ISAar28

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FQ311875 ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1593 bp

Ends


IR Length : 41/50

IRL : TGTTTTCTCTCAGGACATCGGTAACAGTTCTGCCTCAGGACATCGGTAAC
IRR : TGTGATGTCTCAAGACACCGGTGACACTTCTGTCCCAGGACATCGGTAAC

Insertion site


Left flankDirect repeatRight flankDR Length
CAAATTTCAATCGTTAAATTAATTGAAGTTTGACT15
TCGTGCGCCGATTTCCTTTTAAAGATCACATAGGC15
TATTGTCCCACTTTTAAACAGAAGCTGGTGGTGTC15

DNA sequence

TGTTTTCTCTCAGGACATCGGTAACAGTTCTGCCTCAGGACATCGGTAACACTTCAACATTTAAAGTGGTGACACTTCTCCCCGGGATTTAACGCCCCGA
AGGACACAGCCCGCCTACCGGCCTCTCGGCCAGCACGCGGGCTGATGAACTGGAAAGTGTGAAAAATGATCCAGTCAACTCGACAATACGCTTAGCCATC
GCCCGATGGCCACAAGACGCTCCCAGAGGCGCGGTCACCGCCTTCTGCACGGAACACGAAATTAGCCGCAAGACTTTCTACCAAATCCGCAAACGAGCCC
AAACCGAAGGCCAAGCAGCAGCCCTGGAACCCCGCTCCCGACGGCCGAAAAAAGCGCCTACCGCAACAAACGACGCCGTCAAGGACCAAGCGGTCGCAAT
GCGCAAGGCGTTGCAAGCCTCGGGCTGGGATCACGGACCGATCAGCGTGCACGATCACATGAAGAAGCTGGGCATGCCCACGGTATCGATCTCGGCACTG
GCCCGGATCTTCTCCGAACGAGGCCTGGTTACCCCAGCCCCGCAGAAGCGTCCGCGTTCCTCCTACCGGTCCTTCCGCTATCCGATGCCCAATTCCTGCT
GGCAGCTGGACGCCACCGAATACGTGCTGGAAGGAGGACGCAAATGCGTGATCTTCCAGCTGATCGATGACCATTCCCGGCTGGCGGTCGCTTCATTGGC
CGCCAAGACCGAGAACGGCCAAGATGCAGTGAAGGTCTTCCGCAAAGGCATCGAATCGTGCGGCGTGCCGCAAAGACTGCTCACCGATAATGGTGATGCG
CTGAACCCCATCCGCCGTGGCGTGCTGAGCGAGCTGGTCGCTTACGCCAACGGTCTGGGCATCGCGACGATCACTGGCAAACCCCACAAGCCCACCACCC
AGGGCAAGAACGAACGGCTTCATTCAACCTTGTTCAAATGGCTGAAAAAGCAGCCGTTCGCCACCGATCTAGCACAGTTGCAGGCGCAGCTGGATGTCTT
CGACCATGCCTATAACACCCAACGCGGGCACCAGTCCCTGGAAGATCGGATGACTCCGCAAGAAGCGTGGGATGCAACGCCCGCAGCTGAGCCGCTGGAG
CTTGGGCAATGGAAGGAACTGAGCACGAAACCAGGACCGAGCCAGGCTCAAGTAGCTCTTGGCGTCCAAGCCGGAAGTGAGCGCGTGGCCCGGCAGCTGG
CTGCTGCACGAACCCCTCCAGAGCCGGCTAAGCTCAGCGCCACGCTGCGTTCTGAACTCATGGGCGAGAGCGGAAGTCACGTGCTGCGAGCCAATAAGAA
CCGCTGCCTGCGGGTTGCCGGGGTTGAAATTTATCTGGGCAAGCTACTGCGCTCCACAGTGGTAAATGTGGTTTGGGACCCGACAGACTTGATGGTGCTG
AGCATGGAGGGTGAACTGGTCGCGAAATTCGATTACCCGTTCCCGCAGGGCGTGAAGTATTTGAGTTTGAAGCATGCCACGGAGAAGTTCCAAAACATGC
CTGACCCGGTCTGATCTGTTACCGATGTCCTGAGACACCGAGTGTTACCGATGTCCTGGGACAGAAGTGTCACCGGTGTCTTGAGACATCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1356 bp451 aa1591514+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VKNDPVNSTIRLAIARWPQDAPRGAVTAFCTEHEISRKTFYQIRKRAQTEGQAAALEPRSRRPKKAPTATNDAVKDQAVAMRKALQASGWDHGPISVHDH
MKKLGMPTVSISALARIFSERGLVTPAPQKRPRSSYRSFRYPMPNSCWQLDATEYVLEGGRKCVIFQLIDDHSRLAVASLAAKTENGQDAVKVFRKGIES
CGVPQRLLTDNGDALNPIRRGVLSELVAYANGLGIATITGKPHKPTTQGKNERLHSTLFKWLKKQPFATDLAQLQAQLDVFDHAYNTQRGHQSLEDRMTP
QEAWDATPAAEPLELGQWKELSTKPGPSQAQVALGVQAGSERVARQLAAARTPPEPAKLSATLRSELMGESGSHVLRANKNRCLRVAGVEIYLGKLLRST
VVNVVWDPTDLMVLSMEGELVAKFDYPFPQGVKYLSLKHATEKFQNMPDPV

 

Blast result :
Comments
ISAar28 is 71% aa similar to ISArsp2.
References
1] ISfinder annotation (2009)