ISAar4

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrobacter arilaitensis
Arthrobacter arilaitensis RE117
Arthrobacter arilaitensis plasmid pRE117
DNA section
IS Length : 1304 bp

Ends


IR Length : 24/29

IRL : TGGGCTGCTATGGATATTTCGGACAGTGGCACCCCATGAAATGATGGGGT
IRR : TGGGCTGCTTAGGAGTTTTCGGACAGCGGATTAATGGTTTCTCTACGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
CGAAGAGCGTGGCCTAGACCAAA3
ATTACGGGAAGTCTGATCACTTT3
CCACGTCAGGCACGTCATTAAGC3

DNA sequence

TGGGCTGCTATGGATATTTCGGACAGTGGCACCCCATGAAATGATGGGGTTCACCGAAATGAGGAAGCACCACAAATGTCATCAACACGACGCAAGTTCA
GCCAGGAATTCAAAGACGAACTCTGCCGCGAAGTCATCGACACAGCCAGACCCATCAAGTCTGTCGCCACTGAATACGGCGTCGGCACCGAAACGCTACG
CAACTGGCTGAAGAAGTACAAAGAAGCCCACGGCGGCCCTGAGACCGAGCCGGAACTCAACGTTTCCGAACGTGCCCGACTCAAGGAACTCGAACGCGAA
AACCAAGAGCTGAAGGCCGAAGCAGCGTTCCTAAAAAAAGCGGCCGCATACTTCGCAAGGGAGCCACGGTAGTGGCGAAGTATGAATTCATCGACTCCTA
CGCAGCAGACTCGTCAACTCCGGCGATTGTGAAGATGTGCCGATGGCTGGAGGTCTCCCGGTCCGGGTTCTACCACTGGCGCTCCCGCCCAGTCTCGGCC
ACCGCGGCTCGCAGACAGGCTTTGGCGGACCGTGTGAGCCATTTCTTCGAGGAATCAGAAGGAACCTACGGGTACCGCAGGATCCACGCGGACCTCGCCG
CTGAGAACAGCGAGTGCTCACCGGAGCTGGTCCGCCAGATCATGCGCCAAGAGGGCCTGGTGCCGTGTCAGCCACGACCGTTTAGGACCACTACCGAAGC
GGATGCCGAAGCTGCTGCAAGCATGCCTGATCTGGTGAAACGTGACTTCACCGCGAATCGCCCGGGCCTCAAGTTCGTCGGTGATATTACGTATATTCAC
ACGTGGCAGGGGTTCATCTATCTGGCGACGGTCATCGACTGCTATTCGAAGAAAGTCGTTGGCTGGGCGATCGAGGATCACATGCGTGCCGAGCTGGTGG
AGAAGGCCCTGCGCAACGCTGCGGATACGAGCAGGATCGAGCCGAACGCGATCAGCCACAGCGACCGCGGAAGTGTTTATACCTCGGCTTCATACCGGGC
TTTGGTTGCCCGGTTGGGGATGCGTTCGTCCATGGGTAAGACCGGGATTTGCTGGGACAATAGCCAGGCAGAAACTTTTTTCTCGGCGTTGAAGAACGAG
CGGGTCTACCGCACGACCTACGCTACGAAGGAACGGGCCCGCAAGGACGTTATCCGCTATATTGAGGGGTTCTATAACAGTCGTAGGCGTCATTCGGCGC
TTGGTTATCGGCGTCCGAATGAAGTCCATTATAGTTATCAGCAGTCGGCTACGGCAGCGTAGAGAAACCATTAATCCGCTGTCCGAAAACTCCTAAGCAG
CCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
297 bp98 aa76372+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSSTRRKFSQEFKDELCREVIDTARPIKSVATEYGVGTETLRNWLKKYKEAHGGPETEPELNVSERARLKELERENQELKAEAAFLKKAAAYFAREPR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1020 bp339 aa2431262+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DRAGTQRFRTCPTQGTRTRKPRAEGRSSVPKKSGRILRKGATVVAKYEFIDSYAADSSTPAIVKMCRWLEVSRSGFYHWRSRPVSATAARRQALADRVSH
FFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQPRPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDC
YSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGSVYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERAR
KDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1187 bp395 aa761262+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSTRRKFSQEFKDELCREVIDTARPIKSVATEYGVGTETLRNWLKKYKEAHGGPETEPELNVSERARLKELERENQELKAEAAFLKKSGRILRKGATVV
AKYEFIDSYAADSSTPAIVKMCRWLEVSRSGFYHWRSRPVSATAARRQALADRVSHFFEESEGTYGYRRIHADLAAENSECSPELVRQIMRQEGLVPCQP
RPFRTTTEADAEAAASMPDLVKRDFTANRPGLKFVGDITYIHTWQGFIYLATVIDCYSKKVVGWAIEDHMRAELVEKALRNAADTSRIEPNAISHSDRGS
VYTSASYRALVARLGMRSSMGKTGICWDNSQAETFFSALKNERVYRTTYATKERARKDVIRYIEGFYNSRRRHSALGYRRPNEVHYSYQQSATAA

 

Blast result :
Comments
ISAar4 orfAB is 63% aa similar to ISFsp8.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009).