ISEc30
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011603 | ND | Escherichia coli | Escherichia coli B171 plasmid pB171 Escherichia coli O127:H6 E2348/69 plasmid pMAR2 Escherichia coli plasmid pMAR7 |
DNA section
IS Length : 766 bp
Ends
IR Length : 20/27
IRL : GGTGATGCCTCTAATTAGTTGAATCTGATGTATAATACGGGTTTTTGAGG
IRR : GGTAGTGCATCCAATTAGTAGAACATGTGTTTTTCGATAAACGCTCCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGCTGGATA | CAGTGTGTAA | 0 | |
| ATGCTGGATA | CAGTGTGTAA | 0 | |
| ATGCTGGATA | CAGTGTGTAA | 0 |
DNA sequence
GGTGATGCCTCTAATTAGTTGAATCTGATGTATAATACGGGTTTTTGAGGTTATCTCATGGCCAGAGTTAATATTCATTGTCCCCGTTGTCAGTCAGCTC
AGGTTTACCGCCATGGTCAGAACCCTAAAGGCCGTGACAGACTTCGCTGCCGTGACTGCCACCGTGTGTTTCAGCTCACTTATACTTATCAAGCACGTAA
GCCGGGTATGAAAGAGCTGATCACTGAAATGGCCTTTAATGGTGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGTAGTAACACCGTCATCCGG
ACTTTAAAAAACTCGCGCCAAAGCGAATAACTTCTTCGCCTGTTACCCATGCTGATGTGGCGTTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGG
CAGTAAAGCCCGGCAACACTGGATCTGGTACGCGTACAACACCAAAACAGGCGGTGTACTGGCCTACACTTTTGGTCCCCGAACCGATCAAACGTGCCGG
GAGCTACTGGCACTGCTTACACCCTTCAACATCGGCATGCTGACCAGCGATGACTGGGGCAGCTATGGCCGGGAGGTGCCGAAGAATAAGCATCTGACCG
GCAAAATATTCACCCAATGCATTGAGCGTAATAATCTGACGCTACGCACCCGCATTAAGCGGTTGGGTCGTAAAACAATCTGCTTCTCACGCTCAGTTGA
GATCCACGAAAAAGTGATCGGAGCGTTTATCGAAAAACACATGTTCTACTAATTGGATGCACTACC
AGGTTTACCGCCATGGTCAGAACCCTAAAGGCCGTGACAGACTTCGCTGCCGTGACTGCCACCGTGTGTTTCAGCTCACTTATACTTATCAAGCACGTAA
GCCGGGTATGAAAGAGCTGATCACTGAAATGGCCTTTAATGGTGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGTAGTAACACCGTCATCCGG
ACTTTAAAAAACTCGCGCCAAAGCGAATAACTTCTTCGCCTGTTACCCATGCTGATGTGGCGTTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGG
CAGTAAAGCCCGGCAACACTGGATCTGGTACGCGTACAACACCAAAACAGGCGGTGTACTGGCCTACACTTTTGGTCCCCGAACCGATCAAACGTGCCGG
GAGCTACTGGCACTGCTTACACCCTTCAACATCGGCATGCTGACCAGCGATGACTGGGGCAGCTATGGCCGGGAGGTGCCGAAGAATAAGCATCTGACCG
GCAAAATATTCACCCAATGCATTGAGCGTAATAATCTGACGCTACGCACCCGCATTAAGCGGTTGGGTCGTAAAACAATCTGCTTCTCACGCTCAGTTGA
GATCCACGAAAAAGTGATCGGAGCGTTTATCGAAAAACACATGTTCTACTAATTGGATGCACTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 273 bp | 90 aa | 58 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MARVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQLTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTLKNSRQSE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 154 aa | 288 | 752 | + | No |
Description : Second part of the transposase
ORF sequence :
HRHPDFKKLAPKRITSSPVTHADVAFICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKN
KHLTGKIFTQCIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEKHMFY
KHLTGKIFTQCIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEKHMFY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 695 bp | 231 aa | 58 | 752 | + | Yes |
Chemistry : DDE
ORF sequence :
MARVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQLTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTLKKLAPKRITSSPVTHAD
VAFICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKNKHLTGKIFTQCIERNNLTLRTRI
KRLGRKTICFSRSVEIHEKVIGAFIEKHMFY
VAFICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKNKHLTGKIFTQCIERNNLTLRTRI
KRLGRKTICFSRSVEIHEKVIGAFIEKHMFY
Blast result :
Comments
ISEc30 is 95% aa similar to IS1N, and 90% aa similar to IS1H.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Viscardi,M. (2009) Direct submission.
2] Thomson,N.R. (2008) Direct submission GenBank.
2] Thomson,N.R. (2008) Direct submission GenBank.