ISfinder Mirror Site

ISCysp13

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAXW01000052	ND	Cyanothece sp.	Cyanothece sp. CCY 0110

DNA section

IS Length : 1836 bp

Ends

Left end : CTTCAGTGCGTCAGTCCCTAGTCTCAGCGTAGCGGACTAGGGAAGGAAAGCACGACAATTTGAGGACTCAATGTCCGAAAAATTGTCAAATTAACTTTTC II struct. : Yes
Right end : CTTTATGCGGAAGCATGAGGCAACTAGGGGCGAAAAAGCACAACAATGCAAAGAAGCGTCAGAAACATCTAGCTCAACAAAGTGGCTAGATGTAGTTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ACTATTGCTAAAGAAGGCGG	TGAA	TTTCTAATCACCTTTCCGGA	TCAT
GGCATCACCAAAATTTTAAT	TGAA	AGCAAAGACGAAGCCGTTGA	TCAT
AATTTAACGACTTGAGTACC	TGAA	TAATTTGCGCTTGAGAACGA	TCAT

DNA sequence

CTTCAGTGCGTCAGTCCCTAGTCTCAGCGTAGCGGACTAGGGAAGGAAAGCACGACAATTTGAGGACTCAATGTCCGAAAAATTGTCAAATTAACTTTTC
GGAGCATCTTGGTTCTTGATATATTCTTTTAACTTTTCAATAGGTGCGCCACCAGAAGAAGTGACATAATAAGACTTAGACCAAAAAGAAACTTTGTCTT
TATAAAACTTAGTTACTTGTTCAGGAAAGTCTTTTTTCATGACTCTAGCTGTCGAACTTTTAAGACACCCTGCAACTGTAGAAATAGAGTTTTTAGGATG
GAAATCTAATAAGATATGGACATGATCCATTTCACCCGAAAACTCTAGTAACTCACAATCCATTTTGCGAGATACTTGCCAAATCATTTCTCTTAATCGC
TCTAGCATTAGGGCGGTTATTACTTTGCGTCTATAGTGAGTAACAAAAACAAAGTGTAACCGCACACTAAAAACACTATGTGACCCTTTTCTTGGTTTCA
TCTTGACACCCTACAGGTAATTAGACTAAAATCATCATATCAGTTCTGTTGAGGTCGGCAACATGGCTACTAAAAGAATCACGTTTAGACTTTACCCAAA
TAAAGAACAAAATGAGAAACTTCATTATTGGAGACGTTTACACAAAGATTTATACAATGCTTGTGTAGTTAATCGAAAAACTCAATACAAAAAGTTTGGT
AAATCCATTAATTACTTTGACCAACAAAACTCATTACCTGAGTTTAAAAAAGATTGGCCAGAATACAAAGACCTTGGTAGCCATGCCCTTCAAGCAACTG
TAAAACGGGTTGATTTTGCTTTCCAACGTTTTTTCAAGATTAAATCAGGTTATCCGAAACTAAAGTCTTCAAGAAGATATAAAGGGTGGACTTATCCTGA
TAAAGCTGGTTGGAAAATAGACAGTAATGGACATCATGGAAAGTTGACACTTTCTAATTTTGGAACAATAAAGATGAGGGGTAAAGCAAGAGATTGGGGA
ACACCAAATACTTGCACTATCATCTTTAAACAAGGAAAATGGTACGCTTCTGTTACTGTTAGTTGTGATCCAAAACGAGAAACAGGTAAAGAGGCTTGTG
GTATCGATTTTGGGACTCATCATGCACTTGCTTTTGACGATGGCACTGTTATTGATAATCCTAGATTTCTCAAACAATCACAGGATAAAATCAACAAAAC
AGCAAAGAAATCTAGAAAGAAACGTGCAGGAAAAAAGGGAGTAAAAGGGTCTAGAAGATGGAAAAAAGCTGTTAGAAAAATCGGTAAGATTCAATCTTTA
GTTGGTCGTCAAAGACAAAACTGGCATCATCAAGTTTCCACACAAATAGTTAGCTGTAATAGCTTGGTGGCAACTGAGAAATTAAACCTAAAAGGAATGA
CCAAAAAAGCTAAAAGAGGTAGCAAAAGAAAACGTCAAAAGACTGGATTAAATCGGTCAATTCTTGACGTTGGAATTGGTAACTTAAAATCATTAATAAA
AAGCAAAGTTACCGAAGCAGGTGGGTTTTACATTGAGGTTCCTACTCGAAAAGTTAAGCCATCTCAGACTTGTCCTAATTGTGGTCATCAAAAGAAAAAG
ACATTAGCAGAACGCCAGCACAACTGCGAAAAGTGCCTTTATAGTTGCGACAGAGATGTTGCATCAGCCCAGGTGTGTTTAAACTGGGCAAGGGGGCAGG
AACTGTCCTCTTTAGACGTAGAGTCGCCTAGCTCTACTTTATGCGGAAGCATGAGGCAACTAGGGGCGAAAAAGCACAACAATGCAAAGAAGCGTCAGAA
ACATCTAGCTCAACAAAGTGGCTAGATGTAGTTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
318 bp	105 aa	408	91	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MLERLREMIWQVSRKMDCELLEFSGEMDHVHILLDFHPKNSISTVAGCLKSSTARVMKKDFPEQVTKFYKDKVSFWSKSYYVTSSGGAPIEKLKEYIKNQ
DAPKS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1263 bp	420 aa	563	1825	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MATKRITFRLYPNKEQNEKLHYWRRLHKDLYNACVVNRKTQYKKFGKSINYFDQQNSLPEFKKDWPEYKDLGSHALQATVKRVDFAFQRFFKIKSGYPKL
KSSRRYKGWTYPDKAGWKIDSNGHHGKLTLSNFGTIKMRGKARDWGTPNTCTIIFKQGKWYASVTVSCDPKRETGKEACGIDFGTHHALAFDDGTVIDNP
RFLKQSQDKINKTAKKSRKKRAGKKGVKGSRRWKKAVRKIGKIQSLVGRQRQNWHHQVSTQIVSCNSLVATEKLNLKGMTKKAKRGSKRKRQKTGLNRSI
LDVGIGNLKSLIKSKVTEAGGFYIEVPTRKVKPSQTCPNCGHQKKKTLAERQHNCEKCLYSCDRDVASAQVCLNWARGQELSSLDVESPSSTLCGSMRQL
GAKKHNNAKKRQKHLAQQSG

Blast result :

Comments

ISCysp13 is 66% (ORFA) and 51% (ORFB) aa similar to ISCARN6.

References

1] ISfinder annotation (2009)
2] Stal,L., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.