ISfinder Mirror Site

ISAtu6

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010929	ND	Agrobacterium tumefaciens	Agrobacterium tumefaciens Ti plasmid pTiBo542 Agrobacterium tumefaciens

DNA section

IS Length : 2798 bp

Ends

IR Length : 23/24

IRL : GTGAGCGTCCGGTGAGCTGTTTTTGCTGATCGGGCAAATCAGGCGATGTT
IRR : GTGAGCGTCCGGCGAAAGCGTTTTCGGGTATCGAGAGATGGAAGCTATGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGTAAATACC	GCCAATGA	GGTGCGCGAT	8
CGAAAAGATA	GTCTTTAT	CACCGCGCTT	8
GTAGAGCGTT	GTTCAGCG	AAATACTAGC	8

DNA sequence

GTGAGCGTCCGGTGAGCTGTTTTTGCTGATCGGGCAAATCAGGCGATGTTCTGGCATTCGAACCAGCATTAGTGCTGACATTAATACCAGAACAAAGAGG
TTTCATGGCTCGCATGGAGATCATGTCCGGTACCGAGCGCAGGCGGCGCTGGTCGGACGAGGCGAAGCTCAGGATATTGGCGGAAGCTGATGAACCCGGT
GCTCGCATTGGTGAGGTGGCGCGCCGGCATGACGTTCATCCTGGCCAGATCCGCTTGTGGCGGAGGTCGTTCAACTATGTGGATCGACCCACGGTGTTCC
TTCCAGTGGAAATCATGGAGGAGGCTGGCGTAAGTCAGGCGTCTTCGACGGTAACGAGGCCGGCGATCGTCGAGATATTGCTGCGGAACGGTCGGTGCCT
GAAGGTTCCTGCTGACGTTGAGTTGAAGCTGCTCGGTCCGCTGATCGCTTGCGTGGAGGCGGCATGATCGGGCCATCGGGCAATGTAAGGGTCTATCTGG
CCTGCGGAGTGACCGATATGCGGCGTGGCATTGATGGCCTATCGGCGCTGGTCGAGACGGTCGTGAAGGAGGCGCCGGGCTCGGGCGCGATTTTCGGCTT
CCGCGGAAAGCGCGCCGACCGGATCAAGCTTCTCTGGTGGGACGGCCAGGGGTTCTGCCTGTTCTACAAGATTTTGGAGCGTGGATACTTTCCCTGGCCG
ACAGCGAAAGAAGGTGTCGCGCACCTGACGCAGGCGCAGCTTTCCATGCTGGTTGAGGGGATTGATTGGCGCCGCCCGGCATGGACGTCCGCTCCTGGCC
GAACAGGATAAAAGCTATATTTTGCAAGGGCATCCGAGGCTTAACGCTGGCGCGTCAGAGGCAAATCGGCTAGATTTGCGGATATGGAAACAGCGCCGCT
GGACAGTCAGGACGAACTCACTGCTTTGCGCGCACTGGTCGCCGAACAGGCGGCGAAGCTTGAGAGCCAGGAAGCCGAGGTCATCAAGCGAGACTCCATC
ATAGGGCTTCTTCGCGCGCAACTGGAGCTTCTCCGACATCGGCAGCATGGCGCCTCTTCGGAAAAGATCGACCGGAAGATAGAGCAGTTCGAACTGATGC
TGGAGGAGATTGAGGCTTCCCGTGCCGAGGCTGAACTGCGCTCCGGGAAAACACCCTTGCCGGATTTGGACGACGCGCCGGACAAGCCGAAGCGCAAACC
ATTGCCCGATGGTCTCGCCACCGAAGAGCTGATCTATGCGGCTCCCTGTAATTGCCCGACCTGCGGTGGCACCTCGTTCCTGAAGGCGCCCGACAGGGTG
GTTCAGGTGCTGGAACACGTGCCGGCGTCGGTCAAGATTGTCCGCCATGTCGAGAAGCGTATGATCTGCAAGGAATGCGATACGACAGTGGCTGGCGAGA
TGCCGACCTTGCCGATCGAGCGCGGCAAGCCCGGGCCTGGATTGCTCGCCCATATCATGGTCTCCAAGTTCGATGATCACATTCCGCTTTACCGTCTCTC
AGAGATGTATGATCGGCTGGGAATAGACATATCGCGCTCCGTGATGGCCGACTGGGTCGGCCGCGTATCCGCTTTGCTGACACCCATCGTCTTGTTGATC
AGGGCCCACATCGCCGCGCTTGACCGAATACATACGGACGATACCCCGGTCGATGTTCTCGACCCCGGACGGGGCAAGACAAAAACCGGCAGGGTCTGGG
TCTACGTCTTCGACGGCAGTGGCTATCAAGCCACCACTCCGGCAGCCATCGCCTATTACTACAGTCCTGATCGAAAGGGCACACATCCGGCTGACCACCT
GGCAAGCTTTAGCGGCGTCATGCATGCCGACGGTTATGGCGGCTACAGACAACTCTACGGCAACCAGATCGTTGAGGCCGCCTGCATGGCGCATGTACGT
CGCAAGTTCCATGACGTGATCAAGCTGAAGCCATCGCCGATCGCCGACGAAGCGCTGTCGCGCATCGGCGCGCTCTACGATATCGAGGATCGTATCCGCG
GCATGTCGGCTGATGAGCGTCGTACCCTGCGCCAACACCACGCCAGACCCATTCTGGACGAGCTGAAGACCTGGATCGAGGCGACACTCTCAACTTTGCC
ACAGAAGCAGAAGCTGGCCGAGGCAATGCGATATGCGCTGTCTCGATGGGCAGCCTTGAGCGTTTACATCGACGATGGCCGTGTCGAAATCGATAACAAC
ATAGCTGAGCGAGCGATGCGTCCGCTGGGCCTCGGCAGAAAAAACTGGTTATTCGCAGGCTCGGACAAGGGCGGTGAGCGCATCGCCAACATCCTGACCA
TCATCGAAACGGTCAAACTGCACGGCCATAATCCGGAGCTCTACCTGACAGATGTCCTGACCCGGATCCAGGATCACCCCAAAGACCGATTTGAAGATCT
GCTGCCCTGGAACTGGATGCCAGCAAAAGCTCGATGCGAGGCCGCCTGATGGCTCGCTCGAGGTTCATCTATACGCTCAGCCAAGTCGCCGGCATGATCG
GCGAAAACCTCGAACTGATCGAAGAAGTAACCGCAAACCCGGACAACATCTCCGAGGGCGAACTGGTTTACGTCAGCGATGGCAGTGAAGATGGCACGAA
GGGTCTGACCGGGAATGGCATCGAAGAACTTCAAAGCCTACTTGCCGACATCAGGACGTGGGACGGCGGTATCCGCGAGTTCCTCATCGACACGCAGTGC
GATCCTGAAATGATCGACCGCGTCATGGCCGATGAAATGAAACGCGGCTCATAGCTTCCATCTCTCGATACCCGAAAACGCTTTCGCCGGACGCTCAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	114	467	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MEIMSGTERRRRWSDEAKLRILAEADEPGARIGEVARRHDVHPGQIRLWRRSFNYVDRPTVFLPVEIMEEAGVSQASSTVTRPAIVEILLRNGRCLKVPA
DVELKLLGPLIACVEAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	464	811	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIGPSGNVRVYLACGVTDMRRGIDGLSALVETVVKEAPGSGAIFGFRGKRADRIKLLWWDGQGFCLFYKILERGYFPWPTAKEGVAHLTQAQLSMLVEGI
DWRRPAWTSAPGRTG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1566 bp	521 aa	884	2449	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

