ISDth1
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ACJN01000002 | ND | Desulfonatronospira thiodismutans | Desulfonatronospira thiodismutans ASO3-1 |
DNA section
IS Length : 1673 bp
Ends
IR Length : 14/17
IRL : CCTGATTCCGTGAACTTGTTGCATAAACATTGCAGTAACAATGCAGTTTT
IRR : CCTGAATCCGTGACGTTATATACATAGACCCCTGCGCAGCCCTCCGAGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCCCCTGAATGCTTAC | TGAAATGCTAACGCCAACCA | 0 |
DNA sequence
CCTGATTCCGTGAACTTGTTGCATAAACATTGCAGTAACAATGCAGTTTTTTGTTGACTTTATCCATAAATTTCAGTAGGTTCCATCACTAATGCAACTT
TTTTGAAACTGCACCCAACAGGTGATGGAGGCTACAATAATGAAGATATCTAAAGTGGTTAATAAGGATGACCTGCAGACAGGCCCGGCCGGCCTGATTT
TGCTGGGCGAAATGGTCAGGGCGATAGAACTGGACAAAATAGCTAAAGCCATTACTTCCGGTAATCCCCAGATAACCGCGGCAGATATCCTGAGAACTAT
GATCGGCCTTTTGAGCCAGGGCAAGTCCGACTTTGACCACGCCAAAGCTGTTATCGGTGACGAGTTCTTTATGAACTCCATGAGCATTGATCGTGTACCT
TCTGCTGAAACCTACAGGCAGAAGTTTTAGGAGCTGGCTTTGAATAAGGATCTGGAGACTATTCTGCCCGAGCTTTCCATCAAACTCTGGAAAAAGTCCG
GGGTAGAAAATGAATACATCAAGAAGGACCAAAAGAAGTGGGTGCGTGTGGACATTGACCCGGTCATCTACGACAATGCCGACACCAAGAAAGAAGGTGC
TGCCTATACCTACAACAACCAGTTCGGGTTTTCTCCTGTTTTTGCTCATTTTGCCGGGGGCTGGATGATCAATGCCAAGCTCTATCCCGGCAATTTTTCT
ACTCATAGCCGGGAAGCCATAGACTTTGTCCTGCAGAGCCTTGGTTTTGCAGACAGGATGGTCGAGGAGACCAAGCTCCTGGTGATGGATTGTGGCTTTG
ACAATCAGCAGATGCTTCAAAACCTTTGGAATCGCCCGAATACCGACTTTCTGATCAAGCACAATATAAGACGTGAGAGCCTTGAAAACTGGCTTGAAGT
AGCCAAGGAGAAGGGAGAGCTGGTATTTTCGGATGAGAAGAAAGGATATGACCTGTATCGAGGCAGCACTTATCGCCACATTGACGGCTGCGATGAGCCT
GTGCGCCTTGTTTTCGAAGTCCGGGAAATCTTCAAGAAGAAAGGGCAGCTTTTGCTCATGCCCCAGGCCAAGGTGTTCAGTGTCTGGACCTCTCTTGACC
AGGCCTTTTCTGACAAGGAAGTGCTCAAGCTCTACCGGATGCGGGGAACCAGTGAGCAGTTTCACTCCGAGTTCAAGTCCGAGATGGACATGGAGCGCTT
GCCCTCGGGAAAGTTCCAGGTGAACAGTGCCTTTTTGCTTATGGGCATGCTGACCTATAATATGCTCAAGGTTGTTGCAGAGGACATGGTAGTCCAAAAG
GCCCTGGGACTTAAGAAAGCCACCAGGCGCAGAATCAAGACCGTGATGACCAGTGTGGTCTTCCTCTCCTGCCGTCTGGTGAAGCATGCCCGGCAGCTGA
TCCTGCAGCTGGGATGTTCCAGGCCGTGGTTTGACTGGTTCGCCTCGCTGTTCACACGTCTCAAGCAGGCGTAAGGCCAGGAGGAGGCGCATGGATAAAA
CACCCAGGGAGTAGTGGGTCCAAAATCTGGAAAAATATGAATATTTGAGGCTTATCATGCTTATTTATCGCTAAGTGCCCTGTTTTTGCCCATTCCGGAA
GGCTCGGATGGACCGTAAACGCCCTCTCGGAGGGCTGCGCAGGGGTCTATGTATATAACGTCACGGATTCAGG
TTTTGAAACTGCACCCAACAGGTGATGGAGGCTACAATAATGAAGATATCTAAAGTGGTTAATAAGGATGACCTGCAGACAGGCCCGGCCGGCCTGATTT
TGCTGGGCGAAATGGTCAGGGCGATAGAACTGGACAAAATAGCTAAAGCCATTACTTCCGGTAATCCCCAGATAACCGCGGCAGATATCCTGAGAACTAT
GATCGGCCTTTTGAGCCAGGGCAAGTCCGACTTTGACCACGCCAAAGCTGTTATCGGTGACGAGTTCTTTATGAACTCCATGAGCATTGATCGTGTACCT
TCTGCTGAAACCTACAGGCAGAAGTTTTAGGAGCTGGCTTTGAATAAGGATCTGGAGACTATTCTGCCCGAGCTTTCCATCAAACTCTGGAAAAAGTCCG
