARSIDYKHVAESLRRKPNAFLY
CQWQSELLPNLQWHQLWESLKANFEQDQAARLITEALYIAATQDQESQVADYLQTQLEQSTLTLSRLKQAFEFKLSTEQYPDVTSQQHDLSDYDQLLHSR
PGQPIPVADDNPKTVAVEAFPRRVAEHRASSDSGELVLRPVSVGFSSRRSQPSGAESDFTRSNRSAAALRKVLDQF

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
750 bp	249 aa	1704	2453	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTTLKQLRLRHFLDEWQSIEHQATQENWSYAQFLLALAQGEASRREQNRISRALTEAQLPYGKSWTNFEFAHVPTLNPAALMEFAQTTHWLESGSNILIF
GPSGTGKTHVSSALGASIIELGKRVKFVTATTLVQQLQLAKLQLELQSLLSKLDRYDLLIIDDLGYVQKSDVETSVLFELIAHRYERKSLLITANQPFSQ
WDSIFPNSMMTVAAIDRLIHHATIFEMQAESFRKQEAAKHTAKSQKTDS

Blast result :

Comments

ISAcma50 orfA (transposase) and orfB (helper of transposition) are 67% and 78% aa similar to ISAcma26.
This IS has atypic IRL: it begins with 'AG' instead of 'TG'(ponctual mutation?).

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)