ISBcl1
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006582 | ND | Bacillus clausii | Bacillus clausii KSM-K16 |
DNA section
IS Length : 1536 bp
Ends
IR Length : 11/15
IRL : CAGCCTGTCGACAAAGTCTCGGTTTTACCGAGACAGTCGACAGGCTTTTT
IRR : CAGATCGTAGACAAAACGCCTTTGAGAAAATAATTCTCAAAGGCGTTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTTGGAGCTTGATAAAAA | AGGCATTGCCGCATGAGGCAATGCCT | TTTTTATGTAGCCAAATTCC | 26 |
DNA sequence
CAGCCTGTCGACAAAGTCTCGGTTTTACCGAGACAGTCGACAGGCTTTTTTGTACAATAAAAGAGAGAAAAAAAGGTACAGGGTGAGGAAGAATGTTGTC
CAAACAGTTGGAAGATAAGCGAATGCAAATGGAAATGGTCTGGATTGAAAAGCTGGTGCCAGAGGATCATCTCGTCCGGAAGCTTGATGCGGCGATTGAC
TTTGATTTCATTTATGACCTTGTCGAAGACCTTTACTCAAAAGACAGGGGCCGGCCGAGTGTTGATCCCGTGGTGTTGATCAAAATGGTTTTCATTCAAT
ACATCTTTGGCATTCGTTCGATGCGCCAAACGATTAAAGAAATCGAAACCAACGTGGCCTACCGTTGGTTTTTAGGATTTGGCTTTGAGGATAAGATCCC
CCACTTTTCTACGTTTGGGAAGAACTACGTTCGTCGTTTCCGGGACAGTGATGTGTTTGAACAACTCTTTTATCGGATTCTGAAACAAGCTATGGACAAA
GGATTTGTGAGTCCAGAGGTGGCTTTTATCGACTCGACTTCCATTAAAGCCAACGCCAATAAACGAAAGCATCGCAAAAAGGTCGTACGCACCGAGACGC
GTGCCTATCAAACTCAACTAGAAGAAGAAATCAATGGGGAACGGGTGAAAAACGGAAAAAAGCCCTTACCTCCCTCGCAAAAAGACCCGTCGAGGGAGGT
CAAGGAAAGTACCACCGATCCTGAGAGCGGTTATTACGTAAAAAGCGAGCGGGAAAAGATGTTTGCCTACAATTTCCATACGGCTTGCGATGCCAATGGA
TTTGTTTTAGGAACGATCGTAAAACCGGCGAATGTCCACGATAGTCAGGTTTTCCACGAGCTGTTTGATCAATTGAAAACGAAAGTGTGCCAACCAAAGG
TTGTCGCGGTGGATGCGGGGTATAAAACGCCTGCCATTGCCAAAAAACTTCAAGAAGAAGGCGTTCATCCCGTCATGCCATATAAACGCCCGATGACCCC
TAAAGGCTATATCCGGAAAAACGAGTTTGTCTATGATGAATACTACGATTGTTTTCTTTGTCCCCAAAACCAGGTGCTTTCTTACCGAACAACGAATCGA
GAGGGATATCGTGAATATATCTCCGACCCTCGGATTTGTGAGTCGTGCCCACTGCTCGCCCAGTGCACAAAGAATCGGAATCACCAAAAGCTCGTCCTTC
GCCATATTTGGCAGGATGCCTTGGATGAAGCAGAACATCTGAGGCATACTGAACGGAACCGAGACATCTACGCCAAGCGGAAAGAAACGATTGAGCGTGT
CTTTGCCGATCTGAAACAGAAGCATGGTCTGCGTTGGACCAACCTAAAAGGGTTGGAAAAAAATGCAATGCAGGCGATGCTTGTTTTTGGTGCGATGAAC
TTGAAAAAATTAGCCACCTGGAGTTGGAGGAGGAATCTTCCTCCAACTCCAAAGTCTCCAATATCCACCCATCTCTTTATCATCAAAAAAACGCCTTTGA
GAATTATTTTCTCAAAGGCGTTTTGTCTACGATCTG
CAAACAGTTGGAAGATAAGCGAATGCAAATGGAAATGGTCTGGATTGAAAAGCTGGTGCCAGAGGATCATCTCGTCCGGAAGCTTGATGCGGCGATTGAC
TTTGATTTCATTTATGACCTTGTCGAAGACCTTTACTCAAAAGACAGGGGCCGGCCGAGTGTTGATCCCGTGGTGTTGATCAAAATGGTTTTCATTCAAT
ACATCTTTGGCATTCGTTCGATGCGCCAAACGATTAAAGAAATCGAAACCAACGTGGCCTACCGTTGGTTTTTAGGATTTGGCTTTGAGGATAAGATCCC
CCACTTTTCTACGTTTGGGAAGAACTACGTTCGTCGTTTCCGGGACAGTGATGTGTTTGAACAACTCTTTTATCGGATTCTGAAACAAGCTATGGACAAA
GGATTTGTGAGTCCAGAGGTGGCTTTTATCGACTCGACTTCCATTAAAGCCAACGCCAATAAACGAAAGCATCGCAAAAAGGTCGTACGCACCGAGACGC
GTGCCTATCAAACTCAACTAGAAGAAGAAATCAATGGGGAACGGGTGAAAAACGGAAAAAAGCCCTTACCTCCCTCGCAAAAAGACCCGTCGAGGGAGGT
CAAGGAAAGTACCACCGATCCTGAGAGCGGTTATTACGTAAAAAGCGAGCGGGAAAAGATGTTTGCCTACAATTTCCATACGGCTTGCGATGCCAATGGA
