ISAar24
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 30/40
IRL : TGACATGACCTATGGCTCGGTTTCCAGTTGACGTCGCTAGTCGATGGTCT
IRR : TGACCTGCCCTACGGTTAGGTTTCCACTCTTGGTCGCTAGCTTCCGATGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAGGTGACA | GTT | GTGGTGTCAG | 3 |
DNA sequence
TGACATGACCTATGGCTCGGTTTCCAGTTGACGTCGCTAGTCGATGGTCTTCATGGAAGGCTCTTCGCATGCCACGCCCGTACCCTCCCGAGTTCCGTGC
CCGTGCCATCGCTCTGGTCCGCGAGGGCCGCCAGGTCAAGCAGACCGCACTGGACCTGGGCATCCACGAGGTCACCCTCCACAACTGGCTGCGACAGGAC
GATATCGACCTCGGCCGCCGGCCGGGACTAAGCAGTCAGGAATCTGCCGAGCTCCGCGCGGCGCGCGGCCGTGTCCGCCAGCTCGAGCAGGAGATCGCGA
TCCTCCGCCGGGCCGCGACGTGGCTCGACGAGGAGGGAGGAGTTGCCCCAAAAGGGCGCACCCGGTGATCGACCGACTCGTCGACGCCGGGGCACCCACA
AGCACCTGCTGCCGCCTACTGGGCGTCTCCCGTCAGGGCTACTACCGGTACCGAAAGCGCCCGACCAGTGCCACTGAGCTGCGCCGCCGCTGGCTGACCG
GCCTGATCCGGGAGATCCACGTCGCCTCCCGCGGCACCTACGGCTACCGCCGCATCCACACCGAGCTCACCATCGGAATGAACATTCCGTGTTCCAGCCG
GCTCATCTCCGTCCTCATGACTCGCGCCGGAATCGGTGGCCTTCCAGGCCCAGCCAGAATCAAGCGACTCAAGGGGGTCGCCACAGCGGACGACCTGGTC
AACCGCAAGTTCCACCGTCTCGCGCTGAACGAGCTGTGGGTCACGGACATCACCGAGCATCCCACCCGGGAGGGCAAGATCTACTGCGCCGCCGTGCTGG
ATGCTTGCAGCCGCAAGATCATCGGCTGGGCTATCGACTCCAAACAGGACTCCACCCTGGTCGTGAACGCTCTGGACATGGCAATCCGTGCACGTCAGCC
AGGGCCCGGAGGGATCGTTCATGCGGATCACGGAGTCCAGTTCACCTCCTGGGTCTTCACTCAGAAGATCCGTTCAGCGGGACTGCTGCCGTCGTTCGGG
ACCGTCGGCGATGGCCTCGACAATGCGATGATGGAGTCGTTCTGGTCGACGATGCAGATCGAGCTGCTGAACCGGAAGAAGTGGAAGACGCGCATCGAGC
TCGCGAACGCGATCTTCGAGTACATCGAGGTGTTCTACAACCGTCGTCGCCGGCACTCCTCCCTCGAGTACGCGACACCCCACGACTACGACCTCGCCCG
CACCCCGCGGGCACTCACCACCATCGGAAGCTAGCGACCAAGAGTGGAAACCTAACCGTAGGGCAGGTCA
CCGTGCCATCGCTCTGGTCCGCGAGGGCCGCCAGGTCAAGCAGACCGCACTGGACCTGGGCATCCACGAGGTCACCCTCCACAACTGGCTGCGACAGGAC
GATATCGACCTCGGCCGCCGGCCGGGACTAAGCAGTCAGGAATCTGCCGAGCTCCGCGCGGCGCGCGGCCGTGTCCGCCAGCTCGAGCAGGAGATCGCGA
TCCTCCGCCGGGCCGCGACGTGGCTCGACGAGGAGGGAGGAGTTGCCCCAAAAGGGCGCACCCGGTGATCGACCGACTCGTCGACGCCGGGGCACCCACA
AGCACCTGCTGCCGCCTACTGGGCGTCTCCCGTCAGGGCTACTACCGGTACCGAAAGCGCCCGACCAGTGCCACTGAGCTGCGCCGCCGCTGGCTGACCG
GCCTGATCCGGGAGATCCACGTCGCCTCCCGCGGCACCTACGGCTACCGCCGCATCCACACCGAGCTCACCATCGGAATGAACATTCCGTGTTCCAGCCG
GCTCATCTCCGTCCTCATGACTCGCGCCGGAATCGGTGGCCTTCCAGGCCCAGCCAGAATCAAGCGACTCAAGGGGGTCGCCACAGCGGACGACCTGGTC
AACCGCAAGTTCCACCGTCTCGCGCTGAACGAGCTGTGGGTCACGGACATCACCGAGCATCCCACCCGGGAGGGCAAGATCTACTGCGCCGCCGTGCTGG
