ISAar3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1258 bp
Ends
IR Length : 29/38
IRL : TGGTTGGGTCCCCGGAAAGTAGTCCAGTGACTATGTGAGTACTCGTGTCC
IRR : TGGTTGGGTCCCTTTAAGCAGTCCAGCAGCTATGCGATAGCTAACTGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCGAGGGAG | CTCG | ACCCTCGCCG | 4 |
| GCTAATCTTC | GTGT | CTCTCAGCTG | 4 |
| AACTACGGTC | AGTG | CGTTGGCCCT | 4 |
| GTGATGGAAG | CGGG | GACGGCTGTA | 4 |
DNA sequence
TGGTTGGGTCCCCGGAAAGTAGTCCAGTGACTATGTGAGTACTCGTGTCCGACAATGGACACAGGAGGAATCACAGAAACAATGGCACGCAGACACACAC
CAGACCAGGTCATCGCTAAAGTCCGCCAAGGACAAAAGATGCTCAACGACGGACACCCGATGATCGAGGTCATCAAGGAACTTCAGATCACTGAAGCTAC
TTGGTACCGCTGGATCAACCAGTACGGGTCCGAGAAAAACGCTGAAGCCAGCAAACGTACCAAGGAGCTAGAGAAGGAAAACGCGCGCCTCAAGAAGCTG
CTCGCTGATCAGATGCTCGCCAACGACATCCTTACTGAGGTTGCCAAGGGAAAATTCTAAGCCCCGAACCACGCCGCCGTGCTGTACGGATGGCGATGGA
AAAATTCGGGGCATCCGAACGCTTCGCTTGCCACGTTCTGGGCCAGAACCGCTCTGCACTACGTAAGAAAAAAACACCGATGAGCTTTGACGAAGCGCAG
CTACGAGCCGACTTGAGGGCTGTCGCGTCTAAGCATCCAACGTGGGGCTGGCGCAAAGCCCGCTGGCACTTGCTGGCCACCGAACGATGGAAAGACACTG
TCTTGAACAGCAAGCGCATCCGACGATTATGGCGAGAAGAAGGCTTGGTCTGTAAGCCCAAGCGTCGAAAGAAGCGGCGCACCGGCCCAGACGCTGGGGA
ACAGAAGCGGTTGACCGCCGAGTACCCGATGCATGTACTGAGCTTTGATTTCCAGTCGGATGTCACCTCTTGTGGCCGTCATATCCGGTTCTTCAATGTC
ATTGATGAGTACACGCGGACCGCTTTGGCGATCATTCCCCGCAGGTCTTTTAAAGCTGCTGATGTGGTGGCTGTTTTGGAGGACATCATTGCTGAAATTG
GGATCCAGCCAACGTTTGTGCGGTGTGATAACGGGCCTGAGTTCACTGCTGGCGCGCTGGTGGATTGGTGTAACACTGTGGGGGTGTCCACTGCGTTTAT
CGATCCGGGCTCACCTTGGCAGAACGGTTTTATCGAGTCGTTCAATGCGCAGTTCCGGCGTGAACAGCTTTCCGGGGAAATTATGGACACTATGGTGGAA
GCGAAGTATTTGGCTGATGAATGGAAGGACATTTACAATCATGAGCGTCCCCATGGGTCTTTGGATGGGTTGACGCCGTCCCGGTTTTGGGATGAGTGGG
TTAGAGAGAATCAGTTAGCTATCGCATAGCTGCTGGACTGCTTAAAGGGACCCAACCA
CAGACCAGGTCATCGCTAAAGTCCGCCAAGGACAAAAGATGCTCAACGACGGACACCCGATGATCGAGGTCATCAAGGAACTTCAGATCACTGAAGCTAC
TTGGTACCGCTGGATCAACCAGTACGGGTCCGAGAAAAACGCTGAAGCCAGCAAACGTACCAAGGAGCTAGAGAAGGAAAACGCGCGCCTCAAGAAGCTG
CTCGCTGATCAGATGCTCGCCAACGACATCCTTACTGAGGTTGCCAAGGGAAAATTCTAAGCCCCGAACCACGCCGCCGTGCTGTACGGATGGCGATGGA
AAAATTCGGGGCATCCGAACGCTTCGCTTGCCACGTTCTGGGCCAGAACCGCTCTGCACTACGTAAGAAAAAAACACCGATGAGCTTTGACGAAGCGCAG
CTACGAGCCGACTTGAGGGCTGTCGCGTCTAAGCATCCAACGTGGGGCTGGCGCAAAGCCCGCTGGCACTTGCTGGCCACCGAACGATGGAAAGACACTG
TCTTGAACAGCAAGCGCATCCGACGATTATGGCGAGAAGAAGGCTTGGTCTGTAAGCCCAAGCGTCGAAAGAAGCGGCGCACCGGCCCAGACGCTGGGGA
