ISfinder Mirror Site

ISBth7

Family IS1182
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_005957	ND	Bacillus thuringiensis	Bacillus thuringiensis sv. konkukian 97-27

DNA section

IS Length : 1560 bp

Ends

IR Length : 14/20

IRL : CAGGCTGTGGAGAAAGTCTTCTCCACAGCCTGTTTTTGTGTCTGAACCTC
IRR : CAGAGTGTTGGGAAACCCTTTAGGGTTCCCAACACTCTGGAATCTTTTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAAAATGAAAAAAC	AAAAAACGTAGCCAAAAAGCTACGTTTTTCT	TTCATCGTTTCGG	31

DNA sequence

CAGGCTGTGGAGAAAGTCTTCTCCACAGCCTGTTTTTGTGTCTGAACCTCTTTTTATGTGTCAACACTGATAAAAAAGAGGAAAAGGAGAGATTTTTTAT
GATGGGTGCTTTAAAAAATCATCGTGATGAGCGTGTATCTGTTTCTGTAGAAGAGTTGGTTCCACAAGATCATTTCCTTCGTGCAATTGAAGCGACAATT
AGTTTTGATTTTATTGAAGAGAAGTTACGCCCTTATTACTGTGAAAACAATGGACGCCCGTCCATTCATCCTATTGTTTTATTTAAGATGATGTTTATTG
GATATTTTTACGGTATTCGTTCTGAGCGTCAATTGGAAAAGGAAATTAAAACGAATATTGCCTACCGTTGGTTTTTAGGGTTTTCTCTATCGGATACTAT
TCCGGATCACTCTACAATCAGTTGGAATCGTCGTACTCGTTTTATTCATACAAATATTTTCGAAGAAATTTTTGATGAGATTGTGAGACAAGCGACGGAG
CACCGGATGGTAGGAGGACGTGCACTGATGACAGATTCTACACATATTAAGGCCAATGCCAATAAAAATAAATTTGTACATAAATATAAAAAAGAAAAAG
CAAAGTTTTATATAGACGAGCTAGAAGCTGCGGTAATCGAAGATCGTGAGGTACATGGAAAAAAGAATTAAAACCTAGAAAGGAAAGTGATACCCCTACA
AAAAAAACTCGTATAAGTACAACGGATCCAGATAGTGGATTTTTAATGAGAGATGGAAAACCAAATGGTTTTCATTTCTTGGATCATCGTACGACTGATG
CGAAGTACAATATTATTACAGATACCTATGTAACGGCTGGAAATATGGCAGATTCCGAGCCGTATTTAGCACGCCTCCAAGCTCAGATTGATAAATTCGA
CTTCAAAGTAGAAGCTGTTGCCCTTGATGCTGGCTATTTTACAGGATATATCTGTAAGAAACTATCAGAACGGAATATATTTATGGTGATGGGGTATCGC
CGATTTGGAAAACGGAATAAAGAAGTACCGAAAAGCCAATTTAAATATGTAGAAGAAATGAATGTTTTCGCTTGTCCGATGGGATGTATTCTAGAATATG
TAACAACTGATCGTGAAGGATACCGTCAATACAAATCGAATCCAGAAGACTGCGCTGTTTGTCCGTTACGTGAGGTGTGCTTTTCAAAGAAACAAAAACA
AAAAGTTATCACTCATCATGTGTGGGAGAAATATAAGGATATTGCTAGAAAAAACAAGCTAACAGCTACAGGGAAAAGGTTGTATAAAGTACGCTGTTCC
ACTATAGAGCGAAGCTTTGCGGACGCCAAAGAACTACATGGCTACCGCTATGCCCGGTTTCGAGGGCTGAAGTCTGTCCAAATGCAAGCTTATCTCACCG
CAGCTTGTCAAAACATGAAAAAAATAGCCCTCCACCTGACCAAAAAGGGTCAGGTGGAGGGCTATTTTTTTTAATTCTATTATTTTATGTTGTTTTTATT
CGCTAAAATCAAAAAAGATTCCAGAGTGTTGGGAACCCTAAAGGGTTTCCCAACACTCTG

Recoding section

Recoding by frameshift
Frame -1
Type
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
573 bp	190 aa	99	671	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MMGALKNHRDERVSVSVEELVPQDHFLRAIEATISFDFIEEKLRPYYCENNGRPSIHPIVLFKMMFIGYFYGIRSERQLEKEIKTNIAYRWFLGFSLSDT
IPDHSTISWNRRTRFIHTNIFEEIFDEIVRQATEHRMVGGRALMTDSTHIKANANKNKFVHKYKKEKAKFYIDELEAAVIEDREVHGKKN

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
825 bp	274 aa	650	1474	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GTWKKELKPRKESDTPTKKTRISTTDPDSGFLMRDGKPNGFHFLDHRTTDAKYNIITDTYVTAGNMADSEPYLARLQAQIDKFDFKVEAVALDAGYFTGY
ICKKLSERNIFMVMGYRRFGKRNKEVPKSQFKYVEEMNVFACPMGCILEYVTTDREGYRQYKSNPEDCAVCPLREVCFSKKQKQKVITHHVWEKYKDIAR
KNKLTATGKRLYKVRCSTIERSFADAKELHGYRYARFRGLKSVQMQAYLTAACQNMKKIALHLTKKGQVEGYFF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1376 bp	458 aa	99	1474	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMGALKNHRDERVSVSVEELVPQDHFLRAIEATISFDFIEEKLRPYYCENNGRPSIHPIVLFKMMFIGYFYGIRSERQLEKEIKTNIAYRWFLGFSLSDT
IPDHSTISWNRRTRFIHTNIFEEIFDEIVRQATEHRMVGGRALMTDSTHIKANANKNKFVHKYKKEKAKFYIDELEAAVIEDREVHGKKELKPRKESDTP
TKKTRISTTDPDSGFLMRDGKPNGFHFLDHRTTDAKYNIITDTYVTAGNMADSEPYLARLQAQIDKFDFKVEAVALDAGYFTGYICKKLSERNIFMVMGY
RRFGKRNKEVPKSQFKYVEEMNVFACPMGCILEYVTTDREGYRQYKSNPEDCAVCPLREVCFSKKQKQKVITHHVWEKYKDIARKNKLTATGKRLYKVRC
STIERSFADAKELHGYRYARFRGLKSVQMQAYLTAACQNMKKIALHLTKKGQVEGYFF

Blast result :

Comments

ISBth7 is 53% aa similar to ISSag8.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2009)
2] Han,C.S., Xie,G., Challacombe,J.F., Altherr,M.R., Bhotika,S.S., Brown,N., Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C., Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A., Green,L.D., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P., Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., McMurry,K., Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T., Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L., Rubin,E., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N., Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Brettin,T.S. and Gilna,P. (2006) J. Bacteriol. 188 (9), 3382-3390