ISAzba8

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum brasilense
Azospirillum brasilense plasmid p2AZOBRv1
DNA section
IS Length : 1321 bp

Ends


IR Length : 31

IRL : GGGAGTGTCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCGAAAGGA
IRR : GGGAGTGTCCGGAATTTCGTGCTCGGCGGGGCGGGTTGAGTATCAGGCGT

Insertion site


Left flankDirect repeatRight flankDR Length
GGGATCAGCAGCCACTACACGAGGCGTGTC9

DNA sequence

GGGAGTGTCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCGAAAGGAGCCCCTGCATGGCACGACGCAAAGAGCCGGTCATCCCGGACGCAATCCTC
GACCAGCTGTTGGCCGGTGCTGACGCCAAGACGGCGTTCGATCCGAACGGCCTGCTCGACCAATTGAAGAAGGCGCTGACGGAGCGGGCGCTGAAGGCGG
AGTTGGATCATCACCTGGCCGGTGACGAGAGTGGCAACCGGCGCAACGGCTACGGCCGCAAGACGGTGCTGACCGAGCGTGGGTCGATGGACCTGTCCAT
TCCACGCGACCGGACGGGCACGTTCGACCCGGCGCTGATCGCCAAGTACCAACGGCGCTTCCCCGGCTTCGACGAGAAGATCATCTCGATGTACGCGCGA
GGCATGTCGACGCGGGAGATCACCGGGCATCTGCGGGAACTCTATGGCATCGAGGTCTCGGCCGACCTGATCTCCACGGTGACGGACGCGGTGATCGAGG
AGGTGACGACGTGGCAAAACCGGCCCCTGGAGGCGATCTACCCGCTGGTGTTCCTGGAAGCCCTCCGGTCAAGATCCGGGGAGAAAGGCTGGTGGGCAAA
CAAGGCCATCCAGGTGGCCATCGGCGTGCGCGCCGACGCGCCCAAGAAGGTGCTGGGCCTGTGGATCGAGCAGACCGAAGGCGCCAAGTTCTGGCTGCGC
GTGCTGAACGAGCTGAGGAACCGGGGCGTCGAGGACATCCTGCTGGCCGTGGTCGACGGGCTGAAGGGCTTTCCCGAGGCGATCGCCGCCGCCTATCCCG
AAACCATCGTCCAGACCTGCATCGTCCACCTGTTGCGCCACAGCATGGAGTTCGCGTCCTGGAAGGAGCGCAAGACCATCGCCGCCGCGCTGAAGACGGT
CTACGACGCCGTCGACGACAAGGCCGCCGAGGCGGCCCTGACCGCCTTCGAGGACGGACCCTGGGGTGAAAAGTACGCGGCCATCGGCAAGGCGTGGCGA
CGGGCGTGGCAGGAGGTCATTCCCTTCTTCGCCTTCCCCCGCGAGGTCCGGCGCATCCTCTACACCACCAATGCCATCGAGGCTTTGAACTCGAAGCTGC
GCCGGGCGGTGCGCGCCAGGGGCCACTTCCCCAATGACGAGGCGGCCCTGAAGCTCCTGTTTCTCGTCTTGAACCGCGCCGAGAAAGACTGGAAGATGCC
GCCGCGCGAATGGACGGCCGCCAAGGCGCAGATGGCCGTCATCTTCGGCGAGCGGTTCGCAAAGGCGATGAACGCCTGATACTCAACCCGCCCCGCCGAG
CACGAAATTCCGGACACTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1221 bp406 aa591279+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARRKEPVIPDAILDQLLAGADAKTAFDPNGLLDQLKKALTERALKAELDHHLAGDESGNRRNGYGRKTVLTERGSMDLSIPRDRTGTFDPALIAKYQRR
FPGFDEKIISMYARGMSTREITGHLRELYGIEVSADLISTVTDAVIEEVTTWQNRPLEAIYPLVFLEALRSRSGEKGWWANKAIQVAIGVRADAPKKVLG
LWIEQTEGAKFWLRVLNELRNRGVEDILLAVVDGLKGFPEAIAAAYPETIVQTCIVHLLRHSMEFASWKERKTIAAALKTVYDAVDDKAAEAALTAFEDG
PWGEKYAAIGKAWRRAWQEVIPFFAFPREVRRILYTTNAIEALNSKLRRAVRARGHFPNDEAALKLLFLVLNRAEKDWKMPPREWTAAKAQMAVIFGERF
AKAMNA

 

Blast result :
Comments
ISAzba8 is 78% aa similar to ISRm3.
References
1] ISfinder annotation (2009).