ISAcma45

  • Family IS5
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB6
Acaryochloris marina MBIC11017 plasmid pREB3
DNA section
IS Length : 1830 bp

Ends


IR Length : 16/17

IRL : GACACTGTTGGCGAAATATTCCGCAATGATCTAAGTCGGCTGAAAATAGC
IRR : GACACTGTTGGCGAATTCCAAGAAGTCACGCTGGTAATGGCTGAAGTATC

Insertion site


Left flankDirect repeatRight flankDR Length
CTGGTGTCAGCAACCCTTCAATTACCCC6
GGGGTAATTGAAGGGTTGCTGACACCAG6
ATTGAAGGGTCAGGGTTGCTGA0
TCAGCGACCCTAGGGTCGCTGA0
ATTGAAGGGTTAACCCTTCAAT0

DNA sequence

GACACTGTTGGCGAAATATTCCGCAATGATCTAAGTCGGCTGAAAATAGCTTCTAACACTTGATGCAGTAAGCCTTTTGTGGATTGAGGTATGGTACTGC
TAAAACTCAAACTCAATCACGCTCACTCTGGAGGCTCATTGTTATGTCTATGCGCCCGACAGACTTGTTGGACATCCCCAGTGAAACCATTGAAGTAGCT
AGGGCTGCCTTTCCTGATGGCAATCTGTATATGCAACTACGTGATGAACTTGGCATTATCTATCACGATCACGATTTTGCTGATCTGTTTCCGAAGAAAG
GACAACCAGCTCTATCCCCCTGGCAGCTTGTGCTAGTTTGCATCATGCAATTTCTTGAGAATCTTTCAGACCGTCAAGCGGCTGATGCCGTTCGAGCCCG
GATAGATTGGAAATATGCCCTGAGCCTACCGCTGAGAGACTCTGGATTTAATTATTCTGTTTTGAGTGAATTTCGCAAGCGTCTGATCGCGGGTGACAAG
GAACAACAGCTATTTGATAGCTTCCTTGAGCACCTTCAAGCAAAAGGGTTCCTTAAAGAACACCATCAACAACGAACGGATGCGACCCATATCCTAGCGT
CGATCCGAACCCTCAATCGGTTGGAGTTATTTGGTGAAACCTTTCGGGCTGCTCTCAATAGTTTGGTTGTCGCAGATCCACAGTGGTTAAGCGAACACAT
GCACGATGACTGGTTTGATCGCTATGGTCGACGAATAGAGAATTATCGCTTGCCCAAACCCGATAGCGAACGCAAAGCTTTGGGCAGTACTATCGGTCAA
GATGGTTTGACACTGCTTGACGCCATATATGCCCCTAGTTCACCACCGTGGTTAAAGCATCTACCTGCAGTGGAAACTCTGCGACAAGTCTGGCTCCAGC
GATTCTACCCGCCCAATGCAGACGGCACAGTCGAATGGCGAAAAGTGAAGGATATGCCCCCTTCTGATTCATCCATTCATTCTCCTTACGATGTCGAAGC
CCGCTATAGCAATAAAAGAAGTATGGATTGGGTGGGCTATAAAGTTCATTTAACAGAAGTGTGTGATCCTGAAAGTCCACGTCTCATCACTCATGTGCAC
ACCACCAGTGCAACGGTTCCAGATGATCAAGTTGTTGAACCCATTCACCTGGCTCTGGCTAAAAAACAGTTGTTGCCTAAAGAGCATTTGTTAGATGGGG
GTTACCTCTCGGCAGAGCATTTGGTGAATAGCAAAGCAGAATATGACATGGATGTTATTGGTCCAGTGCGTCAAAATCATAGTTGGCAAGCAAAGGCAGG
AAACGGATTTGATTCGAGCTTCTTCCTCATCAGTTGGGACCAACAGAAGGTTGTCTGTCCTCAAGGCCACTTCAGTACGAAATGGTTGCCTGGAAAGGAT
ATTCACGGAAAGGATGTCATTCAAGCAAGGTTCCAGGGGACCACTTGCCGAAGTTGTCCAGTGCGATCTCAATGTACGAAATCAAAGACCCAGCCAAGAG
AGTTGACGTTCCGTCCCCGTGAGTTACATCAAGCCCTTAATGAACGACGTCAGGTACAACGAACTACTGAGTTCAAGGAAAAATATAGCCTGCGGGCTGG
TGTTGAGAGTACCCATTCACAGGGAATACGGCGATTTGGCATGCGCCAATCAAGGTATATCGGGTTAGCGAAGAGCCATCTACAGAATGTTTTCTGTGCC
ATTGCTCTCAATTTTGTCCGCTTAGACGCTTGGTTTAATGATGTTCCACTAGCAAAAACTCGTGTATCTCTTTTCAAGAGGATACTTCAGCCATTACCAG
CGTGACTTCTTGGAATTCGCCAACAGTGTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1662 bp553 aa1441805+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSMRPTDLLDIPSETIEVARAAFPDGNLYMQLRDELGIIYHDHDFADLFPKKGQPALSPWQLVLVCIMQFLENLSDRQAADAVRARIDWKYALSLPLRDS
GFNYSVLSEFRKRLIAGDKEQQLFDSFLEHLQAKGFLKEHHQQRTDATHILASIRTLNRLELFGETFRAALNSLVVADPQWLSEHMHDDWFDRYGRRIEN
YRLPKPDSERKALGSTIGQDGLTLLDAIYAPSSPPWLKHLPAVETLRQVWLQRFYPPNADGTVEWRKVKDMPPSDSSIHSPYDVEARYSNKRSMDWVGYK
VHLTEVCDPESPRLITHVHTTSATVPDDQVVEPIHLALAKKQLLPKEHLLDGGYLSAEHLVNSKAEYDMDVIGPVRQNHSWQAKAGNGFDSSFFLISWDQ
QKVVCPQGHFSTKWLPGKDIHGKDVIQARFQGTTCRSCPVRSQCTKSKTQPRELTFRPRELHQALNERRQVQRTTEFKEKYSLRAGVESTHSQGIRRFGM
RQSRYIGLAKSHLQNVFCAIALNFVRLDAWFNDVPLAKTRVSLFKRILQPLPA

 

Blast result :
Comments
ISAcma45 is 49% aa similar to ISPlu14.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)