ISApr10

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Alpha proteobacterium
Alpha proteobacterium BAL199
DNA section
IS Length : 1366 bp

Ends


IR Length : 12/18

IRL : GGGGTGTCGAATCCGCGAAGGAGAGCAACGCCATGAAGAAAGCTACCAGA
IRR : GGGGATGTCCCTCGCCGTGTTGAAATTTGCTCGGCAGGCGCTGTTGGTTG

Insertion site


Left flankDirect repeatRight flankDR Length
AGCCATTAGGCTCGGGTGAAGGCAAC0
GCAGGAGCGCTTCGTCCGGGCGCACG0

DNA sequence

GGGGTGTCGAATCCGCGAAGGAGAGCAACGCCATGAAGAAAGCTACCAGATCTGCCGGCGTGGTGCCGGCGGCAATCGTCGATGAGGCTTGGCAGGAAGT
CGGAGCCAGCTTCGATCGTTTCTGCCTGACGGCGGGCCTGGCGACTTTGGCGACGATGATGGATGAGGAGGCGGCCGAGCTGTGCGGCCCGCGCTACGGC
CGGGCTGCTGGGCGAGACGGGTATCGCTGGGGCAAGACCAAGGGGAAGGTCGGCTTCCACGGCGGCAAGGTCGAGATCGATCGTCCTCGCGTTCGTGCTC
GCGACGGCGGCGAGCTGGCGCTGCCGAGCTGGGAGGCGGCGCAGTCGGAAGACCTGCTCGGCAAGTGGGCGCTGAACCTGATGCTGATCAACGTCTCGAC
CCGCAAGTTCGGCCGCGCCGTCCGCCTGCCGGGCGGCGACATCCCGGCGGCCCCCGGGTCGGGAGTGTCGAAGTCGGCGGTGTCGCGGCGGTTCGTGGCG
CTGTCGGCGGAACGCATGAAGGAATGGATGGCCGCCGACTTGTCCGCCCTGGACCTGCTGGCGATCCAGATCGACGGCATCCACATCGAGGAAGACCTGA
TGCTGCTGGCCGCCGTCGGCATCGACGGCATCGGCGACAAGCATCCGCTCGGCGTGATCGAGGGGGCCAGCGAGAACGCCGCCGTGGCCCAGGCGCTGCT
CGACAACCTGGTCGGCCGCGGCCTCGATCCCGCGGTCTGCCGACTGTTCATCATCGACGGCGCCAAGGCGCTGTCGAAGGCGATTCGCAACACCTTCGGC
CGCGACACCCCGATCCAGCGCTGCCAGGTCCACAAGGCCCGCAACATCGCAGAGCGCCTGCCCAAGTCCTTGCAGGCCGGCGTCCGCAAGGCGCTGCGTC
AGGCCTGGGAACTGGACGACGCCGACAAGGCCGAGAAGCTGATCCGCAACCTCGCCCGTCGCCTGGAGCGCGACGCCCCGGGCGTGTCGGCCAGCATCCT
GGAGGGCCTCGACGAGATCCTCACCGTCACCCGCCTCGGTCTGCCAGTCGAACTACGCCGGTCGCTCGCCTGCACCAACATCATCGAGAACATGAACGGA
ACCGTCCGCCGGGTCTGCCGCAACGTCAAACGCTGGCAGGACGCGGCCATGGCGCTGCGCTGGACCGCCGCTGCCATGCTCGAGGCCGCCAAGGGCTTCC
GCCGCCTCAAGGCCCACAAGCAACTCCCGAGCCTGCGCCGAGCCCTGGCGGCCCACCAGGACAGGAACGCGATCAACCACGACCTTGAACAGCAGGCGGT
CGCCGCATAGCATCGTCAACCAACAGCGCCTGCCGAGCAAATTTCAACACGGCGAGGGACATCCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1278 bp425 aa331310+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKATRSAGVVPAAIVDEAWQEVGASFDRFCLTAGLATLATMMDEEAAELCGPRYGRAAGRDGYRWGKTKGKVGFHGGKVEIDRPRVRARDGGELALPSW
EAAQSEDLLGKWALNLMLINVSTRKFGRAVRLPGGDIPAAPGSGVSKSAVSRRFVALSAERMKEWMAADLSALDLLAIQIDGIHIEEDLMLLAAVGIDGI
GDKHPLGVIEGASENAAVAQALLDNLVGRGLDPAVCRLFIIDGAKALSKAIRNTFGRDTPIQRCQVHKARNIAERLPKSLQAGVRKALRQAWELDDADKA
EKLIRNLARRLERDAPGVSASILEGLDEILTVTRLGLPVELRRSLACTNIIENMNGTVRRVCRNVKRWQDAAMALRWTAAAMLEAAKGFRRLKAHKQLPS
LRRALAAHQDRNAINHDLEQQAVAA

 

Blast result :
Comments
ISApr10 is 55% aa similar to IS931.
Genome in progress : Accession number : NZ_ABHC01000016.
References
1] ISfinder annotation (2009)
2] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.