METAPLDSQDELTALRALVAEQAAKLESQEAEVIKRDSIIGLLRAQLELLRHRQHGASSEKIDRKIEQFELMLEEIEASRAEAELRSGKTPLPDLDDAPD
KPKRKPLPDGLATEELIYAAPCNCPTCGGTSFLKAPDRVVQVLEHVPASVKIVRHVEKRMICKECDTTVAGEMPTLPIERGKPGPGLLAHIMVSKFDDHI
PLYRLSEMYDRLGIDISRSVMADWVGRVSALLTPIVLLIRAHIAALDRIHTDDTPVDVLDPGRGKTKTGRVWVYVFDGSGYQATTPAAIAYYYSPDRKGT
HPADHLASFSGVMHADGYGGYRQLYGNQIVEAACMAHVRRKFHDVIKLKPSPIADEALSRIGALYDIEDRIRGMSADERRTLRQHHARPILDELKTWIEA
TLSTLPQKQKLAEAMRYALSRWAALSVYIDDGRVEIDNNIAERAMRPLGLGRKNWLFAGSDKGGERIANILTIIETVKLHGHNPELYLTDVLTRIQDHPK
DRFEDLLPWNWMPAKARCEAA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
306 bp	101 aa	2449	2754	+	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MARSRFIYTLSQVAGMIGENLELIEEVTANPDNISEGELVYVSDGSEDGTKGLTGNGIEELQSLLADIRTWDGGIREFLIDTQCDPEMIDRVMADEMKRG
S

Blast result :

Comments

ISAtu6 orfA is 50% aa similar to ISPpa5_aa1.
ISAtu6 orfB is 77% aa similar to ISPpa5_aa2.
ISAtu6 orfC is 66% aa similar to ISCARN48_aa3.
ISAtu6 orfD is 55% aa similar to ISPpa5_aa4.

ISAtu6 was reconstructed by deletion of ISAtu7 sequence (family IS110).

References

1] Oger,P.M., Farrand,S.K., Olsen,G.J. and Reich,C.(2005) Direct submission GenBank.
2] ISfinder annotation (2009)