ISDre4
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009253 | ND | Desulfotomaculum reducens | Desulfotomaculum reducens MI-1 |
DNA section
IS Length : 1695 bp
Ends
IR Length : 21/26
IRL : CCTGAATCCGTAAAGTAATTTTCTGCCTCACCCCATAATTTATAGGGAAA
IRR : CCTGAATCCGTGAGTTACCTATTCTGTAACCTTATCTTTCAGTAAAATAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCTTTTAGG | TATTA | TCTAACAAAT | 5 |
| AAAGTTAATT | AATAA | GTTAAAAAAC | 5 |
| TACGTATTAT | AATTCT | GCAAAAAAAT | 6 |
| TAACTACAAT | ATTAA | TTCTGATCTT | 5 |
| GGCTAAAAAA | TTAAT | ATTATGGACT | 5 |
| TGTCTTTTAC | AATTCAT | GCTTGTTATT | 7 |
DNA sequence
CCTGAATCCGTAAAGTAATTTTCTGCCTCACCCCATAATTTATAGGGAAATGAAGGAATATGTAGAAATATACATCTAATCGATATTTCTAATCTTCACC
TCAGGGGTGCAACATGAAAAAGATAAGAAACAAGAAAATCAAAACCAAAAACAGAGACGATGATAGGCTCACTATCTTTGAAATTGAACAAGGTGACGAA
GAGCTAACCACCCATTCGGGCTTAGCTCTTATCGGAGCCTTACTGACTAATACCAAGATAAAAACACGACTGAACAATACAATGTCTGCTGAACAAAAGA
AACCCCATATTTCTAATGGAAGTGTTGGTATTGCCTACATTGGCTTGTTATGCCAAGGCAAGAGTGATTTTGACCACATTGAACCCTTTCGAGAAGATAA
TTTCTTTGCAATCTCGTTAAACATCAAAGAGACACCGTCAAGCCCAACCCTTCGCCAACGATTAGATATGGTTGCTAAAGATAAGCAATGGAATAATATC
CTGTTGGAAGAATCTGCGGGGCTAATCAAAAAAACAAATGCACCACTAACCCCAGTGTACCTGGGTCAAGAAAATAGAGCTTATCTTCCACTAGATATTG
ATGTGTCTCCCTTTGATAACTCCAACACCAAAAAAGAAGGGGTCTCCCGCACCTACAAAGGCACCGATGGTTACGCTCCCATCTTTGCATATCTAGCAAA
GGAAGGTTATTGCGTAAATACTGAGCTACGCCAAGGGAGTGAACATTGCCAAAAGAACACCTCAGAATTCGTTGCCGAAAGCATTCGCTATGCCAGAAAA
ATTACCCAGTTGCCTTTGCTTTTAAGGATGGACTCAGGCAATGACAGCGCTAGTAATATTGAAATTTGCTTAAATGATGATACTAAGGCTGATTTCATTA
TTAAGCGAAACCTTCGCAAAGAAACCCCTGAAGGGTGGCTACTATTGGCAAAGAACAATAAAGATATTTACTGCCAGGAACGTGAAGGTAAAAAAGTCTA
CTATGGTTCCATGATGAAATACAAAAAGGAGCTGAAAAGAGAAATCAGGGTAGTTTACAAAATTACCGAAAGAACCTTTGGTAAAGATGGCCAGATATTT
CTCGTACCCCAGGTGGAGGCAGAAACCTATTGGACCTCATTGCCAGACCCTCCACATGTAATCGAAAGACTATACCACGAACATGGCACCAGTGAGCAGT
TTCACAGCGAACTTAAGACAGACCTGGATTTAGAAAGACTGCCCTCTGGCAAATTTGACACTAACAACCTAATACTACATTTTGGAGTAGTGGCCTACAA
TCTGTTGCGCATGATAGGACAATCAACAACTAGAATGCAGCATGTGCCCTTGCGAAAGCAGGCAGAACGCCGTAGGATTAGGACGGTGATTCAGAACTTA
ATCACGTTGGCATCACGGTTAGTTTCACATGCAAGAAAGAAGAAATTACGATTTGGAAAGCACAGTCCCTGGTTTGAAACATTTAAACAAGCTTACTCTG
TGTGTTTAGCAAGATAGAAATATCCACAGAACGATAAAACTTTGCACTAAAAGGAGTGCTTAGATTTTTATTGCCAAATTTAAGCTCAATACTATTGAGA
GATTCATTAAATGCTAGTGGCAATTCTTAAAATATAAATTTTTACATATTTTACTGAAAGATAAGGTTACAGAATAGGTAACTCACGGATTCAGG
TCAGGGGTGCAACATGAAAAAGATAAGAAACAAGAAAATCAAAACCAAAAACAGAGACGATGATAGGCTCACTATCTTTGAAATTGAACAAGGTGACGAA
GAGCTAACCACCCATTCGGGCTTAGCTCTTATCGGAGCCTTACTGACTAATACCAAGATAAAAACACGACTGAACAATACAATGTCTGCTGAACAAAAGA
