ISAar5

  • Family IS256
  • Group IS1249
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
DNA section
IS Length : 1341 bp

Ends


IR Length : 24/27

IRL : GGGCTATAGGCAATAAAGCATGTCTGAAAACCTGATCCTGCCCCGGAAAG
IRR : GGGGCTATAGGCAATAAAGTGTGTCTGGCCCGGGCGTGTTCCCGGGCCAG

Insertion site


Left flankDirect repeatRight flankDR Length
ACAAGAGCTTGTATTACGGCATTAATG7
GGGCTATGGCGCATGTGCGCCATAGCC7
TCGGTAATCGGGAAAGTAGATTGCACCC8
AAGACTCTGCTGCTATCGTTCCTAGCCA8

DNA sequence

GGGCTATAGGCAATAAAGCATGTCTGAAAACCTGATCCTGCCCCGGAAAGCCGGGGCAGGATCAAGGTTCACATGGGTATTGAATCCATAAGTGTGAAGC
TTACTGGACAATCCCATACTTGTATTATCTGTCATTCCCGACTGAAGAAAAACGGAACCACCTCCAAAGGAACCCAGCGCTGGCGCTGCATCACCTGCAA
TGCCAGCACCGTTTTCAAACGCGAAGACACCACCGCCCGCAACCAACTCACCGGCTTCGTCTCCTGGCTGATGAGCAAACATTCCCAAGCCGAATTCGGC
GGCGGAACCGGCCGCACCTTCCGGCATCAGACCGCTTGGTGCTGGAACGTGCACCCCTACCTGCATCACACCGGCGAGGTCTTCGACGAGATCCAAGTCG
ACGGCATCTATCTGCGCCAAGGCTGGTGCCTGCTGATCGCCATCGCTCACGGACGTGTCATCGGATGGCAGTGGTGCGACCGCGAAAAAGCCGAGGCGTG
GACCGTGTTGGTGCAGCATTTCCCCGAACCGAAAGTCGTGGTGACCGACGGCGGATCAGGCTTGATGAAAGCGCTCAAAGAGTTCTGGCCGAACGTGAAA
ATTCAACGCTGCTTGGTGCATATCCAGCGCAACGTGCGCACCTACCTGACCCTGAATCCGCGTTTGCCTGCAGGGAAGTCGTTACGTCGTTTGAGCCTGA
AACTCACGAAAATCCGGACCCAGGAAGCTGCGGCAGAATGGATTGCCGCCTTCGCTGCGTGGCATGCCGAAAACCACGAGCTGATCAATGAACGCACCTA
CGCTGCTGACTGGTCCGGTGCCTGGCCGCGGGGCCTGCGACGCAACCGCAAGTGGTGGTACACCCACGATCGGCTACGCAGCGCCTACGAGTCGATGAAC
AAAGCCCTGCGACGCGGGCATCTGTTCACCTACCTGGACAAGGACCTGAACGGCTTGGGAATCAACGCGACGACGAACATGATTGAAGGTGCGGTGAATT
CCGGCATCCGGGCCATGATTTTCTATCACCGCGGGATGCCCATTGAGCATCGCCGGCGGGCGTGCGAGTGGTTCTGCTGGACTCATGCGGATGAAGGAAA
CCGCCCGGCCTTGCGGACACTGTTGCGTCCGGAGCATTACACTCCCGAAACGAAGAAGAAGGCGCAGCACATGGTTGATGAAGCACCAATCGGCCCGGAG
CTTTATGGCACCGGCCCCAGCGCTGAGGACGGGCAATTCATTCGCAGTGGTTGGATGAGAAATATCTACTAGAGCAGGGTTTTAAGGTTGTCTGGCCCGG
GAACACGCCCGGGCCAGACACACTTTATTGCCTATAGCCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1200 bp399 aa731272+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGIESISVKLTGQSHTCIICHSRLKKNGTTSKGTQRWRCITCNASTVFKREDTTARNQLTGFVSWLMSKHSQAEFGGGTGRTFRHQTAWCWNVHPYLHHT
GEVFDEIQVDGIYLRQGWCLLIAIAHGRVIGWQWCDREKAEAWTVLVQHFPEPKVVVTDGGSGLMKALKEFWPNVKIQRCLVHIQRNVRTYLTLNPRLPA
GKSLRRLSLKLTKIRTQEAAAEWIAAFAAWHAENHELINERTYAADWSGAWPRGLRRNRKWWYTHDRLRSAYESMNKALRRGHLFTYLDKDLNGLGINAT
TNMIEGAVNSGIRAMIFYHRGMPIEHRRRACEWFCWTHADEGNRPALRTLLRPEHYTPETKKKAQHMVDEAPIGPELYGTGPSAEDGQFIRSGWMRNIY

 

Blast result :
Comments
ISAar5 is 52% aa similar to IS1249.
References
1] ISfinder annotation (2009).