ISAde5
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011891 | ND | Anaeromyxobacter dehalogenans | Anaeromyxobacter dehalogenans 2CP-1 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 11
IRL : GAAGCTCCTCGAAACCAAGTCCAACAGGCCTCGCCGCCAAGCGAGGTCGA
IRR : GAAGCTCCTCGTTTGCTTCACGCGGCCTTGGCGACGACAGCCTCCTTCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGAGAGAAGG | ACCGTGAGTCC | 0 |
DNA sequence
GAAGCTCCTCGAAACCAAGTCCAACAGGCCTCGCCGCCAAGCGAGGTCGAGGAGCTTTCCCGTGAACGAGGTTGCCGAGCTTGAGCTGCCCACGCAAGAG
AACTTCCGGACGGATCTTCGCGCGCTCTTCCAGGGCGCGATCCGCGTCGCCTTGGAGGTCGTGCTGGAGGACGAGATCCGAGAGATGGTCGGGGCCAAGA
AGTGGGAGCGGCAGGCCCAGCGCGTCGACAGCAGGAACGGCACGTACCTGCGCCGCCTCATCACCACGATGGGCGCCGTCGACCTCGAGGTCCCGCGCGC
TCGGGAGGGCGGCTCTGCCGGCGGCGCGGTCATCGGACGGTACAAACGCCGGTCGGCGGAGATCGACGACGCGATGGTCAGCGCCTACGTCCACGGGGCC
TCGACCCGCGACATCGGCCAGATCACCGAGGCGCTCATGGGCGAGCACGTGAGCAAGTCGACGGTGAGCCGGGTGGCGAAGACCCCTGGGCACCAAGGTC
GAGGAGCTGCGGAACGCACCCATCGCCGGCCCGATCCCGTACCTCTTCCTCGATGCCACGTTCCTCGACGCCCGCTGGGCGCGGACGGTTGAGAACGTCT
CGGCGCTGGTGGCCTACGGCATCGGCCTCAATGGCCACCGCCAGCTCCTCGCCGTGACGATCGGCGCCGAGGAGTCGGAGGAGTCGTGGAGCGAGCTCCT
CGCGCAGCTCGTCGAGCGCGGCCTCTCGGGCGTCCAGCTCGTCATCGCCGACGCCCACGCGGGCATCGCGAAGGCGGTCCGGCTCCACCTGCCCGAGGCG
CCGCAGCAGCGGTGCGTCGTCCACCTGCAGCGCAACGTCGTCGGCAAGGCCCCCCAGAAGCTCAGGCCGCGGCTTGCCCGCGAGGTGTGGCAGGTGTTCG
TGGCGCCGACCAAGGCCGAGGCCCGCAAGCGGATGGCCCAGCTCGCCGCCGGCCTCGGGCGCCAGCTTCCGGAATCGATGGCGTGCCTCGAGGCCGGCTT
CGAGGCGGCCACCAAGTTCTACGCGTTCCCGAAGGAGCACTGGCACCGCATCCGCTCGACGAACGGGCTCGAGCGGCTCCACGGCGAGATCAAGCGCCGG
ACGCGGGCGGTGGGCGCCTTCCCGGACCGCGCGAGCGCCCTCCGGCTCGTCACCGCGGTCGCGCTCCAGGTCACGGCGATCTGGACCGACCGCCGCTACC
TGGACATGTCCCTGCTGAAGTCGAAGGAGGCTGTCGTCGCCAAGGCCGCGTGAAGCAAACGAGGAGCTTC
AACTTCCGGACGGATCTTCGCGCGCTCTTCCAGGGCGCGATCCGCGTCGCCTTGGAGGTCGTGCTGGAGGACGAGATCCGAGAGATGGTCGGGGCCAAGA
AGTGGGAGCGGCAGGCCCAGCGCGTCGACAGCAGGAACGGCACGTACCTGCGCCGCCTCATCACCACGATGGGCGCCGTCGACCTCGAGGTCCCGCGCGC
TCGGGAGGGCGGCTCTGCCGGCGGCGCGGTCATCGGACGGTACAAACGCCGGTCGGCGGAGATCGACGACGCGATGGTCAGCGCCTACGTCCACGGGGCC
