ISAcma46

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB5
DNA section
IS Length : 1864 bp

Ends


IR Length : 31/32

IRL : GGGGTAATTGAAGGGTCGACACTGTTGGCGAAATATTCCGCAATGATCTA
IRR : GGGGTAATTGAAGGGTTGACACTGTTGGCGAATTCCAAGAAGTCACGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
AGCTTAACTTTAATATGAGT0

DNA sequence

GGGGTAATTGAAGGGTCGACACTGTTGGCGAAATATTCCGCAATGATCTAAGACGGCTGTAAAAAGTTTCTAACCATTGATGCAGCGAGCCTTTCGTGGA
TTGAGGTATGTTACTGCTAAAACTTAAAATCAATCACGCTCACTCTGGAGGCTCATTGTTATATCTATGCGCCCGACAGACTTGTTGGACATCCCTAGTG
AAACCCTTGAAGTAGCTAGGGCTGCCTTTCCTAATGGCAACCTGTATATGCAACTACGTGATGAACTTGGCATTATCTATCACGATCACGATTTTGCTGA
TCTGTTTCCAAAGAAAGGACAGCCAGCTTTATCCCCCTGGCAGCTTGTGTTAGTTTGCATCATGCAATTTCTTGAGAATCTTTCAGACCGTCAAGCGGCT
GATGCCGTTCGAGCCCGGATAGATTGGAAGTATGCCCTGAGCCTACCGCTGAAAGACTCTGGATTTAATTATTCTATTTTGAGTGAATTCCGCAAGCGTC
TAATCGAGGGAGGCAAGGAACAACGGCTATTTGATATCTTTCTTGAGCACCTTCAATCCAAAGGGTTTCTCAAAGAACACCATCAACAACGAACGGATGC
GACTCATATCTTAGCGGCGATCCGAACCCTCAATCGGTTGGAGTTATTTGGTGAAACCTTTCGGGCTGCTCTCAACAGTTTAGTCGTTGCAGATCCACAG
TGGTTAAGCGAACACATGCAAGAAGACTGGTTTGATCGCTATGGTCGACGAATAGAGAATTATCGCTTGCCTAAACCCGATAGCGAACGCAAAGCCCTGG
GCAGTACTATCGGTCAAGATGGTTTGGCACTGCTTGATGCGATATACCACCCAAGCTCACCACCCTGGTTAAAGCATCTACCAGCTGTAGAAACTTTGCG
GCAAGTCTGGCTCCAGCGATTCTACCCGCCCAATGCAGACGGCACAGTCGAATGGCGAAAAGTGAAGGATATGCCCCCTTCGGATGTATCCATTCATTCT
CCTTACGACATAGAAGCCCGCTACAGCAATAAAAGAAGTATGGATTGGGTGGGCTATAAAGTTCATTTCACTGAAGTGTGTGATCACGATAATCCCCGCC
TCATTACTCATGTGCATACTACCCGTGCAACCGTCCCAGATGATCAAGTTGTTGAACCCATTCACCTTGCCTTAGCGGAGAAACGATTGTTGCCTAAAGA
GCATTTGCTAGATGGAGGCTATCTTTCTGCTGAGCATTTAGTGAATAGTGAAGCAGAATATGACATGGACCTTGTTGGCCCCGTGCGTCAAAACCACAGC
TGGCAGGCAAAGGCAGGGAATGGGTTTGATGCCAGCGTCTTCCAAATTAACTGGGACCAACAGAAGGTTGTCTGTCCTCAAGGTCACTCCAGTACAAAGT
GGTTGCCTGGACAGGATATTCATGGAAAGGATGTCATTCGAGCACGGTTCCTAGGGACAACTTGTCGAAGTTGTCCCGTACGATCTCAATGTACGAAATC
AAAGACCCAGCCTAGAGAGCTAACCTTTCGTCCCCGTGAATTGCATCAGGCCCTTAACGAACGGCGTCAGGTGCAACGAACGACTGAGTTCAAGGAAAAA
TATAGCCTGCGGGCTGGTGTTGAGAGTACCCATTCACAGGGAATACGGCGATTTGGCATGCGCCAGTCAAGGTATATCGGGTTAGCGAAGAGCCATCTAC
AGAATGTTTTCTGTGCCATTGCTCTCAATTTTGTCCGCTTAGACGCTTGGTTTAATGATGTTCCACTAGCAAAAACCCGTGTATCTCTTTTCAAGAGGAT
ACTTCAGCCATTACCAGCGTGACTTCTTGGAATTCGCCAACAGTGTCAACCCTTCAATTACCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1656 bp551 aa1671822+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRPTDLLDIPSETLEVARAAFPNGNLYMQLRDELGIIYHDHDFADLFPKKGQPALSPWQLVLVCIMQFLENLSDRQAADAVRARIDWKYALSLPLKDSGF
NYSILSEFRKRLIEGGKEQRLFDIFLEHLQSKGFLKEHHQQRTDATHILAAIRTLNRLELFGETFRAALNSLVVADPQWLSEHMQEDWFDRYGRRIENYR
LPKPDSERKALGSTIGQDGLALLDAIYHPSSPPWLKHLPAVETLRQVWLQRFYPPNADGTVEWRKVKDMPPSDVSIHSPYDIEARYSNKRSMDWVGYKVH
FTEVCDHDNPRLITHVHTTRATVPDDQVVEPIHLALAEKRLLPKEHLLDGGYLSAEHLVNSEAEYDMDLVGPVRQNHSWQAKAGNGFDASVFQINWDQQK
VVCPQGHSSTKWLPGQDIHGKDVIRARFLGTTCRSCPVRSQCTKSKTQPRELTFRPRELHQALNERRQVQRTTEFKEKYSLRAGVESTHSQGIRRFGMRQ
SRYIGLAKSHLQNVFCAIALNFVRLDAWFNDVPLAKTRVSLFKRILQPLPA

 

Blast result :
Comments
ISAcma46 is 41% aa similar to ISPlu14.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)