ISAar7

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 2983 bp

Ends


IR Length : 22/27

IRL : TGTGTGTGATCAACTTTAAGTAAGCCATCACGGCTGAAGAAATAGGCCAT
IRR : TGTGTTTCGTCAAGTTTAAGTAGGCCATTAAAGCGCTAAAAAGTAGGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TCCACCGTATGTCTTCACTGCCGGAA6
CCCGGCGCCAACCCCACAAA0
CGCGTAAAGGCAGACTCGGAGCGCTAG7

DNA sequence

TGTGTGTGATCAACTTTAAGTAAGCCATCACGGCTGAAGAAATAGGCCATTAAAGCGGCCACATTTGAGCCATTAAAGCGGTAAAAGTAGGCCACCTACG
CCACCGGGTCTGCCAAGAATCCTTCGCAGCCTCGACCGTGGCCACCACGTTGATCACCACACCCAGACGGTTCCCGCCCTTTGCTTGTCGTTCCTGTCAC
GAAGAAGACAGCAAAGGACATGGGCCCGTCTGGCCAGATAAGTTCCTTACGGCGCCCCATTTTTGCCTATGTACGGCGCCGGAAGGTCTGGAAGGAAATG
ACGGAACCCATGTCCGTGCAAGAAACTATCAGGAAATTAGACTCAGAGGGTATCTCCGGGCGAAAAATCGCCGTTCAGCTGAACCTCAGCCGAGCCACTG
TCGCCAAATACTTGAGCATCACTAACTATTCACCGGCCCCACCCAGAACGGGTCAACGTCCCGCGGGCTCAGTCATCACAGGCTTCGAGGAGACTATCAG
TACCTGGCTCGAAGGCGACAGAGGCCGGCCACGCAAGCAACGCCATACCGCCCAACGCGTCTTTGACCGGCTCGTGGATGAAGAAAACTATCAAGGCACG
TATTCACCGGTGCAGCGATTCATCAAACGATATAAGCAGGAACGCCAAAGCGACAACGACGGATTCCTCGAGCTGGATTGGGCTCCGGGCACCATTCAAG
TGGACTTCGGCGAAGCTGAGGTCATCTTGAATGGCGAACAAAAAGTCGTGCACCTTTTCGTAGTTACCTTCCCGTATTCGAATATGCGCTTCGCGCAGGC
TTACGGCGGGCAGACTGCTGAATGCGTGTGCCACGGGTTGCGGACCGTCTTCGAACACATCGGTTCGGTGCCGCATCGGATGATCTTTGATAACGCCACC
GGAGTGGGGCGCAGGGTCAAAACCAAGGTGCTGGAAACGAAATTATTTGCTGCGTTCAAAGCGCATTACCGGTCCGAGGCGAAGTATTGCAATCCATATT
CCGGTAATGAGAAGGGCAATGTTGAAAATGCGGTCGGTTTCTTGCGCCGCAATCTGATGGTTCCACTGCCTGTTGCAGCGAGCTTGGAAGGGCTCAATAA
AGTACTGCTCGAACGTTGTGACCGGCTCGGAGACAAGCCGCATTGGCGGCGCAAGATTCTGATCAAGGACCTCTTCGCCCAAGATGTTCAGGCTGGTTTG
GCGTTACCGGGGGTAGGTTTTGATCCGGTGCGTTACGAGAGCCGTAAGGCCGATAAATACGGCCACCTGAAAGTGGGATCCAATACGTATGCCGCTGGCC
CGGTGTTCGCTAGGAGGTCGTTGACGGTCGGGATCCGTCACGACGTCGTGGAGATCCTCGACGAACAAGCCTCGGTGTTGAGAAGGTTTCCCCGAATTTT
CGGGGATCACCCGGAAATAATCATGGAACCATCCGGGGTGCTCGCGTTGCTGGCTCACCGTCCGGGAGCGTGGGCTAACTCCCCAGTGCGGGCGGTGGTT
TCTGACCCCGTGCGTGACTGGATGGATACCGCGCACACCGAGGACCGGCGCCGGCTACTGACCGCGCTGGACAAGGCCAGCGAGACTGCCGGGTTTGAAA
CCGCAATACAGGCAGCAGGGCAGATCATTGAGCAAGGTGATGATCCTGGCCATGAAACGTTAAGCATGCTCGCCAGGAGGTTGGCCGAAGGTACCGAACC
GGAAACTGGGGATGTTGACCTGTCTGTTTATGATCGGCTCTTCGCAGCCCAGGAAGAATCGGTGTTGGCATGAATCCGGTGAGCGTTGAAGAGTTGGTGC
AGGCTGGACGGGCGGCTTCGATGACCGCTTCGGTGATCTCTGAGTGGGCGCATAAAGGCACCCCGAAGCAGCGTGAATTCCTTTATGAGCTGCTCCAAGC
