ISAar22

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1722 bp

Ends


IR Length : 37/50

IRL : TGTCCTCTCTCAGAACATCGCTTACACCCTGACTCACAACATCGCTTACA
IRR : TGTAATCACTCGGTAGTTTGTTTACACACGAACTCACCACATCGCTTACA

Insertion site


Left flankDirect repeatRight flankDR Length
GTATAGGTGTATGGAAACTCGAGCTGTGCTATTCG15
GTACTACTAATCCGGCGAGTCGGGCCGGCGAGTCA15
ATACCGGTGCCAGCGCAATGGCAATGCCCGAAATG15
GTACCCGTTATTGCTCGGAACGCTGGATCCGGTCG15
GTACCGGCAGTCCGGCAGTCCGGCTAAGTGGTACT15
ATACCGACTGCGAGATGGGCTGTCACTGGCAGCCG15
GTTCCGCCGATGGTAGg/aATGTGACATCGTATACTA17

DNA sequence

TGTCCTCTCTCAGAACATCGCTTACACCCTGACTCACAACATCGCTTACAGGATGTATCAGCACATCGCTGACAGGTAGCAAGACATGCTGGCAGGCCCC
TTCTGGTCAGCGATCAATTTGATAATTAAACGTGTCAAATTCATTATCGGCGTCGATACGCCGCCAAATCATCGAGTTCGATCCGGGCCTGCCAGACTCC
TTGAGTATCTCGCAGTTCTGCAAGGAACTGGGAATCAGCAGACCCTCCTACTACAAAGTCAAAGAGCGATTCGTTGCCGAAGGGAACAAAGCACTCAACC
CGCACTCCAGAGCCCCGAAAACAGACCGGCGCATCTACAGCGACCAAACCAAAACCACGGTCCTACGCATCCGCAAGCGACTGGCCAAGGACGGGTGGGA
CAACGGGCCCTTATCTATCTGGTTCGAAAGCCTTGATACTCAGGAGTTCGGTGAGCTACGCCCCTCGGTGGCCACGATCGGCAGGATCCTTGCCGAGGCC
GGAGCTACCAAGCGAAACCCGCGCAAACGCCCCCGTAAATCATGGCTGCGCTTCGCCCGTTCGCATCCGATGGAAATGTGGCAGATCGACGGACTGGAAT
ATCGATTGTTCGACCAGGACGCGACCAAGGCGATGATCTATCAGCTCATTGATGACGGCTCCCGATTCGACGTGGGTACGCGGTGCTTTGAGCAAATGGA
GAACGCGCAAGATGCGATGGAAACGCTCAAGGCAGCGTTCGCGGAGTATGGGGTGCCGCAACAACTTCTCAGCGACAATGGTGGTGCTTTCAATCTTTCT
CGGCAGGGCCTGGTGAACCAGACTGAAATATTCTTGGCTAGCGTGGGCTGCGAAGCGATTACGGGCCGGTTCAGTCATCCGCAAACGCAAGGCAAGAACG
AACGCAGCCATAATACGTTGACCCGGTTCCTCGACGCGCATGCACCACATAACCTGGCGGAGCTTTCCACGTTGTTGGAGCAGTATCGGAATCACTATAA
CCACCGTCGGCGCCACCAGGCGCTGAAAGTGGGGCACACGTATCTGACGCCTGCTCAAGCGTGGGAAGCCGGTGATCATCGTGGTTCGGATGGCGTGGCC
ATCGATATTGCCAGGATACAGGCCAAAGCCCAGTCGTACTTGGACAAGGCTCTGGCCGTGAAAGCCGATTTGGTTCCGGAGGGCGTAGTGGATGATGGGC
TTCAGGTGTTGGGACGTACGAAGGTTGAAAACTACACCGGTAGCCCCAGCGTACTGACGGATCAACGCGATGATGTGATCCAGATTCGACGGACGAATCC
GCAGATCTATTTTCGTGGCCGTGTTTTCAAGGTGCCAGCTCACCTGATCGGTGAATACCAGCTGGTCTTGAGCAAGGATGCTTATACGCTTTACAGCACT
GTTGATGGTGAGCAATCACTGTCATTTCCGTTACCGGTCAGGCTGCATTCCAGTGCGCGTTTGGTGCCGTTGTGGCAGGTGTATGGAGCCCGGATCAGGG
ATCCGAAGCCGGCCTGGACTCAGAAGCGGATCGAGTATGAGGACATCTACTATTCGTCTGACGTCGCCGTTTCCTAGCTAAGGAGATTCGTTGCGCACGG
TGTCAGCGATGTTGTGAGTTTGGTTGTCAGCGATGTGCTGATGCCGCGTGTCTACGATGTCCTGAGTTTGGTTGTAAGCGATGTGGTGAGTTCGTGTGTA
AACAAACTACCGAGTGATTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1446 bp481 aa1321577+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VSNSLSASIRRQIIEFDPGLPDSLSISQFCKELGISRPSYYKVKERFVAEGNKALNPHSRAPKTDRRIYSDQTKTTVLRIRKRLAKDGWDNGPLSIWFES
LDTQEFGELRPSVATIGRILAEAGATKRNPRKRPRKSWLRFARSHPMEMWQIDGLEYRLFDQDATKAMIYQLIDDGSRFDVGTRCFEQMENAQDAMETLK
AAFAEYGVPQQLLSDNGGAFNLSRQGLVNQTEIFLASVGCEAITGRFSHPQTQGKNERSHNTLTRFLDAHAPHNLAELSTLLEQYRNHYNHRRRHQALKV
GHTYLTPAQAWEAGDHRGSDGVAIDIARIQAKAQSYLDKALAVKADLVPEGVVDDGLQVLGRTKVENYTGSPSVLTDQRDDVIQIRRTNPQIYFRGRVFK
VPAHLIGEYQLVLSKDAYTLYSTVDGEQSLSFPLPVRLHSSARLVPLWQVYGARIRDPKPAWTQKRIEYEDIYYSSDVAVS

 

Blast result :
Comments
ISAar22 is 54% aa similar to ISArsp2.
References
1] ISfinder annotation (2009)