ISAcma38
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009925 | ND | Acaryochloris marina | Acaryochloris marina MBIC11017 plasmid pREB2 |
DNA section
IS Length : 2708 bp
Ends
IR Length : 11/15
IRL : TGGCCAGTTGAGTGACAATTACGTAGACAGAGGTTGTCAATTGCATAGTC
IRR : TGGCCAGATGTCTAATGTCAATTAGATAGGCCAACTTTTTTTGCTCTGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGATCACGTC | TGAAGTCAAT | 0 |
DNA sequence
TGGCCAGTTGAGTGACAATTACGTAGACAGAGGTTGTCAATTGCATAGTCTTCTCTAGGAATCAGAATCTAGTTCAATCCAGAGATCACATCTGCAGAGA
GAGCATCAGTCACTTTCGATTTTTGATAGCCCCATACAGGAAAGACACTAGGGTTTGTACTCCTACGCAATTAGGAACCTCTAGTGCCAGCTCCTATTAA
ACCTCAACAGGTTCATCTTTATATGCAGACAAAGCAATCCGGCTCTTCTCAAGAAACAGCAGCTGCTAAAGCCGGCATTTCTACCCGCACAGCCCACCGC
ATTGATAGTGGTACTCATCGTCCCCAACGAGGCCGCCCTCATGACTGGAAAACCCGTGAAGATCCGCTGGATGGATTATGGGAATTAGAACTTGAGCCGA
TGTTAGAGCGTGAGCCTCGATTAGAAGCGACTACTCTATTTGAGACCTTACAAGAGAACCATCCTGGGCAATATGATGACAAGATCAGAACCGTGCAACG
CCGAACCGCTAAATGGAAGGCAGCTCATGGCAAGCCCAAAGAAGTGATGTTCAAAATTCAACATCATCCTGGGGAGATGGGACAGTCAGACTTCGCTCAG
CTCAAAGGTTTCAGTGTGACGGTTCAGGGTGAAGCCTTTCACCATATCTTGTATCACTATCGACTGAGCTATAGCGGCTGGCAGTACGTGCAGGTGATTC
AAGGCGGAGAAAGTTTTATCGGCTTATCCCAAGGGCTACAAAATGCTCTAACCGCCTGTGGTGGCGTGCCCAAGATTCATCGTACCGATAGTCTGAGTGC
GGCTTATCGCAATACTGGCGGCCGCAATCCCCAGCTGACCCAGTTGTATTCGACTCTGTGTGACCATTACCGGATGCAGCCAACACGCAACAATCTGGGT
GTCTCTCATGAGAATGGCGGAATAGAGGGGTCTCATGGCTATTTCAAACGACGCCTGTGCCAAGCCCTATATCGTCGAGGCAGCTTTGACTTCGAATCCG
TGGCCGCGTATCAGCAATTCATTGAGCAAGTCATCGCTAAGCTGAATGCCAAGTGCCAAAAAAAGTTTGCACTGGAGCTGCCCACCTTACAGCCCTTGCC
CAGATACCGAACTCCTGATTATGAAGTGCGCAGTGCCAAAGTCAGCTGCAATAGCACGATTGCCGTCCGCTGTGTCCTTTACACCGTCCCCTCTCGCTTA
ATTGGCCACCGCTTGAAATTACACCTATACCATGACCGCATTGTCGGTTTCTTAGGCACCACTCCCGTAGTGGAATTGGCGCGGGTCCATGTCCATGGCT
CTGAGAAGAAAAGACGCGCTCGCAGCATCGATTACAAGCATGTGGCAGAAAGTCTCAGGAGAAAGCCAAATGCATTTCTCTACTGCCAATGGCAATCAGA
GTTACTGCCCAATCTTCAGTGGCATCAGCTGTGGGAATCACTCAAAGCTAGCTTTGAGCGGGACCAGGCCGCACGATTAATCACCGAAGCCCTCTACATC
