IS1Sa

  • Family IS1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB001339 ND Synechocystis sp.
Synechocystis sp. PCC6803
DNA section
IS Length : 802 bp

Ends


IR Length : 16/22

IRL : GAGAATTTCAAATAGATAATGACGTAGGATGATAGGGAATATTGGGTAAT
IRR : GAGAGTCTCAAGAAAGTAATGAAAGGCGCCAGTTGCGTAATAGTTCTATG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GAGAATTTCAAATAGATAATGACGTAGGATGATAGGGAATATTGGGTAATGAGCTTTTGCAAAGATGTCTACACACTGTCACTGTCCTCAATGTGGTCAT
GGGAATGTCGTCAAAAATGGCTTTGTCAAGGGGAAGCAACGATTCAAATGTAAGAGGTGCCAATATAAATTTACCAATCTCTCCAAGGAACGGGGTAAAC
TTCTCTGGATGAAACTAGAAGCGGTCTTATTGTACATGGGTGGTATGTCAATGAATGCCACAGCAAAACTCTTGGGAGTTTCCACTCAGTCACTGCTCAA
CTGGATTAGGGATTTTGGCGAAGCAAACTACGAGAAACCGGCTCCAGACTCAGCGATCGTAGTGGAATTAGACGAACTGTGGCATTTTCTTCAGGCCAAA
AAAACAAGTTATGGCTCTGGAAGGCATATGACCGTGTTACTGGGAGACTCATCGACTGGGAACTGGGAAATCGTGATAGTCAAACCCTGAGTCGTTTGCT
GGAGAGACTGGCAAAGTGGAAAGTGACAGTGTCCTGTACCGATGACTGGAGGCCCTATCAACAACTGCTGGATGAACATCCTGATGCGTTTCATGGGATT
AGCAAAAGAGAAACAGTAGGAATTGAGAGAAACAACTCAGATAATCGCCATTGGCTTGCTAGATTTCATCGCCCCACGAAAGTTATCTCCAGGTCAGCCC
ACATGGTTAATATCACCATGGCAATATTTGCCAAATTCAGGGTCAATGGTAACATAGAACTATTACGCAACTGGCGCCTTTCATTACTTTCTTGAGACTC
TC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
426 bp141 aa65490+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSTHCHCPQCGHGNVVKNGFVKGKQRFKCKRCQYKFTNLSKERGKLLWMKLEAVLLYMGGMSMNATAKLLGVSTQSLLNWIRDFGEANYEKPAPDSAIVV
ELDELWHFLQAKKTSYGSGRHMTVLLGDSSTGNWEIVIVKPDA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
486 bp161 aa310795+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GFWRSKLRETGSRLSDRSGIRRTVAFSSGQKNKLWLWKAYDRVTGRLIDWELGNRDSQTLSRLLERLAKWKVTVSCTDDWRPYQQLLDEHPDAFHGISKR
ETVGIERNNSDNRHWLARFHRPTKVISRSAHMVNITMAIFAKFRVNGNIELLRNWRLSLLS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
731 bp243 aa65795+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTHCHCPQCGHGNVVKNGFVKGKQRFKCKRCQYKFTNLSKERGKLLWMKLEAVLLYMGGMSMNATAKLLGVSTQSLLNWIRDFGEANYEKPAPDSAIVV
ELDELWHFLQAKKNKLWLWKAYDRVTGRLIDWELGNRDSQTLSRLLERLAKWKVTVYCTDDWRPYQQLLDEHPDAFHGISKRETVGIESKNSDNRHWFAR
FHRPTKVVFRSPYMVDITMAIFAEFRVNGNIELLRNWRLSLLS

 

Blast result :
Comments
See Synechocystis sp. Genome (cf. genome section in ISDatabase).
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
IS1Sa is 57% aa similar to ISCaa1.
References
1] Kaneko,T., Sato,S., Kotani,H., Tanaka,A., Asamizu,E., Nakamura,Y., Miyajima,N., Hirosawa,M., Sugiura,M., Sasamoto,S., Kimura,T., Hosouchi,T., Matsuno,A., Muraki,A., Nakazaki,N., Naruo,K., Okumura,S., Shimpo,S., Takeuchi,C., Wada,T., Watanabe,A., Yamada,M., Yasuda,M. and Tabata,S.(1996) DNA Res. 3 (3), 109-136