ISAar26
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1344 bp
Ends
IR Length : 20/26
IRL : TGGGGTTAGCCTGAATTAGTGGACACCCGAACAAGCGGGATCAGCCGATC
IRR : TGAGGTTCCCCGCAACTAGTGGACACGTGATCTAGCTCATGCTGCTAGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAGAGGCCG | a/cAAC | TGAGGAAAAT | 6 |
DNA sequence
TGGGGTTAGCCTGAATTAGTGGACACCCGAACAAGCGGGATCAGCCGATCCCGTATTTGAGAGGATGTCATTAATGAGCAATATGAAAAAGCAACGCAGG
TCCTTCACCGAGGAATACCGCCGGGAAGCAGCCGGCCTGGTCATCGACACCGGACGCAGCATCAGCGCAGTCGCCAAGGAACTGAACCTCGGCGAGCAGA
CACTGGGCACCTGGGTGAAGAAGGAACGATCCCGCCGAGGCCTGGACGGCGAGCCGACCGGAGCGCTGGACGAGTCCGAACGCGAAGAACTCAAACGCTT
GCGCAAGGAAGTCTTCGAGCTGCGGGAAGACAACCGCTTCCTGGGAAAAGCGGCCTCCTTCTTCGCGTCGAAGCAACATCGGTCGAACGGTTCGAACTGA
TGCACGCGTTGAAGGATGAGTTCGCCTTGACCCGCATGGCGGCCTTGCTTCACGTCTCGAAATCCGGGTACTACGCCTGGAAGCAACGCCAAACCGCTGG
CCCATCCGCAAGGGCTGCCGCGCAACGGGAGCGAGATGCGAAGGTCGCGAAGGTCTTCAACGACTCCCACCAGACCTACGGAGCCCCTCGGGTGGCCGCC
CAGCTGGCCCGCGAAGGATCACCAGCGGATCCCAAGACCGTGGCTGCCTCGATGCTGCGCCAAGGCATCGAAGGGATCAGCCCCAGGAGGTTCACCCCCG
TGACCACGATCCAAGGCGTTGACACCTACCACCTGCCCGATCGCGTGCACCGCCAATGGGACCAGGGCGCCTTGGACAAGGTCTGGATCAGCGACATCAC
CTACCTGCGTACCAATGAGGGCTGGCTGTACTTGTGCGCTGTGCGTGATGGCTGCTCCCGCCGGGTGCTGGGCTGGTCGCTGGACAGCGTGCAGAATACC
GATTTGGTGGAAAGGGCCCTGCGCATGGCCAGGACCCTGCGGGGGAAGATTCCGGGTCAGGTGGTGTTCCACGCGGACCGCGGCTCGCAGTTCACTTCCG
CCCAGCTGCATGAGGTGGCTGTTGAGCTGGATCTGCTGCAGTCCGTGGGGCGCACGGGCGTGTGCTGGGATAACGCCATGAGCGAGTCATTCTGGTCGAC
GCTGAAGACCGAGTTCTATGACCGGTACCGGTGGGCTACCCGGGCGGACGCCAAGCAGAAGGTGGCTTGGTGGATCGAGGATTTCTATAACCGCCGCCGG
TTGCATTCAAGTCTGGGGATGGTTCCTCCGGTGGAGTTTGAGCAGAAGCTTCGCGGTCAGCAGGAACGCGTTGAACAGCATAGGACCGTGCTAACGCTAG
CAGCATGAGCTAGATCACGTGTCCACTAGTTGCGGGGAACCTCA
TCCTTCACCGAGGAATACCGCCGGGAAGCAGCCGGCCTGGTCATCGACACCGGACGCAGCATCAGCGCAGTCGCCAAGGAACTGAACCTCGGCGAGCAGA
CACTGGGCACCTGGGTGAAGAAGGAACGATCCCGCCGAGGCCTGGACGGCGAGCCGACCGGAGCGCTGGACGAGTCCGAACGCGAAGAACTCAAACGCTT
GCGCAAGGAAGTCTTCGAGCTGCGGGAAGACAACCGCTTCCTGGGAAAAGCGGCCTCCTTCTTCGCGTCGAAGCAACATCGGTCGAACGGTTCGAACTGA
TGCACGCGTTGAAGGATGAGTTCGCCTTGACCCGCATGGCGGCCTTGCTTCACGTCTCGAAATCCGGGTACTACGCCTGGAAGCAACGCCAAACCGCTGG
CCCATCCGCAAGGGCTGCCGCGCAACGGGAGCGAGATGCGAAGGTCGCGAAGGTCTTCAACGACTCCCACCAGACCTACGGAGCCCCTCGGGTGGCCGCC
CAGCTGGCCCGCGAAGGATCACCAGCGGATCCCAAGACCGTGGCTGCCTCGATGCTGCGCCAAGGCATCGAAGGGATCAGCCCCAGGAGGTTCACCCCCG
TGACCACGATCCAAGGCGTTGACACCTACCACCTGCCCGATCGCGTGCACCGCCAATGGGACCAGGGCGCCTTGGACAAGGTCTGGATCAGCGACATCAC