ACAGAAGCGGTTGACCGCCGAGTACCCGATGCATGTACTGAGCTTTGATTTCCAGTCGGATGTCACCTCTTGTGGCCGTCATATCCGGTTCTTCAATGTC
ATTGATGAGTACACGCGGACCGCTTTGGCGATCATTCCCCGCAGGTCTTTTAAAGCTGCTGATGTGGTGGCTGTTTTGGAGGACATCATTGCTGAAATTG
GGATCCAGCCAACGTTTGTGCGGTGTGATAACGGGCCTGAGTTCACTGCTGGCGCGCTGGTGGATTGGTGTAACACTGTGGGGGTGTCCACTGCGTTTAT
CGATCCGGGCTCACCTTGGCAGAACGGTTTTATCGAGTCGTTCAATGCGCAGTTCCGGCGTGAACAGCTTTCCGGGGAAATTATGGACACTATGGTGGAA
GCGAAGTATTTGGCTGATGAATGGAAGGACATTTACAATCATGAGCGTCCCCATGGGTCTTTGGATGGGTTGACGCCGTCCCGGTTTTGGGATGAGTGGG
TTAGAGAGAATCAGTTAGCTATCGCATAGCTGCTGGACTGCTTAAAGGGACCCAACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 82 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MARRHTPDQVIAKVRQGQKMLNDGHPMIEVIKELQITEATWYRWINQYGSEKNAEASKRTKELEKENARLKKLLADQMLANDILTEVAKGKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 339 | 1229 | + | No |
Description : Second part of the transposase
ORF sequence :
GCQGKILSPEPRRRAVRMAMEKFGASERFACHVLGQNRSALRKKKTPMSFDEAQLRADLRAVASKHPTWGWRKARWHLLATERWKDTVLNSKRIRRLWRE
EGLVCKPKRRKKRRTGPDAGEQKRLTAEYPMHVLSFDFQSDVTSCGRHIRFFNVIDEYTRTALAIIPRRSFKAADVVAVLEDIIAEIGIQPTFVRCDNGP
EFTAGALVDWCNTVGVSTAFIDPGSPWQNGFIESFNAQFRREQLSGEIMDTMVEAKYLADEWKDIYNHERPHGSLDGLTPSRFWDEWVRENQLAIA
EGLVCKPKRRKKRRTGPDAGEQKRLTAEYPMHVLSFDFQSDVTSCGRHIRFFNVIDEYTRTALAIIPRRSFKAADVVAVLEDIIAEIGIQPTFVRCDNGP
EFTAGALVDWCNTVGVSTAFIDPGSPWQNGFIESFNAQFRREQLSGEIMDTMVEAKYLADEWKDIYNHERPHGSLDGLTPSRFWDEWVRENQLAIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 82 | 1229 | + | Yes |
Chemistry : DDE
ORF sequence :
MARRHTPDQVIAKVRQGQKMLNDGHPMIEVIKELQITEATWYRWINQYGSEKNAEASKRTKELEKENARLKKLLADQMLANDILTEVAKGKILSPEPRRR
AVRMAMEKFGASERFACHVLGQNRSALRKKKTPMSFDEAQLRADLRAVASKHPTWGWRKARWHLLATERWKDTVLNSKRIRRLWREEGLVCKPKRRKKRR
TGPDAGEQKRLTAEYPMHVLSFDFQSDVTSCGRHIRFFNVIDEYTRTALAIIPRRSFKAADVVAVLEDIIAEIGIQPTFVRCDNGPEFTAGALVDWCNTV
GVSTAFIDPGSPWQNGFIESFNAQFRREQLSGEIMDTMVEAKYLADEWKDIYNHERPHGSLDGLTPSRFWDEWVRENQLAIA
AVRMAMEKFGASERFACHVLGQNRSALRKKKTPMSFDEAQLRADLRAVASKHPTWGWRKARWHLLATERWKDTVLNSKRIRRLWREEGLVCKPKRRKKRR
TGPDAGEQKRLTAEYPMHVLSFDFQSDVTSCGRHIRFFNVIDEYTRTALAIIPRRSFKAADVVAVLEDIIAEIGIQPTFVRCDNGPEFTAGALVDWCNTV
GVSTAFIDPGSPWQNGFIESFNAQFRREQLSGEIMDTMVEAKYLADEWKDIYNHERPHGSLDGLTPSRFWDEWVRENQLAIA
Blast result :
Comments
ISAar3 is 92% aa similar to ISAau1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009).