ISfinder Mirror Site

ISAcma44

Family IS4
Group IS10

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 plasmid pREB7

DNA section

IS Length : 1304 bp

Ends

IR Length : 17/19

IRL : CTTATTGGTGCAGAAAAGTCGTGAACACCATGCTAGGGAAGGATTACTAA
IRR : CTTAGTGGTGCAGAAAACTTTGAGTAGGCTGAAAAGCTTAAATAACAGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTTGTAAAATA	GATTCCAAC	TATTATCAGA	9

DNA sequence

CTTATTGGTGCAGAAAAGTCGTGAACACCATGCTAGGGAAGGATTACTAAGCCATTAGAGGGATGGAGTGAGACCTACAATGTGAGGCTTAATTACCTCA
CTTTCCTTCAAGCACATGTCAACTCCTCTGCTGTACCGTCAACTGCAAACTCAACTGAGCCAATGGATCAGTCCCAAGGATCATCGTCACCTGACGGTCT
TTTCTGAAAATATTGCCGCGATTCTACAAGCCCAGAGTGGCTGCATGAGCCATTGGCTGTCGTACTTGAGTCATCGGTCCTGCCAAGCTCGTAGTCAGAT
GGAACGCCTGAGCTATTTTCTTCATAATCCCAGAATCCTCTCAGAGACTTTCTATGCACCATTACTCAAGCAATTTCTTCATGCTTGGGAAGGAATGTCA
ATGACATTAACCCTAGATACGAGCATGCTTTGGGACACCTATTGCTTGATAGAGGTCTGTTTAGCGTGGGGTGGACGTTCGTTCCCGTTAGCTCAAAAAG
TCATGGAACATGGTAGTGCCACGGTTGCTTTTGTAGACTATTGCAGTGTTTTATCGATGACTCAAGGGGTGTTACCTCCTCGTTGCCACATCACGTTATT
GGCAGACCGAGGCTTTGAGCACGGTGAGTTGATTCGCTGGCTCCGCTCATCTGAGTGGTCTTGGGCCATTCGAGCCAAATCTGATTTGCAGATTACCTTA
GCTAATGGTCGCAGTAAACCAGTATCCAAGCTACTCCCTGAAGTTGAGCAAGCGTCACTCTTTCGGGATGTCATGATCCTTGAGGATATTCACTGTCACT
TGGCCACAGCTTCAGTCTCCACAACACAGGAGGCTTGGGCGGTGATTACGGATACGCCGCCGTCATTGCAAACCTTTGCCGTTTATGGGCAACGCTTTGG
CGGGATTGAACCCCATTTCAAGGATTATAAGTCGGCAGCGTTTGAGGTACCTCGCTCTCACATCAGAGATACTGCAGCCTTAGAACGCTTACTGATGTTA
CTCGCTGCTGCAACTCTCATCGCGATCTCTGTAGCCTTTCAGGTGATCGCTCAAGATGCGCTCAAAACCATTGATTGGCATACTCACAGGGGATTAAGCT
TTCTGCAAATCGGTTTGCGGCAGATCAATCAGCTTTGCTACCTAAGATTACCCCTACCCCCGTTAGCAATGTTGCCCCGACAAAATCCGCCTCCTGCTTA
TGCCTCCCTAAAGAAACGAGCTGCTATGAAGACTTGCATTGAATTCGCTAAGGTAACTGTTATTTAAGCTTTTCAGCCTACTCAAAGTTTTCTGCACCAC
TAAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1152 bp	383 aa	116	1267	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTPLLYRQLQTQLSQWISPKDHRHLTVFSENIAAILQAQSGCMSHWLSYLSHRSCQARSQMERLSYFLHNPRILSETFYAPLLKQFLHAWEGMSMTLTL
DTSMLWDTYCLIEVCLAWGGRSFPLAQKVMEHGSATVAFVDYCSVLSMTQGVLPPRCHITLLADRGFEHGELIRWLRSSEWSWAIRAKSDLQITLANGRS
KPVSKLLPEVEQASLFRDVMILEDIHCHLATASVSTTQEAWAVITDTPPSLQTFAVYGQRFGGIEPHFKDYKSAAFEVPRSHIRDTAALERLLMLLAAAT
LIAISVAFQVIAQDALKTIDWHTHRGLSFLQIGLRQINQLCYLRLPLPPLAMLPRQNPPPAYASLKKRAAMKTCIEFAKVTVI

Blast result :

Comments

ISAcma44 is 44% aa similar to ISMmg3.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)