ISAar6

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1519 bp

Ends


IR Length : 32/36

IRL : GAGCCTGTCAGGTGGATTGTGTAAGACCCCTAGTCAATTGATAGAAAGAG
IRR : GAGCCTGTCAGATAGTTTGTGTAAGCCCCTAGTCACGTCCGCTATTCAGG

Insertion site


Left flankDirect repeatRight flankDR Length
GTTTTTGGCCTGCCGCGGTGGCGTTAGC8

DNA sequence

GAGCCTGTCAGGTGGATTGTGTAAGACCCCTAGTCAATTGATAGAAAGAGACTAGGAAAAAACACGATATGACCATCCAGAAAGACCAGGCCGAACAGAG
CATGGAAAACGCCTCAGACATCGCCGAGAAGATCGCAGCCCTCGGCTCCATGGACGCCGTGAAGGAATCCGGGGCCTTCGACGAGCTCATGGGATTCATC
GACCGCGGAGAGATCCAGCTCGACGGCAAGAACGGCTTCATCCAGCAGATCATCCGCGCCGGCCTGGAACGCGGCCTGAAATCCGAGCTGAGCAGCCACC
TGGGCTACGAGCAAGGCGAAAGCCATGGGCGATTCCTCCCGAACTCCCGCAACGGCTCCTACGCGAAGACCGTGTCCACAGTGGCCGGGGATATCGACCT
GCAGATCCCCAGGGACCGTGCCGGCACCTTTGTCCCGACCCTGGTGCCCAAGGGCTCACGCCGCACCGGCGGCCTGGATGAAATGATCATTTCCCTCTAC
GCCGGCGGCATGACCGTCCGGGATATCGCCTACCACCTAGAAACCACCGTCGGCACCCAGCTGTCCCACGACACGATCTCCAAGATCACCGACGAAGTCC
TCGACGAGGTCGCTGCTTGGCAGGAACGCCCGCTGGAAGAGTTCTACCCGATCCTCTACCTGGATGCGATTGTCATCAAGGTCCGCGACGGGCACCAGGT
GCGGAACAAAGCCGCGCATATGGCCATCGGCGTCACCGTGGAAGGCATCAAGCACGTGCTGGGCCTGTGGGTCACCGAGAATGAAGGGGCGAAGTTCTGG
GCGCAAGTCTGCGCGCAGCTGGCTAACCGGGGTGTGAAGGATGTCCTGATTGCCTGCTGTGACGGCCTGACCGGTTTCCCCGAAGCGATCGAAGCGACCT
GGTCGCGGGCCACGGTGCAGACCTGTGTGGTGCACTTGATCCGGGCTGCGAACCGGTTCGTGTCCTATACCGATCGTCGTGCGGTCAGCGCCCAGTTGAA
GAGCATTTACACGGCTCCGAGCATTGAATCCGCGTGCAAGGCGTTGGATGATTTCGAAGCGTCCCAGCTGGGCCAGAAATATCCGTCAGCGGTGGCGACA
TGGCGCAACGCGTGGGAGCGCTTCACCCCGTTCTTGGAGTTCCCTCCGGAGCTGCGCAAGGTCATTTACACGACCAATGCGATCGAATCAATGAACTACC
AGTTGCGCAAGGTCACCAAGTCCCGGGGCCATTTCCCGTCGGACGTGGCCGCGGTGAAGATGCTCTGGTTGGCGATCTGCAATATCGAGGACAAGCGGGC
CCGGGAGCGAGCCAAAGACGCGCAGAAGAAGGGGCGGACCCCGCGGGCCGGAGCCGGTTCGCACCTGGTTGAAGGGGCCGGGACGAGTGGCTGGAAGCAA
GCGTTGCAGCAGTTGGCTATGGCTTACCCTGAACGTATTGAGCCGTATTTGAAGTAATTGTTACGAAGCCCTGAATAGCGGACGTGACTAGGGGCTTACA
CAAACTATCTGACAGGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1389 bp462 aa691457+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTIQKDQAEQSMENASDIAEKIAALGSMDAVKESGAFDELMGFIDRGEIQLDGKNGFIQQIIRAGLERGLKSELSSHLGYEQGESHGRFLPNSRNGSYAK
TVSTVAGDIDLQIPRDRAGTFVPTLVPKGSRRTGGLDEMIISLYAGGMTVRDIAYHLETTVGTQLSHDTISKITDEVLDEVAAWQERPLEEFYPILYLDA
IVIKVRDGHQVRNKAAHMAIGVTVEGIKHVLGLWVTENEGAKFWAQVCAQLANRGVKDVLIACCDGLTGFPEAIEATWSRATVQTCVVHLIRAANRFVSY
TDRRAVSAQLKSIYTAPSIESACKALDDFEASQLGQKYPSAVATWRNAWERFTPFLEFPPELRKVIYTTNAIESMNYQLRKVTKSRGHFPSDVAAVKMLW
LAICNIEDKRARERAKDAQKKGRTPRAGAGSHLVEGAGTSGWKQALQQLAMAYPERIEPYLK

 

Blast result :
Comments
ISAar6 is 75% aa similar to IS1132.
ISAar6 was reconstructed in silico by deletion of ISAar1 sequence and one of the direct repeat generated by its insertion.
The transposase is not affected by insertion of ISAar1.
References
1] ISfinder annotation (2009).