ISAzba5
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum brasilense | Azospirillum brasilense |
DNA section
IS Length : 840 bp
Ends
IR Length : 16/20
IRL : GGGTCTGGACCCATAAAGAGCCTTTCCCGAAGGGAATCGTTATGATTCAA
IRR : GGGTCCGGACTCATAACCAGTAGGCGACGGTTGCCACGATGTGGACGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCTTA | TAACCAACTC | 0 |
DNA sequence
GGGTCTGGACCCATAAAGAGCCTTTCCCGAAGGGAATCGTTATGATTCAAAGCCCTGGAAAGGGGCTTTGTGATGAACGACGGTCAGTTCTGGTTGACGG
TGGAGCAGTTCGGACGGCTTGAGCCGCACCTTCCGCGCGATACCCGAGGCAAGCCGCGCGTTGATGACCGACGTGTGATCAGCGGGATCGTCCATGTGCT
GAAGTCGGGTGGGCGCTGGGCGGACGCGCCGCCGGTCTACGGTCCGCGCAAGACGCTCTACAACCGCTTCGTCCGCTGGGCGGCCAAGGGCGTGTGGGAG
GATATCTTCCATGCGTTGGCAGCGGCAGGTGGCCCACCGGCCCAGGTGATGATCGACTCCACGGCGGTCCGTGCCCATCGTTCGGCGAGTGGCGGGAAAG
GGGGGAGCGCGCCCAGGCCATCGGACGGTCCCGTGGCGGACGAACCACCAAAATCCACGCCCTGAGCGATCCGCGCGGTCGGCCGCTCGCCTTCCTGTTG
ACCGGCGGTCAGGTTGCCGACTGCACCGCCGCCGACCGTCTGCTCGACCGGATGCCCGCCACCGATCTCCTGCACGGCGACAAGGGATACGACAGTGCCG
CTGTTCGCCGGAAGATCGAAGAGGCGGGAGCCGCGCCCAACATCCCGCCACGCGCCAACAAGCGCTGGAAAAACTGCTTCTCTCCCTACCTCTATCGGAA
CCGCAACGTCATCGAGCGCATGTTTGGGCGCCTCAAGGACTTCCGGCGCATCGCCACCCGCTATGACCGCTCCGCCACCAACTTCATGGCCGCCGTCCAC
ATCGTGGCAACCGTCGCCTACTGGTTATGAGTCCGGACCC
TGGAGCAGTTCGGACGGCTTGAGCCGCACCTTCCGCGCGATACCCGAGGCAAGCCGCGCGTTGATGACCGACGTGTGATCAGCGGGATCGTCCATGTGCT
GAAGTCGGGTGGGCGCTGGGCGGACGCGCCGCCGGTCTACGGTCCGCGCAAGACGCTCTACAACCGCTTCGTCCGCTGGGCGGCCAAGGGCGTGTGGGAG
GATATCTTCCATGCGTTGGCAGCGGCAGGTGGCCCACCGGCCCAGGTGATGATCGACTCCACGGCGGTCCGTGCCCATCGTTCGGCGAGTGGCGGGAAAG
GGGGGAGCGCGCCCAGGCCATCGGACGGTCCCGTGGCGGACGAACCACCAAAATCCACGCCCTGAGCGATCCGCGCGGTCGGCCGCTCGCCTTCCTGTTG
ACCGGCGGTCAGGTTGCCGACTGCACCGCCGCCGACCGTCTGCTCGACCGGATGCCCGCCACCGATCTCCTGCACGGCGACAAGGGATACGACAGTGCCG
CTGTTCGCCGGAAGATCGAAGAGGCGGGAGCCGCGCCCAACATCCCGCCACGCGCCAACAAGCGCTGGAAAAACTGCTTCTCTCCCTACCTCTATCGGAA
CCGCAACGTCATCGAGCGCATGTTTGGGCGCCTCAAGGACTTCCGGCGCATCGCCACCCGCTATGACCGCTCCGCCACCAACTTCATGGCCGCCGTCCAC
ATCGTGGCAACCGTCGCCTACTGGTTATGAGTCCGGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 393 bp | 130 aa | 73 | 465 | + | No |
Description : First part of the transposase
ORF sequence :
MNDGQFWLTVEQFGRLEPHLPRDTRGKPRVDDRRVISGIVHVLKSGGRWADAPPVYGPRKTLYNRFVRWAAKGVWEDIFHALAAAGGPPAQVMIDSTAVR
AHRSASGGKGGSAPRPSDGPVADEPPKSTP
AHRSASGGKGGSAPRPSDGPVADEPPKSTP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 563 bp | 220 aa | 168 | 730 | + | No |
Description : Second part of the transposase
ORF sequence :
PTCDQRDRPCAEVGWALGGRAAGLRSAQDALQPLRPLGGQGRVGGYLPCVGSGRWPTGPGDDRLHGGPCPSFGEWRERGERAQAIGRSRGGRTTKIHALS
DPRGRPLAFLLTGGQVADCTAADRLLDRMPATDLLHGDKGYDSAAVRRKIEEAGAAPNIPPRANKRWKNCFSPYLYRNRNVIERMFGRLKDFRRIATRYD
RSATNFMAAVHIVATVAYWL
DPRGRPLAFLLTGGQVADCTAADRLLDRMPATDLLHGDKGYDSAAVRRKIEEAGAAPNIPPRANKRWKNCFSPYLYRNRNVIERMFGRLKDFRRIATRYD
RSATNFMAAVHIVATVAYWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 658 bp | 252 aa | 73 | 730 | + | Yes |
Chemistry : DDE
ORF sequence :
MNDGQFWLTVEQFGRLEPHLPRDTRGKPRVDDRRVISGIVHVLKSGGRWADAPPVYGPRKTLYNRFVRWAAKGVWEDIFHALAAAGGPPAQVMIDSTAVR
AHRSASGGKGGERAQAIGRSRGGRTTKIHALSDPRGRPLAFLLTGGQVADCTAADRLLDRMPATDLLHGDKGYDSAAVRRKIEEAGAAPNIPPRANKRWK
NCFSPYLYRNRNVIERMFGRLKDFRRIATRYDRSATNFMAAVHIVATVAYWL
AHRSASGGKGGERAQAIGRSRGGRTTKIHALSDPRGRPLAFLLTGGQVADCTAADRLLDRMPATDLLHGDKGYDSAAVRRKIEEAGAAPNIPPRANKRWK
NCFSPYLYRNRNVIERMFGRLKDFRRIATRYDRSATNFMAAVHIVATVAYWL
Blast result :
Comments
ISAzba5 is 83% aa similar to ISSen2. The third ORF is a putative ORFAB transposase recontructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)