TCGACCCGCGACATCGGCCAGATCACCGAGGCGCTCATGGGCGAGCACGTGAGCAAGTCGACGGTGAGCCGGGTGGCGAAGACCCCTGGGCACCAAGGTC
GAGGAGCTGCGGAACGCACCCATCGCCGGCCCGATCCCGTACCTCTTCCTCGATGCCACGTTCCTCGACGCCCGCTGGGCGCGGACGGTTGAGAACGTCT
CGGCGCTGGTGGCCTACGGCATCGGCCTCAATGGCCACCGCCAGCTCCTCGCCGTGACGATCGGCGCCGAGGAGTCGGAGGAGTCGTGGAGCGAGCTCCT
CGCGCAGCTCGTCGAGCGCGGCCTCTCGGGCGTCCAGCTCGTCATCGCCGACGCCCACGCGGGCATCGCGAAGGCGGTCCGGCTCCACCTGCCCGAGGCG
CCGCAGCAGCGGTGCGTCGTCCACCTGCAGCGCAACGTCGTCGGCAAGGCCCCCCAGAAGCTCAGGCCGCGGCTTGCCCGCGAGGTGTGGCAGGTGTTCG
TGGCGCCGACCAAGGCCGAGGCCCGCAAGCGGATGGCCCAGCTCGCCGCCGGCCTCGGGCGCCAGCTTCCGGAATCGATGGCGTGCCTCGAGGCCGGCTT
CGAGGCGGCCACCAAGTTCTACGCGTTCCCGAAGGAGCACTGGCACCGCATCCGCTCGACGAACGGGCTCGAGCGGCTCCACGGCGAGATCAAGCGCCGG
ACGCGGGCGGTGGGCGCCTTCCCGGACCGCGCGAGCGCCCTCCGGCTCGTCACCGCGGTCGCGCTCCAGGTCACGGCGATCTGGACCGACCGCCGCTACC
TGGACATGTCCCTGCTGAAGTCGAAGGAGGCTGTCGTCGCCAAGGCCGCGTGAAGCAAACGAGGAGCTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1192 bp | 394 aa | 62 | 1253 | + | No |
Chemistry : DDE
ORF sequence :
VAELELPTQENFRTDLRALFQGAIRVALEVVLEDEIREMVGAKKWERQAQRVDSRNGTYLRRLITTMGAVDLEVPRAREGGSAGGAVIGRYKRRSAEIDD
AMVSAYVHGASTRDIGQITEALMGEHVSKSTVSRVAKRPLGTKVEELRNAPIAGPIPYLFLDATFLDARWARTVENVSALVAYGIGLNGHRQLLAVTIGA
EESEESWSELLAQLVERGLSGVQLVIADAHAGIAKAVRLHLPEAPQQRCVVHLQRNVVGKAPQKLRPRLAREVWQVFVAPTKAEARKRMAQLAAGLGRQL
PESMACLEAGFEAATKFYAFPKEHWHRIRSTNGLERLHGEIKRRTRAVGAFPDRASALRLVTAVALQVTAIWTDRRYLDMSLLKSKEAVVAKAA
AMVSAYVHGASTRDIGQITEALMGEHVSKSTVSRVAKRPLGTKVEELRNAPIAGPIPYLFLDATFLDARWARTVENVSALVAYGIGLNGHRQLLAVTIGA
EESEESWSELLAQLVERGLSGVQLVIADAHAGIAKAVRLHLPEAPQQRCVVHLQRNVVGKAPQKLRPRLAREVWQVFVAPTKAEARKRMAQLAAGLGRQL
PESMACLEAGFEAATKFYAFPKEHWHRIRSTNGLERLHGEIKRRTRAVGAFPDRASALRLVTAVALQVTAIWTDRRYLDMSLLKSKEAVVAKAA
Blast result :
Comments
ISAde5 is 56% aa similar to ISCARN59.
The transposase was reconstructed in silico by joining (62-481; 480-1253).
The transposase was reconstructed in silico by joining (62-481; 480-1253).
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.