CGAGCATGAGTCCCGCACGGCGTCGCGTCATCAACGGTTGTTGAAGGCTGCGAAGTTGCCGGCGTTGAAATCGTTGGTGGGCTATGACTGGACCTCGGTG
ACCTTCCCGACTGATTATGGGCGGGAAGCGTTGAGTGATTTGGATTTCTTGGACCATGCCCAGGATTTGGTGCTGTTCGGCGACGTGGGTACCGGCAAAA
CGCATTTGGCCACTGCGTTGGCTGCCGCTGCGTGCCTGCGGGGTATTCCGGCGAGGTTCTTCACGACTGCGTCGCTGGTGATGACGCTGCGCCGGGCGAA
AGATGAGGGGCGGTTGGATAAGGAGCTCGGCTCCATCGCGAAGAATCAGCTCCTTGTCATTGACGAATTTGGGTATCTTCCGATCGATAGTGACGGGGCC
AGGCTGCTTTTCCAGGTCATTGCTGACGGGTATGAGAAACGCAGTCTGGTGTTGACAACAAATTTGGAGTTCAAACGTTGGGGTACCGTCTTTGGCGATG
ACAATATGGCTGCCGCGGTCATTGACCGGATTGTTCATCACGGGAGGCTGTTGCAGTTCCGTGGGCAGTCGTACCGGGTGAAGCACGCGTTGATGAAATG
AGCGTAGATCATGATGAACCAGTTGAGGAACATAGCGGAGAACACGTTGAGGAAGGGCAGGTGATGGGCGTGCAGATACCGGGGCGGTGGCATCCTGAAG
GGCTCACTTTGGAGGAGGATTTGGCGTTGCTGGCCAGCGGGGAAGGAACTGGCTGGTGGGATGAGTCCGGCCGGCCAGCTCCATGGCCACAGGATTTTCT
TGACCCTGCTGCGGGCTGGTCCCAAGACGGGGTGCTGGCTGTTGACTGGGTGCAAGGTGCCCAGGTTCCGGAAGTGAAGCCCCTGGAGCAGTTACTGGAT
GAGTGGGACGGTGCCGAGCCACCGTTCTAAGTCGTGCACTATTGCCGTGGCAGGCCATGGATCGTTGTGTTTACGGGGTTTTTGGCCTGCCGCGGTGGCG
TTAGCGCTGTTATGGTCTATTTCTGCGCTGAGATGGCCTACTTTTTAGCGCTTTAATGGCCTACTTAAACTTGACGAAACACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1464 bp487 aa3101773+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSVQETIRKLDSEGISGRKIAVQLNLSRATVAKYLSITNYSPAPPRTGQRPAGSVITGFEETISTWLEGDRGRPRKQRHTAQRVFDRLVDEENYQGTYSP
VQRFIKRYKQERQSDNDGFLELDWAPGTIQVDFGEAEVILNGEQKVVHLFVVTFPYSNMRFAQAYGGQTAECVCHGLRTVFEHIGSVPHRMIFDNATGVG
RRVKTKVLETKLFAAFKAHYRSEAKYCNPYSGNEKGNVENAVGFLRRNLMVPLPVAASLEGLNKVLLERCDRLGDKPHWRRKILIKDLFAQDVQAGLALP
GVGFDPVRYESRKADKYGHLKVGSNTYAAGPVFARRSLTVGIRHDVVEILDEQASVLRRFPRIFGDHPEIIMEPSGVLALLAHRPGAWANSPVRAVVSDP
VRDWMDTAHTEDRRRLLTALDKASETAGFETAIQAAGQIIEQGDDPGHETLSMLARRLAEGTEPETGDVDLSVYDRLFAAQEESVLA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
732 bp243 aa17702501+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNPVSVEELVQAGRAASMTASVISEWAHKGTPKQREFLYELLQAEHESRTASRHQRLLKAAKLPALKSLVGYDWTSVTFPTDYGREALSDLDFLDHAQDL
VLFGDVGTGKTHLATALAAAACLRGIPARFFTTASLVMTLRRAKDEGRLDKELGSIAKNQLLVIDEFGYLPIDSDGARLLFQVIADGYEKRSLVLTTNLE
FKRWGTVFGDDNMAAAVIDRIVHHGRLLQFRGQSYRVKHALMK

 

Blast result :
Comments
ISAar7 is 61%(istA) and 73%(istB) aa similar to ISBad1.
References
1] ISfinder annotation (2009).