ISAar25
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 19/25
IRL : TGAGCTGTCATGATGTTGTGAGACACGCGTTCGTAGGAGAATGAGTGCAT
IRR : TGAGCTGGTCAAGTTCTTGAGACACGATCTTTGATCTTTTCTAAATATTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGTGAATTC | AGG | AGAGTATGTG | 3 |
DNA sequence
TGAGCTGTCATGATGTTGTGAGACACGCGTTCGTAGGAGAATGAGTGCATGTCCAAACGTCAGAAGTTCACTCCGGAGTTCCGTGCCGAAGCAGTCGAAC
TAGTGATCTCCTCCCAGAAGAGCCTGGTCGAGGTCGCAGCCTCTCTAGGCATCAACGAAGGAACCCTGGGCAACTGGGTCCGCGCTTACCGCACCGAGCA
CCCCGAAGCGGAAACCGAGGAACGCGGACCGGTGGAATGGGCCCAATACGAAAAGCTACGCAAGGAACTGCTGGAGGTGAAACGGGAGAACGACTTTTTA
AAAGATGTCAGCGCCTATTTCGCCTCGAACAGCAAGAAATAAGCGCAATCTTCACCTTCATCAAAGCCCATGCCGGCCAGTACCCGGTGAACTGGATGTG
CCAGAAACTGCAGGTTTCCAGGGCGTCCTATTACCGGTGGCTAAACCCCTGCGAGGAGCTGAGCCAAGCCCAAGCACTGCGCAGGCGCCGCACCGAACTG
GTCCACACCGAATTCGAAGCATCCAACCAGGTTGCTGGGGCAGGTCAACTGGTGGACCTGCTCCGCAACAACCATGAGGAACAGATCCCGAAGTCTACGG
TCCTCTCGATCATGAAAGACCTGGGGATCCAAGCCAAACGGATGAGCGCGTTCAAGACCACGACGGTTCGTGATCATCAGGCCCGGACGGCGCATATCAA
GAACCATATGGTCGATAAGCTCGGCCGGCGGGACTTCCGCTCTTGCCGTCCTGGGGCGAAACTGGTTGGCGATATCACCTACCTGCGCACCGGCGAAGGC
TGGTTGTATTTGGCGACGGTCATCGATTTGTATTCGCGTCAGATCGTGGGCTGGTCAATGGGCGAGAACATGCAAACTCAGCTGGTCATTGACGCGATGG
AGCTTGCCGCGTCGCGGGGCTTTATCCAGCCTGGCACCGTGTTCCATTCAGACCGGGGCACGCAGTACACCTCTGGCGCGTTCCAGGCATGGTGCGCTGA
GCAAGGGGTGCGGCAGTCGATGGGGCGCACGGGGGTGTGCTGGGACAACTCCGTCGCAGAGTCAACCTTCTCGACGATCAAGAACGAAATGTATCACCAC
CGGGCTTTCACCAGCAGAGTAGCAGCGCGTACCGCGGTGATGGAATACATCGAGGTTTGGTACAACCGGAAGCGACCGCATACAGCTAACGGAGGGCTCA
GCCCTCAGGCAGTTCTGGACTCGTACCAGGAAAGAAATTCTTTAGCCTTGGCGGCTTGAGAATATTTAGAAAAGATCAAAGATCGTGTCTCAAGAACTTG
ACCAGCTCA
TAGTGATCTCCTCCCAGAAGAGCCTGGTCGAGGTCGCAGCCTCTCTAGGCATCAACGAAGGAACCCTGGGCAACTGGGTCCGCGCTTACCGCACCGAGCA
CCCCGAAGCGGAAACCGAGGAACGCGGACCGGTGGAATGGGCCCAATACGAAAAGCTACGCAAGGAACTGCTGGAGGTGAAACGGGAGAACGACTTTTTA
AAAGATGTCAGCGCCTATTTCGCCTCGAACAGCAAGAAATAAGCGCAATCTTCACCTTCATCAAAGCCCATGCCGGCCAGTACCCGGTGAACTGGATGTG
CCAGAAACTGCAGGTTTCCAGGGCGTCCTATTACCGGTGGCTAAACCCCTGCGAGGAGCTGAGCCAAGCCCAAGCACTGCGCAGGCGCCGCACCGAACTG
GTCCACACCGAATTCGAAGCATCCAACCAGGTTGCTGGGGCAGGTCAACTGGTGGACCTGCTCCGCAACAACCATGAGGAACAGATCCCGAAGTCTACGG
TCCTCTCGATCATGAAAGACCTGGGGATCCAAGCCAAACGGATGAGCGCGTTCAAGACCACGACGGTTCGTGATCATCAGGCCCGGACGGCGCATATCAA
GAACCATATGGTCGATAAGCTCGGCCGGCGGGACTTCCGCTCTTGCCGTCCTGGGGCGAAACTGGTTGGCGATATCACCTACCTGCGCACCGGCGAAGGC
TGGTTGTATTTGGCGACGGTCATCGATTTGTATTCGCGTCAGATCGTGGGCTGGTCAATGGGCGAGAACATGCAAACTCAGCTGGTCATTGACGCGATGG
