ISAar32
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1738 bp
Ends
IR Length : 15/19
IRL : CCCGGGTATTTCATGAAGGCCGGTAGTCATTAACTGACTTAAAAGATGAA
IRR : CCCAGATTTTTCATGAGGTCTCTGACGGCCACCGAAATCCGATACCCAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGAGCCGT | TTAA | ACGCAATACC | 4 |
DNA sequence
CCCGGGTATTTCATGAAGGCCGGTAGTCATTAACTGACTTAAAAGATGAAAAGGTGACCTGAACTGGGAAAATGGGAGTGTCTAATCTTCCAAAATACCC
GTTCAAAGGATCACCTTTTCAGTGAACCATTCTACCCACGTTTTCCCTGCTCTCTCGACCCAACTCACCGGCCAGTCCCTGGTCTCTCATGCAGGGTTGT
CGGTGCTGACCAGTTTCCTGAATGCCTTGGACTTCCGCAGCCTCTGCGAGGACCGGTTCAGCCAGTTCGTGCCAGCCACCGCTACCCACCGTCCAGGCAA
GATCCTCGGAGCACTGGCTCTATCGTTGGCGGCCGGCGGTGAACAAGCCACAGATATTGACCAGCTACGCACCGCACCAGAACTCTTCGGCTCCGTAGCG
TCGGATGCCACGGTCAGCCGATTCATGGGCCGGATCAAAGAACAGCCAGAAGCTTTCTCCTACGGGTTCGCCACCATGACCCGGCGCCTGCGCTCCAAAG
TCTGGAATGCGGCCGGGCCGCGGAATCCCGCCCGGCTGGCTACGGCAGCCAATCCGCTGATTATCGACATAGATGCGTCGCTAGTGCATGTCCATTCCGA
GAAAGAATCCAGCGCTGGGACGTATAAAGGCGGGTACGGTTTTTCACCGATGATCGCCATGGCCGACTACGGCAAAGCCCACGGCACCGGCGAAGTCCTC
GCCGTGCAATTGCGCCCGGGAAACCGGGGCGCGAACTCCGCCACCTCGCATATCGAGGTGCTCGGCCAAGCGCTGGCTCAGTTGCCTGATGATTTCTATG
ACGAGCACGGGAACCTTCGTGGTGAGAAGATCCTGGCCCGTACCGACAGTGCTGGGTCGTCCAGGGATTTTTTGCACCACCTGCATTCTCTGGGGCTCCA
ATTCTCCACGTCGTATTCGCTGCCGGTTCTCAAGGAGCGGTTCATCCGGTGGATCGATCAGAAGAAGTACTGGGAACCAGCCCTGGACGCTAACGGAGAT
CAGCGTGATGATGCATGGGTGATTGATGCAACGAACGTCTTGGAGTTGAGGCAATACCCGCCTGGAACTCGGATTTACCTGCGTGCTGAGCCCTTGCATC
CGGGGGCGAAAGCGAACTTGTTCGACACGGACGGGAACCGAGTCACCGCGTTCTTGACCAATGCGCCGCGGTTCAACGTCGCGTTCCTCGATGCCCGGCA
TCGGGCGCGGGGCAGGTGCGAAAACAGGATCAAGACGTTGAAGAATGCGGGGTTGGGCAAGCTGCCGTATTGGTCTTTCGCAGCGAACCAGGCGTGGGCG
GACTTGGCGATGTTCGCGGTGAATTTGGTGTCTTGGCTTCAACTTGCTGCGCTACCTGGCGGGCATGAGGCCGGCTGCTGGGATTTGAAGCGGTGGCGGT
ACCGGCTATTTTCAATGGCCGGGAAAGTCGTCACCGGCGGCCGGCAACGCCGGCTGCTGATCGCCGAGAAGGCGCCTGAAGCGCAGCTGTTATGCCAGTT
GCAGAAGGGTATCGGTGAGTCATTCCAGCGGTGGCGGCACGGCGAGCTGGCCGCCTGAACTGGTGATTGTTCTGTTGTTTATTTGGTTTCGAAAGGCCAA
TTCAGTCCAGATAAGTGGAGCCTGGCACCCAGCGCTGGTGTTTCGGCGCTCTTTGGCGTGGGCGGAATCAGTAACGGCCGCCGGGAATCCTGGGTATCGG
ATTTCGGTGGCCGTCAGAGACCTCATGAAAAATCTGGG
GTTCAAAGGATCACCTTTTCAGTGAACCATTCTACCCACGTTTTCCCTGCTCTCTCGACCCAACTCACCGGCCAGTCCCTGGTCTCTCATGCAGGGTTGT
CGGTGCTGACCAGTTTCCTGAATGCCTTGGACTTCCGCAGCCTCTGCGAGGACCGGTTCAGCCAGTTCGTGCCAGCCACCGCTACCCACCGTCCAGGCAA
GATCCTCGGAGCACTGGCTCTATCGTTGGCGGCCGGCGGTGAACAAGCCACAGATATTGACCAGCTACGCACCGCACCAGAACTCTTCGGCTCCGTAGCG
TCGGATGCCACGGTCAGCCGATTCATGGGCCGGATCAAAGAACAGCCAGAAGCTTTCTCCTACGGGTTCGCCACCATGACCCGGCGCCTGCGCTCCAAAG
TCTGGAATGCGGCCGGGCCGCGGAATCCCGCCCGGCTGGCTACGGCAGCCAATCCGCTGATTATCGACATAGATGCGTCGCTAGTGCATGTCCATTCCGA
