ISAzo2

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006824 ND Azoarcus sp.
Azoarcus sp. EbN1 plasmid 2
DNA section
IS Length : 1545 bp

Ends


IR Length : 13/14

IRL : CAGCCTGTCGGGCTTTCACAGAGATAGGCGATAATCCCAGCCAGACGAAC
IRR : CAGCCTGTCGGACTACGCCACCCTGGGTGGGGTAGTGTTCGCGATGCGGA

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNCTAGNNNNNNNNNN4

DNA sequence

CAGCCTGTCGGGCTTTCACAGAGATAGGCGATAATCCCAGCCAGACGAACTGACGGAATCGGCTGCGATGGCGTTCCACCCGATAGATCGAGACACAGAC
TTTCTGTTGCCGCCCTCGGTGCAGGACTGGCTGCCGGAAGCGCATCTGGCGCGGTACGTGGTGGACGTCGTCGAGCAGCTGGATTTGTCGGCGATGGAGC
GGGCGTACGCCGGCCGTGGCAGCGACGCTTATCATCCGGCAACGCTGCTGGCGCTGCTGATCTACGGCTACGCCACCGGGACCTTCTCGAGCCGCAAGAT
CGAGCGCGCGACCTACGACTCGCTGGCGTTCCGTTACATCGCCTGCAACCGGCATCCCGACCACGACACGCTGGCCAATTTCAGGAAGCGTTTCGGCGGG
GAGTTCGAAGCGGCCTTTGTCCAGGTGCTGCAGGTAGCCCGCGAGAACCAGCTCTCACGCTTTGGCACGGTGAGTCTGGACGGGACCAAGATCCACGCCA
ATGCGAGCCGGCACAGTGCGCTCTCCTATGGCCACGCACGGACGATCGAGGCGCAGCTCAAGGTCGAGGTGCAGGAGCTCATGAAGCTGGCCGCGGCGGC
CGATCAATCCTCGCTGCCGGACGGGGTGAAGCTGCCCGACGAGATTCGTCGTCGCGAGGACCGGCTAGCGGCGATCGCGGCGGCGAAGGCCAAGATCGAA
GCGCGTGCCGCGGAGCGCTTCGAACGCGAACAGGCCGAGCACCAGGCCAAGCTCGCGAAGCGGCAGGCGCAGGAAGCCAAGACCGGCAAGAAGCCGGGGG
GCAAGCCGCCGAAGCCGCCGCAAGCCGGTCCGCGCGCCGACGATCAGATCAACCTGACCGACGAAGAGTCCCGGATCATGCCGGTGTCGGGCGGCGGATT
CGAGCAGAGCTACAACGCGCAGGCGGTGGTCGATACTGAGTCGATGCTCATCCTCGCCACTCACGTGACCCAGGCGACCAACGACAAGGAACAGGTCGCG
CCGATGCTCGCGAAAGTCCAGTCCAATCCCGAGGGGTTGAACCGGCCGGCGACCTGGCTCGCTGACACCGGTTACTACAGCGAAAAGAACGTCACGGCGT
GCGTCGCCGCGAAGATCGAACCGCTGATCGCGGTCAAGCGCGACGAGCACCATCCGGATTGGCGGGAGCGTTTCAGCGAACCTGAACCGTTGGCCGCAGG
CGCCTCGGCGGTCGAGACGATGAAGCACAAGCTCAAGACCCGGGCCGGCCGCGCGGCCTATGCGCTGCGTAAGCAAACGGTCGAGCCGGTATTCGGCATC
ATCAAGTCGGTGATGGGCTTCCGCCAGTTTCTGCTGCGCGGCCTTGATAACGTGACAAACGAATGGACCCTCGTTTGCCTCGCGTGGAATTTGAAGCGCA
TGGCCGTATTGCGTCCGCAGTAAGCAAACGGACGGGGAATCGTGCGCGTTCTCGCAGAAATCACGATGAAAACGGCCTTCAGGCCCGATTCGCGTTCCGC
ATCGCGAACACTACCCCACCCAGGGTGGCGTAGTCCGACAGGCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1356 bp451 aa681423+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAFHPIDRDTDFLLPPSVQDWLPEAHLARYVVDVVEQLDLSAMERAYAGRGSDAYHPATLLALLIYGYATGTFSSRKIERATYDSLAFRYIACNRHPDHD
TLANFRKRFGGEFEAAFVQVLQVARENQLSRFGTVSLDGTKIHANASRHSALSYGHARTIEAQLKVEVQELMKLAAAADQSSLPDGVKLPDEIRRREDRL
AAIAAAKAKIEARAAERFEREQAEHQAKLAKRQAQEAKTGKKPGGKPPKPPQAGPRADDQINLTDEESRIMPVSGGGFEQSYNAQAVVDTESMLILATHV
TQATNDKEQVAPMLAKVQSNPEGLNRPATWLADTGYYSEKNVTACVAAKIEPLIAVKRDEHHPDWRERFSEPEPLAAGASAVETMKHKLKTRAGRAAYAL
RKQTVEPVFGIIKSVMGFRQFLLRGLDNVTNEWTLVCLAWNLKRMAVLRPQ

 

Blast result :
Comments
ISAzo2 is 88% aa similar to ISAzo1.
There are 2 copies in Azoarcus sp. EbN1 plasmid 2.
References
1] ISfinder annotation (2005)
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36