ISAcr5

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009484 ND Acidiphilium cryptum
Acidiphilium cryptum JF-5
DNA section
IS Length : 1618 bp

Ends


IR Length : 12/17

IRL : CCTGAATTATGCGTGAGGGTTGGCAGGCATAGCGTAAACCGTTGATTTGA
IRR : CGGGATTATTCGCGAGCGCATTTCCTCTGATTTTTCTCAGGCGGGTTGTG

Insertion site


Left flankDirect repeatRight flankDR Length
CGGTTCTTAGCTAAGTTGAG0

DNA sequence

CCTGAATTATGCGTGAGGGTTGGCAGGCATAGCGTAAACCGTTGATTTGATCTAAGAATTTGGTGTCGAGACCAAATCTTCATCAGCATGGAACGTGATG
CCCGCCACGGTTGAAACGACCCCATGCCTCCCCGGTCTGTCACCTGTCGCCGGCAAACCGGTGCTGGCCCGCTTCGACGGCGAGATGCTGTCCTCAGACG
GCGGTCTGCTGGCATTACGCGAGGTCGAGAAGCGGCTGGGGATTGCCGCGCAGTTGGCAGCCTGCATCGATGATCCGCGCACGCCGGAGGGCGTCCAGCA
TGGGCTCGACGAGATCATTCGGTTTCGCATGTTGATGATCGCGGCCGGTTACGAGGATGGCAACGACGCCGACCGGTTGCGCAACGACCCGATGTTCAAA
CTCGCGATGGAGCGTCTGCCCGAGGCGGGCGATCTGTGCTCGCAAGCGACGGTTTCACGCACCGAGAATTTGCCCGGGCCTCGCGCGCTGCTGCGCATGG
GCCTGGCGATGGTCGAGCATTATTGCGCGAGCTTCCGCACCATACCCAACCGGATTGTGCTGGATATCGACGATACCTTCGATGCCGCTCACGGCGCGCA
GCAACTCTGCTTGTTCAACGCACACCACAACGAATACGGATCTCAACCGATTGTGGTGTTCGACGGCGACGGCCGGATGCTCGCCGCGGTGCTGCGTCCG
GCGTGTCGGCCAAAAGGGGCCCAGATCGTCAAATGGTTGCGCCGGCTGATCGACGCCATCCGCGGCCATTGGCCACGTACCGCGATCATGCTGCGCGGTG
ATTCGCACTTCTGCACGCCGGCAGTTCTGCGGTTCTGTCGAGCGCGTCGCCTCGATTACATCTTCGGCGTCGCACGACCACGACGCTGCGCAAACACGTC
ATCGCGCTGGAGGCCAGCACCACGGCGCGCGCGCAACAGGCTCCGGGAGAGAAAATCCGGCGGTTCAAAGAGTTCAATGATGGCGCAGCAAGCTGGGACC
GCGTCGAGCGGATCATCGCCCGCGTCGAGGCCTGGCCGCTGGGGGGCGATACCCGCTTCATCGTCACCAGCCTGAAGGCTGGTTCCCCGCGCACCCTGTA
TCAAGAGATCTACTGCGCGCGCGGCCAGGCCGAGAACCATATCAAAGGCGTGGAAGACCCATCTTGCGGCCGACCGGACCTCGTGCTCGCGGGCCAGCGC
CAACCAGATGCGCCTGTTTCTGCATATCGGCGCCTACTGGCTGATGTGGAGTTTGCGCTCCCTGATGCCGCGTCGGTCGCGCTGGCGTGGTATCCAGTTC
GATACCCTGCGCCTGCGCCTGATCAAACTCGCCGTCCGCCTCGAAACCCTGAAACGCTCGATCCGCCTGCATCTGCCGCGCTCAATGCCGGACCAGGGAA
TCCTCGGCTATGCCCCGGCCAGACTGCCCAGGCTGGTCAGCTGATCCCAGGGGCGTTTGCCCCGTCCGGCCCCGATCTCTGCCACATCCAACGCCAACAC
ATCGTAGAGCCCACCAAAAACAGCTAGGTTCGGCCGGTAAGCCATCACGCCCGCACGATGTCAGGTTGCACAACCCGCCTGAGAAAAATCAGAGGAAATG
CGCTCGCGAATAATCCCG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1347 bp448 aa981444+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPATVETTPCLPGLSPVAGKPVLARFDGEMLSSDGGLLALREVEKRLGIAAQLAACIDDPRTPEGVQHGLDEIIRFRMLMIAAGYEDGNDADRLRNDPMF
KLAMERLPEAGDLCSQATVSRTENLPGPRALLRMGLAMVEHYCASFRTIPNRIVLDIDDTFDAAHGAQQLCLFNAHHNEYGSQPIVVFDGDGRMLAAVLR
PACRPKGAQIVKWLRRLIDAIRGHWPRTAIMLRGDSHFCTPAVLRFCRARRLDYIFGVARPRRCANTSSRWRPAPRRARNRLRERKSGGSKSSMMAQQAG
TASSGSSPASRPGRWGAIPASSSPA*RLVPRAPCIKRSTARAARPRTISKAWKTHLAADRTSCSRASANQMRLFLHIGAYWLMWSLRSLMPRRSRWRGIQ
FDTLRLRLIKLAVRLETLKRSIRLHLPRSMPDQGILGYAPARLPRLVS

 

Blast result :
Comments
ISAcr5 is 61% aa similar to ISRssp8. There is a STOP codon in phase, the transposase is probably not active.
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Magnuson,T. and Richardson,P. (2006) Direct submission GenBank.