GAAAGAATCCAGCGCTGGGACGTATAAAGGCGGGTACGGTTTTTCACCGATGATCGCCATGGCCGACTACGGCAAAGCCCACGGCACCGGCGAAGTCCTC
GCCGTGCAATTGCGCCCGGGAAACCGGGGCGCGAACTCCGCCACCTCGCATATCGAGGTGCTCGGCCAAGCGCTGGCTCAGTTGCCTGATGATTTCTATG
ACGAGCACGGGAACCTTCGTGGTGAGAAGATCCTGGCCCGTACCGACAGTGCTGGGTCGTCCAGGGATTTTTTGCACCACCTGCATTCTCTGGGGCTCCA
ATTCTCCACGTCGTATTCGCTGCCGGTTCTCAAGGAGCGGTTCATCCGGTGGATCGATCAGAAGAAGTACTGGGAACCAGCCCTGGACGCTAACGGAGAT
CAGCGTGATGATGCATGGGTGATTGATGCAACGAACGTCTTGGAGTTGAGGCAATACCCGCCTGGAACTCGGATTTACCTGCGTGCTGAGCCCTTGCATC
CGGGGGCGAAAGCGAACTTGTTCGACACGGACGGGAACCGAGTCACCGCGTTCTTGACCAATGCGCCGCGGTTCAACGTCGCGTTCCTCGATGCCCGGCA
TCGGGCGCGGGGCAGGTGCGAAAACAGGATCAAGACGTTGAAGAATGCGGGGTTGGGCAAGCTGCCGTATTGGTCTTTCGCAGCGAACCAGGCGTGGGCG
GACTTGGCGATGTTCGCGGTGAATTTGGTGTCTTGGCTTCAACTTGCTGCGCTACCTGGCGGGCATGAGGCCGGCTGCTGGGATTTGAAGCGGTGGCGGT
ACCGGCTATTTTCAATGGCCGGGAAAGTCGTCACCGGCGGCCGGCAACGCCGGCTGCTGATCGCCGAGAAGGCGCCTGAAGCGCAGCTGTTATGCCAGTT
GCAGAAGGGTATCGGTGAGTCATTCCAGCGGTGGCGGCACGGCGAGCTGGCCGCCTGAACTGGTGATTGTTCTGTTGTTTATTTGGTTTCGAAAGGCCAA
TTCAGTCCAGATAAGTGGAGCCTGGCACCCAGCGCTGGTGTTTCGGCGCTCTTTGGCGTGGGCGGAATCAGTAACGGCCGCCGGGAATCCTGGGTATCGG
ATTTCGGTGGCCGTCAGAGACCTCATGAAAAATCTGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1437 bp | 478 aa | 122 | 1558 | + | No |
Chemistry : DDE
ORF sequence :
VNHSTHVFPALSTQLTGQSLVSHAGLSVLTSFLNALDFRSLCEDRFSQFVPATATHRPGKILGALALSLAAGGEQATDIDQLRTAPELFGSVASDATVSR
FMGRIKEQPEAFSYGFATMTRRLRSKVWNAAGPRNPARLATAANPLIIDIDASLVHVHSEKESSAGTYKGGYGFSPMIAMADYGKAHGTGEVLAVQLRPG
NRGANSATSHIEVLGQALAQLPDDFYDEHGNLRGEKILARTDSAGSSRDFLHHLHSLGLQFSTSYSLPVLKERFIRWIDQKKYWEPALDANGDQRDDAWV
IDATNVLELRQYPPGTRIYLRAEPLHPGAKANLFDTDGNRVTAFLTNAPRFNVAFLDARHRARGRCENRIKTLKNAGLGKLPYWSFAANQAWADLAMFAV
NLVSWLQLAALPGGHEAGCWDLKRWRYRLFSMAGKVVTGGRQRRLLIAEKAPEAQLLCQLQKGIGESFQRWRHGELAA
FMGRIKEQPEAFSYGFATMTRRLRSKVWNAAGPRNPARLATAANPLIIDIDASLVHVHSEKESSAGTYKGGYGFSPMIAMADYGKAHGTGEVLAVQLRPG
NRGANSATSHIEVLGQALAQLPDDFYDEHGNLRGEKILARTDSAGSSRDFLHHLHSLGLQFSTSYSLPVLKERFIRWIDQKKYWEPALDANGDQRDDAWV
IDATNVLELRQYPPGTRIYLRAEPLHPGAKANLFDTDGNRVTAFLTNAPRFNVAFLDARHRARGRCENRIKTLKNAGLGKLPYWSFAANQAWADLAMFAV
NLVSWLQLAALPGGHEAGCWDLKRWRYRLFSMAGKVVTGGRQRRLLIAEKAPEAQLLCQLQKGIGESFQRWRHGELAA
Blast result :
Comments
ISAar32 is 95% aa similar to ISAar8.
ISAar32 was reconstructed in silico by deletion of 2 ISAar14 sequences (an ISAar14 inserted inside another ISAar14) and one of the direct repeat generated by its insertion.
ISAar32 was reconstructed in silico by deletion of 2 ISAar14 sequences (an ISAar14 inserted inside another ISAar14) and one of the direct repeat generated by its insertion.
References
1] ISfinder annotation (2009)