GCCGCGACCCAAGATCAAGAATCCCAGGTGGCCGACTACCTGCAAACCCAACTGGACCAGTCCACCCTGACTTTGAGCGGGTTAAAACAGGCCTTTGAGT
TCCAGCTTTCGACTGAGCAATACCCTGACGTCCCTTCACAGCAGCACAATTTATCTGACTATGACCAATTCCTCACCCTCGGATCTGGACAACCCATACC
AGTTGCTGATGACAACCCTCAAACAGCTCCGGTTAAGACATTTCCTCGACGAGTGGCAGAGCATCGAGCATCAAGCAACTCAGGAGAATTGGTCCTACGC
CCAGTTTCTATTGGCTTTAGCTCAAGGCGAAGCCAGCCGCCGAGAACAGAATCGGATCTCCCGCGCGCTGTCCGAAGCGCAACTTCCTTACGGAAAATCC
TGGACCAATTTTGAGTTTGACCATATTCCCAGTCTCAATCCAGCTGCTCTGATGGAGTTTTCCCAAACAACCCACTGGTTAGAGTCAGGTAGTAACATTC
TAATTTTTGGGCCGAGCGGAACAGGGAAAACACACGTCAGTTCAGCCTTGGGGACTTCCATGATTGAGTTGGGCAAGCGGGTAAAGTTTCTCACCGCCAC
AGCGCTGGTGCAACAACTGCAGCTGGCCAAACTTCAGCTGGAATTGCAAAGTCTATTATCCAAGCTCGACCGCTATGATCTGCTCATCATTGATGACCTA
GGCTATGTCCAAAAGTCCGATGTTGAAACCTCGGTCCTGTTTGAGCTGATTGCTCATCGCTATGAGCGAAAGAGTCTACTCATCACAGCCAATCAGCCTT
TCAGTCAATGGGACAAAATCTTTCCCAACTCGATGATGACCGTGGCTGCGATTGATCGCTTAATTCACCACGCCACCATTTTCGAGATGCAGGCAGAAAG
TTTCCGCAAACAAGAAGCTGCCAAACACACAGCCAAGTCTCAAAAGACTGACAGATAATCCCTTGCGCGCATAAACCCCCTTTTCCCCACCATTAATAGA
GTTGCTTATTTGTAAGCAGCCCTATTAATGGTGGCTTTATGGATAATTGTCATTTGTTCACCGGCGCGATCACCTACCGCAAAGATCATGATTTGTCGAT
TGCATGATGGTATCGCCAAGACCAAACTCGACTCACGAGCCATTATCAAAAGTATGAAGACAGAGCAAAAAAAGTTGGCCTATCTAATTGACATTAGACA
TCTGGCCA
GAGCATCAGTCACTTTCGATTTTTGATAGCCCCATACAGGAAAGACACTAGGGTTTGTACTCCTACGCAATTAGGAACCTCTAGTGCCAGCTCCTATTAA
ACCTCAACAGGTTCATCTTTATATGCAGACAAAGCAATCCGGCTCTTCTCAAGAAACAGCAGCTGCTAAAGCCGGCATTTCTACCCGCACAGCCCACCGC
ATTGATAGTGGTACTCATCGTCCCCAACGAGGCCGCCCTCATGACTGGAAAACCCGTGAAGATCCGCTGGATGGATTATGGGAATTAGAACTTGAGCCGA
TGTTAGAGCGTGAGCCTCGATTAGAAGCGACTACTCTATTTGAGACCTTACAAGAGAACCATCCTGGGCAATATGATGACAAGATCAGAACCGTGCAACG
CCGAACCGCTAAATGGAAGGCAGCTCATGGCAAGCCCAAAGAAGTGATGTTCAAAATTCAACATCATCCTGGGGAGATGGGACAGTCAGACTTCGCTCAG
CTCAAAGGTTTCAGTGTGACGGTTCAGGGTGAAGCCTTTCACCATATCTTGTATCACTATCGACTGAGCTATAGCGGCTGGCAGTACGTGCAGGTGATTC
AAGGCGGAGAAAGTTTTATCGGCTTATCCCAAGGGCTACAAAATGCTCTAACCGCCTGTGGTGGCGTGCCCAAGATTCATCGTACCGATAGTCTGAGTGC