AACCCCATATTTCTAATGGAAGTGTTGGTATTGCCTACATTGGCTTGTTATGCCAAGGCAAGAGTGATTTTGACCACATTGAACCCTTTCGAGAAGATAA
TTTCTTTGCAATCTCGTTAAACATCAAAGAGACACCGTCAAGCCCAACCCTTCGCCAACGATTAGATATGGTTGCTAAAGATAAGCAATGGAATAATATC
CTGTTGGAAGAATCTGCGGGGCTAATCAAAAAAACAAATGCACCACTAACCCCAGTGTACCTGGGTCAAGAAAATAGAGCTTATCTTCCACTAGATATTG
ATGTGTCTCCCTTTGATAACTCCAACACCAAAAAAGAAGGGGTCTCCCGCACCTACAAAGGCACCGATGGTTACGCTCCCATCTTTGCATATCTAGCAAA
GGAAGGTTATTGCGTAAATACTGAGCTACGCCAAGGGAGTGAACATTGCCAAAAGAACACCTCAGAATTCGTTGCCGAAAGCATTCGCTATGCCAGAAAA
ATTACCCAGTTGCCTTTGCTTTTAAGGATGGACTCAGGCAATGACAGCGCTAGTAATATTGAAATTTGCTTAAATGATGATACTAAGGCTGATTTCATTA
TTAAGCGAAACCTTCGCAAAGAAACCCCTGAAGGGTGGCTACTATTGGCAAAGAACAATAAAGATATTTACTGCCAGGAACGTGAAGGTAAAAAAGTCTA
CTATGGTTCCATGATGAAATACAAAAAGGAGCTGAAAAGAGAAATCAGGGTAGTTTACAAAATTACCGAAAGAACCTTTGGTAAAGATGGCCAGATATTT
CTCGTACCCCAGGTGGAGGCAGAAACCTATTGGACCTCATTGCCAGACCCTCCACATGTAATCGAAAGACTATACCACGAACATGGCACCAGTGAGCAGT
TTCACAGCGAACTTAAGACAGACCTGGATTTAGAAAGACTGCCCTCTGGCAAATTTGACACTAACAACCTAATACTACATTTTGGAGTAGTGGCCTACAA
TCTGTTGCGCATGATAGGACAATCAACAACTAGAATGCAGCATGTGCCCTTGCGAAAGCAGGCAGAACGCCGTAGGATTAGGACGGTGATTCAGAACTTA
ATCACGTTGGCATCACGGTTAGTTTCACATGCAAGAAAGAAGAAATTACGATTTGGAAAGCACAGTCCCTGGTTTGAAACATTTAAACAAGCTTACTCTG
TGTGTTTAGCAAGATAGAAATATCCACAGAACGATAAAACTTTGCACTAAAAGGAGTGCTTAGATTTTTATTGCCAAATTTAAGCTCAATACTATTGAGA
GATTCATTAAATGCTAGTGGCAATTCTTAAAATATAAATTTTTACATATTTTACTGAAAGATAAGGTTACAGAATAGGTAACTCACGGATTCAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 467 aa | 114 | 1517 | + | No |
Chemistry : DDE
ORF sequence :
MKKIRNKKIKTKNRDDDRLTIFEIEQGDEELTTHSGLALIGALLTNTKIKTRLNNTMSAEQKKPHISNGSVGIAYIGLLCQGKSDFDHIEPFREDNFFAI
SLNIKETPSSPTLRQRLDMVAKDKQWNNILLEESAGLIKKTNAPLTPVYLGQENRAYLPLDIDVSPFDNSNTKKEGVSRTYKGTDGYAPIFAYLAKEGYC
VNTELRQGSEHCQKNTSEFVAESIRYARKITQLPLLLRMDSGNDSASNIEICLNDDTKADFIIKRNLRKETPEGWLLLAKNNKDIYCQEREGKKVYYGSM
MKYKKELKREIRVVYKITERTFGKDGQIFLVPQVEAETYWTSLPDPPHVIERLYHEHGTSEQFHSELKTDLDLERLPSGKFDTNNLILHFGVVAYNLLRM
IGQSTTRMQHVPLRKQAERRRIRTVIQNLITLASRLVSHARKKKLRFGKHSPWFETFKQAYSVCLAR
SLNIKETPSSPTLRQRLDMVAKDKQWNNILLEESAGLIKKTNAPLTPVYLGQENRAYLPLDIDVSPFDNSNTKKEGVSRTYKGTDGYAPIFAYLAKEGYC
VNTELRQGSEHCQKNTSEFVAESIRYARKITQLPLLLRMDSGNDSASNIEICLNDDTKADFIIKRNLRKETPEGWLLLAKNNKDIYCQEREGKKVYYGSM
MKYKKELKREIRVVYKITERTFGKDGQIFLVPQVEAETYWTSLPDPPHVIERLYHEHGTSEQFHSELKTDLDLERLPSGKFDTNNLILHFGVVAYNLLRM
IGQSTTRMQHVPLRKQAERRRIRTVIQNLITLASRLVSHARKKKLRFGKHSPWFETFKQAYSVCLAR
Blast result :
Comments
ISDre4 is 70% aa similar to ISHmo1.
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Tebo,B.M. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Tebo,B.M. and Richardson,P. (2007) Direct submission GenBank.