ISApr8

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Alpha proteobacterium
Alpha proteobacterium BAL199
DNA section
IS Length : 1667 bp

Ends


IR Length : 13/17

IRL : CCGGATTTCCCAGATGGCGCTGCGTTTTCCTGACGGTGGCGGTCAAATAT
IRR : CCGACATTTCCCATTTGAGCGGCCGAACCGCCCCTGTTTGATGGCAGGAT

Insertion site


Left flankDirect repeatRight flankDR Length
GTCCAGGCGTGTTTCAACTTATTATATTCTTCT0
TCTGCTGCTGCACCTTAATTCAATGTCGACTAC0

DNA sequence

CCGGATTTCCCAGATGGCGCTGCGTTTTCCTGACGGTGGCGGTCAAATATGATATCAAAATCAATGCAGTACTGACGATTTGAGGGGCTGGGTATGGAGA
TGGCGGCGGGTGAAACGGAATCGGCCGATTTGCGGGTCGCTTTTGACCGCCGCCTCAAGCTGGAATTCCACGGCTCCAGGATCACCTCCGATGCCGGACT
GCTCGCCTTCCGGGAACTCGATGATGCTCTCGGTCTGACCGAGATGGCCGGGCAGGTGTTGGCCGACAGCCGGACCGGCAAGAACAACCGCCACACCCTT
ACGGCCCAGTTCCGGCAGTCGGTATTCGGTCGGCTTGCCGGGTACGAAGATGTCAACGACGCCGACCGGCTCGGCCATGATCCGGCGATGCGCTGGATCG
TCGGCGGCAGGGCTGTGACCAAGGAGGCCGCGTCCACCAGCCAGATGGGGCGGTTCGAAACCGAGGTGCTGACTGGCAAGGCGAACCTCACCGCCTTGGC
CGACCTGTCAGGAAAATGGATCGACGCGGTTCATGCCCGCCGTCCCACCAACGTCGTGGTGCTGGACATGGACAGCAGCGTCAGCCCGACCCATGGTGAG
CAGGAAGGCACCGCCTACAACGGCCATTTCGGCTGCACCTGCTACCATCCGCTGTTCGTGTTCAACCAGTTCGGCGAGCTGGAGCGAACCGCGCTGCGCT
CTGGGAACGTCCACAGCGCCGATGATTGGCGCTCGGTGCTGGAGCCGGTGGTGGCGCGGTATCGGGACAGGACCAGGCGGCGGTTCTTCCGGGGCGATGC
CGCCTTCGCCCTGCCTGAGTTGTACGACTACCTGGAAGCCGAGGGCTACAAGTACACCATCCGCCTCAAGGCCAACACGGTCCTCCAGGGCCACATCGCC
CATCTGCTCAAACGCCCGGTCGGTCGTCCTCCGAAATACGTCCAGCGGTTCTACGCCAGCTTCAGCTATCAGGCCAAGTCATGGAGCCGGAAGCGCCGCG
TGGTCGCCAAGGTCGAATGGTATTCCGGCGAACTGTATCCACGTGTCGGCTTCATTGTCACCAATCTGACCCGCCCCGCCGCCCGCGTGACCGCCTTCTA
CAACCATCGTGGCACGGCGGAGCAGTACATCAAGGAGGGCAAGAACGCGGTGAAGTGGACCCGGCTGTCCTGCCGGACGATGAAGGCCAACGCGGTGCGC
CTTCAGCTTCACGCCCTGGCCTACAACCTCGCCAACTTCCTGCGCACCCTGGCCCTGCCGGACGAAATGGAAAGCTGGTCGCTCACCACTATCCGCGAGA
AGGTGGTGAAGATCGGTGCGAAGATCATCGCCCACGCCCGCTACAGTGTCTTCCAGATGGCCGAGGTCGCGGTGCCGCGTGACCTGTTCCGACGCATCCT
CGACATGATCGACAATCTGCGAGCACGAGAACCGGCGCCATGCTGACAGCAGGGTGGATCCGCACAAGGCAAGCGACAGGCGAAGTGCGCTCGGGATCCA
GCGAAATGAGCCAAAATCGCGCTGATGAAGCCAGCCATGGAGGTCGGGTTGACGATCATGGTGCGGCCGAGGCGGCCACTGTGACGATCCGCCTTCTGGC
AGGCCCGGACGTGGGTTATCCTGCCATCAAACAGGGGCGGTTCGGCCGCTCAAATGGGAAATGTCGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1353 bp450 aa941446+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEMAAGETESADLRVAFDRRLKLEFHGSRITSDAGLLAFRELDDALGLTEMAGQVLADSRTGKNNRHTLTAQFRQSVFGRLAGYEDVNDADRLGHDPAMR
WIVGGRAVTKEAASTSQMGRFETEVLTGKANLTALADLSGKWIDAVHARRPTNVVVLDMDSSVSPTHGEQEGTAYNGHFGCTCYHPLFVFNQFGELERTA
LRSGNVHSADDWRSVLEPVVARYRDRTRRRFFRGDAAFALPELYDYLEAEGYKYTIRLKANTVLQGHIAHLLKRPVGRPPKYVQRFYASFSYQAKSWSRK
RRVVAKVEWYSGELYPRVGFIVTNLTRPAARVTAFYNHRGTAEQYIKEGKNAVKWTRLSCRTMKANAVRLQLHALAYNLANFLRTLALPDEMESWSLTTI
REKVVKIGAKIIAHARYSVFQMAEVAVPRDLFRRILDMIDNLRAREPAPC

 

Blast result :
Comments
ISApr8 is 77% aa similar to ISCARN79.
References
1] ISfinder annotation (2009)
2] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.