ISAar27

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1669 bp

Ends


IR Length : 36/50

IRL : TGTCCTGTCTCAAGACTTTGTGGACACACGATCTCATGACATCGTGGACA
IRR : TGTGAAATTTCGTGACTTCGTGGACACTGAATCTCGTCACTTCGTGGACA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTGGTGCTACCCGATACGCGCACATACGCTACC14

DNA sequence

TGTCCTGTCTCAAGACTTTGTGGACACACGATCTCATGACATCGTGGACACGATGTCTCATACCTTCGTGGACATTCGAGACGTGATTTTCTGGCACGCT
CAATCGCATGCCTAACGCACTCTCTCCACGCCTGCGCGCGATGATCATCAGTTTCGATCCCACACAGCCCGAGGCCCTGAGCATCAGCGAATTCTGCAAG
ACCCAGAAAATCTCGCGAAGCATCTTCTACCGCATCCGCGAACGAGCCACACAAGAATCAGCCGGCGCGCTCCACCCGCGCTCCAGAGCCCCGAAACTAC
CCTCCAGACGCTACGGTCCCGACGTGATCAATGAGCTGGTCAGGATCCGCAAGGAACTCAAAAAGGATGGGTGGGACTACGGACCGAAGACCATCCATTA
CGAAGCGACCATCCTGGACGAGTTTCCTGGTGGCCAGATCCCGTCCGTGGCGACCATTGCGCGGCTGCTGGCCAGTGTGGGGCATGTTGAGCGCAGTCCG
CGCAAGCGCCCGAAATCCTCGTACGTTCCCTTCGCGCGCTCTGCTGCCATGGCGTTGTGGCAACTCGACGCGTTCGAGTTCAGAACCCATTCCGATCAGG
TGGTGACCGTCTACCAGCTCATTGACGATGCGACCCGATTCGACGTGGGCTCCAGCGCGTACGCACGCCATGAGAACAGCGGTGACGCGCAACAGGTGCT
CGCACGTGCCATCAATGACTACGGGCCGCCCAAGGAAGTCCTCAGCGATAACTCCAAGGCGTTCAATCAGCTTCGCGGCGGAACCATCGGCATCGTGGAA
GCCTATCTGGCGTCCCAGGGAACAATGCCGATCACGGGGTTGCCGGGCAGGCCGACGACTCAGGGCAAGAATGAACGTTCGCACCAGACCCTTCAGCAGT
TCCTCAAGGCCAACAGGCCGCAAAATCTTGCGGACGTGCAGAAGCTGCTTCGACGTTATCGTGAGCATTATAACCAGCGCCGGCCCCATCAATCCTTGAA
CCAGGCAACCCCGCAGAAGGCCTGGGAGCTGCTGGAGCACACGCCGGCAACCGAGCCGATCTCGATGGTGGTGCTGGAAGCGAAGGCCGCTGAATACCTG
ATGAAGCGTCGAATCGGAAGCTCGGCGGCGAATCGCGCGGATGTTGTCGTTTCGAAAACTGGCGACATCCTGAAGAAGCTCACGGAGCAGCACGAGCCTG
ACATGGATCGGGAAAGCCATCAGTTACTAGTGCGGGTCAATAAGGATAATTGCCAAGCCTATTATCGTGGCAAGCAGATCTCCCTGCCGCAGACCTATGC
TGGACGCCAGTTCCTGAGGACCATCACCGAAGACGAATTCATCTTGTCGGATCCCGATACCGCGGAAGTGGTGCTGAGTTTCCCGTTGCCCATGGTGGCG
TTGCGTGTTCATCGGCGGTTTGTGTCCTCGTACTCGATCAGGGGAATTCGCTTGGCGAACCCGACAAAGCAGTGGAGCCGGAAGGTCGCCGAGTACCAGG
CCCAGTACGAGGCGCGGGAAGAAGACATGCCCGAGGTCTTTGATTATCGGTAGTTGGCTGTTGCCGGCGGCGCTCGAAGGGGAGCCGCCGGTAGCTGTGT
ACAAAGTATTGAGATCCATTGTCCACGAAGTGACGAGATTCAGTGTCCACGAAGTCACGAAATTTCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1446 bp481 aa1081553+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPNALSPRLRAMIISFDPTQPEALSISEFCKTQKISRSIFYRIRERATQESAGALHPRSRAPKLPSRRYGPDVINELVRIRKELKKDGWDYGPKTIHYEA
TILDEFPGGQIPSVATIARLLASVGHVERSPRKRPKSSYVPFARSAAMALWQLDAFEFRTHSDQVVTVYQLIDDATRFDVGSSAYARHENSGDAQQVLAR
AINDYGPPKEVLSDNSKAFNQLRGGTIGIVEAYLASQGTMPITGLPGRPTTQGKNERSHQTLQQFLKANRPQNLADVQKLLRRYREHYNQRRPHQSLNQA
TPQKAWELLEHTPATEPISMVVLEAKAAEYLMKRRIGSSAANRADVVVSKTGDILKKLTEQHEPDMDRESHQLLVRVNKDNCQAYYRGKQISLPQTYAGR
QFLRTITEDEFILSDPDTAEVVLSFPLPMVALRVHRRFVSSYSIRGIRLANPTKQWSRKVAEYQAQYEAREEDMPEVFDYR

 

Blast result :
Comments
ISAar27 is 57% aa similar to ISAar22.
References
1] ISfinder annotation (2009)