GGCTTATCGCAATACTGGCGGCCGCAATCCCCAGCTGACCCAGTTGTATTCGACTCTGTGTGACCATTACCGGATGCAGCCAACACGCAACAATCTGGGT
GTCTCTCATGAGAATGGCGGAATAGAGGGGTCTCATGGCTATTTCAAACGACGCCTGTGCCAAGCCCTATATCGTCGAGGCAGCTTTGACTTCGAATCCG
TGGCCGCGTATCAGCAATTCATTGAGCAAGTCATCGCTAAGCTGAATGCCAAGTGCCAAAAAAAGTTTGCACTGGAGCTGCCCACCTTACAGCCCTTGCC
CAGATACCGAACTCCTGATTATGAAGTGCGCAGTGCCAAAGTCAGCTGCAATAGCACGATTGCCGTCCGCTGTGTCCTTTACACCGTCCCCTCTCGCTTA
ATTGGCCACCGCTTGAAATTACACCTATACCATGACCGCATTGTCGGTTTCTTAGGCACCACTCCCGTAGTGGAATTGGCGCGGGTCCATGTCCATGGCT
CTGAGAAGAAAAGACGCGCTCGCAGCATCGATTACAAGCATGTGGCAGAAAGTCTCAGGAGAAAGCCAAATGCATTTCTCTACTGCCAATGGCAATCAGA
GTTACTGCCCAATCTTCAGTGGCATCAGCTGTGGGAATCACTCAAAGCTAGCTTTGAGCGGGACCAGGCCGCACGATTAATCACCGAAGCCCTCTACATC
GCCGCGACCCAAGATCAAGAATCCCAGGTGGCCGACTACCTGCAAACCCAACTGGACCAGTCCACCCTGACTTTGAGCGGGTTAAAACAGGCCTTTGAGT
TCCAGCTTTCGACTGAGCAATACCCTGACGTCCCTTCACAGCAGCACAATTTATCTGACTATGACCAATTCCTCACCCTCGGATCTGGACAACCCATACC
AGTTGCTGATGACAACCCTCAAACAGCTCCGGTTAAGACATTTCCTCGACGAGTGGCAGAGCATCGAGCATCAAGCAACTCAGGAGAATTGGTCCTACGC
CCAGTTTCTATTGGCTTTAGCTCAAGGCGAAGCCAGCCGCCGAGAACAGAATCGGATCTCCCGCGCGCTGTCCGAAGCGCAACTTCCTTACGGAAAATCC
TGGACCAATTTTGAGTTTGACCATATTCCCAGTCTCAATCCAGCTGCTCTGATGGAGTTTTCCCAAACAACCCACTGGTTAGAGTCAGGTAGTAACATTC
TAATTTTTGGGCCGAGCGGAACAGGGAAAACACACGTCAGTTCAGCCTTGGGGACTTCCATGATTGAGTTGGGCAAGCGGGTAAAGTTTCTCACCGCCAC
AGCGCTGGTGCAACAACTGCAGCTGGCCAAACTTCAGCTGGAATTGCAAAGTCTATTATCCAAGCTCGACCGCTATGATCTGCTCATCATTGATGACCTA
GGCTATGTCCAAAAGTCCGATGTTGAAACCTCGGTCCTGTTTGAGCTGATTGCTCATCGCTATGAGCGAAAGAGTCTACTCATCACAGCCAATCAGCCTT
TCAGTCAATGGGACAAAATCTTTCCCAACTCGATGATGACCGTGGCTGCGATTGATCGCTTAATTCACCACGCCACCATTTTCGAGATGCAGGCAGAAAG
TTTCCGCAAACAAGAAGCTGCCAAACACACAGCCAAGTCTCAAAAGACTGACAGATAATCCCTTGCGCGCATAAACCCCCTTTTCCCCACCATTAATAGA
GTTGCTTATTTGTAAGCAGCCCTATTAATGGTGGCTTTATGGATAATTGTCATTTGTTCACCGGCGCGATCACCTACCGCAAAGATCATGATTTGTCGAT
TGCATGATGGTATCGCCAAGACCAAACTCGACTCACGAGCCATTATCAAAAGTATGAAGACAGAGCAAAAAAAGTTGGCCTATCTAATTGACATTAGACA
TCTGGCCA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1731 bp | 576 aa | 184 | 1914 | + | No |