TTTGTTTTAGGAACGATCGTAAAACCGGCGAATGTCCACGATAGTCAGGTTTTCCACGAGCTGTTTGATCAATTGAAAACGAAAGTGTGCCAACCAAAGG
TTGTCGCGGTGGATGCGGGGTATAAAACGCCTGCCATTGCCAAAAAACTTCAAGAAGAAGGCGTTCATCCCGTCATGCCATATAAACGCCCGATGACCCC
TAAAGGCTATATCCGGAAAAACGAGTTTGTCTATGATGAATACTACGATTGTTTTCTTTGTCCCCAAAACCAGGTGCTTTCTTACCGAACAACGAATCGA
GAGGGATATCGTGAATATATCTCCGACCCTCGGATTTGTGAGTCGTGCCCACTGCTCGCCCAGTGCACAAAGAATCGGAATCACCAAAAGCTCGTCCTTC
GCCATATTTGGCAGGATGCCTTGGATGAAGCAGAACATCTGAGGCATACTGAACGGAACCGAGACATCTACGCCAAGCGGAAAGAAACGATTGAGCGTGT
CTTTGCCGATCTGAAACAGAAGCATGGTCTGCGTTGGACCAACCTAAAAGGGTTGGAAAAAAATGCAATGCAGGCGATGCTTGTTTTTGGTGCGATGAAC
TTGAAAAAATTAGCCACCTGGAGTTGGAGGAGGAATCTTCCTCCAACTCCAAAGTCTCCAATATCCACCCATCTCTTTATCATCAAAAAAACGCCTTTGA
GAATTATTTTCTCAAAGGCGTTTTGTCTACGATCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1444 bp | 481 aa | 93 | 1536 | + | No |
Chemistry : DDE
ORF sequence :
MLSKQLEDKRMQMEMVWIEKLVPEDHLVRKLDAAIDFDFIYDLVEDLYSKDRGRPSVDPVVLIKMVFIQYIFGIRSMRQTIKEIETNVAYRWFLGFGFED
KIPHFSTFGKNYVRRFRDSDVFEQLFYRILKQAMDKGFVSPEVAFIDSTSIKANANKRKHRKKVVRTETRAYQTQLEEEINGERVKNGKKPLPPSQKDPS
REVKESTTDPESGYYVKSEREKMFAYNFHTACDANGFVLGTIVKPANVHDSQVFHELFDQLKTKVCQPKVVAVDAGYKTPAIAKKLQEEGVHPVMPYKRP
MTPKGYIRKNEFVYDEYYDCFLCPQNQVLSYRTTNREGYREYISDPRICESCPLLAQCTKNRNHQKLVLRHIWQDALDEAEHLRHTERNRDIYAKRKETI
ERVFADLKQKHGLRWTNLKGLEKNAMQAMLVFGAMNLKKLATWSWRRNLPPTPKSPISTHLFIIKKTPLRIIFSKAFCLRS
KIPHFSTFGKNYVRRFRDSDVFEQLFYRILKQAMDKGFVSPEVAFIDSTSIKANANKRKHRKKVVRTETRAYQTQLEEEINGERVKNGKKPLPPSQKDPS
REVKESTTDPESGYYVKSEREKMFAYNFHTACDANGFVLGTIVKPANVHDSQVFHELFDQLKTKVCQPKVVAVDAGYKTPAIAKKLQEEGVHPVMPYKRP
MTPKGYIRKNEFVYDEYYDCFLCPQNQVLSYRTTNREGYREYISDPRICESCPLLAQCTKNRNHQKLVLRHIWQDALDEAEHLRHTERNRDIYAKRKETI
ERVFADLKQKHGLRWTNLKGLEKNAMQAMLVFGAMNLKKLATWSWRRNLPPTPKSPISTHLFIIKKTPLRIIFSKAFCLRS
Blast result :
Comments
ISBcl1 is 67% aa similar to ISSag8.
There is no STOP codon in the sequence, so we cut the amino sequence at the end of the IS.
There is no STOP codon in the sequence, so we cut the amino sequence at the end of the IS.
References
1] ISfinder annotation (2009)
2] Shirai,T., Suzuki,A., Yamane,T., Ashida,T., Kobayashi,T., Hitomi,J.
and Ito,S. (1997) Protein Eng. 10 (6), 627-634
3] Kobayashi,T., Hakamada,Y., Adachi,S., Hitomi,J., Yoshimatsu,T., Koike,K., Kawai,S. and Ito,S. (1995) Appl. Microbiol. Biotechnol. 43 (3), 473-481
4] Hakamada,Y., Kobayashi,T., Hitomi,J., Kawai,S. and Ito,S. (1994) J. Ferment. Bioeng. 78, 105-108
2] Shirai,T., Suzuki,A., Yamane,T., Ashida,T., Kobayashi,T., Hitomi,J.
and Ito,S. (1997) Protein Eng. 10 (6), 627-634
3] Kobayashi,T., Hakamada,Y., Adachi,S., Hitomi,J., Yoshimatsu,T., Koike,K., Kawai,S. and Ito,S. (1995) Appl. Microbiol. Biotechnol. 43 (3), 473-481
4] Hakamada,Y., Kobayashi,T., Hitomi,J., Kawai,S. and Ito,S. (1994) J. Ferment. Bioeng. 78, 105-108