GGGTAGAAAATGAATACATCAAGAAGGACCAAAAGAAGTGGGTGCGTGTGGACATTGACCCGGTCATCTACGACAATGCCGACACCAAGAAAGAAGGTGC
TGCCTATACCTACAACAACCAGTTCGGGTTTTCTCCTGTTTTTGCTCATTTTGCCGGGGGCTGGATGATCAATGCCAAGCTCTATCCCGGCAATTTTTCT
ACTCATAGCCGGGAAGCCATAGACTTTGTCCTGCAGAGCCTTGGTTTTGCAGACAGGATGGTCGAGGAGACCAAGCTCCTGGTGATGGATTGTGGCTTTG
ACAATCAGCAGATGCTTCAAAACCTTTGGAATCGCCCGAATACCGACTTTCTGATCAAGCACAATATAAGACGTGAGAGCCTTGAAAACTGGCTTGAAGT
AGCCAAGGAGAAGGGAGAGCTGGTATTTTCGGATGAGAAGAAAGGATATGACCTGTATCGAGGCAGCACTTATCGCCACATTGACGGCTGCGATGAGCCT
GTGCGCCTTGTTTTCGAAGTCCGGGAAATCTTCAAGAAGAAAGGGCAGCTTTTGCTCATGCCCCAGGCCAAGGTGTTCAGTGTCTGGACCTCTCTTGACC
AGGCCTTTTCTGACAAGGAAGTGCTCAAGCTCTACCGGATGCGGGGAACCAGTGAGCAGTTTCACTCCGAGTTCAAGTCCGAGATGGACATGGAGCGCTT
GCCCTCGGGAAAGTTCCAGGTGAACAGTGCCTTTTTGCTTATGGGCATGCTGACCTATAATATGCTCAAGGTTGTTGCAGAGGACATGGTAGTCCAAAAG
GCCCTGGGACTTAAGAAAGCCACCAGGCGCAGAATCAAGACCGTGATGACCAGTGTGGTCTTCCTCTCCTGCCGTCTGGTGAAGCATGCCCGGCAGCTGA
TCCTGCAGCTGGGATGTTCCAGGCCGTGGTTTGACTGGTTCGCCTCGCTGTTCACACGTCTCAAGCAGGCGTAAGGCCAGGAGGAGGCGCATGGATAAAA
CACCCAGGGAGTAGTGGGTCCAAAATCTGGAAAAATATGAATATTTGAGGCTTATCATGCTTATTTATCGCTAAGTGCCCTGTTTTTGCCCATTCCGGAA
GGCTCGGATGGACCGTAAACGCCCTCTCGGAGGGCTGCGCAGGGGTCTATGTATATAACGTCACGGATTCAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 140 | 1474 | + | No |
Chemistry : DDE
ORF sequence :
MKISKVVNKDDLQTGPAGLILLGEMVRAIELDKIAKAITSGNPQITAADILRTMIGLLSQGKSDFDHAKAVIGDEFFMNSMSIDRVPSAETYRQKF*ELA
LNKDLETILPELSIKLWKKSGVENEYIKKDQKKWVRVDIDPVIYDNADTKKEGAAYTYNNQFGFSPVFAHFAGGWMINAKLYPGNFSTHSREAIDFVLQS
LGFADRMVEETKLLVMDCGFDNQQMLQNLWNRPNTDFLIKHNIRRESLENWLEVAKEKGELVFSDEKKGYDLYRGSTYRHIDGCDEPVRLVFEVREIFKK
KGQLLLMPQAKVFSVWTSLDQAFSDKEVLKLYRMRGTSEQFHSEFKSEMDMERLPSGKFQVNSAFLLMGMLTYNMLKVVAEDMVVQKALGLKKATRRRIK
TVMTSVVFLSCRLVKHARQLILQLGCSRPWFDWFASLFTRLKQA
LNKDLETILPELSIKLWKKSGVENEYIKKDQKKWVRVDIDPVIYDNADTKKEGAAYTYNNQFGFSPVFAHFAGGWMINAKLYPGNFSTHSREAIDFVLQS
LGFADRMVEETKLLVMDCGFDNQQMLQNLWNRPNTDFLIKHNIRRESLENWLEVAKEKGELVFSDEKKGYDLYRGSTYRHIDGCDEPVRLVFEVREIFKK
KGQLLLMPQAKVFSVWTSLDQAFSDKEVLKLYRMRGTSEQFHSEFKSEMDMERLPSGKFQVNSAFLLMGMLTYNMLKVVAEDMVVQKALGLKKATRRRIK
TVMTSVVFLSCRLVKHARQLILQLGCSRPWFDWFASLFTRLKQA
Blast result :
Comments
ISDth1 is 52% aa similar to ISAzo40.
There is a STOP codon in frame at position 97 of the transposase.
There is a STOP codon in frame at position 97 of the transposase.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Muyzer,G. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Muyzer,G. (2009) Direct submission GenBank.