ATGCTTGCAGCCGCAAGATCATCGGCTGGGCTATCGACTCCAAACAGGACTCCACCCTGGTCGTGAACGCTCTGGACATGGCAATCCGTGCACGTCAGCC
AGGGCCCGGAGGGATCGTTCATGCGGATCACGGAGTCCAGTTCACCTCCTGGGTCTTCACTCAGAAGATCCGTTCAGCGGGACTGCTGCCGTCGTTCGGG
ACCGTCGGCGATGGCCTCGACAATGCGATGATGGAGTCGTTCTGGTCGACGATGCAGATCGAGCTGCTGAACCGGAAGAAGTGGAAGACGCGCATCGAGC
TCGCGAACGCGATCTTCGAGTACATCGAGGTGTTCTACAACCGTCGTCGCCGGCACTCCTCCCTCGAGTACGCGACACCCCACGACTACGACCTCGCCCG
CACCCCGCGGGCACTCACCACCATCGGAAGCTAGCGACCAAGAGTGGAAACCTAACCGTAGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 69 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MPRPYPPEFRARAIALVREGRQVKQTALDLGIHEVTLHNWLRQDDIDLGRRPGLSSQESAELRAARGRVRQLEQEIAILRRAATWLDEEGGVAPKGRTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1053 bp | 350 aa | 182 | 1234 | + | No |
Description : Second part of the transposase
ORF sequence :
QLAATGRYRPRPPAGTKQSGICRAPRGARPCPPARAGDRDPPPGRDVARRGGRSCPKRAHPVIDRLVDAGAPTSTCCRLLGVSRQGYYRYRKRPTSATEL
RRRWLTGLIREIHVASRGTYGYRRIHTELTIGMNIPCSSRLISVLMTRAGIGGLPGPARIKRLKGVATADDLVNRKFHRLALNELWVTDITEHPTREGKI
YCAAVLDACSRKIIGWAIDSKQDSTLVVNALDMAIRARQPGPGGIVHADHGVQFTSWVFTQKIRSAGLLPSFGTVGDGLDNAMMESFWSTMQIELLNRKK
WKTRIELANAIFEYIEVFYNRRRRHSSLEYATPHDYDLARTPRALTTIGS
RRRWLTGLIREIHVASRGTYGYRRIHTELTIGMNIPCSSRLISVLMTRAGIGGLPGPARIKRLKGVATADDLVNRKFHRLALNELWVTDITEHPTREGKI
YCAAVLDACSRKIIGWAIDSKQDSTLVVNALDMAIRARQPGPGGIVHADHGVQFTSWVFTQKIRSAGLLPSFGTVGDGLDNAMMESFWSTMQIELLNRKK
WKTRIELANAIFEYIEVFYNRRRRHSSLEYATPHDYDLARTPRALTTIGS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 69 | 1234 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRPYPPEFRARAIALVREGRQVKQTALDLGIHEVTLHNWLRQDDIDLGRRPGLSSQESAELRAARGRVRQLEQEIAILRRAATWLDEEGGVAPKRAHPV
IDRLVDAGAPTSTCCRLLGVSRQGYYRYRKRPTSATELRRRWLTGLIREIHVASRGTYGYRRIHTELTIGMNIPCSSRLISVLMTRAGIGGLPGPARIKR
LKGVATADDLVNRKFHRLALNELWVTDITEHPTREGKIYCAAVLDACSRKIIGWAIDSKQDSTLVVNALDMAIRARQPGPGGIVHADHGVQFTSWVFTQK
IRSAGLLPSFGTVGDGLDNAMMESFWSTMQIELLNRKKWKTRIELANAIFEYIEVFYNRRRRHSSLEYATPHDYDLARTPRALTTIGS
IDRLVDAGAPTSTCCRLLGVSRQGYYRYRKRPTSATELRRRWLTGLIREIHVASRGTYGYRRIHTELTIGMNIPCSSRLISVLMTRAGIGGLPGPARIKR
LKGVATADDLVNRKFHRLALNELWVTDITEHPTREGKIYCAAVLDACSRKIIGWAIDSKQDSTLVVNALDMAIRARQPGPGGIVHADHGVQFTSWVFTQK
IRSAGLLPSFGTVGDGLDNAMMESFWSTMQIELLNRKKWKTRIELANAIFEYIEVFYNRRRRHSSLEYATPHDYDLARTPRALTTIGS
Blast result :
Comments
ISAar24 is 55% aa similar to ISMsm7.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).