ISfinder Mirror Site

ISStsp3

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NW_002062883	ND	Streptomyces sp.	Streptomyces sp. SPB74

DNA section

IS Length : 1925 bp

Ends

IR Length : 11/13

IRL : CCTCTGCGGCTGTGCGCTCGGTCCAGGTGCCCGTTAGCCTCGCGCTTTCA
IRR : CCCTGGGGCTGTCGGTCCAGGCGTTGAGTGGAGGCCAGCCCGATGCTGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTGTAGTAAG		CTTGGGCTAT	0

DNA sequence

CCTCTGCGGCTGTGCGCTCGGTCCAGGTGCCCGTTAGCCTCGCGCTTTCATGAAGCGGGCTGTCCGGCAGGCGGTCAGGAAAGCAGAAAGTGCCTCTGAC
CAGCAAGAATGAGGATTGTCGAGGTCCTTGTTCCTGCCACCGCCGGAGGCACTTCCCAGATGAAGCACTCTATCGGGTCCTATCCCCGTGTCCGCGTCCA
GGACGACGGTCGCCAGGTCGTCTCCCAGGCCGGGACGGTCCTGTTCGTCGAAACGGCCCGCAAAACCGGTCTTGACCAGGTCATATCGGCAGCTCTGGCG
CCGTGGCGCAAGCCGCGGGCCGTCCACGATCCTGGCAAGGTCCTCCTGGATGTCGCCCTGGCGGTCGCACTGGGCGGGGACTGCCTCGCGGATGTCGCCG
TGCTGCGGGCCGAGCCGGCCGCCTTCGGTCCGGTCGCCTCCGACCCGACCGTCTCCCGCCTGATCGACGCCCTCGCCGCCTCCGGCGAGAAAGCTTTGCA
GGCCATCCGGTCCGCACGCTCCGAAGTCCGTGACCGTGTCTGGTCGTTGGCCGGTGAGAACGCCCCGGACACCGGCGGGCAGGTCGTCGTCGATCTTGAC
GGCGTCCTCGTGATCGCGCACTCCGACAAGGAGGACGCGGCCGCGACCTGGAAGAAGACCTACGGTCACCATCCGCTGACGGCCTTCGTCGACCACGGCC
CGGGCGGAACCGGCGAGCCGGTCGCCGCCCTCCTCAGGCCGGGCAACGCGGGCTCGAACACAGCGGCCGACCACATCACCACCGCCCAGCTCGCCCTGGC
CCAGCTGCCCAAGCGTTACCGGCGAGGGCGGCAGACGCTGATCCGCACGGACTCCGCCGGCGGCACCCACGACTTCGTGTCCTGGCTCGCCCGGCGAGGA
CGATGGCTGTCCTACCCGGTCGGAATGACGGTCACCGAGGCGATCCACGAGCATGTGCTGAAGGCCCCCGCATCGGCCTGGACGGCGGCCGTCGAGACCA
ATGGCGAGGTCCGTGACGGGGCCTGGGTCGCCGAGCTCACCGGCAAGCTGCTGGACGGCTGGCCCAAGGGCATGCGACTGATCGTCCGAAAGGAACGGCC
CCACCCCGGCGCCCAGTTGAGGATCACGGACGCGGACGGCATGCGGATCACGTGCTTCGCGACCAACACCACCGGTCGGCCGATCGCCGAACTCGAGCTC
CGTCACCGGCTACGGGCCCGGGCGGAGGACAGGATCCGGGCCGCCCGGGCCACCGGCCTGCGCAACCTGCCCCTCCACACCACAGCCCAGAACAAGGTCT
GGCTCCGGATCGTCCAGATCGCCCTCGACCTGCTGGCCTGGATGCCCATGCTCGCGCTCACCGGCAAGGCCAGGCTCTGGGAGCCCCGCCGCCTGCGGTT
CCGCCTGTTCACCGCGGCCGGGCAGCTCGTGACCACCGGCCGTCGGCGGATCCTCCGCCTGGCCCGGCACCGGCCCTGGACCGGCCACATCACCGCCGCA
CTCGAACGGCTCGCACTCCTGCCGAATCCCGGCTGACCAGCGGATTCACCCATCCCTACGACAGCTCTTCACCACCCGGAGCAGTGGAACCCGGCGCCAC
CCCGAGACGCACTCGGGCCCTCGGCCTGCCCAGCCTCAGCTCACGGCACGAAAACAGGCCACCGACTGCGTCGGCGGCCCGTCACGAAACTTCGAGGTTA
GTGCCGGGCCATCCTTGGTGAAGTCCAGGAAGATCGGGGAGTCGTTGGACCCGGGCAGGCTTTCGCCGTGCAGTCGGTTGCCCAACTGCCGTACCACTAC
GTAGTGGTGCCGGGTCACCTGCCCCCGCTTCGTGCTCTCGTAGGTGTAACGGCTGTGCCAGATTCCCGAGAGGCTGTCAGCATCGGGCTGGCCTCCACTC
AACGCCTGGACCGACAGCCCCAGGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1377 bp	458 aa	160	1536	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKHSIGSYPRVRVQDDGRQVVSQAGTVLFVETARKTGLDQVISAALAPWRKPRAVHDPGKVLLDVALAVALGGDCLADVAVLRAEPAAFGPVASDPTVSR
LIDALAASGEKALQAIRSARSEVRDRVWSLAGENAPDTGGQVVVDLDGVLVIAHSDKEDAAATWKKTYGHHPLTAFVDHGPGGTGEPVAALLRPGNAGSN
TAADHITTAQLALAQLPKRYRRGRQTLIRTDSAGGTHDFVSWLARRGRWLSYPVGMTVTEAIHEHVLKAPASAWTAAVETNGEVRDGAWVAELTGKLLDG
WPKGMRLIVRKERPHPGAQLRITDADGMRITCFATNTTGRPIAELELRHRLRARAEDRIRAARATGLRNLPLHTTAQNKVWLRIVQIALDLLAWMPMLAL
TGKARLWEPRRLRFRLFTAAGQLVTTGRRRILRLARHRPWTGHITAALERLALLPNPG

Blast result :

Comments

ISStsp3 is 70% aa similar to IS1676.

References

1] Isfinder annotation (2009)
2] Fischbach,M., Ward,D., Young,S., Jaffe,D., Gnerre,S., Berlin,A., Heiman,D., Hepburn,T., Sykes,S., Chen,Z., Alvarado,L., Kodira,C.D., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Lander,E., Galagan,J., Nusbaum,C. and Birren,B. (2008) Direct submission GenBank.