CTACCTGCGTACCAATGAGGGCTGGCTGTACTTGTGCGCTGTGCGTGATGGCTGCTCCCGCCGGGTGCTGGGCTGGTCGCTGGACAGCGTGCAGAATACC
GATTTGGTGGAAAGGGCCCTGCGCATGGCCAGGACCCTGCGGGGGAAGATTCCGGGTCAGGTGGTGTTCCACGCGGACCGCGGCTCGCAGTTCACTTCCG
CCCAGCTGCATGAGGTGGCTGTTGAGCTGGATCTGCTGCAGTCCGTGGGGCGCACGGGCGTGTGCTGGGATAACGCCATGAGCGAGTCATTCTGGTCGAC
GCTGAAGACCGAGTTCTATGACCGGTACCGGTGGGCTACCCGGGCGGACGCCAAGCAGAAGGTGGCTTGGTGGATCGAGGATTTCTATAACCGCCGCCGG
TTGCATTCAAGTCTGGGGATGGTTCCTCCGGTGGAGTTTGAGCAGAAGCTTCGCGGTCAGCAGGAACGCGTTGAACAGCATAGGACCGTGCTAACGCTAG
CAGCATGAGCTAGATCACGTGTCCACTAGTTGCGGGGAACCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 74 | 400 | + | No |
Description : First part of the transposase
ORF sequence :
MSNMKKQRRSFTEEYRREAAGLVIDTGRSISAVAKELNLGEQTLGTWVKKERSRRGLDGEPTGALDESEREELKRLRKEVFELREDNRFLGKAASFFASK
QHRSNGSN
QHRSNGSN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1008 bp | 335 aa | 301 | 1308 | + | No |
Description : Second part of the transposase
ORF sequence :
AQGSLRAAGRQPLPGKSGLLLRVEATSVERFELMHALKDEFALTRMAALLHVSKSGYYAWKQRQTAGPSARAAAQRERDAKVAKVFNDSHQTYGAPRVAA
QLAREGSPADPKTVAASMLRQGIEGISPRRFTPVTTIQGVDTYHLPDRVHRQWDQGALDKVWISDITYLRTNEGWLYLCAVRDGCSRRVLGWSLDSVQNT
DLVERALRMARTLRGKIPGQVVFHADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRR
LHSSLGMVPPVEFEQKLRGQQERVEQHRTVLTLAA
QLAREGSPADPKTVAASMLRQGIEGISPRRFTPVTTIQGVDTYHLPDRVHRQWDQGALDKVWISDITYLRTNEGWLYLCAVRDGCSRRVLGWSLDSVQNT
DLVERALRMARTLRGKIPGQVVFHADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRR
LHSSLGMVPPVEFEQKLRGQQERVEQHRTVLTLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1235 bp | 411 aa | 74 | 1308 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNMKKQRRSFTEEYRREAAGLVIDTGRSISAVAKELNLGEQTLGTWVKKERSRRGLDGEPTGALDESEREELKRLRKEVFELREDNRFLGKSGLLLRVE
ATSVERFELMHALKDEFALTRMAALLHVSKSGYYAWKQRQTAGPSARAAAQRERDAKVAKVFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIE
GISPRRFTPVTTIQGVDTYHLPDRVHRQWDQGALDKVWISDITYLRTNEGWLYLCAVRDGCSRRVLGWSLDSVQNTDLVERALRMARTLRGKIPGQVVFH
ADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRRLHSSLGMVPPVEFEQKLRGQQERV
EQHRTVLTLAA
ATSVERFELMHALKDEFALTRMAALLHVSKSGYYAWKQRQTAGPSARAAAQRERDAKVAKVFNDSHQTYGAPRVAAQLAREGSPADPKTVAASMLRQGIE
GISPRRFTPVTTIQGVDTYHLPDRVHRQWDQGALDKVWISDITYLRTNEGWLYLCAVRDGCSRRVLGWSLDSVQNTDLVERALRMARTLRGKIPGQVVFH
ADRGSQFTSAQLHEVAVELDLLQSVGRTGVCWDNAMSESFWSTLKTEFYDRYRWATRADAKQKVAWWIEDFYNRRRLHSSLGMVPPVEFEQKLRGQQERV
EQHRTVLTLAA
Blast result :
Comments
ISAar26 is 54% aa similar to ISFsp8. The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).