ISAar4
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 Arthrobacter arilaitensis plasmid pRE117 |
DNA section
IS Length : 1304 bp
Ends
IR Length : 24/29
IRL : TGGGCTGCTATGGATATTTCGGACAGTGGCACCCCATGAAATGATGGGGT
IRR : TGGGCTGCTTAGGAGTTTTCGGACAGCGGATTAATGGTTTCTCTACGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGAAGAGCGT | GGC | CTAGACCAAA | 3 |
| ATTACGGGAA | GTC | TGATCACTTT | 3 |
| CCACGTCAGG | CAC | GTCATTAAGC | 3 |
DNA sequence
TGGGCTGCTATGGATATTTCGGACAGTGGCACCCCATGAAATGATGGGGTTCACCGAAATGAGGAAGCACCACAAATGTCATCAACACGACGCAAGTTCA
GCCAGGAATTCAAAGACGAACTCTGCCGCGAAGTCATCGACACAGCCAGACCCATCAAGTCTGTCGCCACTGAATACGGCGTCGGCACCGAAACGCTACG
CAACTGGCTGAAGAAGTACAAAGAAGCCCACGGCGGCCCTGAGACCGAGCCGGAACTCAACGTTTCCGAACGTGCCCGACTCAAGGAACTCGAACGCGAA
AACCAAGAGCTGAAGGCCGAAGCAGCGTTCCTAAAAAAAGCGGCCGCATACTTCGCAAGGGAGCCACGGTAGTGGCGAAGTATGAATTCATCGACTCCTA
CGCAGCAGACTCGTCAACTCCGGCGATTGTGAAGATGTGCCGATGGCTGGAGGTCTCCCGGTCCGGGTTCTACCACTGGCGCTCCCGCCCAGTCTCGGCC
ACCGCGGCTCGCAGACAGGCTTTGGCGGACCGTGTGAGCCATTTCTTCGAGGAATCAGAAGGAACCTACGGGTACCGCAGGATCCACGCGGACCTCGCCG
CTGAGAACAGCGAGTGCTCACCGGAGCTGGTCCGCCAGATCATGCGCCAAGAGGGCCTGGTGCCGTGTCAGCCACGACCGTTTAGGACCACTACCGAAGC
GGATGCCGAAGCTGCTGCAAGCATGCCTGATCTGGTGAAACGTGACTTCACCGCGAATCGCCCGGGCCTCAAGTTCGTCGGTGATATTACGTATATTCAC
ACGTGGCAGGGGTTCATCTATCTGGCGACGGTCATCGACTGCTATTCGAAGAAAGTCGTTGGCTGGGCGATCGAGGATCACATGCGTGCCGAGCTGGTGG
AGAAGGCCCTGCGCAACGCTGCGGATACGAGCAGGATCGAGCCGAACGCGATCAGCCACAGCGACCGCGGAAGTGTTTATACCTCGGCTTCATACCGGGC
TTTGGTTGCCCGGTTGGGGATGCGTTCGTCCATGGGTAAGACCGGGATTTGCTGGGACAATAGCCAGGCAGAAACTTTTTTCTCGGCGTTGAAGAACGAG
CGGGTCTACCGCACGACCTACGCTACGAAGGAACGGGCCCGCAAGGACGTTATCCGCTATATTGAGGGGTTCTATAACAGTCGTAGGCGTCATTCGGCGC
TTGGTTATCGGCGTCCGAATGAAGTCCATTATAGTTATCAGCAGTCGGCTACGGCAGCGTAGAGAAACCATTAATCCGCTGTCCGAAAACTCCTAAGCAG
CCCA
GCCAGGAATTCAAAGACGAACTCTGCCGCGAAGTCATCGACACAGCCAGACCCATCAAGTCTGTCGCCACTGAATACGGCGTCGGCACCGAAACGCTACG
CAACTGGCTGAAGAAGTACAAAGAAGCCCACGGCGGCCCTGAGACCGAGCCGGAACTCAACGTTTCCGAACGTGCCCGACTCAAGGAACTCGAACGCGAA
AACCAAGAGCTGAAGGCCGAAGCAGCGTTCCTAAAAAAAGCGGCCGCATACTTCGCAAGGGAGCCACGGTAGTGGCGAAGTATGAATTCATCGACTCCTA
CGCAGCAGACTCGTCAACTCCGGCGATTGTGAAGATGTGCCGATGGCTGGAGGTCTCCCGGTCCGGGTTCTACCACTGGCGCTCCCGCCCAGTCTCGGCC
ACCGCGGCTCGCAGACAGGCTTTGGCGGACCGTGTGAGCCATTTCTTCGAGGAATCAGAAGGAACCTACGGGTACCGCAGGATCCACGCGGACCTCGCCG
CTGAGAACAGCGAGTGCTCACCGGAGCTGGTCCGCCAGATCATGCGCCAAGAGGGCCTGGTGCCGTGTCAGCCACGACCGTTTAGGACCACTACCGAAGC
GGATGCCGAAGCTGCTGCAAGCATGCCTGATCTGGTGAAACGTGACTTCACCGCGAATCGCCCGGGCCTCAAGTTCGTCGGTGATATTACGTATATTCAC
