ISfinder Mirror Site

ISPlpa1

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ABCS01000060	ND	Plesiocystis pacifica	Plesiocystis pacifica SIR-1

DNA section

IS Length : 1849 bp

Ends

IR Length : 15/19

IRL : CCTGAAGTAAGCGCGTTGGTCAGTCCGACGCGTGAGTGAGATCCAAAACC
IRR : CCAAAAATAAGCGCGTTCGGAGCAAAGCGAGCCCCCCGCACGAGTCCGAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGAGACCAA	GCTAGC	ACCGAGGTCG	6
TGCGCTCTGC	GCTAGC	CATGGGATGC	6
TGAAGGGAGA	ACTAGC	GTGGGGACTG	6

DNA sequence

CCTGAAGTAAGCGCGTTGGTCAGTCCGACGCGTGAGTGAGATCCAAAACCATAGCTGGAGTCCCGCAGAGAGCAGTTGTCAGAGGAAGTGCCAACCACCT
CAACAGCCAACCTCGAGGACTCCAAGTGAAGAAGCCTACACCGCTCCCCCGCGCCAGTCACGTCAACTCCGGGACGTCGATTCCTCACGAGGAGCTGCCC
TGGCCCAGCCGCCCAGGGCCGGTCCTGCGGCATCACAAGATCCACCTCGAGCATGACGCTCGACCCGATATCACGAGCCTTGGCGGGCTGGCATTGCCGC
TGGCATTGCTGCGGCGCTTGAAGGCGGCGCAGACCTTGGACGCCAACGTCGATGTATTCAAGCGCTACCTCCCCTACCACGAGTCCGATCACATCATCTG
TCAGGCCTTGATGCTCTACGCAGGCGGCTCATGTCTGGAAGACATGGCGATGCTGCAGCAAGACCAGGCGTTGCTCAAGATCCTGGGCGCTGAACGCACG
CCTGACCCGACGACCTCGGGAGATTTCCTCAGGCGCTTCGTCCCGAGGGGCAAACTCGAGAGCCTGCGTGCGGGCATCGATGAGGTGCAAGAGACGATGT
GGAAGAATCCGAAGCGCTGGTCGTGGTCGCGCAAGCGCAAGCGCAAACCGCTCGCGGTCTTGCATCTCGATGGTCATCTCGAGCAGACGTCTTCGACCCG
CGAGGGCGCAGATTTCAACTACAAGGGCAAGTGGTCCTACAGCGTCTTGGTCGCATCTTTGGCCGATGGCGAGTGTGTCGGGCTGCGGCTTCGTGGCGGG
AATGTCCGCTCCAGTGATGGGGCTGCCGAGCTCCTCGATGACGTACTCCCGCGTCTGCTCAAACAATTCGAGACCGTCCTCGTGCTCGCAGACAGCGACT
ACGATCGCGCCGATCTTCGTCATGCCTGCGAAGGACATGGCTGCTACTTCGCGTTCGTGGGGCGGGAGACGCCTGATCGGCCAGCAATCGCCGAGCAACC
CCGAGTGTGGCGCTCCTTCCAAACTCGAGCCAAGCGAGCAGCGGCCGAGCGCGAAGCCAAGCCAAGCTTCAAGCGTCGAAAGAAGAAGCGAAACCGTCGC
AAGCGCCGAGCTCGGCAGCGAGGCTACAAGGAGCTGAAGCTTGCCAGGCAAGCGGTCGCTGAGACTGAAGGTCCCGGCGGCACACGGATCATCATTCGCC
GACAAATACTCGACGTCTCCAAGGGCAAGTCGCCGCAGCGCGAGATGTGGAAAGAGGTCCGGTATCGCTACGTGGTCACGAATCTCCCGAGCAGCTGGTC
GGCTGAAGATGTGATCGACGAGACTTACAAGCGCTGTGATCAAGAGAACATCATGGCTGGTCTTCACACGGGGATCGCCGCGTGGAGGATGCCGGTCGCT
CAGCTGCGGGGAAACGCTGCGTGGCTGGAGATCGCCCGACTGGCTTGGAACCTCGGCAAATGGACAGCTCAGCTCGCGCTGCCCAAAGAAGTCACCCGGT
GGGAATGGAAGCGCTTTCGCCGAGCCTTCGTCGACATCCCAGCCCAGGTCATACGCACAGCTCGCCAGCTGCGGGTCCGGGTGCTTGGGACACATCGCTA
CATCAACGAGTTTCTCTCCGCCCACGCCTGCCTGCAGGTCTGAACTTCAGTGACATCAGCGCTACGAGGAGCGTCGGCTTCGGGACACGTGCGCCCTCAC
GCCCAGTCCGAGTCGGTCGCGCGGCTGATCGTGCTGCTGATTGAGCTCGAGAGGCTCGAACTCGACGTCAAGGTGGTCGAACTCGAGCTCGTCGACCTAC
TCGGACTCGTGCGGGGGGCTCGCTTTGCTCCGAACGCGCTTATTTTTGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1338 bp	445 aa	306	1643	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLRRLKAAQTLDANVDVFKRYLPYHESDHIICQALMLYAGGSCLEDMAMLQQDQALLKILGAERTPDPTTSGDFLRRFVPRGKLESLRAGIDEVQETMWK
NPKRWSWSRKRKRKPLAVLHLDGHLEQTSSTREGADFNYKGKWSYSVLVASLADGECVGLRLRGGNVRSSDGAAELLDDVLPRLLKQFETVLVLADSDYD
RADLRHACEGHGCYFAFVGRETPDRPAIAEQPRVWRSFQTRAKRAAAEREAKPSFKRRKKKRNRRKRRARQRGYKELKLARQAVAETEGPGGTRIIIRRQ
ILDVSKGKSPQREMWKEVRYRYVVTNLPSSWSAEDVIDETYKRCDQENIMAGLHTGIAAWRMPVAQLRGNAAWLEIARLAWNLGKWTAQLALPKEVTRWE
WKRFRRAFVDIPAQVIRTARQLRVRVLGTHRYINEFLSAHACLQV

Blast result :

Comments

ISPlpa1 is 39% aa similar to ISLsp3.

References

1] ISfinder annotation (2009)
2] Shimkets,L., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.