Chemistry : DDE
ORF sequence :
GPAPIKPQQVHLYMQTKQSGSSQETAAAKAGISTRTAHRIDSGTHRPQRGRPHDWKTREDPLDGLWELELEPMLEREPRLEATTLFETLQENHPGQYDDK
IRTVQRRTAKWKAAHGKPKEVMFKIQHHPGEMGQSDFAQLKGFSVTVQGEAFHHILYHYRLSYSGWQYVQVIQGGESFIGLSQGLQNALTACGGVPKIHR
TDSLSAAYRNTGGRNPQLTQLYSTLCDHYRMQPTRNNLGVSHENGGIEGSHGYFKRRLCQALYRRGSFDFESVAAYQQFIEQVIAKLNAKCQKKFALELP
TLQPLPRYRTPDYEVRSAKVSCNSTIAVRCVLYTVPSRLIGHRLKLHLYHDRIVGFLGTTPVVELARVHVHGSEKKRRARSIDYKHVAESLRRKPNAFLY
CQWQSELLPNLQWHQLWESLKASFERDQAARLITEALYIAATQDQESQVADYLQTQLDQSTLTLSGLKQAFEFQLSTEQYPDVPSQQHNLSDYDQFLTLG
SGQPIPVADDNPQTAPVKTFPRRVAEHRASSNSGELVLRPVSIGFSSRRSQPPRTESDLPRAVRSATSLRKILDQF
IRTVQRRTAKWKAAHGKPKEVMFKIQHHPGEMGQSDFAQLKGFSVTVQGEAFHHILYHYRLSYSGWQYVQVIQGGESFIGLSQGLQNALTACGGVPKIHR
TDSLSAAYRNTGGRNPQLTQLYSTLCDHYRMQPTRNNLGVSHENGGIEGSHGYFKRRLCQALYRRGSFDFESVAAYQQFIEQVIAKLNAKCQKKFALELP
TLQPLPRYRTPDYEVRSAKVSCNSTIAVRCVLYTVPSRLIGHRLKLHLYHDRIVGFLGTTPVVELARVHVHGSEKKRRARSIDYKHVAESLRRKPNAFLY
CQWQSELLPNLQWHQLWESLKASFERDQAARLITEALYIAATQDQESQVADYLQTQLDQSTLTLSGLKQAFEFQLSTEQYPDVPSQQHNLSDYDQFLTLG
SGQPIPVADDNPQTAPVKTFPRRVAEHRASSNSGELVLRPVSIGFSSRRSQPPRTESDLPRAVRSATSLRKILDQF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 750 bp | 249 aa | 1709 | 2458 | + | No |
AG : IS21 helper
ORF sequence :
MTTLKQLRLRHFLDEWQSIEHQATQENWSYAQFLLALAQGEASRREQNRISRALSEAQLPYGKSWTNFEFDHIPSLNPAALMEFSQTTHWLESGSNILIF
GPSGTGKTHVSSALGTSMIELGKRVKFLTATALVQQLQLAKLQLELQSLLSKLDRYDLLIIDDLGYVQKSDVETSVLFELIAHRYERKSLLITANQPFSQ
WDKIFPNSMMTVAAIDRLIHHATIFEMQAESFRKQEAAKHTAKSQKTDR
GPSGTGKTHVSSALGTSMIELGKRVKFLTATALVQQLQLAKLQLELQSLLSKLDRYDLLIIDDLGYVQKSDVETSVLFELIAHRYERKSLLITANQPFSQ
WDKIFPNSMMTVAAIDRLIHHATIFEMQAESFRKQEAAKHTAKSQKTDR
Blast result :
Comments
ISAcma38 orfA and orfB are 67% and 78% aa similar to ISAcma26.
L1:TGACAATTACGTAGACAG (13-30)
R1:TGACAATTATCCATAAAG (2552-2535)
L1:TGACAATTACGTAGACAG (13-30)
R1:TGACAATTATCCATAAAG (2552-2535)
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)
2] ISfinder annotation (2009)