ACGTGGCAGGGGTTCATCTATCTGGCGACGGTCATCGACTGCTATTCGAAGAAAGTCGTTGGCTGGGCGATCGAGGATCACATGCGTGCCGAGCTGGTGG
AGAAGGCCCTGCGCAACGCTGCGGATACGAGCAGGATCGAGCCGAACGCGATCAGCCACAGCGACCGCGGAAGTGTTTATACCTCGGCTTCATACCGGGC
TTTGGTTGCCCGGTTGGGGATGCGTTCGTCCATGGGTAAGACCGGGATTTGCTGGGACAATAGCCAGGCAGAAACTTTTTTCTCGGCGTTGAAGAACGAG
CGGGTCTACCGCACGACCTACGCTACGAAGGAACGGGCCCGCAAGGACGTTATCCGCTATATTGAGGGGTTCTATAACAGTCGTAGGCGTCATTCGGCGC
TTGGTTATCGGCGTCCGAATGAAGTCCATTATAGTTATCAGCAGTCGGCTACGGCAGCGTAGAGAAACCATTAATCCGCTGTCCGAAAACTCCTAAGCAG
CCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 76 | 372 | + | No |
Description : First part of the transposase
ORF sequence :
MSSTRRKFSQEFKDELCREVIDTARPIKSVATEYGVGTETLRNWLKKYKEAHGGPETEPELNVSERARLKELERENQELKAEAAFLKKAAAYFAREPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1020 bp | 339 aa | 243 | 1262 | + | No |
Description : Second part of the transposase
ORF sequence :
DRAGTQRFRTCPTQGTRTRKPRAEGRSSVPKKSGRILRKGATVVAKYEFIDSYAADSSTPAIVKMCRWLEVSRSGFYHWRSRPVSATAARRQALADRVSH
FFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQPRPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDC
YSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGSVYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERAR
KDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA
FFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQPRPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDC
YSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGSVYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERAR
KDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1187 bp | 395 aa | 76 | 1262 | + | Yes |
Chemistry : DDE
ORF sequence :
MSSTRRKFSQEFKDELCREVIDTARPIKSVATEYGVGTETLRNWLKKYKEAHGGPETEPELNVSERARLKELERENQELKAEAAFLKKSGRILRKGATVV
AKYEFIDSYAADSSTPAIVKMCRWLEVSRSGFYHWRSRPVSATAARRQALADRVSHFFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQP
RPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDCYSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGS
VYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERARKDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA
AKYEFIDSYAADSSTPAIVKMCRWLEVSRSGFYHWRSRPVSATAARRQALADRVSHFFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQP
RPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDCYSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGS
VYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERARKDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA
Blast result :
Comments
ISAar4 orfAB is 63% aa similar to ISFsp8.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009).