AGCTTGCCGCGTCGCGGGGCTTTATCCAGCCTGGCACCGTGTTCCATTCAGACCGGGGCACGCAGTACACCTCTGGCGCGTTCCAGGCATGGTGCGCTGA
GCAAGGGGTGCGGCAGTCGATGGGGCGCACGGGGGTGTGCTGGGACAACTCCGTCGCAGAGTCAACCTTCTCGACGATCAAGAACGAAATGTATCACCAC
CGGGCTTTCACCAGCAGAGTAGCAGCGCGTACCGCGGTGATGGAATACATCGAGGTTTGGTACAACCGGAAGCGACCGCATACAGCTAACGGAGGGCTCA
GCCCTCAGGCAGTTCTGGACTCGTACCAGGAAAGAAATTCTTTAGCCTTGGCGGCTTGAGAATATTTAGAAAAGATCAAAGATCGTGTCTCAAGAACTTG
ACCAGCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 49 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MSKRQKFTPEFRAEAVELVISSQKSLVEVAASLGINEGTLGNWVRAYRTEHPEAETEERGPVEWAQYEKLRKELLEVKRENDFLKDVSAYFASNSKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1080 bp | 359 aa | 180 | 1259 | + | No |
Description : Second part of the transposase
ORF sequence :
PRLPHRAPRSGNRGTRTGGMGPIRKATQGTAGGETGERLFKRCQRLFRLEQQEISAIFTFIKAHAGQYPVNWMCQKLQVSRASYYRWLNPCEELSQAQAL
RRRRTELVHTEFEASNQVAGAGQLVDLLRNNHEEQIPKSTVLSIMKDLGIQAKRMSAFKTTTVRDHQARTAHIKNHMVDKLGRRDFRSCRPGAKLVGDIT
YLRTGEGWLYLATVIDLYSRQIVGWSMGENMQTQLVIDAMELAASRGFIQPGTVFHSDRGTQYTSGAFQAWCAEQGVRQSMGRTGVCWDNSVAESTFSTI
KNEMYHHRAFTSRVAARTAVMEYIEVWYNRKRPHTANGGLSPQAVLDSYQERNSLALAA
RRRRTELVHTEFEASNQVAGAGQLVDLLRNNHEEQIPKSTVLSIMKDLGIQAKRMSAFKTTTVRDHQARTAHIKNHMVDKLGRRDFRSCRPGAKLVGDIT
YLRTGEGWLYLATVIDLYSRQIVGWSMGENMQTQLVIDAMELAASRGFIQPGTVFHSDRGTQYTSGAFQAWCAEQGVRQSMGRTGVCWDNSVAESTFSTI
KNEMYHHRAFTSRVAARTAVMEYIEVWYNRKRPHTANGGLSPQAVLDSYQERNSLALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1211 bp | 403 aa | 49 | 1259 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKRQKFTPEFRAEAVELVISSQKSLVEVAASLGINEGTLGNWVRAYRTEHPEAETEERGPVEWAQYEKLRKELLEVKRENDFFKRCQRLFRLEQQEISA
IFTFIKAHAGQYPVNWMCQKLQVSRASYYRWLNPCEELSQAQALRRRRTELVHTEFEASNQVAGAGQLVDLLRNNHEEQIPKSTVLSIMKDLGIQAKRMS
AFKTTTVRDHQARTAHIKNHMVDKLGRRDFRSCRPGAKLVGDITYLRTGEGWLYLATVIDLYSRQIVGWSMGENMQTQLVIDAMELAASRGFIQPGTVFH
SDRGTQYTSGAFQAWCAEQGVRQSMGRTGVCWDNSVAESTFSTIKNEMYHHRAFTSRVAARTAVMEYIEVWYNRKRPHTANGGLSPQAVLDSYQERNSLA
LAA
IFTFIKAHAGQYPVNWMCQKLQVSRASYYRWLNPCEELSQAQALRRRRTELVHTEFEASNQVAGAGQLVDLLRNNHEEQIPKSTVLSIMKDLGIQAKRMS
AFKTTTVRDHQARTAHIKNHMVDKLGRRDFRSCRPGAKLVGDITYLRTGEGWLYLATVIDLYSRQIVGWSMGENMQTQLVIDAMELAASRGFIQPGTVFH
SDRGTQYTSGAFQAWCAEQGVRQSMGRTGVCWDNSVAESTFSTIKNEMYHHRAFTSRVAARTAVMEYIEVWYNRKRPHTANGGLSPQAVLDSYQERNSLA
LAA
Blast result :
Comments
ISAar25 is 58% aa similar to ISFsp8.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).